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The map label for this gene is glnA [H]

Identifier: 150006032

GI number: 150006032

Start: 4387771

End: 4389273

Strand: Direct

Name: glnA [H]

Synonym: BVU_3530

Alternate gene names: 150006032

Gene position: 4387771-4389273 (Clockwise)

Preceding gene: 150006030

Following gene: 150006035

Centisome position: 84.98

GC content: 43.11

Gene sequence:

>1503_bases
ATGAATAAAGAATTAATAATGAACCCCAACCAATTGGTGGCATTTCTAGAAAAACCTTGTGCAGAGTTTACCAAAGAAGA
CATAAAACGATACATTCAACAGAATGGCATCCGTATGGTCAACTTTATGTATCCCGCCGGAGACGGACGACTGAAGACAT
TGAATTTCGTTATCAACAACCAAGCATATCTAGATGCTATCTTGACTTGTGGAGAACGTGTGGACGGTTCCAGCCTGTTT
CCATTCATTGAGGCGGGCAGCAGTGATCTGTATGTAATTCCCCGATTCCGGACGGCTTTTTTGGACCCGTTGGCCGAGAT
ACCTACACTTTCCATGCTTTGCTCTTTCTTTAACAAGGATGGAGAGCCATTGGAAAGTTCCCCCGAACATACCTTACACA
AAGCATGCAAGGCATTCAATGAAGTAACCGGCATGGAATTTCAGGCTATGGGGGAATTAGAGTACTATGTAATTGCTCCG
GATACAAATATGTTCGAGGCTACAGATCAGAAAGGCTATCATGAATCAGCACCATATGCCAAGTTCAATGATTTCCGTAC
CCAGTGCATGGCCTACATTGCACAAGCAGGCGGACAAATAAAATATGGACATTCCGAAGTAGGTAATTTTACCTTGAATG
GCATGATATATGAACAGAATGAAATAGAATTCCTGCCTGTCAATGCCGAAGATGCGGCAGACCAATTGATGATCGCCAAA
TGGATTATACGTAATCTGGCCTATAAACATGGTTATAACATTACTTTCGCACCTAAAATAACTACAGGAAAAGCTGGTTC
CGGATTGCATATCCACATGCGTATCATGAAAGATGGACAAAACCAGATGTTGAAAGAAGGTGTTTTATCAGAAACAGCAC
GCAAAGCTATTGCTGGCATGATGGAACTTGCTCCATCCATCACCGCATTCGGTAATACCAATCCTACTTCTTATTTCCGC
CTGGTTCCCCATCAGGAAGCGCCAACTAATATTTGCTGGGGTGACCGCAACCGTTCAGTATTGGTACGCGTACCTCTAGG
ATGGGCAGCTAAAACAGACATGTGCATGACTGCCAATCCACTGGAAACAGAAAGCCATTATGATACCACACAAAAACAGA
CAGTGGAAATGCGGTCACCGGACGGTTCGGCCGATCTCTACCAACTGATAGCAGGATTAGCTGTAGCCTGTCGCCATGGA
TTTGAAATAGAAAATGCACTGGACATTGCAGAAAAAACTTACGTAAACGTCAATATCCATCAGAAAGAAAATGCAGACAA
ACTGAAGGCATTAGCCCAGCTTCCCGATAGCTGTGCAGCTTCCGCAACCTGTTTGCAACAACAGCGCGAAATATTCCAAA
AACATAATGTGTTCAGCCCCACTATGATTGATGGAATCATCAGCAAATTAACCAGTTATAACGATCTGACCTTACGTAAC
GACTTAAAAGATAATCCGGAAGGGATGTTGGCATTGGTCAATAAATATTTCCATTGCGGATAA

Upstream 100 bases:

>100_bases
GAAAGAAAATATCTATGAACTTTTCATAGGATTTTCAGAAAACTTTTTGTTATCTTTGGAAAGTTAACCAAATAACAAAC
AAAAAACAACCAATTTTGCT

Downstream 100 bases:

>100_bases
TTTGACTAAAACAGCGGGAGGAGGGTATATCAATATTTTGATTTGACCTTCTTCCGTTTTCTAACATTTACACCATGATA
AATAAAAAAGATCAATGTAC

Product: glutamine synthetase I

Products: NA

Alternate protein names: Glutamate--ammonia ligase I; Glutamine synthetase I; GSI [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MNKELIMNPNQLVAFLEKPCAEFTKEDIKRYIQQNGIRMVNFMYPAGDGRLKTLNFVINNQAYLDAILTCGERVDGSSLF
PFIEAGSSDLYVIPRFRTAFLDPLAEIPTLSMLCSFFNKDGEPLESSPEHTLHKACKAFNEVTGMEFQAMGELEYYVIAP
DTNMFEATDQKGYHESAPYAKFNDFRTQCMAYIAQAGGQIKYGHSEVGNFTLNGMIYEQNEIEFLPVNAEDAADQLMIAK
WIIRNLAYKHGYNITFAPKITTGKAGSGLHIHMRIMKDGQNQMLKEGVLSETARKAIAGMMELAPSITAFGNTNPTSYFR
LVPHQEAPTNICWGDRNRSVLVRVPLGWAAKTDMCMTANPLETESHYDTTQKQTVEMRSPDGSADLYQLIAGLAVACRHG
FEIENALDIAEKTYVNVNIHQKENADKLKALAQLPDSCAASATCLQQQREIFQKHNVFSPTMIDGIISKLTSYNDLTLRN
DLKDNPEGMLALVNKYFHCG

Sequences:

>Translated_500_residues
MNKELIMNPNQLVAFLEKPCAEFTKEDIKRYIQQNGIRMVNFMYPAGDGRLKTLNFVINNQAYLDAILTCGERVDGSSLF
PFIEAGSSDLYVIPRFRTAFLDPLAEIPTLSMLCSFFNKDGEPLESSPEHTLHKACKAFNEVTGMEFQAMGELEYYVIAP
DTNMFEATDQKGYHESAPYAKFNDFRTQCMAYIAQAGGQIKYGHSEVGNFTLNGMIYEQNEIEFLPVNAEDAADQLMIAK
WIIRNLAYKHGYNITFAPKITTGKAGSGLHIHMRIMKDGQNQMLKEGVLSETARKAIAGMMELAPSITAFGNTNPTSYFR
LVPHQEAPTNICWGDRNRSVLVRVPLGWAAKTDMCMTANPLETESHYDTTQKQTVEMRSPDGSADLYQLIAGLAVACRHG
FEIENALDIAEKTYVNVNIHQKENADKLKALAQLPDSCAASATCLQQQREIFQKHNVFSPTMIDGIISKLTSYNDLTLRN
DLKDNPEGMLALVNKYFHCG
>Mature_500_residues
MNKELIMNPNQLVAFLEKPCAEFTKEDIKRYIQQNGIRMVNFMYPAGDGRLKTLNFVINNQAYLDAILTCGERVDGSSLF
PFIEAGSSDLYVIPRFRTAFLDPLAEIPTLSMLCSFFNKDGEPLESSPEHTLHKACKAFNEVTGMEFQAMGELEYYVIAP
DTNMFEATDQKGYHESAPYAKFNDFRTQCMAYIAQAGGQIKYGHSEVGNFTLNGMIYEQNEIEFLPVNAEDAADQLMIAK
WIIRNLAYKHGYNITFAPKITTGKAGSGLHIHMRIMKDGQNQMLKEGVLSETARKAIAGMMELAPSITAFGNTNPTSYFR
LVPHQEAPTNICWGDRNRSVLVRVPLGWAAKTDMCMTANPLETESHYDTTQKQTVEMRSPDGSADLYQLIAGLAVACRHG
FEIENALDIAEKTYVNVNIHQKENADKLKALAQLPDSCAASATCLQQQREIFQKHNVFSPTMIDGIISKLTSYNDLTLRN
DLKDNPEGMLALVNKYFHCG

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=431, Percent_Identity=26.2180974477958, Blast_Score=116, Evalue=4e-27,
Organism=Escherichia coli, GI87081870, Length=348, Percent_Identity=26.1494252873563, Blast_Score=92, Evalue=8e-20,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 55998; Mature: 55998

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKELIMNPNQLVAFLEKPCAEFTKEDIKRYIQQNGIRMVNFMYPAGDGRLKTLNFVINN
CCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEEEECC
QAYLDAILTCGERVDGSSLFPFIEAGSSDLYVIPRFRTAFLDPLAEIPTLSMLCSFFNKD
HHHHHHHHHCCCCCCCCCCCHHEECCCCCEEEECCHHHHHHHHHHHCCHHHHHHHHHCCC
GEPLESSPEHTLHKACKAFNEVTGMEFQAMGELEYYVIAPDTNMFEATDQKGYHESAPYA
CCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCC
KFNDFRTQCMAYIAQAGGQIKYGHSEVGNFTLNGMIYEQNEIEFLPVNAEDAADQLMIAK
HHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEEEEECCCEEEEECCCHHHHHHHHHHH
WIIRNLAYKHGYNITFAPKITTGKAGSGLHIHMRIMKDGQNQMLKEGVLSETARKAIAGM
HHHHHHHHHCCCCEEECCEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
MELAPSITAFGNTNPTSYFRLVPHQEAPTNICWGDRNRSVLVRVPLGWAAKTDMCMTANP
HHHCCCCEECCCCCCCCEEEEECCCCCCCCCEECCCCCEEEEEECCCCCCCCCCEEECCC
LETESHYDTTQKQTVEMRSPDGSADLYQLIAGLAVACRHGFEIENALDIAEKTYVNVNIH
CCCCCCCCCCHHHEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEEEEE
QKENADKLKALAQLPDSCAASATCLQQQREIFQKHNVFSPTMIDGIISKLTSYNDLTLRN
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEECC
DLKDNPEGMLALVNKYFHCG
CCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNKELIMNPNQLVAFLEKPCAEFTKEDIKRYIQQNGIRMVNFMYPAGDGRLKTLNFVINN
CCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEEEECC
QAYLDAILTCGERVDGSSLFPFIEAGSSDLYVIPRFRTAFLDPLAEIPTLSMLCSFFNKD
HHHHHHHHHCCCCCCCCCCCHHEECCCCCEEEECCHHHHHHHHHHHCCHHHHHHHHHCCC
GEPLESSPEHTLHKACKAFNEVTGMEFQAMGELEYYVIAPDTNMFEATDQKGYHESAPYA
CCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCEEEEEECCCCCCCCCCCCCCCCCCCCCC
KFNDFRTQCMAYIAQAGGQIKYGHSEVGNFTLNGMIYEQNEIEFLPVNAEDAADQLMIAK
HHHHHHHHHHHHHHHCCCEEEECCCCCCCEEEEEEEEECCCEEEEECCCHHHHHHHHHHH
WIIRNLAYKHGYNITFAPKITTGKAGSGLHIHMRIMKDGQNQMLKEGVLSETARKAIAGM
HHHHHHHHHCCCCEEECCEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
MELAPSITAFGNTNPTSYFRLVPHQEAPTNICWGDRNRSVLVRVPLGWAAKTDMCMTANP
HHHCCCCEECCCCCCCCEEEEECCCCCCCCCEECCCCCEEEEEECCCCCCCCCCEEECCC
LETESHYDTTQKQTVEMRSPDGSADLYQLIAGLAVACRHGFEIENALDIAEKTYVNVNIH
CCCCCCCCCCHHHEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEEEEEEEE
QKENADKLKALAQLPDSCAASATCLQQQREIFQKHNVFSPTMIDGIISKLTSYNDLTLRN
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEECC
DLKDNPEGMLALVNKYFHCG
CCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8099074 [H]