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The map label for this gene is folA

Identifier: 148828079

GI number: 148828079

Start: 1486709

End: 1487191

Strand: Reverse

Name: folA

Synonym: CGSHiGG_08055

Alternate gene names: 148828079

Gene position: 1487191-1486709 (Counterclockwise)

Preceding gene: 148828085

Following gene: 148828078

Centisome position: 78.8

GC content: 36.85

Gene sequence:

>483_bases
ATGACATTTAGTTTAATTGTGGCGACAACATTAAATAATGTGATTGGTAAAGATAACCAAATGCCTTGGCACTTGCCTGC
AGATTTAGCTTGGTTTCGTCAAAACACCACTGGTAAACCTGTCATTATGGGGCGTAAAACCTTTGAAAGTATTGGTCGTC
CACTACCTAAACGTACCAATATCGTACTTTCTCGCCAGCTTTTTGAACACGAAGGTGTGATATGGAAAGATAGCTTTGAA
AGTGCGGTCAATTTTGTCAGAGATTTTGATGAAATTATGTTGATTGGTGGGGGAGAGTTATTCAAACAATATTTACCCAA
AGCAGATAAGTTATACCTTACTCAAATTCAAACAGAACTAGATGGCGATACTTTTTTCCCTCAATTGAATTGGGAGGAGT
GGGAAATTGAATTTGATGAATATCGTAAGGCGGATGAACAAAATCGCTATGATTGTCGATTTTTGATTCTTACACGAAAA
TAA

Upstream 100 bases:

>100_bases
AGTCCTTTCCAAGTTCTTAAAATTGGCATTAACGTTATCACTAAATCAATGAAAAATCACTTAATATCAGGTATAGTAAC
GGCAAATTTTAGGGGGATTT

Downstream 100 bases:

>100_bases
ATCAAATTAATGAGACTATATCTTTAGAATATTCATTAATCTAGAAAATAAAGAGATGATGATTTCCCTTGAGTTCCCCT
TTTTAAAGTTTCATAATGAC

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MTFSLIVATTLNNVIGKDNQMPWHLPADLAWFRQNTTGKPVIMGRKTFESIGRPLPKRTNIVLSRQLFEHEGVIWKDSFE
SAVNFVRDFDEIMLIGGGELFKQYLPKADKLYLTQIQTELDGDTFFPQLNWEEWEIEFDEYRKADEQNRYDCRFLILTRK

Sequences:

>Translated_160_residues
MTFSLIVATTLNNVIGKDNQMPWHLPADLAWFRQNTTGKPVIMGRKTFESIGRPLPKRTNIVLSRQLFEHEGVIWKDSFE
SAVNFVRDFDEIMLIGGGELFKQYLPKADKLYLTQIQTELDGDTFFPQLNWEEWEIEFDEYRKADEQNRYDCRFLILTRK
>Mature_159_residues
TFSLIVATTLNNVIGKDNQMPWHLPADLAWFRQNTTGKPVIMGRKTFESIGRPLPKRTNIVLSRQLFEHEGVIWKDSFES
AVNFVRDFDEIMLIGGGELFKQYLPKADKLYLTQIQTELDGDTFFPQLNWEEWEIEFDEYRKADEQNRYDCRFLILTRK

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain

Homologues:

Organism=Homo sapiens, GI4503323, Length=156, Percent_Identity=32.6923076923077, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1786233, Length=158, Percent_Identity=53.1645569620253, Blast_Score=175, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI17505899, Length=153, Percent_Identity=33.3333333333333, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324810, Length=123, Percent_Identity=34.9593495934959, Blast_Score=69, Evalue=3e-13,
Organism=Drosophila melanogaster, GI116008026, Length=147, Percent_Identity=35.3741496598639, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24647458, Length=147, Percent_Identity=35.3741496598639, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): DYR_HAEIN (P43791)

Other databases:

- EMBL:   L42023
- EMBL:   X84207
- EMBL:   X84205
- EMBL:   X84206
- PIR:   C64101
- PIR:   S52336
- PIR:   S52337
- PIR:   S52338
- RefSeq:   NP_439060.1
- ProteinModelPortal:   P43791
- SMR:   P43791
- GeneID:   949905
- GenomeReviews:   L42023_GR
- KEGG:   hin:HI0899
- TIGR:   HI_0899
- HOGENOM:   HBG665708
- OMA:   APNTFGY
- ProtClustDB:   CLSK870655
- BioCyc:   HINF71421:HI_0899-MONOMER
- BRENDA:   1.5.1.3
- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796
- PANTHER:   PTHR11549:SF1
- PRINTS:   PR00070

Pfam domain/function: PF00186 DHFR_1

EC number: =1.5.1.3

Molecular weight: Translated: 18913; Mature: 18782

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00075 DHFR_1; PS51330 DHFR_2

Important sites: BINDING 6-6 BINDING 8-8 BINDING 28-28 BINDING 53-53 BINDING 58-58 BINDING 114-114

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFSLIVATTLNNVIGKDNQMPWHLPADLAWFRQNTTGKPVIMGRKTFESIGRPLPKRTN
CCEEEEHHHHHHHHHCCCCCCCEECCCHHHHHHCCCCCCEEEECHHHHHHHCCCCCCCCC
IVLSRQLFEHEGVIWKDSFESAVNFVRDFDEIMLIGGGELFKQYLPKADKLYLTQIQTEL
HHHHHHHHHHCCCEEECCHHHHHHHHHHHHHEEEECCHHHHHHHCCCCCCEEEEHHHHHC
DGDTFFPQLNWEEWEIEFDEYRKADEQNRYDCRFLILTRK
CCCCCCCCCCCCEEEEEHHHHHCCCCCCCCCEEEEEEECC
>Mature Secondary Structure 
TFSLIVATTLNNVIGKDNQMPWHLPADLAWFRQNTTGKPVIMGRKTFESIGRPLPKRTN
CEEEEHHHHHHHHHCCCCCCCEECCCHHHHHHCCCCCCEEEECHHHHHHHCCCCCCCCC
IVLSRQLFEHEGVIWKDSFESAVNFVRDFDEIMLIGGGELFKQYLPKADKLYLTQIQTEL
HHHHHHHHHHCCCEEECCHHHHHHHHHHHHHEEEECCHHHHHHHCCCCCCEEEEHHHHHC
DGDTFFPQLNWEEWEIEFDEYRKADEQNRYDCRFLILTRK
CCCCCCCCCCCCEEEEEHHHHHCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800