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The map label for this gene is moeZ [H]

Identifier: 150006043

GI number: 150006043

Start: 4400602

End: 4401294

Strand: Direct

Name: moeZ [H]

Synonym: BVU_3547

Alternate gene names: 150006043

Gene position: 4400602-4401294 (Clockwise)

Preceding gene: 150006042

Following gene: 150006046

Centisome position: 85.23

GC content: 44.88

Gene sequence:

>693_bases
GTGGAAAGATATAACCGTCAAATTATTCTCCCTGAATTGGGAGAAAAAGGACAACAACGGCTTCAGCAGGCCAAAGTGCT
TATTGTCGGTGTAGGTGGTCTGGGATCTCCTGTTGCCTTGTACCTGACAGGAGCCGGAGTAGGTACCATCGGATTAATAG
ACGATGATGTGGTAAGTGTCAGCAACTTGCAACGTCAAGTACTTTATAGTGAAGCCGAGGTAGGCATGCCCAAAGCTATC
CAAGCCAAGAAACGTCTTGAAGCCCTGAACCATGATGTACAAATCAATGCCTATCCCACAAGACTGACTAAAGAGAATGC
GGATGAAATTATCCGTACCTACGACATCATTATAGATGGATGTGATAATTTCGCTACCCGCTATCTTATCAATGATATCT
GTGTGAAATGGGGAAAAGTTTATGTATATGGTGCCATCCGTGCCTTCGAAGGTCAAGTATCCGTTTTCAATTACCAAGGA
GGCCCCGATTACCGCCACTTTTTCCCCGACGAAACAGAAATGCTATCCATGCCCCATCCGCCCAAAGGAGTATTAGGTGT
AACCCCCGGCATAATAGGTTGTGCCGAAGCTGCTGAAGTGTTGAAGATTATAGGTGAATATGGCGAAGTGTTAAGCGGCA
AATTATGGACGATTAACGTAAAAACGATGGAAACCCATCTCATTTCCTTCTAG

Upstream 100 bases:

>100_bases
CAAGTTCACGCCAATACGATAGATTTTACCCATATTTTTAAAGGAATGGGGCTGTATAAATGATACAAAGCTCCTTTTTT
TCAAATTAACAACAAACAAA

Downstream 100 bases:

>100_bases
AGGAATCGAACCGACCCTATTCAAAAGACATTAATTCCTCAATTATGTGCCAACAGTCGAGGCATGACGACATCCGTCGT
CCGGCATCGACCGGCACATG

Product: molybdopterin biosynthesis protein

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: Sulfur carrier protein CysO adenylyltransferase; Sulfur carrier protein CysO sulfurtransferase [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MERYNRQIILPELGEKGQQRLQQAKVLIVGVGGLGSPVALYLTGAGVGTIGLIDDDVVSVSNLQRQVLYSEAEVGMPKAI
QAKKRLEALNHDVQINAYPTRLTKENADEIIRTYDIIIDGCDNFATRYLINDICVKWGKVYVYGAIRAFEGQVSVFNYQG
GPDYRHFFPDETEMLSMPHPPKGVLGVTPGIIGCAEAAEVLKIIGEYGEVLSGKLWTINVKTMETHLISF

Sequences:

>Translated_230_residues
MERYNRQIILPELGEKGQQRLQQAKVLIVGVGGLGSPVALYLTGAGVGTIGLIDDDVVSVSNLQRQVLYSEAEVGMPKAI
QAKKRLEALNHDVQINAYPTRLTKENADEIIRTYDIIIDGCDNFATRYLINDICVKWGKVYVYGAIRAFEGQVSVFNYQG
GPDYRHFFPDETEMLSMPHPPKGVLGVTPGIIGCAEAAEVLKIIGEYGEVLSGKLWTINVKTMETHLISF
>Mature_230_residues
MERYNRQIILPELGEKGQQRLQQAKVLIVGVGGLGSPVALYLTGAGVGTIGLIDDDVVSVSNLQRQVLYSEAEVGMPKAI
QAKKRLEALNHDVQINAYPTRLTKENADEIIRTYDIIIDGCDNFATRYLINDICVKWGKVYVYGAIRAFEGQVSVFNYQG
GPDYRHFFPDETEMLSMPHPPKGVLGVTPGIIGCAEAAEVLKIIGEYGEVLSGKLWTINVKTMETHLISF

Specific function: Catalyzes the conversion of the sulfur carrier protein CysO to CysO-thiocarboxylate. The reaction is thought to proceed in two steps:first, ATP-dependent activation of CysO as acyl- adenylate (CysO-COOAMP), followed by sulfur transfer to give CysO- thioca

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=225, Percent_Identity=45.7777777777778, Blast_Score=180, Evalue=8e-46,
Organism=Homo sapiens, GI38045948, Length=199, Percent_Identity=27.6381909547739, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI4885649, Length=132, Percent_Identity=31.0606060606061, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI87082356, Length=173, Percent_Identity=43.3526011560694, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1787048, Length=228, Percent_Identity=38.5964912280702, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1789177, Length=111, Percent_Identity=33.3333333333333, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17540406, Length=230, Percent_Identity=40, Blast_Score=169, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI71984476, Length=118, Percent_Identity=36.4406779661017, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI193203301, Length=147, Percent_Identity=27.891156462585, Blast_Score=71, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6321903, Length=213, Percent_Identity=41.7840375586854, Blast_Score=158, Evalue=7e-40,
Organism=Saccharomyces cerevisiae, GI6320598, Length=148, Percent_Identity=25.6756756756757, Blast_Score=66, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24582879, Length=225, Percent_Identity=44, Blast_Score=186, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24660640, Length=172, Percent_Identity=29.6511627906977, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI28573937, Length=141, Percent_Identity=29.0780141843972, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR001763
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00581 Rhodanese; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 25388; Mature: 25388

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERYNRQIILPELGEKGQQRLQQAKVLIVGVGGLGSPVALYLTGAGVGTIGLIDDDVVSV
CCCCCCEEECCCCCHHHHHHHHHCEEEEEECCCCCCCEEEEEECCCCCEEEECCCCHHHH
SNLQRQVLYSEAEVGMPKAIQAKKRLEALNHDVQINAYPTRLTKENADEIIRTYDIIIDG
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHEEEC
CDNFATRYLINDICVKWGKVYVYGAIRAFEGQVSVFNYQGGPDYRHFFPDETEMLSMPHP
CHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCEEEEEECCCCCCCCCCCCCHHEECCCCC
PKGVLGVTPGIIGCAEAAEVLKIIGEYGEVLSGKLWTINVKTMETHLISF
CCCCEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEECCC
>Mature Secondary Structure
MERYNRQIILPELGEKGQQRLQQAKVLIVGVGGLGSPVALYLTGAGVGTIGLIDDDVVSV
CCCCCCEEECCCCCHHHHHHHHHCEEEEEECCCCCCCEEEEEECCCCCEEEECCCCHHHH
SNLQRQVLYSEAEVGMPKAIQAKKRLEALNHDVQINAYPTRLTKENADEIIRTYDIIIDG
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHEEEC
CDNFATRYLINDICVKWGKVYVYGAIRAFEGQVSVFNYQGGPDYRHFFPDETEMLSMPHP
CHHHHHHHHHHHHHHHCCCEEEEEEEEEECCCEEEEEECCCCCCCCCCCCCHHEECCCCC
PKGVLGVTPGIIGCAEAAEVLKIIGEYGEVLSGKLWTINVKTMETHLISF
CCCCEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]