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The map label for this gene is rlmB [H]

Identifier: 148828043

GI number: 148828043

Start: 1439631

End: 1440371

Strand: Reverse

Name: rlmB [H]

Synonym: CGSHiGG_07820

Alternate gene names: 148828043

Gene position: 1440371-1439631 (Counterclockwise)

Preceding gene: 148828044

Following gene: 148828039

Centisome position: 76.32

GC content: 42.65

Gene sequence:

>741_bases
ATGTCAGAACAAATCTACGGCATTCATGCTGTTAATAGTATTTTAACCCATTCGCCAGAACGTTTAATTGAAGTCTTCGT
ACTTAAAGGACGTGAAGATAAACGCCTACAGCCACTCTTAAACGAACTGTATTCCCTCGGAATTGGTGTGCAATTCGTTA
ATCGACAAACACTTGATAAAAAAGCCGATGGTGAAGTTCATCAGGGGATTATTGCTCGAGTACAAGCGGCAAAAGAACTC
AATGAAAATGATCTTGATGAAATTCTTTCGAATAAACAAAATCCGCTTGTATTAGTTTTGGATGGCGTGACGGATCCACA
CAACTTGGGAGCATGTTTACGAACAGCTGATGCAGCGGGCGCAGAGGCAGTGATCGTACCGAAAGATAAATCCGCACAAT
TAACATCTATTGTGCGTAAAGTTGCTTGTGGCGCGGCAGAAACCGTGCCGTTAATTCGTGTAACCAATCTTTCTCGCACA
CTGCGCGATTTACAACAAAACCATAATATTTGGGTGGTTGGCACTGCTGGCGAAGCCACTGAAACCATTTATCAAAGCAA
ACTGACAGGGCCACTAGCACTTGTTATGGGAGCTGAAGGCGAAGGGATGCGTCGTTTAACACGTGAACATTGCGATCAAC
TGATTAGCATTCCAATGGCTGGTTCAGTTTCATCATTAAATGTATCTGTCGCAACAGGGGTATGTTTATTTGAAATTGTA
CGTCAGCGTTTAGGTTCATAA

Upstream 100 bases:

>100_bases
AAAAAGAAAGAAGTTTCGAAGAAAACATCTAGAAAACAAAAATAATTCACTTCCTATTGTAAGAATGAATTAAAAATTCA
AATAAGAAAAAGAGAAATTT

Downstream 100 bases:

>100_bases
GAAAAGTGCGGTAAATTTACCGCACTTTTTTAATACCTATTGTCTCGCTTGAACTTCTGCGTTGGCATAATCAATGGTGA
CAATCTTACCAGGTAATAAC

Product: 23S rRNA (guanosine-2'-O-)-methyltransferase

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MSEQIYGIHAVNSILTHSPERLIEVFVLKGREDKRLQPLLNELYSLGIGVQFVNRQTLDKKADGEVHQGIIARVQAAKEL
NENDLDEILSNKQNPLVLVLDGVTDPHNLGACLRTADAAGAEAVIVPKDKSAQLTSIVRKVACGAAETVPLIRVTNLSRT
LRDLQQNHNIWVVGTAGEATETIYQSKLTGPLALVMGAEGEGMRRLTREHCDQLISIPMAGSVSSLNVSVATGVCLFEIV
RQRLGS

Sequences:

>Translated_246_residues
MSEQIYGIHAVNSILTHSPERLIEVFVLKGREDKRLQPLLNELYSLGIGVQFVNRQTLDKKADGEVHQGIIARVQAAKEL
NENDLDEILSNKQNPLVLVLDGVTDPHNLGACLRTADAAGAEAVIVPKDKSAQLTSIVRKVACGAAETVPLIRVTNLSRT
LRDLQQNHNIWVVGTAGEATETIYQSKLTGPLALVMGAEGEGMRRLTREHCDQLISIPMAGSVSSLNVSVATGVCLFEIV
RQRLGS
>Mature_245_residues
SEQIYGIHAVNSILTHSPERLIEVFVLKGREDKRLQPLLNELYSLGIGVQFVNRQTLDKKADGEVHQGIIARVQAAKELN
ENDLDEILSNKQNPLVLVLDGVTDPHNLGACLRTADAAGAEAVIVPKDKSAQLTSIVRKVACGAAETVPLIRVTNLSRTL
RDLQQNHNIWVVGTAGEATETIYQSKLTGPLALVMGAEGEGMRRLTREHCDQLISIPMAGSVSSLNVSVATGVCLFEIVR
QRLGS

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=233, Percent_Identity=33.0472103004292, Blast_Score=88, Evalue=7e-18,
Organism=Escherichia coli, GI1790623, Length=243, Percent_Identity=71.6049382716049, Blast_Score=367, Evalue=1e-103,
Organism=Escherichia coli, GI1788935, Length=240, Percent_Identity=27.9166666666667, Blast_Score=84, Evalue=6e-18,
Organism=Escherichia coli, GI1790083, Length=162, Percent_Identity=33.3333333333333, Blast_Score=68, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17555908, Length=259, Percent_Identity=28.957528957529, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6324775, Length=175, Percent_Identity=30.8571428571429, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26676; Mature: 26545

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQIYGIHAVNSILTHSPERLIEVFVLKGREDKRLQPLLNELYSLGIGVQFVNRQTLDK
CCCCEEHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEECCHHHCCC
KADGEVHQGIIARVQAAKELNENDLDEILSNKQNPLVLVLDGVTDPHNLGACLRTADAAG
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCCC
AEAVIVPKDKSAQLTSIVRKVACGAAETVPLIRVTNLSRTLRDLQQNHNIWVVGTAGEAT
CCEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHCCCEEEEECCCHHH
ETIYQSKLTGPLALVMGAEGEGMRRLTREHCDQLISIPMAGSVSSLNVSVATGVCLFEIV
HHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHH
RQRLGS
HHHHCC
>Mature Secondary Structure 
SEQIYGIHAVNSILTHSPERLIEVFVLKGREDKRLQPLLNELYSLGIGVQFVNRQTLDK
CCCEEHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEECCHHHCCC
KADGEVHQGIIARVQAAKELNENDLDEILSNKQNPLVLVLDGVTDPHNLGACLRTADAAG
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHCCCC
AEAVIVPKDKSAQLTSIVRKVACGAAETVPLIRVTNLSRTLRDLQQNHNIWVVGTAGEAT
CCEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHCCCEEEEECCCHHH
ETIYQSKLTGPLALVMGAEGEGMRRLTREHCDQLISIPMAGSVSSLNVSVATGVCLFEIV
HHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHH
RQRLGS
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]