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The map label for this gene is trmE

Identifier: 150006140

GI number: 150006140

Start: 4518275

End: 4519777

Strand: Direct

Name: trmE

Synonym: BVU_3651

Alternate gene names: 150006140

Gene position: 4518275-4519777 (Clockwise)

Preceding gene: 150006139

Following gene: 150006143

Centisome position: 87.51

GC content: 40.72

Gene sequence:

>1503_bases
ATGAAAATGATAAACCAAGATACAATCTGTGCCATTGCCACCGCCCAGGGAGGAGCCATCGGAATAATTCGTGTATCTGG
CCCAAAAGCGATTGAAATAACGTCACGCATATTTACACCCGCCACAGGCAAGCCACTGACAGAACGGGCACCATATACTC
TTACTTTCGGAAAGATTTGTTCCCCAAAGCGAAAAATTAATAATACACTTTTCCAACAAACAAGTGAAATTCCGCAAGAA
AAAAGTGAAACGTTACAAAAAACTTCGTCAATTACTTCTTCTGCAGAAGAAGTAATTGACGAAGTTTTAATTTCCTTGTT
CCGCGCCCCCCATTCTTATACCGGTGAAGACAGTACGGAAATCATGTGTCACGGTTCATCTTATATCCTGCAGCAAGTTA
TACAACTCCTGATCTATAATGGATGTCGTGCCGCTCTACCAGGTGAATACACCCAACGTGCTTTTCTAAACGGAAAAATG
GACTTGAGCCAAGCCGAAGCTGTGGCCGATCTGATAGCCTCATCGTCTGCTTCCACCCATCGCCTAGCCATGAGCCAGAT
GCGCGGAGGATTCAGTAAAGAACTTTCTAACCTGCGTAACCAATTACTTCATTTCACTTCGTTAATGGAATTAGAACTGG
ATTTCAGTGATCATGAAGAACTAGAATTCGCCAATCGTGACGAACTGAGCAGTCTAGCTACCCATATCGAACAAGTTATT
GCCCGGCTAGCTCATTCTTTCAGTGTAGGAAACGCAATCAAGAATGGCATTCCTGTAGCAATTATCGGAGAAACGAATGC
CGGAAAATCTACCTTACTGAATGCGTTGCTGAATGAAGAAAAAGCCATTGTCAGTGATATCCATGGAACAACCCGTGACG
TAATAGAGGATACGATCAATTTACAAGGTGTCACATTCCGATTTATTGATACAGCTGGAATCCGTCAAACGAACGATACA
ATAGAAAATCTAGGGATAGAACGTACTTTCCAAAAGATGGACCAAGCATATGTCATTTTATGGATGATAGACTCTACAGA
TGCCCAAAGGCGGTTCGAAGAATTAAAAGCCGACATTCTTCCACACTGCGAAGGGAAAAAGATGATTATTTTATTTAATA
AGTCAGATCTGTTACTAGCAACACAAAAAGAAGAGTTGTCAGCCATATTTGCTGACATGAAAGTGGAAAAACTATTTATC
TCAGCAAAGAAAAGGGAAAATATAACGATCCTGGAAAAGAAGTTAGTACAGGCAGCTGCCTTACCGGAAGTCAACCAAAA
CGATATTATCATTACAAATGTACGCCATTACGAAGCATTAACACGAGCATTGGATTCTATTCATAGAGTACAGGAAGGAT
TGCAATTGGAATTATCAGGAGATTTGGTAAGTGAGGATTTAAGACAATGTATCCACGAACTAAGTGAGATTGTTGCAGAA
GGAGGTATAACCTCGGAAGAAACATTGCAAAATATATTTCAAAACTTTTGCATCGGCAAATAG

Upstream 100 bases:

>100_bases
GATATTGACAGTAATCTATTAAACTTTTTTGATGAAACAGACCGCCGTTATTTAGAAGAAGTAACAGCCATTGTAGCTAA
AACATTATATTATTAACCAA

Downstream 100 bases:

>100_bases
CTAACTATCTGATTATCAGATAGTTATACAGTCAAAACCTATAATTTTCTGATAGAAAATGACTTATATAGATGGAAAAT
AAGCTGTTTTGAGAACCATA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MKMINQDTICAIATAQGGAIGIIRVSGPKAIEITSRIFTPATGKPLTERAPYTLTFGKICSPKRKINNTLFQQTSEIPQE
KSETLQKTSSITSSAEEVIDEVLISLFRAPHSYTGEDSTEIMCHGSSYILQQVIQLLIYNGCRAALPGEYTQRAFLNGKM
DLSQAEAVADLIASSSASTHRLAMSQMRGGFSKELSNLRNQLLHFTSLMELELDFSDHEELEFANRDELSSLATHIEQVI
ARLAHSFSVGNAIKNGIPVAIIGETNAGKSTLLNALLNEEKAIVSDIHGTTRDVIEDTINLQGVTFRFIDTAGIRQTNDT
IENLGIERTFQKMDQAYVILWMIDSTDAQRRFEELKADILPHCEGKKMIILFNKSDLLLATQKEELSAIFADMKVEKLFI
SAKKRENITILEKKLVQAAALPEVNQNDIIITNVRHYEALTRALDSIHRVQEGLQLELSGDLVSEDLRQCIHELSEIVAE
GGITSEETLQNIFQNFCIGK

Sequences:

>Translated_500_residues
MKMINQDTICAIATAQGGAIGIIRVSGPKAIEITSRIFTPATGKPLTERAPYTLTFGKICSPKRKINNTLFQQTSEIPQE
KSETLQKTSSITSSAEEVIDEVLISLFRAPHSYTGEDSTEIMCHGSSYILQQVIQLLIYNGCRAALPGEYTQRAFLNGKM
DLSQAEAVADLIASSSASTHRLAMSQMRGGFSKELSNLRNQLLHFTSLMELELDFSDHEELEFANRDELSSLATHIEQVI
ARLAHSFSVGNAIKNGIPVAIIGETNAGKSTLLNALLNEEKAIVSDIHGTTRDVIEDTINLQGVTFRFIDTAGIRQTNDT
IENLGIERTFQKMDQAYVILWMIDSTDAQRRFEELKADILPHCEGKKMIILFNKSDLLLATQKEELSAIFADMKVEKLFI
SAKKRENITILEKKLVQAAALPEVNQNDIIITNVRHYEALTRALDSIHRVQEGLQLELSGDLVSEDLRQCIHELSEIVAE
GGITSEETLQNIFQNFCIGK
>Mature_500_residues
MKMINQDTICAIATAQGGAIGIIRVSGPKAIEITSRIFTPATGKPLTERAPYTLTFGKICSPKRKINNTLFQQTSEIPQE
KSETLQKTSSITSSAEEVIDEVLISLFRAPHSYTGEDSTEIMCHGSSYILQQVIQLLIYNGCRAALPGEYTQRAFLNGKM
DLSQAEAVADLIASSSASTHRLAMSQMRGGFSKELSNLRNQLLHFTSLMELELDFSDHEELEFANRDELSSLATHIEQVI
ARLAHSFSVGNAIKNGIPVAIIGETNAGKSTLLNALLNEEKAIVSDIHGTTRDVIEDTINLQGVTFRFIDTAGIRQTNDT
IENLGIERTFQKMDQAYVILWMIDSTDAQRRFEELKADILPHCEGKKMIILFNKSDLLLATQKEELSAIFADMKVEKLFI
SAKKRENITILEKKLVQAAALPEVNQNDIIITNVRHYEALTRALDSIHRVQEGLQLELSGDLVSEDLRQCIHELSEIVAE
GGITSEETLQNIFQNFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=505, Percent_Identity=28.3168316831683, Blast_Score=179, Evalue=6e-45,
Organism=Homo sapiens, GI193082988, Length=537, Percent_Identity=27.56052141527, Blast_Score=168, Evalue=9e-42,
Organism=Homo sapiens, GI193082993, Length=497, Percent_Identity=27.364185110664, Blast_Score=165, Evalue=8e-41,
Organism=Escherichia coli, GI2367268, Length=510, Percent_Identity=33.3333333333333, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI87082120, Length=134, Percent_Identity=35.8208955223881, Blast_Score=76, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17507259, Length=458, Percent_Identity=27.9475982532751, Blast_Score=154, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6323665, Length=527, Percent_Identity=30.7400379506641, Blast_Score=201, Evalue=2e-52,
Organism=Drosophila melanogaster, GI45550813, Length=427, Percent_Identity=31.3817330210773, Blast_Score=189, Evalue=5e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_BACV8 (A6L6E8)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001300884.1
- ProteinModelPortal:   A6L6E8
- SMR:   A6L6E8
- STRING:   A6L6E8
- GeneID:   5304610
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_3651
- NMPDR:   fig|435590.6.peg.3392
- eggNOG:   COG0486
- HOGENOM:   HBG746795
- OMA:   WTVNQRQ
- ProtClustDB:   PRK05291
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 55502; Mature: 55502

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: BINDING 24-24 BINDING 120-120 BINDING 159-159 BINDING 500-500

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMINQDTICAIATAQGGAIGIIRVSGPKAIEITSRIFTPATGKPLTERAPYTLTFGKIC
CCCCCCCCEEEEEECCCCEEEEEEECCCCEEEEHHHHCCCCCCCCCCCCCCEEEEHHCCC
SPKRKINNTLFQQTSEIPQEKSETLQKTSSITSSAEEVIDEVLISLFRAPHSYTGEDSTE
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
IMCHGSSYILQQVIQLLIYNGCRAALPGEYTQRAFLNGKMDLSQAEAVADLIASSSASTH
EEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHH
RLAMSQMRGGFSKELSNLRNQLLHFTSLMELELDFSDHEELEFANRDELSSLATHIEQVI
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHCCCCHHHHHHHHHHHHHHH
ARLAHSFSVGNAIKNGIPVAIIGETNAGKSTLLNALLNEEKAIVSDIHGTTRDVIEDTIN
HHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHCC
LQGVTFRFIDTAGIRQTNDTIENLGIERTFQKMDQAYVILWMIDSTDAQRRFEELKADIL
CCCEEEEEEECCCCCCCCHHHHHCCHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHC
PHCEGKKMIILFNKSDLLLATQKEELSAIFADMKVEKLFISAKKRENITILEKKLVQAAA
CCCCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
LPEVNQNDIIITNVRHYEALTRALDSIHRVQEGLQLELSGDLVSEDLRQCIHELSEIVAE
CCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHC
GGITSEETLQNIFQNFCIGK
CCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKMINQDTICAIATAQGGAIGIIRVSGPKAIEITSRIFTPATGKPLTERAPYTLTFGKIC
CCCCCCCCEEEEEECCCCEEEEEEECCCCEEEEHHHHCCCCCCCCCCCCCCEEEEHHCCC
SPKRKINNTLFQQTSEIPQEKSETLQKTSSITSSAEEVIDEVLISLFRAPHSYTGEDSTE
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCE
IMCHGSSYILQQVIQLLIYNGCRAALPGEYTQRAFLNGKMDLSQAEAVADLIASSSASTH
EEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHH
RLAMSQMRGGFSKELSNLRNQLLHFTSLMELELDFSDHEELEFANRDELSSLATHIEQVI
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHCCCCHHHHHHHHHHHHHHH
ARLAHSFSVGNAIKNGIPVAIIGETNAGKSTLLNALLNEEKAIVSDIHGTTRDVIEDTIN
HHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHCC
LQGVTFRFIDTAGIRQTNDTIENLGIERTFQKMDQAYVILWMIDSTDAQRRFEELKADIL
CCCEEEEEEECCCCCCCCHHHHHCCHHHHHHHCCCEEEEEEEECCCHHHHHHHHHHHHHC
PHCEGKKMIILFNKSDLLLATQKEELSAIFADMKVEKLFISAKKRENITILEKKLVQAAA
CCCCCCEEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
LPEVNQNDIIITNVRHYEALTRALDSIHRVQEGLQLELSGDLVSEDLRQCIHELSEIVAE
CCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHHHHC
GGITSEETLQNIFQNFCIGK
CCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA