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The map label for this gene is aspS

Identifier: 150005727

GI number: 150005727

Start: 4043781

End: 4045544

Strand: Direct

Name: aspS

Synonym: BVU_3220

Alternate gene names: 150005727

Gene position: 4043781-4045544 (Clockwise)

Preceding gene: 150005726

Following gene: 150005728

Centisome position: 78.32

GC content: 43.76

Gene sequence:

>1764_bases
ATGTTCAGAAGTCATACTTGCGGAGAGCTCAGACTTTCCGATGCAGGCAAAAGTGTAACGTTGGCAGGTTGGGTACAGCG
TGCACGTAAAATGGGAGGGATGACGTTTGTCGATCTTCGTGACCGTTACGGTATTACCCAATTGGTATTCAACGAAGCGG
TGAATGCCGAACTTTGTGAACGTGCCAATCACTTGGGGCGTGAATTTGTTATTCAAATAACCGGTGAAGTGAACGAACGT
TCAAATAAGAATATGAATATTCCCACCGGTGAAATTGAAATTATCGTATCGGTATTGAATGTACTTAATTCAGCGGTAAC
TCCTCCTTTTACGATAGAAGATAATAGTGACGGAGGTGATGATATCCGTATGAAATTCCGCTATTTGGATTTACGCCGTA
ATTGTGTGCGTAAAAATTTGGAACTTCGTCATAAAATGACAATGGAAGTGCGCCGATATTTGGACAGCAAAGGGTTTCTG
GAAGTGGAAACTCCTATGTTGGTAGGTTCTACTCCTGAAGGAGCCCGTGATTTTGTTGTTCCTTCACGTATGAACCCGGG
ACAGTTTTATGCACTGCCACAGTCTCCGCAAACACTGAAGCAATTGCTGATGGTTTCCGGCTTTGATCGTTACTTCCAGA
TTGTAAAGTGTTTCCGTGATGAAGATCTTCGTGCAGACCGTCAGCCTGAGTTTACTCAGATAGACTGTGAGATGAGTTTT
GTTGAACAAGAAGATATCATTTCCACTTTCGAAGGAATGGCAAAACATTTGTTCAAGGAGTTACGTGGAGTCGAGTTGAG
CGAACCGTTTCTGCGTATGACATGGGCCGATGCCATAAAATATTACGGTAGTGACAAACCGGATTTGCGTTTCGGCATGA
AGTTTGTGGAATTGATGGATATCATGAAAGGTCATGGCTTCTCTGTTTTTGATGATGCGGCATATATTGGTGGTATCTGT
GCGGAAGGCGCTGCAAGTTATACCCGTAAACAGTTAGACCAATTAACAGAATTTGTCAAGAAACCTCAGATCGGTGCGAA
AGGTATGGTTTACGCCCGTGTGGAAGCTGATGGCAATGTAAAATCCAGTGTAGACAAATTCTATTCACAGGAAGTACTTC
AGCAGATGAAGGAAGCTTTCAGTGCCAAGCCGGGTGACTTGATCCTGATTCTGAGTGGTCCTGATGCTATGAAGACCCGC
AAGCAGTTATGCGAACTTCGTCTGGAAGTGGGACGTCAGCTGGGACTGCGTGATAAGAATAAATTTGCTTGTCTGTGGGT
TGTTGATTTTCCGATGTTTGAGTGGAGTGAAGAAGAAGGCCGTCTGATGGCCATGCACCATCCGTTCACTCATCCGAAAG
AAGAAGATATTCCTTTGTTGGATACAGATCCGGCTGCTGTTCGTGCCGATGCTTACGATATGGTAGTAAACGGTGTAGAA
GTAGGAGGTGGTTCAATTCGTATTCATGATTCACAATTACAAGCCAAGATGTTTGAAATTTTGGGCTTTACTCCGGAAAG
AGCACAAGAACAATTCGGCTTCCTGATGAATGCATTCAAATACGGAGCACCTCCTCATGGTGGTTTGGCATACGGTCTTG
ATCGCTGGGTTTCTTTGTTTGCAGGACTTGATAGTATCCGTGACTGTATAGCATTCCCTAAAAATAATAGTGGACGTGAT
GTGATGTTGGATGCTCCCGCTGCTTTGGATCAATCACAATTGGATGAGTTGAATCTGGTTGTTGATATAAAAGAGGATAA
GTAA

Upstream 100 bases:

>100_bases
CGTTGGTATACAGCTACGGGACTTTTTTTCTTGTAGGCAGTTTTGTACAATGGAAAACATATCGTACCTTTGCCAAACAA
TTGATTAAATTTAAGAATAT

Downstream 100 bases:

>100_bases
GTTTTTTCCGCTTTTTATATAGGATATTGTATAAAAGGCCGATAACGGGACGATCCGAACAAAGCGTAGTGAATGCTGTT
TCAGCTAATTCGCTACGCTT

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MFRSHTCGELRLSDAGKSVTLAGWVQRARKMGGMTFVDLRDRYGITQLVFNEAVNAELCERANHLGREFVIQITGEVNER
SNKNMNIPTGEIEIIVSVLNVLNSAVTPPFTIEDNSDGGDDIRMKFRYLDLRRNCVRKNLELRHKMTMEVRRYLDSKGFL
EVETPMLVGSTPEGARDFVVPSRMNPGQFYALPQSPQTLKQLLMVSGFDRYFQIVKCFRDEDLRADRQPEFTQIDCEMSF
VEQEDIISTFEGMAKHLFKELRGVELSEPFLRMTWADAIKYYGSDKPDLRFGMKFVELMDIMKGHGFSVFDDAAYIGGIC
AEGAASYTRKQLDQLTEFVKKPQIGAKGMVYARVEADGNVKSSVDKFYSQEVLQQMKEAFSAKPGDLILILSGPDAMKTR
KQLCELRLEVGRQLGLRDKNKFACLWVVDFPMFEWSEEEGRLMAMHHPFTHPKEEDIPLLDTDPAAVRADAYDMVVNGVE
VGGGSIRIHDSQLQAKMFEILGFTPERAQEQFGFLMNAFKYGAPPHGGLAYGLDRWVSLFAGLDSIRDCIAFPKNNSGRD
VMLDAPAALDQSQLDELNLVVDIKEDK

Sequences:

>Translated_587_residues
MFRSHTCGELRLSDAGKSVTLAGWVQRARKMGGMTFVDLRDRYGITQLVFNEAVNAELCERANHLGREFVIQITGEVNER
SNKNMNIPTGEIEIIVSVLNVLNSAVTPPFTIEDNSDGGDDIRMKFRYLDLRRNCVRKNLELRHKMTMEVRRYLDSKGFL
EVETPMLVGSTPEGARDFVVPSRMNPGQFYALPQSPQTLKQLLMVSGFDRYFQIVKCFRDEDLRADRQPEFTQIDCEMSF
VEQEDIISTFEGMAKHLFKELRGVELSEPFLRMTWADAIKYYGSDKPDLRFGMKFVELMDIMKGHGFSVFDDAAYIGGIC
AEGAASYTRKQLDQLTEFVKKPQIGAKGMVYARVEADGNVKSSVDKFYSQEVLQQMKEAFSAKPGDLILILSGPDAMKTR
KQLCELRLEVGRQLGLRDKNKFACLWVVDFPMFEWSEEEGRLMAMHHPFTHPKEEDIPLLDTDPAAVRADAYDMVVNGVE
VGGGSIRIHDSQLQAKMFEILGFTPERAQEQFGFLMNAFKYGAPPHGGLAYGLDRWVSLFAGLDSIRDCIAFPKNNSGRD
VMLDAPAALDQSQLDELNLVVDIKEDK
>Mature_587_residues
MFRSHTCGELRLSDAGKSVTLAGWVQRARKMGGMTFVDLRDRYGITQLVFNEAVNAELCERANHLGREFVIQITGEVNER
SNKNMNIPTGEIEIIVSVLNVLNSAVTPPFTIEDNSDGGDDIRMKFRYLDLRRNCVRKNLELRHKMTMEVRRYLDSKGFL
EVETPMLVGSTPEGARDFVVPSRMNPGQFYALPQSPQTLKQLLMVSGFDRYFQIVKCFRDEDLRADRQPEFTQIDCEMSF
VEQEDIISTFEGMAKHLFKELRGVELSEPFLRMTWADAIKYYGSDKPDLRFGMKFVELMDIMKGHGFSVFDDAAYIGGIC
AEGAASYTRKQLDQLTEFVKKPQIGAKGMVYARVEADGNVKSSVDKFYSQEVLQQMKEAFSAKPGDLILILSGPDAMKTR
KQLCELRLEVGRQLGLRDKNKFACLWVVDFPMFEWSEEEGRLMAMHHPFTHPKEEDIPLLDTDPAAVRADAYDMVVNGVE
VGGGSIRIHDSQLQAKMFEILGFTPERAQEQFGFLMNAFKYGAPPHGGLAYGLDRWVSLFAGLDSIRDCIAFPKNNSGRD
VMLDAPAALDQSQLDELNLVVDIKEDK

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI40789249, Length=606, Percent_Identity=40.7590759075908, Blast_Score=420, Evalue=1e-117,
Organism=Homo sapiens, GI194272210, Length=253, Percent_Identity=28.0632411067194, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI5031815, Length=254, Percent_Identity=28.3464566929134, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI45439306, Length=306, Percent_Identity=23.8562091503268, Blast_Score=81, Evalue=3e-15,
Organism=Escherichia coli, GI1788173, Length=591, Percent_Identity=48.7309644670051, Blast_Score=545, Evalue=1e-156,
Organism=Escherichia coli, GI1789256, Length=304, Percent_Identity=28.6184210526316, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1790571, Length=304, Percent_Identity=28.2894736842105, Blast_Score=98, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI32566633, Length=586, Percent_Identity=38.2252559726962, Blast_Score=353, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI17551876, Length=334, Percent_Identity=23.0538922155689, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71994340, Length=298, Percent_Identity=24.8322147651007, Blast_Score=86, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17535925, Length=298, Percent_Identity=24.8322147651007, Blast_Score=86, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17535927, Length=298, Percent_Identity=24.8322147651007, Blast_Score=86, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI71984122, Length=309, Percent_Identity=24.9190938511327, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6325153, Length=636, Percent_Identity=32.8616352201258, Blast_Score=294, Evalue=2e-80,
Organism=Saccharomyces cerevisiae, GI6323011, Length=268, Percent_Identity=26.865671641791, Blast_Score=81, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6321807, Length=293, Percent_Identity=25.2559726962457, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320242, Length=180, Percent_Identity=28.3333333333333, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24584738, Length=604, Percent_Identity=36.7549668874172, Blast_Score=355, Evalue=7e-98,
Organism=Drosophila melanogaster, GI24640849, Length=256, Percent_Identity=28.515625, Blast_Score=86, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24640851, Length=256, Percent_Identity=28.515625, Blast_Score=86, Evalue=9e-17,
Organism=Drosophila melanogaster, GI17136276, Length=262, Percent_Identity=26.3358778625954, Blast_Score=84, Evalue=4e-16,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYD_BACV8 (A6L585)

Other databases:

- EMBL:   CP000139
- RefSeq:   YP_001300471.1
- ProteinModelPortal:   A6L585
- SMR:   A6L585
- STRING:   A6L585
- GeneID:   5304181
- GenomeReviews:   CP000139_GR
- KEGG:   bvu:BVU_3220
- NMPDR:   fig|435590.6.peg.3002
- eggNOG:   COG0173
- HOGENOM:   HBG396032
- OMA:   AFPKTQQ
- ProtClustDB:   PRK00476
- GO:   GO:0005737
- HAMAP:   MF_00044_B
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:3.30.1360.30
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PANTHER:   PTHR22594:SF5
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00459

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.12

Molecular weight: Translated: 66452; Mature: 66452

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFRSHTCGELRLSDAGKSVTLAGWVQRARKMGGMTFVDLRDRYGITQLVFNEAVNAELCE
CCCCCCCCCEEECCCCCEEEHHHHHHHHHHHCCCEEEEEHHHCCHHHHHHHHCCCHHHHH
RANHLGREFVIQITGEVNERSNKNMNIPTGEIEIIVSVLNVLNSAVTPPFTIEDNSDGGD
HHHHCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCC
DIRMKFRYLDLRRNCVRKNLELRHKMTMEVRRYLDSKGFLEVETPMLVGSTPEGARDFVV
CEEEEEHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEEECCEEECCCCCCCCCCCC
PSRMNPGQFYALPQSPQTLKQLLMVSGFDRYFQIVKCFRDEDLRADRQPEFTQIDCEMSF
CCCCCCCCEEECCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCEEEECHHHH
VEQEDIISTFEGMAKHLFKELRGVELSEPFLRMTWADAIKYYGSDKPDLRFGMKFVELMD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
IMKGHGFSVFDDAAYIGGICAEGAASYTRKQLDQLTEFVKKPQIGAKGMVYARVEADGNV
HHHCCCCCEECCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCH
KSSVDKFYSQEVLQQMKEAFSAKPGDLILILSGPDAMKTRKQLCELRLEVGRQLGLRDKN
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCC
KFACLWVVDFPMFEWSEEEGRLMAMHHPFTHPKEEDIPLLDTDPAAVRADAYDMVVNGVE
CEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCEECCCCCHHCCCHHHHHEECEE
VGGGSIRIHDSQLQAKMFEILGFTPERAQEQFGFLMNAFKYGAPPHGGLAYGLDRWVSLF
ECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
AGLDSIRDCIAFPKNNSGRDVMLDAPAALDQSQLDELNLVVDIKEDK
HHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHCCEEEEEEEECCC
>Mature Secondary Structure
MFRSHTCGELRLSDAGKSVTLAGWVQRARKMGGMTFVDLRDRYGITQLVFNEAVNAELCE
CCCCCCCCCEEECCCCCEEEHHHHHHHHHHHCCCEEEEEHHHCCHHHHHHHHCCCHHHHH
RANHLGREFVIQITGEVNERSNKNMNIPTGEIEIIVSVLNVLNSAVTPPFTIEDNSDGGD
HHHHCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCC
DIRMKFRYLDLRRNCVRKNLELRHKMTMEVRRYLDSKGFLEVETPMLVGSTPEGARDFVV
CEEEEEHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEEECCEEECCCCCCCCCCCC
PSRMNPGQFYALPQSPQTLKQLLMVSGFDRYFQIVKCFRDEDLRADRQPEFTQIDCEMSF
CCCCCCCCEEECCCCHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCEEEECHHHH
VEQEDIISTFEGMAKHLFKELRGVELSEPFLRMTWADAIKYYGSDKPDLRFGMKFVELMD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
IMKGHGFSVFDDAAYIGGICAEGAASYTRKQLDQLTEFVKKPQIGAKGMVYARVEADGNV
HHHCCCCCEECCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCH
KSSVDKFYSQEVLQQMKEAFSAKPGDLILILSGPDAMKTRKQLCELRLEVGRQLGLRDKN
HHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCC
KFACLWVVDFPMFEWSEEEGRLMAMHHPFTHPKEEDIPLLDTDPAAVRADAYDMVVNGVE
CEEEEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCCCEECCCCCHHCCCHHHHHEECEE
VGGGSIRIHDSQLQAKMFEILGFTPERAQEQFGFLMNAFKYGAPPHGGLAYGLDRWVSLF
ECCCEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
AGLDSIRDCIAFPKNNSGRDVMLDAPAALDQSQLDELNLVVDIKEDK
HHHHHHHHHHCCCCCCCCCEEEEECCCCCCHHHHCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA