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The map label for this gene is gcvH

Identifier: 150009756

GI number: 150009756

Start: 3836218

End: 3836598

Strand: Direct

Name: gcvH

Synonym: BDI_3171

Alternate gene names: 150009756

Gene position: 3836218-3836598 (Clockwise)

Preceding gene: 150009755

Following gene: 150009757

Centisome position: 79.73

GC content: 41.73

Gene sequence:

>381_bases
ATGAATTTTCCTGCAGATTTAAAGTACACAAAAGACCATGAGTGGATTCGTGTAGAAGGTGACGTAGCTTATGTTGGTAT
TACTGATTACGCTCAAGGCGAGCTAGGTGAGATCGTTTATGTAGACATTACGACAGAGGGGGAGACCGTCGCTAAAGAAG
AGGTGTTCGGTACGATTGAAGCTGTTAAGACTGTTTCCGATCTTTTCATGCCGGTTAGCGGAGAGGTTTTGGAAGTAAAC
GCTGAATTGGAAGACGCTCCTGAATTGGTTAATGAAGACGCTTATGGTAAAGGTTGGTTAATCAAGATTTCTCTTACGGA
CGCTTCTGAGTTGGAGGAACTATTATCCGCTGAAGACTATCAAAAATTAATCGCTAAATAA

Upstream 100 bases:

>100_bases
ATGCAGGTATATGCGGGGTTTTGTCTGGGATTTATATGTACCTTTGTAGCCTCATTAATGAGTTATATCTATTTATTCAT
CTAATAAAAACACTAAAATT

Downstream 100 bases:

>100_bases
GAATGACACCTGTTGTAAGTATTATCATGGGTAGTACCTCGGATCTACCCGTTATGGAGAAGGCGGCTAAGTTCTTGGAT
GAGATGGAGATCCCGTTCGA

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 126

Protein sequence:

>126_residues
MNFPADLKYTKDHEWIRVEGDVAYVGITDYAQGELGEIVYVDITTEGETVAKEEVFGTIEAVKTVSDLFMPVSGEVLEVN
AELEDAPELVNEDAYGKGWLIKISLTDASELEELLSAEDYQKLIAK

Sequences:

>Translated_126_residues
MNFPADLKYTKDHEWIRVEGDVAYVGITDYAQGELGEIVYVDITTEGETVAKEEVFGTIEAVKTVSDLFMPVSGEVLEVN
AELEDAPELVNEDAYGKGWLIKISLTDASELEELLSAEDYQKLIAK
>Mature_126_residues
MNFPADLKYTKDHEWIRVEGDVAYVGITDYAQGELGEIVYVDITTEGETVAKEEVFGTIEAVKTVSDLFMPVSGEVLEVN
AELEDAPELVNEDAYGKGWLIKISLTDASELEELLSAEDYQKLIAK

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=117, Percent_Identity=45.2991452991453, Blast_Score=116, Evalue=4e-27,
Organism=Homo sapiens, GI89057342, Length=117, Percent_Identity=44.4444444444444, Blast_Score=114, Evalue=3e-26,
Organism=Escherichia coli, GI1789271, Length=124, Percent_Identity=50, Blast_Score=124, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17507493, Length=115, Percent_Identity=43.4782608695652, Blast_Score=108, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17551294, Length=115, Percent_Identity=43.4782608695652, Blast_Score=108, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6319272, Length=125, Percent_Identity=41.6, Blast_Score=104, Evalue=4e-24,
Organism=Drosophila melanogaster, GI17865652, Length=119, Percent_Identity=42.0168067226891, Blast_Score=108, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_PARD8 (A6LGR3)

Other databases:

- EMBL:   CP000140
- RefSeq:   YP_001304499.1
- ProteinModelPortal:   A6LGR3
- SMR:   A6LGR3
- STRING:   A6LGR3
- GeneID:   5308320
- GenomeReviews:   CP000140_GR
- KEGG:   pdi:BDI_3171
- eggNOG:   COG0509
- HOGENOM:   HBG693789
- OMA:   TPKELRY
- ProtClustDB:   PRK01202
- BioCyc:   PDIS435591:BDI_3171-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 13978; Mature: 13978

Theoretical pI: Translated: 3.78; Mature: 3.78

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFPADLKYTKDHEWIRVEGDVAYVGITDYAQGELGEIVYVDITTEGETVAKEEVFGTIE
CCCCCCCEECCCCCEEEEECCEEEEEECCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHH
AVKTVSDLFMPVSGEVLEVNAELEDAPELVNEDAYGKGWLIKISLTDASELEELLSAEDY
HHHHHHHHHCCCCCCEEEEECCCCCCHHHHCCCCCCCCEEEEEEECCHHHHHHHHCHHHH
QKLIAK
HHHHCC
>Mature Secondary Structure
MNFPADLKYTKDHEWIRVEGDVAYVGITDYAQGELGEIVYVDITTEGETVAKEEVFGTIE
CCCCCCCEECCCCCEEEEECCEEEEEECCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHH
AVKTVSDLFMPVSGEVLEVNAELEDAPELVNEDAYGKGWLIKISLTDASELEELLSAEDY
HHHHHHHHHCCCCCCEEEEECCCCCCHHHHCCCCCCCCEEEEEEECCHHHHHHHHCHHHH
QKLIAK
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA