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The map label for this gene is manY/levF

Identifier: 15004771

GI number: 15004771

Start: 70552

End: 71358

Strand: Direct

Name: manY/levF

Synonym: CA_P0067

Alternate gene names: 15004771

Gene position: 70552-71358 (Clockwise)

Preceding gene: 15004770

Following gene: 15004772

Centisome position: 36.75

GC content: 38.66

Gene sequence:

>807_bases
GTGACTCTAAATATAATTCAAATGGGATTAGTAGTTATTGTAGCGTTTCTAGCTGGTATGGAAGGTATATTGGACGAATT
CCATTTCCATCAACCAGTAATTGCTTGTACTTTAATCGGATTAGTTACAGGTAACTTAGTACCTTGCTTAATATTAGGTG
GTACTCTTCAAATGATTGCCTTAGGTTGGGCAAATATAGGTGCTGCTGTAGCGCCTGATGCAGCTTTAGCATCTGTTGCA
TCCGCAATTATTTTAGTTCTTGGAGGACAAGGAAAAGCAGGAGTTTCTTCAGCTATTGCTATTGCTGTTCCACTAGCAGT
TGCAGGGCTATTATTACAAACTATTTGTCGTACAATTGGTATAATCATTATACATCGTATGGATGCTGCTGCTGAAGAAG
GAAATATAAGAAAAATTGAAATGTGGCATATTATTGCTATTTGCATGCAGGGTGTACGTATTGCAATTCCAGCAGCTTTG
ATTTTAGCAATTGGTGCTGGTCCTATTCGTTCATTACTTCAAGCTATGCCTCTTTGGTTGACAGATGGTTTAGCAATAGG
TGGTGGAATGGTTGTAGCTGTTGGTTATGCAATGGTAATCAATATGATGGCTACAAAAGAAGTATGGCCATTCTTCGCAA
TTGGTTTTGTGTTAGCAACAGTTTCACAAATTACACTTATCGGACTAGGTGCAATTGGTTTAGCTTTAGCTCTTCTTTAC
TTATCGCTTTCTAAACAAGGCGGCTCAGGTAAGGGTGGTGGATCAAATACTGGTGATCCATTGGGCGATATTATCGATAG
ATACTAA

Upstream 100 bases:

>100_bases
TGATTCAAAAGGAAATATGGACGAAATAATTAAAAAGGCCCAAGACGAACTAAATAAACAAAAATAATCTAACTATGTAT
ATAGTAAAGGAGGATTAACC

Downstream 100 bases:

>100_bases
GAAAGGAGAGAACACGAAAATGGCAAATGAATTAAAATTAACAAAGAAGGATCGTATTTCTGTTTGGTGGCGTTCAACTT
TCATTCAAGGTTCTTGGAAC

Product: mannose/fructose-specific phosphotransferase system component IIC

Products: protein histidine; sugar phosphate; D-glucosamine-6-phosphate [Cytoplasm]; pyruvate; glucose-6-phosphate [Cytoplasm]; N-acetyl-D-glucosamine-6-phosphate [Cytoplasm]; mannose-6-phosphate [Cytoplasm]; fructose-6-phosphate [Cytoplasm] [C]

Alternate protein names: EIIC-Sor; PTS system sorbose-specific EIIC component [H]

Number of amino acids: Translated: 268; Mature: 267

Protein sequence:

>268_residues
MTLNIIQMGLVVIVAFLAGMEGILDEFHFHQPVIACTLIGLVTGNLVPCLILGGTLQMIALGWANIGAAVAPDAALASVA
SAIILVLGGQGKAGVSSAIAIAVPLAVAGLLLQTICRTIGIIIIHRMDAAAEEGNIRKIEMWHIIAICMQGVRIAIPAAL
ILAIGAGPIRSLLQAMPLWLTDGLAIGGGMVVAVGYAMVINMMATKEVWPFFAIGFVLATVSQITLIGLGAIGLALALLY
LSLSKQGGSGKGGGSNTGDPLGDIIDRY

Sequences:

>Translated_268_residues
MTLNIIQMGLVVIVAFLAGMEGILDEFHFHQPVIACTLIGLVTGNLVPCLILGGTLQMIALGWANIGAAVAPDAALASVA
SAIILVLGGQGKAGVSSAIAIAVPLAVAGLLLQTICRTIGIIIIHRMDAAAEEGNIRKIEMWHIIAICMQGVRIAIPAAL
ILAIGAGPIRSLLQAMPLWLTDGLAIGGGMVVAVGYAMVINMMATKEVWPFFAIGFVLATVSQITLIGLGAIGLALALLY
LSLSKQGGSGKGGGSNTGDPLGDIIDRY
>Mature_267_residues
TLNIIQMGLVVIVAFLAGMEGILDEFHFHQPVIACTLIGLVTGNLVPCLILGGTLQMIALGWANIGAAVAPDAALASVAS
AIILVLGGQGKAGVSSAIAIAVPLAVAGLLLQTICRTIGIIIIHRMDAAAEEGNIRKIEMWHIIAICMQGVRIAIPAALI
LAIGAGPIRSLLQAMPLWLTDGLAIGGGMVVAVGYAMVINMMATKEVWPFFAIGFVLATVSQITLIGLGAIGLALALLYL
SLSKQGGSGKGGGSNTGDPLGDIIDRY

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitant with their translocation across the cell membrane. This system i

COG id: COG3715

COG function: function code G; Phosphotransferase system, mannose/fructose/N-acetylgalactosamine-specific component IIC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-4 domain [H]

Homologues:

Organism=Escherichia coli, GI1788121, Length=265, Percent_Identity=47.5471698113208, Blast_Score=195, Evalue=2e-51,
Organism=Escherichia coli, GI1789528, Length=250, Percent_Identity=22.8, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004700
- InterPro:   IPR018404 [H]

Pfam domain/function: PF03609 EII-Sor [H]

EC number: NA

Molecular weight: Translated: 27410; Mature: 27278

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS51106 PTS_EIIC_TYPE_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLNIIQMGLVVIVAFLAGMEGILDEFHFHQPVIACTLIGLVTGNLVPCLILGGTLQMIA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LGWANIGAAVAPDAALASVASAIILVLGGQGKAGVSSAIAIAVPLAVAGLLLQTICRTIG
HHHHHCCCHHCCHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IIIIHRMDAAAEEGNIRKIEMWHIIAICMQGVRIAIPAALILAIGAGPIRSLLQAMPLWL
HHHHCCCCCCCCCCCCEEHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHCCHHH
TDGLAIGGGMVVAVGYAMVINMMATKEVWPFFAIGFVLATVSQITLIGLGAIGLALALLY
HCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSLSKQGGSGKGGGSNTGDPLGDIIDRY
HHHHCCCCCCCCCCCCCCCHHHHHHCCC
>Mature Secondary Structure 
TLNIIQMGLVVIVAFLAGMEGILDEFHFHQPVIACTLIGLVTGNLVPCLILGGTLQMIA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
LGWANIGAAVAPDAALASVASAIILVLGGQGKAGVSSAIAIAVPLAVAGLLLQTICRTIG
HHHHHCCCHHCCHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IIIIHRMDAAAEEGNIRKIEMWHIIAICMQGVRIAIPAALILAIGAGPIRSLLQAMPLWL
HHHHCCCCCCCCCCCCEEHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHCCHHH
TDGLAIGGGMVVAVGYAMVINMMATKEVWPFFAIGFVLATVSQITLIGLGAIGLALALLY
HCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSLSKQGGSGKGGGSNTGDPLGDIIDRY
HHHHCCCCCCCCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N p -phosphohistidine; sugar; phosphoenolpyruvate; glucosamine [Periplasm]; phosphoenolpyruvate; beta-D-glucose [Periplasm]; N-acetyl-D-glucosamine [Periplasm]; mannose [Periplasm]; fructose [Periplasm] [C]

Specific reaction: protein N p -phosphohistidine + sugar = protein histidine + sugar phosphate phosphoenolpyruvate + glucosamine [Periplasm] = D-glucosamine-6-phosphate [Cytoplasm] + pyruvate phosphoenolpyruvate + beta-D-glucose [Periplasm] = glucose-6-phosphate [Cytoplasm]

General reaction: Transferring phosphorus-containing groups; Phosphotransferases with an alcohol group as acceptor [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7947968 [H]