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The map label for this gene is ogt [H]

Identifier: 150005728

GI number: 150005728

Start: 4045719

End: 4046246

Strand: Direct

Name: ogt [H]

Synonym: BVU_3221

Alternate gene names: 150005728

Gene position: 4045719-4046246 (Clockwise)

Preceding gene: 150005727

Following gene: 150005734

Centisome position: 78.36

GC content: 43.94

Gene sequence:

>528_bases
ATGAAAGAGAAAAATACGATCAAGATAAAGAGTTATGAATCACCCTGCGGAGTGTTGCTGCTCGGTTCATTTGGCGGAAA
ACTCTGCCTGTGTGACTGGCAGGTGGAAAAACACTGCGATCGTGTGAATCAACGGTTGAAGCGGGTATTGTGTGCCGAAT
TTGAAATAAGTACGTCAGAAATAATCGAAGAATCAATAGAGCAACTCAACGAGTATTTTGCAGGACAACGCAAAGAATTT
AGTGTGCCGCTTCTTTTTGTGGGCACGGATTTTCAGAAAACAGTATGGAATGAACTGTTAAAGATACCGTTTGGCAGGAC
AATGTCTTATGGTGAAATGGCCAGGCATATCGGGATGCCCCAAGCAGTTCGTGCGGTAGCCAATGCTAATGGTGCAAATT
CCATATCAATCTTTGCACCTTGCCATCGTGTGATCGGTAGTGATCACTCGCTGACAGGATATGGAGGCGGACTGTCCGCT
AAAAGGTTTTTATTGGAATTGGAATCACAACCTCGGTTAATGTTATGA

Upstream 100 bases:

>100_bases
GCTGTTTCAGCTAATTCGCTACGCTTTGTCTTTTTGGTTTAAAGTAGTCCGATAAGCTGAAACAGCTTATAAACATATGA
AGGAAGCATATAAAAAGTGT

Downstream 100 bases:

>100_bases
GCAGACAAGAAACATTTCCAATGCTATAATAATAAATAAAAAGGAATGGGTAGTGAAAATGCCCATTCCTTTTTCTCATA
TCTTCTTAATGAATTACCAT

Product: methylated-DNA-protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MKEKNTIKIKSYESPCGVLLLGSFGGKLCLCDWQVEKHCDRVNQRLKRVLCAEFEISTSEIIEESIEQLNEYFAGQRKEF
SVPLLFVGTDFQKTVWNELLKIPFGRTMSYGEMARHIGMPQAVRAVANANGANSISIFAPCHRVIGSDHSLTGYGGGLSA
KRFLLELESQPRLML

Sequences:

>Translated_175_residues
MKEKNTIKIKSYESPCGVLLLGSFGGKLCLCDWQVEKHCDRVNQRLKRVLCAEFEISTSEIIEESIEQLNEYFAGQRKEF
SVPLLFVGTDFQKTVWNELLKIPFGRTMSYGEMARHIGMPQAVRAVANANGANSISIFAPCHRVIGSDHSLTGYGGGLSA
KRFLLELESQPRLML
>Mature_175_residues
MKEKNTIKIKSYESPCGVLLLGSFGGKLCLCDWQVEKHCDRVNQRLKRVLCAEFEISTSEIIEESIEQLNEYFAGQRKEF
SVPLLFVGTDFQKTVWNELLKIPFGRTMSYGEMARHIGMPQAVRAVANANGANSISIFAPCHRVIGSDHSLTGYGGGLSA
KRFLLELESQPRLML

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=122, Percent_Identity=36.0655737704918, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI1787596, Length=103, Percent_Identity=48.5436893203884, Blast_Score=102, Evalue=1e-23,
Organism=Escherichia coli, GI1788542, Length=109, Percent_Identity=44.0366972477064, Blast_Score=93, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=50, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=50, Blast_Score=80, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6320001, Length=96, Percent_Identity=43.75, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19617; Mature: 19617

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
6.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEKNTIKIKSYESPCGVLLLGSFGGKLCLCDWQVEKHCDRVNQRLKRVLCAEFEISTSE
CCCCCEEEEEECCCCCCEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHH
IIEESIEQLNEYFAGQRKEFSVPLLFVGTDFQKTVWNELLKIPFGRTMSYGEMARHIGMP
HHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCH
QAVRAVANANGANSISIFAPCHRVIGSDHSLTGYGGGLSAKRFLLELESQPRLML
HHHHHHHCCCCCCEEEEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKEKNTIKIKSYESPCGVLLLGSFGGKLCLCDWQVEKHCDRVNQRLKRVLCAEFEISTSE
CCCCCEEEEEECCCCCCEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCHHH
IIEESIEQLNEYFAGQRKEFSVPLLFVGTDFQKTVWNELLKIPFGRTMSYGEMARHIGMP
HHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCCHHHHHHHCCCH
QAVRAVANANGANSISIFAPCHRVIGSDHSLTGYGGGLSAKRFLLELESQPRLML
HHHHHHHCCCCCCEEEEHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]