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The map label for this gene is pyrB [H]

Identifier: 150005841

GI number: 150005841

Start: 4172491

End: 4173432

Strand: Direct

Name: pyrB [H]

Synonym: BVU_3337

Alternate gene names: 150005841

Gene position: 4172491-4173432 (Clockwise)

Preceding gene: 150005838

Following gene: 150005842

Centisome position: 80.81

GC content: 44.37

Gene sequence:

>942_bases
ATGGAAAATAGAAGTTTAGTTACTATTGCCGAACACTCGAAAGAGAAAATCCTCTACCTTTTGGAGATGGCAAAAGAGTT
TGAAAAGAAGCCTAACCGTAAAATCTTGGACGGCAAAGTTGTTGCCACCCTTTTCTTTGAGCCCTCCACCCGTACACGAC
TCAGTTTTGAAACCGCAGCAAACAGACTGGGAGCCCGCGTTATCGGATTTACCGATCCCAAAGTAACCAGTTCTTCAAAA
GGAGAAACATTGAAAGATACCATCATGATGGTAAGCAATTACGCCGACATCATCGTAATGCGCCATTTTCTGGAAGGTGC
CGCAAGATATGCCAGCGAAGTCGCCCCGGTGCCCATTGTCAATGCCGGAGACGGAGCCAACCAACATCCGTCACAAACCA
TGCTAGACCTCTACTCCATATACAAAACACAGGGAACGCTTGAAAATTTAAACATCTATCTAGTAGGCGACTTGAAATAC
GGACGAACTGTTCATTCTTTGCTGATGGCTATGCGTCATTTCAACCCCACTTTCCATTTCATCGCTCCGGAAGAGTTGAA
AATGCCTGAAGAATATAAAATCTACTGCAAGGAGCACCATATTAAATACAAAGAGTACACAGACTTTAACGAAGAAACCA
TTGCTGACGCCGACATTCTTTACATGACCCGTGTACAACGCGAACGTTTCACCGACCTGATGGAATATGAACGTGTGAAA
GATGTATATATATTAAGGAATAAGATGCTGGAGCATACCCGCCCCAATCTGCGTATCCTGCACCCTCTGCCCCGCGTCAA
CGAAATAGCTTACGATGTGGATGAGAATCCGAAAGCTTACTATTTCCAGCAGGCACAAAACGGACTTTATGCGCGCCAGG
CCATCCTTTGTGATGTATTGGGCATAACCTTGAATGATGTTATAGAAGATGCAAAAAAGTAA

Upstream 100 bases:

>100_bases
CTAACAAAGTTCAACGAAATATCTTTTAATTCTTCGCTCATTTATTTTTTTTTCATACCTTTACACTCCGTATAAATTGT
AATTATAAATACAAGCCAAT

Downstream 100 bases:

>100_bases
AAACAAAAATGAAGATGAGCGATAATAAACAAGCATTGCAGGTAGCCGCTTTGAAAAACGGCACTGTTATAGACCATATA
CCTTCCGATAAATTGTTTAC

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MENRSLVTIAEHSKEKILYLLEMAKEFEKKPNRKILDGKVVATLFFEPSTRTRLSFETAANRLGARVIGFTDPKVTSSSK
GETLKDTIMMVSNYADIIVMRHFLEGAARYASEVAPVPIVNAGDGANQHPSQTMLDLYSIYKTQGTLENLNIYLVGDLKY
GRTVHSLLMAMRHFNPTFHFIAPEELKMPEEYKIYCKEHHIKYKEYTDFNEETIADADILYMTRVQRERFTDLMEYERVK
DVYILRNKMLEHTRPNLRILHPLPRVNEIAYDVDENPKAYYFQQAQNGLYARQAILCDVLGITLNDVIEDAKK

Sequences:

>Translated_313_residues
MENRSLVTIAEHSKEKILYLLEMAKEFEKKPNRKILDGKVVATLFFEPSTRTRLSFETAANRLGARVIGFTDPKVTSSSK
GETLKDTIMMVSNYADIIVMRHFLEGAARYASEVAPVPIVNAGDGANQHPSQTMLDLYSIYKTQGTLENLNIYLVGDLKY
GRTVHSLLMAMRHFNPTFHFIAPEELKMPEEYKIYCKEHHIKYKEYTDFNEETIADADILYMTRVQRERFTDLMEYERVK
DVYILRNKMLEHTRPNLRILHPLPRVNEIAYDVDENPKAYYFQQAQNGLYARQAILCDVLGITLNDVIEDAKK
>Mature_313_residues
MENRSLVTIAEHSKEKILYLLEMAKEFEKKPNRKILDGKVVATLFFEPSTRTRLSFETAANRLGARVIGFTDPKVTSSSK
GETLKDTIMMVSNYADIIVMRHFLEGAARYASEVAPVPIVNAGDGANQHPSQTMLDLYSIYKTQGTLENLNIYLVGDLKY
GRTVHSLLMAMRHFNPTFHFIAPEELKMPEEYKIYCKEHHIKYKEYTDFNEETIADADILYMTRVQRERFTDLMEYERVK
DVYILRNKMLEHTRPNLRILHPLPRVNEIAYDVDENPKAYYFQQAQNGLYARQAILCDVLGITLNDVIEDAKK

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=303, Percent_Identity=38.6138613861386, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI38788445, Length=313, Percent_Identity=23.6421725239617, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI2367364, Length=300, Percent_Identity=51.6666666666667, Blast_Score=307, Evalue=7e-85,
Organism=Escherichia coli, GI1786469, Length=318, Percent_Identity=25.1572327044025, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790703, Length=320, Percent_Identity=24.375, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193204318, Length=320, Percent_Identity=38.75, Blast_Score=214, Evalue=4e-56,
Organism=Saccharomyces cerevisiae, GI6322331, Length=306, Percent_Identity=40.5228758169935, Blast_Score=251, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6322373, Length=316, Percent_Identity=24.0506329113924, Blast_Score=62, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24642586, Length=272, Percent_Identity=41.9117647058824, Blast_Score=233, Evalue=2e-61,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 36258; Mature: 36258

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENRSLVTIAEHSKEKILYLLEMAKEFEKKPNRKILDGKVVATLFFEPSTRTRLSFETAA
CCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEECCEEEEEEEECCCCCCEEEHHHHH
NRLGARVIGFTDPKVTSSSKGETLKDTIMMVSNYADIIVMRHFLEGAARYASEVAPVPIV
HHHCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
NAGDGANQHPSQTMLDLYSIYKTQGTLENLNIYLVGDLKYGRTVHSLLMAMRHFNPTFHF
ECCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCEEEE
IAPEELKMPEEYKIYCKEHHIKYKEYTDFNEETIADADILYMTRVQRERFTDLMEYERVK
ECHHHHCCCHHHHHHHHHCCEEEHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
DVYILRNKMLEHTRPNLRILHPLPRVNEIAYDVDENPKAYYFQQAQNGLYARQAILCDVL
HHHHHHHHHHHHCCCCEEEECCCCCHHHHEECCCCCCCEEEHHHHHCCHHHHHHHHHHHH
GITLNDVIEDAKK
HHHHHHHHHHHCC
>Mature Secondary Structure
MENRSLVTIAEHSKEKILYLLEMAKEFEKKPNRKILDGKVVATLFFEPSTRTRLSFETAA
CCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCEEECCEEEEEEEECCCCCCEEEHHHHH
NRLGARVIGFTDPKVTSSSKGETLKDTIMMVSNYADIIVMRHFLEGAARYASEVAPVPIV
HHHCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
NAGDGANQHPSQTMLDLYSIYKTQGTLENLNIYLVGDLKYGRTVHSLLMAMRHFNPTFHF
ECCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCEEEE
IAPEELKMPEEYKIYCKEHHIKYKEYTDFNEETIADADILYMTRVQRERFTDLMEYERVK
ECHHHHCCCHHHHHHHHHCCEEEHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
DVYILRNKMLEHTRPNLRILHPLPRVNEIAYDVDENPKAYYFQQAQNGLYARQAILCDVL
HHHHHHHHHHHHCCCCEEEECCCCCHHHHEECCCCCCCEEEHHHHHCCHHHHHHHHHHHH
GITLNDVIEDAKK
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12663928 [H]