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The map label for this gene is mscL

Identifier: 150006077

GI number: 150006077

Start: 4449044

End: 4449484

Strand: Direct

Name: mscL

Synonym: BVU_3586

Alternate gene names: 150006077

Gene position: 4449044-4449484 (Clockwise)

Preceding gene: 150006072

Following gene: 150006078

Centisome position: 86.17

GC content: 44.67

Gene sequence:

>441_bases
ATGGGAAAAAGCAGCTTCTTGCAAGATTTTAAGGCGTTCGCAATGCGCGGAAACGTCATTGACATGGCAGTCGGTGTCAT
CATCGGCGGAGCCTTCGGTAAAATAGTATCTTCCATTGTGGCCGATGTCATCATGCCTCCCATCGGATTGCTGGTAGGTG
GCGTGAACTTTACAGATTTGAAACTACAATTAAAGCCGGCCGAAGTAGTGGATGGAGTTGAAAAAGCTGCCGTAACGTTG
AATTATGGCAACTTCCTACAAGCTACTTTCGACTTTATCATCATTGCTTTTTCTATATTCTTATTTATAAGATTACTTGC
CAAGCTGAATCGCAAGAAAGAAGAAGTTCCTGCTCCCGCCGCCCCACCTGCCCCTAGCAAGGAAGAGGTATTACTTACAG
AAATCCGTGACTTGTTGAAGGAACAGGCTGGCAAAAAATAA

Upstream 100 bases:

>100_bases
GGAATGTTGTTAAACGTTTAGTATTTATTTCCTAAACGCGGTGCAAAAATAAGAATTTGTTTTTATATTCGCACATAAAT
TCATATTAAAATAAAAAAAA

Downstream 100 bases:

>100_bases
AATATTATCCACACAAAAAAACGGGCTGCCCTCAAATGCTTGTCGGACAGCCCGTGATTTATATACCAGCCGACATTTCC
TAAACATTCCAATCTTTTTT

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 146; Mature: 145

Protein sequence:

>146_residues
MGKSSFLQDFKAFAMRGNVIDMAVGVIIGGAFGKIVSSIVADVIMPPIGLLVGGVNFTDLKLQLKPAEVVDGVEKAAVTL
NYGNFLQATFDFIIIAFSIFLFIRLLAKLNRKKEEVPAPAAPPAPSKEEVLLTEIRDLLKEQAGKK

Sequences:

>Translated_146_residues
MGKSSFLQDFKAFAMRGNVIDMAVGVIIGGAFGKIVSSIVADVIMPPIGLLVGGVNFTDLKLQLKPAEVVDGVEKAAVTL
NYGNFLQATFDFIIIAFSIFLFIRLLAKLNRKKEEVPAPAAPPAPSKEEVLLTEIRDLLKEQAGKK
>Mature_145_residues
GKSSFLQDFKAFAMRGNVIDMAVGVIIGGAFGKIVSSIVADVIMPPIGLLVGGVNFTDLKLQLKPAEVVDGVEKAAVTLN
YGNFLQATFDFIIIAFSIFLFIRLLAKLNRKKEEVPAPAAPPAPSKEEVLLTEIRDLLKEQAGKK

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=141, Percent_Identity=60.9929078014184, Blast_Score=172, Evalue=1e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_BACV8 (A6L685)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001300821.1
- ProteinModelPortal:   A6L685
- SMR:   A6L685
- STRING:   A6L685
- GeneID:   5304546
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_3586
- NMPDR:   fig|435590.6.peg.3334
- eggNOG:   COG1970
- HOGENOM:   HBG727604
- OMA:   GANVLAW
- ProtClustDB:   PRK00567
- HAMAP:   MF_00115
- InterPro:   IPR019823
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 15733; Mature: 15602

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xff83304)-; HASH(0x106c7af0)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKSSFLQDFKAFAMRGNVIDMAVGVIIGGAFGKIVSSIVADVIMPPIGLLVGGVNFTDL
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEE
KLQLKPAEVVDGVEKAAVTLNYGNFLQATFDFIIIAFSIFLFIRLLAKLNRKKEEVPAPA
EEEECHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
APPAPSKEEVLLTEIRDLLKEQAGKK
CCCCCCHHHHHHHHHHHHHHHHCCCH
>Mature Secondary Structure 
GKSSFLQDFKAFAMRGNVIDMAVGVIIGGAFGKIVSSIVADVIMPPIGLLVGGVNFTDL
CHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCEEEE
KLQLKPAEVVDGVEKAAVTLNYGNFLQATFDFIIIAFSIFLFIRLLAKLNRKKEEVPAPA
EEEECHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
APPAPSKEEVLLTEIRDLLKEQAGKK
CCCCCCHHHHHHHHHHHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA