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The map label for this gene is trmD

Identifier: 150005811

GI number: 150005811

Start: 4144073

End: 4144747

Strand: Direct

Name: trmD

Synonym: BVU_3307

Alternate gene names: 150005811

Gene position: 4144073-4144747 (Clockwise)

Preceding gene: 150005808

Following gene: 150005812

Centisome position: 80.26

GC content: 42.67

Gene sequence:

>675_bases
ATGAGAATAGACATCATTACCGTTTTACCCGAGATGCTGGAAGGCTTCTTTAACTGCTCTATCATGAGCCGTGCGCAGAA
AAAAGGAATTGCAGAAATACATATACATAACCTGCGTGATTATGCATACGACCGTTATCGCAAAGTAGACGATTATCCCT
TCGGTGGATTTGCCGGTATGGTGATGAAGATTGAACCGATAGACCGATGCATCTCAGCTTTGAAAGCAGAACGCGAGTAT
GACGAAATAATATTTACATCTCCAGATGGAGAGCAGTTTAACCAGCCGATGGCCAACTCACTTTCACTCGCAAAGAATTT
AATTATTCTATGTGGCCATTTTAAGGGTATAGACTATAGAATACGTGAACATTTCATTACGAAAGAAATCAGTATCGGTG
ATTATGTTTTGACAGGTGGAGAGTTGGCTGCCGCCGTTATTGCCGATGCCGTAGTTCGTATTATTCCCGGGGTCATTTCT
GACGAACAATCGGCATTGTCCGATTCTTTTCAAGACAATCTGTTAGCTGCTCCTGTATACACTCGTCCTGCAGAATACAA
AGGATGGCAAGTTCCCGATGTGTTGTTATCCGGACACGAGGCAAAGATTAAAGAATGGGAACTTCAGCAGTCATTAGAAA
GGACACAGCGGCTAAGACCCGATTTGTTGAAATAA

Upstream 100 bases:

>100_bases
ATATATAAAAGGTAGTTTTCCCGCAAAAATAGCTATTTTTTGAATGAAAAACACCTTCCGACAAAGAGGAATTTGCTACT
TTTGCAAAAAATAACCGAAT

Downstream 100 bases:

>100_bases
AAAATATATATAAATAATAAAGAGGTAAAAGCCATGTTCGTCATAGGGCTTTTACCTCTTTTCTTTTGCTTATATCATAT
CCGAAAAGATGTTCTCTCCT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MRIDIITVLPEMLEGFFNCSIMSRAQKKGIAEIHIHNLRDYAYDRYRKVDDYPFGGFAGMVMKIEPIDRCISALKAEREY
DEIIFTSPDGEQFNQPMANSLSLAKNLIILCGHFKGIDYRIREHFITKEISIGDYVLTGGELAAAVIADAVVRIIPGVIS
DEQSALSDSFQDNLLAAPVYTRPAEYKGWQVPDVLLSGHEAKIKEWELQQSLERTQRLRPDLLK

Sequences:

>Translated_224_residues
MRIDIITVLPEMLEGFFNCSIMSRAQKKGIAEIHIHNLRDYAYDRYRKVDDYPFGGFAGMVMKIEPIDRCISALKAEREY
DEIIFTSPDGEQFNQPMANSLSLAKNLIILCGHFKGIDYRIREHFITKEISIGDYVLTGGELAAAVIADAVVRIIPGVIS
DEQSALSDSFQDNLLAAPVYTRPAEYKGWQVPDVLLSGHEAKIKEWELQQSLERTQRLRPDLLK
>Mature_224_residues
MRIDIITVLPEMLEGFFNCSIMSRAQKKGIAEIHIHNLRDYAYDRYRKVDDYPFGGFAGMVMKIEPIDRCISALKAEREY
DEIIFTSPDGEQFNQPMANSLSLAKNLIILCGHFKGIDYRIREHFITKEISIGDYVLTGGELAAAVIADAVVRIIPGVIS
DEQSALSDSFQDNLLAAPVYTRPAEYKGWQVPDVLLSGHEAKIKEWELQQSLERTQRLRPDLLK

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=225, Percent_Identity=44.4444444444444, Blast_Score=192, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_BACV8 (A6L5G9)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001300555.1
- ProteinModelPortal:   A6L5G9
- SMR:   A6L5G9
- STRING:   A6L5G9
- GeneID:   5304268
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_3307
- NMPDR:   fig|435590.6.peg.3083
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 25423; Mature: 25423

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: BINDING 112-112

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDIITVLPEMLEGFFNCSIMSRAQKKGIAEIHIHNLRDYAYDRYRKVDDYPFGGFAGM
CEEEHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCCCCCCHHHH
VMKIEPIDRCISALKAEREYDEIIFTSPDGEQFNQPMANSLSLAKNLIILCGHFKGIDYR
EEEECHHHHHHHHHHHHCCCCCEEEECCCCHHHCCHHHHHHHHHHHHHEEEECCCCCHHH
IREHFITKEISIGDYVLTGGELAAAVIADAVVRIIPGVISDEQSALSDSFQDNLLAAPVY
HHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEECCCC
TRPAEYKGWQVPDVLLSGHEAKIKEWELQQSLERTQRLRPDLLK
CCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRIDIITVLPEMLEGFFNCSIMSRAQKKGIAEIHIHNLRDYAYDRYRKVDDYPFGGFAGM
CEEEHHHHHHHHHHHHHCHHHHHHHHHCCCCEEEHHHHHHHHHHHHHCCCCCCCCCHHHH
VMKIEPIDRCISALKAEREYDEIIFTSPDGEQFNQPMANSLSLAKNLIILCGHFKGIDYR
EEEECHHHHHHHHHHHHCCCCCEEEECCCCHHHCCHHHHHHHHHHHHHEEEECCCCCHHH
IREHFITKEISIGDYVLTGGELAAAVIADAVVRIIPGVISDEQSALSDSFQDNLLAAPVY
HHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCEEECCCC
TRPAEYKGWQVPDVLLSGHEAKIKEWELQQSLERTQRLRPDLLK
CCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA