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The map label for this gene is rimO

Identifier: 150005789

GI number: 150005789

Start: 4112613

End: 4113911

Strand: Direct

Name: rimO

Synonym: BVU_3282

Alternate gene names: 150005789

Gene position: 4112613-4113911 (Clockwise)

Preceding gene: 150005788

Following gene: 150005790

Centisome position: 79.65

GC content: 40.11

Gene sequence:

>1299_bases
ATGAAACGTAACACAATAGATATCATCACCTTAGGGTGTTCCAAAAACTTAGTAGACTCAGAAAAGCTGATGCGTCAGCT
GGAAGCAAACGGTTATAAAGTAACTCACGATTCCGACAAACCGCAAGGAGAAATCGCAGTTATTAATACATGTGGTTTCA
TCGGTGATGCCAAAGAGGAATCGATCAATATGATATTGGAATTTTGTCAGGCCAAAGAAGAAGGGAAGCTCAAAAAACTA
TATGTGATGGGCTGCCTTTCAGAAAGATATCTAAAAGAATTGGCATTGGAAATTCCTCAAGTAGATAAATTCTATGGTAA
GTTTAACTGGAATGAGTTGCTGGCAGATTTAGGCAAAGCATACAAGTCGGAATTTGCCATTGAACGGACATTAACCACAC
CCCATCACTACGCTTATCTGAAAATATCAGAAGGCTGTGACCGTAAATGCTCTTATTGCGCCATTCCTATTATTACAGGA
CGCCACATTTCACGGCCGATGGAGGAGATTATTGATGAAGTGAAACTACTTGTATCCGAAGGAGTAAAAGAGTTCCAAAT
TATAGCCCAAGAATTGACTTACTACGGCGTAGATTTATATAAAAGTCAGAAATTACCCGAACTCATCGAAAGAATCGCCA
ATGTTCCCGGTGTAGAATGGATACGTTTACACTATGCATATCCGGCCCATTTTCCGGAAGAATTATTCCGTGTTATGCGC
GAACACGATAATGTATGCAAATATATGGATATTGCCTTGCAACACATTAGTGACAATATGCTGAATAAAATGCGCCGGCA
TGTAAGTAAAGCGGAAACCTACGAATTAATCGAAAAATTCCGTCGGGAAGTACCCGGTATTCACTTACGTACAACATTAA
TGGTAGGACATCCCGGTGAGACTGAAGAAGATTTTGAAGAATTAAAAGAATTCGTCAAAAAAGTACGATTCGACCGGATG
GGAGCCTTCGCTTACTCGGAAGAAGAAGGAACATTCGCCGCTAAAGAATACGAAGATTCCATTTCTCATGAAGTGAAACA
ACAGCGTTTGGATGAATTAATGGCACTTCAGCAGGAAATAGCCGGCGAACTGAGCCAAACGAAAATAGGTAAAGAATTTA
AAGTCATTATAGACCGGAAGGAAGGAGATTACTACATAGGCAGAACCCAGTTTGACTCTCCCGAGGTAGATCCTGAGGTC
TTGATAAAAGCTGATGAGGAGTACTTAAAAATAGGTGAATTCTATAAAGTGAAAATTACAGCCGCTGATGATTTCGACCT
ATACGCCAGCATTTTATAG

Upstream 100 bases:

>100_bases
TCCCGTAAAATATATTGGATTGGGCGAAGGCATTGAAGACATGCAGGTATTCCGCAAAAAAGAATTTGTAGACTCACTAT
TTGGAGAGACTGAATAATAA

Downstream 100 bases:

>100_bases
ACCTTTGCCCTTATAAAGTTTGGATAATGTTTGTTTTTTTACTAATATAGCAACCAAATTAACACTTTAAGCTTTGAATA
ACAAAGAATTTATATCTGAA

Product: putative Fe-S oxidoreductase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MKRNTIDIITLGCSKNLVDSEKLMRQLEANGYKVTHDSDKPQGEIAVINTCGFIGDAKEESINMILEFCQAKEEGKLKKL
YVMGCLSERYLKELALEIPQVDKFYGKFNWNELLADLGKAYKSEFAIERTLTTPHHYAYLKISEGCDRKCSYCAIPIITG
RHISRPMEEIIDEVKLLVSEGVKEFQIIAQELTYYGVDLYKSQKLPELIERIANVPGVEWIRLHYAYPAHFPEELFRVMR
EHDNVCKYMDIALQHISDNMLNKMRRHVSKAETYELIEKFRREVPGIHLRTTLMVGHPGETEEDFEELKEFVKKVRFDRM
GAFAYSEEEGTFAAKEYEDSISHEVKQQRLDELMALQQEIAGELSQTKIGKEFKVIIDRKEGDYYIGRTQFDSPEVDPEV
LIKADEEYLKIGEFYKVKITAADDFDLYASIL

Sequences:

>Translated_432_residues
MKRNTIDIITLGCSKNLVDSEKLMRQLEANGYKVTHDSDKPQGEIAVINTCGFIGDAKEESINMILEFCQAKEEGKLKKL
YVMGCLSERYLKELALEIPQVDKFYGKFNWNELLADLGKAYKSEFAIERTLTTPHHYAYLKISEGCDRKCSYCAIPIITG
RHISRPMEEIIDEVKLLVSEGVKEFQIIAQELTYYGVDLYKSQKLPELIERIANVPGVEWIRLHYAYPAHFPEELFRVMR
EHDNVCKYMDIALQHISDNMLNKMRRHVSKAETYELIEKFRREVPGIHLRTTLMVGHPGETEEDFEELKEFVKKVRFDRM
GAFAYSEEEGTFAAKEYEDSISHEVKQQRLDELMALQQEIAGELSQTKIGKEFKVIIDRKEGDYYIGRTQFDSPEVDPEV
LIKADEEYLKIGEFYKVKITAADDFDLYASIL
>Mature_432_residues
MKRNTIDIITLGCSKNLVDSEKLMRQLEANGYKVTHDSDKPQGEIAVINTCGFIGDAKEESINMILEFCQAKEEGKLKKL
YVMGCLSERYLKELALEIPQVDKFYGKFNWNELLADLGKAYKSEFAIERTLTTPHHYAYLKISEGCDRKCSYCAIPIITG
RHISRPMEEIIDEVKLLVSEGVKEFQIIAQELTYYGVDLYKSQKLPELIERIANVPGVEWIRLHYAYPAHFPEELFRVMR
EHDNVCKYMDIALQHISDNMLNKMRRHVSKAETYELIEKFRREVPGIHLRTTLMVGHPGETEEDFEELKEFVKKVRFDRM
GAFAYSEEEGTFAAKEYEDSISHEVKQQRLDELMALQQEIAGELSQTKIGKEFKVIIDRKEGDYYIGRTQFDSPEVDPEV
LIKADEEYLKIGEFYKVKITAADDFDLYASIL

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=473, Percent_Identity=26.215644820296, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI28872782, Length=473, Percent_Identity=26.215644820296, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI93277076, Length=407, Percent_Identity=27.5184275184275, Blast_Score=130, Evalue=3e-30,
Organism=Escherichia coli, GI1787057, Length=445, Percent_Identity=37.3033707865169, Blast_Score=278, Evalue=7e-76,
Organism=Escherichia coli, GI1786882, Length=444, Percent_Identity=26.5765765765766, Blast_Score=155, Evalue=5e-39,
Organism=Caenorhabditis elegans, GI17553146, Length=473, Percent_Identity=24.3128964059197, Blast_Score=141, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI71996771, Length=293, Percent_Identity=27.6450511945392, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21356207, Length=463, Percent_Identity=27.2138228941685, Blast_Score=172, Evalue=5e-43,
Organism=Drosophila melanogaster, GI19922432, Length=445, Percent_Identity=25.6179775280899, Blast_Score=119, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_BACV8 (A6L5E7)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001300533.1
- ProteinModelPortal:   A6L5E7
- STRING:   A6L5E7
- GeneID:   5304243
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_3282
- NMPDR:   fig|435590.6.peg.3062
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- ProtClustDB:   CLSK822448
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 50102; Mature: 50102

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRNTIDIITLGCSKNLVDSEKLMRQLEANGYKVTHDSDKPQGEIAVINTCGFIGDAKEE
CCCCEEEEEEECCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCEEEEEECCCCCCCHHH
SINMILEFCQAKEEGKLKKLYVMGCLSERYLKELALEIPQVDKFYGKFNWNELLADLGKA
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHH
YKSEFAIERTLTTPHHYAYLKISEGCDRKCSYCAIPIITGRHISRPMEEIIDEVKLLVSE
HHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEEEECCCHHCCHHHHHHHHHHHHHHH
GVKEFQIIAQELTYYGVDLYKSQKLPELIERIANVPGVEWIRLHYAYPAHFPEELFRVMR
CHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHH
EHDNVCKYMDIALQHISDNMLNKMRRHVSKAETYELIEKFRREVPGIHLRTTLMVGHPGE
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCC
TEEDFEELKEFVKKVRFDRMGAFAYSEEEGTFAAKEYEDSISHEVKQQRLDELMALQQEI
CHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGELSQTKIGKEFKVIIDRKEGDYYIGRTQFDSPEVDPEVLIKADEEYLKIGEFYKVKIT
HHHHHHHHCCCEEEEEEECCCCCEEECCCCCCCCCCCHHHEEECCHHHHHCCCEEEEEEE
AADDFDLYASIL
ECCCCHHHHHCC
>Mature Secondary Structure
MKRNTIDIITLGCSKNLVDSEKLMRQLEANGYKVTHDSDKPQGEIAVINTCGFIGDAKEE
CCCCEEEEEEECCCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCEEEEEECCCCCCCHHH
SINMILEFCQAKEEGKLKKLYVMGCLSERYLKELALEIPQVDKFYGKFNWNELLADLGKA
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHH
YKSEFAIERTLTTPHHYAYLKISEGCDRKCSYCAIPIITGRHISRPMEEIIDEVKLLVSE
HHHHHHHHHHCCCCCCEEEEEECCCCCCCCCEEEEEEECCCHHCCHHHHHHHHHHHHHHH
GVKEFQIIAQELTYYGVDLYKSQKLPELIERIANVPGVEWIRLHYAYPAHFPEELFRVMR
CHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHH
EHDNVCKYMDIALQHISDNMLNKMRRHVSKAETYELIEKFRREVPGIHLRTTLMVGHPGE
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCC
TEEDFEELKEFVKKVRFDRMGAFAYSEEEGTFAAKEYEDSISHEVKQQRLDELMALQQEI
CHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGELSQTKIGKEFKVIIDRKEGDYYIGRTQFDSPEVDPEVLIKADEEYLKIGEFYKVKIT
HHHHHHHHCCCEEEEEEECCCCCEEECCCCCCCCCCCHHHEEECCHHHHHCCCEEEEEEE
AADDFDLYASIL
ECCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA