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The map label for this gene is yxeI [H]

Identifier: 150008184

GI number: 150008184

Start: 1813396

End: 1814520

Strand: Reverse

Name: yxeI [H]

Synonym: BDI_1550

Alternate gene names: 150008184

Gene position: 1814520-1813396 (Counterclockwise)

Preceding gene: 150008187

Following gene: 150008178

Centisome position: 37.71

GC content: 43.91

Gene sequence:

>1125_bases
TTGAAAACATCCTTTTCCTGTCGCTTGTTTTTATCAAAAATGATACTTATGAGACATTTCTTTATTTTATTTGCGATTGC
CGGCTTGCTTATTAATCCTTCCGCTGCAGAGGCTTGCACCGGTATTACCTTAAAAAGCAAGGACAGTACGACTATTGTAG
CACGTACGATAGAATGGGGTGGAAGTAATTTGAACAGCCAGTATGTCGTGGTTCCAAGAGGATACACCGAACGATCGTAT
ACTCCGAATGGGGTTGATGGAATGACTTTCACGGCACGTTATGGATATGTGGGCTTGGCAGTGGAGCAAAAGGAATTTAT
CGCCGAAGGACTCAATGAGGTTGGGTTATCGGCTGGATTGTTTTATTTTCCAAAGTACGGTGAATATGAGAAATATAATG
CCGCAGTTCGGGATAAAAGTATCTCTGATCTGCAATTAGTGTCTTGGTTGTTGGGGGAATGTTCAAATGTAGAAGAGGTG
AAAGAGGCTGTTAAGAAAGTGCATGTTATCAATATAGATCCCCGTGCGTCTACCGTTCATTGGCGGTTTGCCGAGCCGTC
GGGACGTCAACTGGTTCTTGAGATTATTAAGGGTGAGCTACACTTCTATGAGAATACGTTAGGAGTTCTGACTAATTCTC
CCAGTTTCGAGTGGCATCTGACGAACCTGAATAATTATGTTAATCTTTTCCCGGGTACGGCCCCCAATCAACAATTAGGA
AATATCGAGTTGTCTTCATTTGGTGCGGGAAGCGGTTTCCTTGGGATTCCGGGCGACGTAACTCCACCGTCACGTTTCGT
TCGTGCGGCTTTTTACCAAGCTTCGGCCCCACAACAAGAAACCGCATTGGAAACGGTCTTCCAGTGTTTCCAGATATTGA
ATAATTTTGATATTCCCGTAGGGATCGAATTCCCATTAGGGCAAGTTCCTTGCGATATACCGAGCGCTACTCAATGGACT
TCGGTTACGGATATGCGAAACCATGTGATTTATTACCGTACGATGTACAATAGCGTTATTCGGAATATAGATTTACGTTC
CATTGATTTCTCCACGGTTCGTTATCGTGCGGTATCCATGGATGGGACGAAGCGACAAGCGGTTGAAACGATCAAAATAG
AATAG

Upstream 100 bases:

>100_bases
TTCCTTTTCCTTTTACGGTCATTGAAGGGGTCGCTAGCATGTCCAATGCCGCTTGCTCCGCCGGTAATAAGTGCTGTTTG
TCTATCTAAAATTTCTTTTC

Downstream 100 bases:

>100_bases
GGCAGTGAGCTCTTTGGGGGATATCTCGTAAGTCAATAGGATAGGTGATAAAACTCAATGGAATAGGAAACATAACTCAA
TGGATTACGTTCCCTATTCT

Product: putative choloylglycine hydrolase

Products: 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate; glycine

Alternate protein names: NA

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MKTSFSCRLFLSKMILMRHFFILFAIAGLLINPSAAEACTGITLKSKDSTTIVARTIEWGGSNLNSQYVVVPRGYTERSY
TPNGVDGMTFTARYGYVGLAVEQKEFIAEGLNEVGLSAGLFYFPKYGEYEKYNAAVRDKSISDLQLVSWLLGECSNVEEV
KEAVKKVHVINIDPRASTVHWRFAEPSGRQLVLEIIKGELHFYENTLGVLTNSPSFEWHLTNLNNYVNLFPGTAPNQQLG
NIELSSFGAGSGFLGIPGDVTPPSRFVRAAFYQASAPQQETALETVFQCFQILNNFDIPVGIEFPLGQVPCDIPSATQWT
SVTDMRNHVIYYRTMYNSVIRNIDLRSIDFSTVRYRAVSMDGTKRQAVETIKIE

Sequences:

>Translated_374_residues
MKTSFSCRLFLSKMILMRHFFILFAIAGLLINPSAAEACTGITLKSKDSTTIVARTIEWGGSNLNSQYVVVPRGYTERSY
TPNGVDGMTFTARYGYVGLAVEQKEFIAEGLNEVGLSAGLFYFPKYGEYEKYNAAVRDKSISDLQLVSWLLGECSNVEEV
KEAVKKVHVINIDPRASTVHWRFAEPSGRQLVLEIIKGELHFYENTLGVLTNSPSFEWHLTNLNNYVNLFPGTAPNQQLG
NIELSSFGAGSGFLGIPGDVTPPSRFVRAAFYQASAPQQETALETVFQCFQILNNFDIPVGIEFPLGQVPCDIPSATQWT
SVTDMRNHVIYYRTMYNSVIRNIDLRSIDFSTVRYRAVSMDGTKRQAVETIKIE
>Mature_374_residues
MKTSFSCRLFLSKMILMRHFFILFAIAGLLINPSAAEACTGITLKSKDSTTIVARTIEWGGSNLNSQYVVVPRGYTERSY
TPNGVDGMTFTARYGYVGLAVEQKEFIAEGLNEVGLSAGLFYFPKYGEYEKYNAAVRDKSISDLQLVSWLLGECSNVEEV
KEAVKKVHVINIDPRASTVHWRFAEPSGRQLVLEIIKGELHFYENTLGVLTNSPSFEWHLTNLNNYVNLFPGTAPNQQLG
NIELSSFGAGSGFLGIPGDVTPPSRFVRAAFYQASAPQQETALETVFQCFQILNNFDIPVGIEFPLGQVPCDIPSATQWT
SVTDMRNHVIYYRTMYNSVIRNIDLRSIDFSTVRYRAVSMDGTKRQAVETIKIE

Specific function: Unknown

COG id: COG3049

COG function: function code M; Penicillin V acylase and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase C59 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003199 [H]

Pfam domain/function: PF02275 CBAH [H]

EC number: 3.5.1.24

Molecular weight: Translated: 41819; Mature: 41819

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSFSCRLFLSKMILMRHFFILFAIAGLLINPSAAEACTGITLKSKDSTTIVARTIEWG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEECCCCCEEEEEEEECC
GSNLNSQYVVVPRGYTERSYTPNGVDGMTFTARYGYVGLAVEQKEFIAEGLNEVGLSAGL
CCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCEEEEEECHHHHHHHHHHHHCCCCCE
FYFPKYGEYEKYNAAVRDKSISDLQLVSWLLGECSNVEEVKEAVKKVHVINIDPRASTVH
EECCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHEEEEEECCCCCEEE
WRFAEPSGRQLVLEIIKGELHFYENTLGVLTNSPSFEWHLTNLNNYVNLFPGTAPNQQLG
EEECCCCCHHHHHHHHHHHHHEECCEEEEEECCCCCEEEEECCCCEEEECCCCCCCCCCC
NIELSSFGAGSGFLGIPGDVTPPSRFVRAAFYQASAPQQETALETVFQCFQILNNFDIPV
CEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCE
GIEFPLGQVPCDIPSATQWTSVTDMRNHVIYYRTMYNSVIRNIDLRSIDFSTVRYRAVSM
EEECCCCCCCCCCCCCCCCCCHHHHHCCEEEEHHHHHHHHHCCCCCEEECCCEEEEEEEC
DGTKRQAVETIKIE
CCCHHHHEEEEECC
>Mature Secondary Structure
MKTSFSCRLFLSKMILMRHFFILFAIAGLLINPSAAEACTGITLKSKDSTTIVARTIEWG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEECCCCCEEEEEEEECC
GSNLNSQYVVVPRGYTERSYTPNGVDGMTFTARYGYVGLAVEQKEFIAEGLNEVGLSAGL
CCCCCCEEEEEECCCCCCCCCCCCCCCCEEEEECCEEEEEECHHHHHHHHHHHHCCCCCE
FYFPKYGEYEKYNAAVRDKSISDLQLVSWLLGECSNVEEVKEAVKKVHVINIDPRASTVH
EECCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHEEEEEECCCCCEEE
WRFAEPSGRQLVLEIIKGELHFYENTLGVLTNSPSFEWHLTNLNNYVNLFPGTAPNQQLG
EEECCCCCHHHHHHHHHHHHHEECCEEEEEECCCCCEEEEECCCCEEEECCCCCCCCCCC
NIELSSFGAGSGFLGIPGDVTPPSRFVRAAFYQASAPQQETALETVFQCFQILNNFDIPV
CEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCE
GIEFPLGQVPCDIPSATQWTSVTDMRNHVIYYRTMYNSVIRNIDLRSIDFSTVRYRAVSM
EEECCCCCCCCCCCCCCCCCCHHHHHCCEEEEHHHHHHHHHCCCCCEEECCCEEEEEEEC
DGTKRQAVETIKIE
CCCHHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oylglycine; H2O

Specific reaction: 3alpha,7alpha,12alpha-trihydroxy-5beta-cholan-24-oylglycine + H2O = 3alpha,7alpha,12alpha-trihydroxy-5beta-cholanate + glycine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867804; 9384377 [H]