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The map label for this gene is thiH [H]

Identifier: 150006042

GI number: 150006042

Start: 4399393

End: 4400523

Strand: Direct

Name: thiH [H]

Synonym: BVU_3546

Alternate gene names: 150006042

Gene position: 4399393-4400523 (Clockwise)

Preceding gene: 150006041

Following gene: 150006043

Centisome position: 85.21

GC content: 46.77

Gene sequence:

>1131_bases
ATGTTTTCAGAAGAACTTGAAAAAATAGATTGGGAAGAAACCACCAAAACCATCTACTCCAAGACCGAAAGTGATGTGCT
CCGCGCTCTTGGCAAGGAACATTGCGATGTGGACGACTTTATGGCGCTCATCTCCCCCGCAGCAGCAAAATACCTGGAGC
CGATGGCGCAACTAAGCAAAAAATATACCGAAGAACGTTTCGGCAAAACCATCTCCATGTTTATACCGCTGTATATCACC
AACTCCTGCACCAATTCTTGTGTGTATTGTGGTTTCCATATCAGCAACCCGATGGCACGTACCATCCTCACCCCCGAACA
GATTGAAGATGAATACAAAGCCATCAAGAAACTGGGGCCTTTTGAGAACCTGTTACTGGTGACCGGAGAGAATCCGGCAA
AAGCCGGAGTCCCTTATCTGGCTAAAGCTTTGGACATCGCCAAAAAATATTTCAGCAATCTGAAGATAGAGGTAATGCCC
CTCAAAGCCGAAGAATATGCCGAACTGAAAGAACACGGACTGAATGGAGTCATTTGCTTTCAGGAAACTTATAACAAAGC
CCGTTATAATATATATCACCCACGCGGGATGAAATCCAAATTCGAGTGGCGGGTAAACGGCTTCGACCGCATGGGTCAGG
CAGGAGTCCACTCCATTGGCATGGGAGTACTTATCGGACTGGAAGAATGGCGCACCGATGTGACCATGATGGCTTATCAC
TTACGCTATCTTCAGAAAAAGTATTGGAAAACCAAATACAGTGTCAACTTCCCCCGTATGCGTCCGGCAGAAAACGGTGG
TTTCCAGCCGAATGTCATCATGAATGATCGTGAGTTGGCACAGCTCACTTTTGCCATGCGTATTTTCGATCACGATGTAG
ATATCTCCTACTCCACTCGTGAACCGGCACAAATCCGCGACAACATGGCAGGGCTAGGAGTGACTACCATGAGTGCTGAA
AGCAAAACTGAACCGGGGGGATACTATACCTATCCGCAAGCTTTGGAACAATTCCATGTAAGTGATGAGCGTACAGCCGT
AGAAGTAGAACACGCACTGAAATCATTAGGAAGGGAGCCAGTATGGAAAGACTGGGATGTTTCTTTTGACAAGTTCACGC
CAATACGATAG

Upstream 100 bases:

>100_bases
ATAAGAAACTTATAGCTTGAAACCTGTTTAAATGCCGAAAAAGTTTAAATAGGCTCTTATAGAAGCGAAATCATCTTATA
GAACTATAACAAAAAAAATT

Downstream 100 bases:

>100_bases
ATTTTACCCATATTTTTAAAGGAATGGGGCTGTATAAATGATACAAAGCTCCTTTTTTTCAAATTAACAACAAACAAAGT
GGAAAGATATAACCGTCAAA

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MFSEELEKIDWEETTKTIYSKTESDVLRALGKEHCDVDDFMALISPAAAKYLEPMAQLSKKYTEERFGKTISMFIPLYIT
NSCTNSCVYCGFHISNPMARTILTPEQIEDEYKAIKKLGPFENLLLVTGENPAKAGVPYLAKALDIAKKYFSNLKIEVMP
LKAEEYAELKEHGLNGVICFQETYNKARYNIYHPRGMKSKFEWRVNGFDRMGQAGVHSIGMGVLIGLEEWRTDVTMMAYH
LRYLQKKYWKTKYSVNFPRMRPAENGGFQPNVIMNDRELAQLTFAMRIFDHDVDISYSTREPAQIRDNMAGLGVTTMSAE
SKTEPGGYYTYPQALEQFHVSDERTAVEVEHALKSLGREPVWKDWDVSFDKFTPIR

Sequences:

>Translated_376_residues
MFSEELEKIDWEETTKTIYSKTESDVLRALGKEHCDVDDFMALISPAAAKYLEPMAQLSKKYTEERFGKTISMFIPLYIT
NSCTNSCVYCGFHISNPMARTILTPEQIEDEYKAIKKLGPFENLLLVTGENPAKAGVPYLAKALDIAKKYFSNLKIEVMP
LKAEEYAELKEHGLNGVICFQETYNKARYNIYHPRGMKSKFEWRVNGFDRMGQAGVHSIGMGVLIGLEEWRTDVTMMAYH
LRYLQKKYWKTKYSVNFPRMRPAENGGFQPNVIMNDRELAQLTFAMRIFDHDVDISYSTREPAQIRDNMAGLGVTTMSAE
SKTEPGGYYTYPQALEQFHVSDERTAVEVEHALKSLGREPVWKDWDVSFDKFTPIR
>Mature_376_residues
MFSEELEKIDWEETTKTIYSKTESDVLRALGKEHCDVDDFMALISPAAAKYLEPMAQLSKKYTEERFGKTISMFIPLYIT
NSCTNSCVYCGFHISNPMARTILTPEQIEDEYKAIKKLGPFENLLLVTGENPAKAGVPYLAKALDIAKKYFSNLKIEVMP
LKAEEYAELKEHGLNGVICFQETYNKARYNIYHPRGMKSKFEWRVNGFDRMGQAGVHSIGMGVLIGLEEWRTDVTMMAYH
LRYLQKKYWKTKYSVNFPRMRPAENGGFQPNVIMNDRELAQLTFAMRIFDHDVDISYSTREPAQIRDNMAGLGVTTMSAE
SKTEPGGYYTYPQALEQFHVSDERTAVEVEHALKSLGREPVWKDWDVSFDKFTPIR

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=366, Percent_Identity=46.448087431694, Blast_Score=349, Evalue=2e-97,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 43250; Mature: 43250

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSEELEKIDWEETTKTIYSKTESDVLRALGKEHCDVDDFMALISPAAAKYLEPMAQLSK
CCCHHHHHCCHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KYTEERFGKTISMFIPLYITNSCTNSCVYCGFHISNPMARTILTPEQIEDEYKAIKKLGP
HHHHHHHCCEEEEEEEEEEECCCCCCEEEEEEECCCCHHHHCCCHHHHHHHHHHHHHCCC
FENLLLVTGENPAKAGVPYLAKALDIAKKYFSNLKIEVMPLKAEEYAELKEHGLNGVICF
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCCEEEEE
QETYNKARYNIYHPRGMKSKFEWRVNGFDRMGQAGVHSIGMGVLIGLEEWRTDVTMMAYH
HHHCCCCCEEEECCCCCCCCEEEEECCHHHHCHHHHHHHCHHHEEEHHHHHHHHHHHHHH
LRYLQKKYWKTKYSVNFPRMRPAENGGFQPNVIMNDRELAQLTFAMRIFDHDVDISYSTR
HHHHHHHHHCCCEECCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCCEECCC
EPAQIRDNMAGLGVTTMSAESKTEPGGYYTYPQALEQFHVSDERTAVEVEHALKSLGREP
CCHHHHHCCCCCCEEEECCCCCCCCCCEECCHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
VWKDWDVSFDKFTPIR
CCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFSEELEKIDWEETTKTIYSKTESDVLRALGKEHCDVDDFMALISPAAAKYLEPMAQLSK
CCCHHHHHCCHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KYTEERFGKTISMFIPLYITNSCTNSCVYCGFHISNPMARTILTPEQIEDEYKAIKKLGP
HHHHHHHCCEEEEEEEEEEECCCCCCEEEEEEECCCCHHHHCCCHHHHHHHHHHHHHCCC
FENLLLVTGENPAKAGVPYLAKALDIAKKYFSNLKIEVMPLKAEEYAELKEHGLNGVICF
CCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHCCCCEEEEE
QETYNKARYNIYHPRGMKSKFEWRVNGFDRMGQAGVHSIGMGVLIGLEEWRTDVTMMAYH
HHHCCCCCEEEECCCCCCCCEEEEECCHHHHCHHHHHHHCHHHEEEHHHHHHHHHHHHHH
LRYLQKKYWKTKYSVNFPRMRPAENGGFQPNVIMNDRELAQLTFAMRIFDHDVDISYSTR
HHHHHHHHHCCCEECCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHCCCCCEECCC
EPAQIRDNMAGLGVTTMSAESKTEPGGYYTYPQALEQFHVSDERTAVEVEHALKSLGREP
CCHHHHHCCCCCCEEEECCCCCCCCCCEECCHHHHHHHCCCCCHHHHHHHHHHHHCCCCC
VWKDWDVSFDKFTPIR
CCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]