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The map label for this gene is clpB [H]

Identifier: 148828042

GI number: 148828042

Start: 1437024

End: 1439594

Strand: Direct

Name: clpB [H]

Synonym: CGSHiGG_07815

Alternate gene names: 148828042

Gene position: 1437024-1439594 (Clockwise)

Preceding gene: 148828041

Following gene: 148828046

Centisome position: 76.15

GC content: 40.92

Gene sequence:

>2571_bases
ATGAACATTGAAAAATTTACGACAAAATTCCAAGAAGCGTTAAGCGAAGCACAATCACTTGCTATCGGAAAAGACAATCA
ATTTATTGAACCTGTGCATTTATTGACCGCACTTTTAAATCAACAGGGCGGCTCTATTGCGCCAATTTTGACAGCAAGTG
GCGTAAATGTGGCTTTATTGCGTAATGAATTAAAGACAGAACTAAATAAATTGCCGCAAGTGATTGGCAATGGTGGAGAT
GTACAACTTTCACGCCAGCTTATTAATCTACTTAATTTATGCGATAAATTCGCACAGCAAAACCAAGATAAATTTATTTC
GAGCGAATTGTTTTTGCTTGCAGCTTTAGAAGAACGAGGAACGATCAGCGATATTTTGAAAAAGTGCTGTGCGAAAAAAG
AACAAATTTCGCAAGCTATTCAGCACATTAGAGGGGGACAAAACGTGAACGATCAAAATGCAGAAGAAAGCAGACAAGCT
CTTGAAAAATATACGATTGATTTAACCGCTCGTGCAGAAAGTGGCAAACTTGATCCTGTAATTGGGCGTGATGAAGAAAT
TCGTCGAGCTATTCAAGTATTACAACGTCGTACCAAAAATAACCCAGTGTTAATTGGTGAACCAGGTGTAGGGAAAACGG
CGATTGTCGAAGGCTTGGCACAACGCATTGTAAATGGCGAAGTGCCAGAAGGTTTGAAAAATAAACGTGTGCTTTCATTA
GATATGGGCGCATTGATTGCTGGTGCAAAATATCGCGGAGAATTTGAAGAACGCTTAAAAGCTGTTTTAAATGAGTTATC
AAAAGAAGAAGGTCGCGTGATCCTCTTTATTGACGAAATTCATACTATGGTTGGCGCGGGTAAAACAGATGGCGCAATGG
ACGCAGGTAACTTGTTAAAACCAAGTTTGGCACGAGGCGAATTACATTGCGTGGGTGCAACTACTTTAGATGAATATCGT
CAATATATCGAAAAAGATGCCGCACTTGAGCGTCGTTTCCAAAAAGTCTTTGTGGACGAGCCTAGTGTAGAAGATACCAT
TGCGATCTTACGTGGTTTGAAAGAACGCTATGAAATTCATCATCACGTAGATATTACTGACCCAGCAATCGTAGCAGCGG
CAACGCTTTCACACCGTTATATTTCCGATCGTCAGTTACCAGATAAAGCGATTGATTTGATCGATGAAGCAGCGTCTAGC
ATTCGTATGGAAATAGATTCTAAACCTGAACCGCTTGATCGTCTTGAACGTCGTATTATCCAATTAAAATTGGAACAACA
AGCGTTACAAAAAGAAGAAGACGAAGCAAGTCGCAAACGTTTAGAAATGTTAGAGAAAGAATTGGCTGAAAAAGAACGTG
AATACGCCGAACTTGAAGAAGTGTGGAAATCTGAAAAAGCAACGCTTTCTGGCTCTCAACATATTAAACAAGAGTTAGAT
ACTGCAAAAACCGAACTAGAACAAGCTCGTCGCGCGGGTGATTTAGCGAAAATGTCAGAGTTGCAATATGGCCGCATCCC
TGCTCTTGAAAAGCAACTTGAGCAAGCTGAAACCAGCGAAGGAAAAGAAATGACGCTTTTACGCTATCGCGTCACAGATG
AAGAAATCGCAGAAGTGCTTTCTAAAGCCACTGGTATTCCTGTATCCAAAATGATGGAAGGCGAGAAAGAAAAACTCTTA
CGTATGGAGGATGAACTACATAAACGAGTGATTGGTCAAGAAGAAGCAGTTGATGCGGTAGCAAACGCGATTCGTCGTCG
TCGTGCTGGTCTTTCCGATCCTAATCGCCCAATTGGTTCTTTCTTGTTCCTAGGGCCAACAGGAGTGGGGAAAACAGAGC
TTTGCAAAACTTTGGCTAAATTCTTGTTTGATAGTGAAGATGCGATGGTGCGCATTGATATGTCAGAGTTTATGGAAAAA
CACAGTGTTTCTCGTTTAGTTGGCGCGCCTCCAGGCTATGTCGGCTATGAAGAAGGCGGTTATTTAACTGAAGCTGTTCG
TCGTCGTCCATATTCAGTGATCTTATTAGATGAAGTTGAAAAAGCCCATGCAGATGTATTCAATATTTTATTACAAGTGT
TGGATGATGGTCGTTTAACTGATGGTCAAGGTCGTACTGTGGACTTCCGTAACACTGTGGTTATTATGACCTCTAACTTG
GGTTCTGATTTAATCCAAGGTAATAAAGACGAAAGCTATAGCGAAATGAAAGCCTTAGTGATGTCAGTGGTAAGCCAACA
TTTCCGCCCAGAATTTATCAATCGTATTGACGAAACCGTGGTATTCCATCCACTTGGTAAAGAAAATATCCGTGCGATTG
CAAGTATCCAATTAGAACGCTTAGCAAAACGTATGGAAACTCGTGGCTATGAATTAGTGTTTACCGATGCTTTATTAGAT
TTCATTGGTGAAGTGGGGTATGACCCAATTTATGGTGCACGTCCATTGAAACGTGCTATTCAACAAGAAATCGAAAACAG
CTTGGCACAACAAATTCTATCTGGTGCGTTATTACCTGGTAAGATTGTCACCATTGATTATGCCAACGCAGAAGTTCAAG
CGAGACAATAG

Upstream 100 bases:

>100_bases
ATAAAATCAAAATATTTCATTTTTTCTCTTGAAATTTATTCAACCGAGCTTACTTAGTTTATGCAAATGGCGAAAGCCGA
AATCAAATTGAGGAAAGTGT

Downstream 100 bases:

>100_bases
GTATTAAAAAAGTGCGGTAAATTTACCGCACTTTTCTTATGAACCTAAACGCTGACGTACAATTTCAAATAAACATACCC
CTGTTGCGACAGATACATTT

Product: DNA polymerase I

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 856; Mature: 856

Protein sequence:

>856_residues
MNIEKFTTKFQEALSEAQSLAIGKDNQFIEPVHLLTALLNQQGGSIAPILTASGVNVALLRNELKTELNKLPQVIGNGGD
VQLSRQLINLLNLCDKFAQQNQDKFISSELFLLAALEERGTISDILKKCCAKKEQISQAIQHIRGGQNVNDQNAEESRQA
LEKYTIDLTARAESGKLDPVIGRDEEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPEGLKNKRVLSL
DMGALIAGAKYRGEFEERLKAVLNELSKEEGRVILFIDEIHTMVGAGKTDGAMDAGNLLKPSLARGELHCVGATTLDEYR
QYIEKDAALERRFQKVFVDEPSVEDTIAILRGLKERYEIHHHVDITDPAIVAAATLSHRYISDRQLPDKAIDLIDEAASS
IRMEIDSKPEPLDRLERRIIQLKLEQQALQKEEDEASRKRLEMLEKELAEKEREYAELEEVWKSEKATLSGSQHIKQELD
TAKTELEQARRAGDLAKMSELQYGRIPALEKQLEQAETSEGKEMTLLRYRVTDEEIAEVLSKATGIPVSKMMEGEKEKLL
RMEDELHKRVIGQEEAVDAVANAIRRRRAGLSDPNRPIGSFLFLGPTGVGKTELCKTLAKFLFDSEDAMVRIDMSEFMEK
HSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKAHADVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
GSDLIQGNKDESYSEMKALVMSVVSQHFRPEFINRIDETVVFHPLGKENIRAIASIQLERLAKRMETRGYELVFTDALLD
FIGEVGYDPIYGARPLKRAIQQEIENSLAQQILSGALLPGKIVTIDYANAEVQARQ

Sequences:

>Translated_856_residues
MNIEKFTTKFQEALSEAQSLAIGKDNQFIEPVHLLTALLNQQGGSIAPILTASGVNVALLRNELKTELNKLPQVIGNGGD
VQLSRQLINLLNLCDKFAQQNQDKFISSELFLLAALEERGTISDILKKCCAKKEQISQAIQHIRGGQNVNDQNAEESRQA
LEKYTIDLTARAESGKLDPVIGRDEEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPEGLKNKRVLSL
DMGALIAGAKYRGEFEERLKAVLNELSKEEGRVILFIDEIHTMVGAGKTDGAMDAGNLLKPSLARGELHCVGATTLDEYR
QYIEKDAALERRFQKVFVDEPSVEDTIAILRGLKERYEIHHHVDITDPAIVAAATLSHRYISDRQLPDKAIDLIDEAASS
IRMEIDSKPEPLDRLERRIIQLKLEQQALQKEEDEASRKRLEMLEKELAEKEREYAELEEVWKSEKATLSGSQHIKQELD
TAKTELEQARRAGDLAKMSELQYGRIPALEKQLEQAETSEGKEMTLLRYRVTDEEIAEVLSKATGIPVSKMMEGEKEKLL
RMEDELHKRVIGQEEAVDAVANAIRRRRAGLSDPNRPIGSFLFLGPTGVGKTELCKTLAKFLFDSEDAMVRIDMSEFMEK
HSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKAHADVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
GSDLIQGNKDESYSEMKALVMSVVSQHFRPEFINRIDETVVFHPLGKENIRAIASIQLERLAKRMETRGYELVFTDALLD
FIGEVGYDPIYGARPLKRAIQQEIENSLAQQILSGALLPGKIVTIDYANAEVQARQ
>Mature_856_residues
MNIEKFTTKFQEALSEAQSLAIGKDNQFIEPVHLLTALLNQQGGSIAPILTASGVNVALLRNELKTELNKLPQVIGNGGD
VQLSRQLINLLNLCDKFAQQNQDKFISSELFLLAALEERGTISDILKKCCAKKEQISQAIQHIRGGQNVNDQNAEESRQA
LEKYTIDLTARAESGKLDPVIGRDEEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPEGLKNKRVLSL
DMGALIAGAKYRGEFEERLKAVLNELSKEEGRVILFIDEIHTMVGAGKTDGAMDAGNLLKPSLARGELHCVGATTLDEYR
QYIEKDAALERRFQKVFVDEPSVEDTIAILRGLKERYEIHHHVDITDPAIVAAATLSHRYISDRQLPDKAIDLIDEAASS
IRMEIDSKPEPLDRLERRIIQLKLEQQALQKEEDEASRKRLEMLEKELAEKEREYAELEEVWKSEKATLSGSQHIKQELD
TAKTELEQARRAGDLAKMSELQYGRIPALEKQLEQAETSEGKEMTLLRYRVTDEEIAEVLSKATGIPVSKMMEGEKEKLL
RMEDELHKRVIGQEEAVDAVANAIRRRRAGLSDPNRPIGSFLFLGPTGVGKTELCKTLAKFLFDSEDAMVRIDMSEFMEK
HSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKAHADVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
GSDLIQGNKDESYSEMKALVMSVVSQHFRPEFINRIDETVVFHPLGKENIRAIASIQLERLAKRMETRGYELVFTDALLD
FIGEVGYDPIYGARPLKRAIQQEIENSLAQQILSGALLPGKIVTIDYANAEVQARQ

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=353, Percent_Identity=36.8271954674221, Blast_Score=209, Evalue=1e-53,
Organism=Escherichia coli, GI1788943, Length=857, Percent_Identity=77.3628938156359, Blast_Score=1365, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=398, Percent_Identity=40.9547738693467, Blast_Score=275, Evalue=8e-75,
Organism=Saccharomyces cerevisiae, GI6320464, Length=701, Percent_Identity=53.7803138373752, Blast_Score=768, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=858, Percent_Identity=42.8904428904429, Blast_Score=674, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 95805; Mature: 95805

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIEKFTTKFQEALSEAQSLAIGKDNQFIEPVHLLTALLNQQGGSIAPILTASGVNVALL
CCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHH
RNELKTELNKLPQVIGNGGDVQLSRQLINLLNLCDKFAQQNQDKFISSELFLLAALEERG
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
TISDILKKCCAKKEQISQAIQHIRGGQNVNDQNAEESRQALEKYTIDLTARAESGKLDPV
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHEEEEECCCCCCCCC
IGRDEEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPEGLKNKRVLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
DMGALIAGAKYRGEFEERLKAVLNELSKEEGRVILFIDEIHTMVGAGKTDGAMDAGNLLK
CHHHHHHCCHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCCCCC
PSLARGELHCVGATTLDEYRQYIEKDAALERRFQKVFVDEPSVEDTIAILRGLKERYEIH
CCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HHVDITDPAIVAAATLSHRYISDRQLPDKAIDLIDEAASSIRMEIDSKPEPLDRLERRII
HCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHH
QLKLEQQALQKEEDEASRKRLEMLEKELAEKEREYAELEEVWKSEKATLSGSQHIKQELD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TAKTELEQARRAGDLAKMSELQYGRIPALEKQLEQAETSEGKEMTLLRYRVTDEEIAEVL
HHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHH
SKATGIPVSKMMEGEKEKLLRMEDELHKRVIGQEEAVDAVANAIRRRRAGLSDPNRPIGS
HHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
FLFLGPTGVGKTELCKTLAKFLFDSEDAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLTEAVRRRPYSVILLDEVEKAHADVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
HHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCC
GSDLIQGNKDESYSEMKALVMSVVSQHFRPEFINRIDETVVFHPLGKENIRAIASIQLER
CHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCCHHHHHHHHHHHHHH
LAKRMETRGYELVFTDALLDFIGEVGYDPIYGARPLKRAIQQEIENSLAQQILSGALLPG
HHHHHHHCCCEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
KIVTIDYANAEVQARQ
EEEEEEECCCCCCCCC
>Mature Secondary Structure
MNIEKFTTKFQEALSEAQSLAIGKDNQFIEPVHLLTALLNQQGGSIAPILTASGVNVALL
CCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHH
RNELKTELNKLPQVIGNGGDVQLSRQLINLLNLCDKFAQQNQDKFISSELFLLAALEERG
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
TISDILKKCCAKKEQISQAIQHIRGGQNVNDQNAEESRQALEKYTIDLTARAESGKLDPV
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHEEEEECCCCCCCCC
IGRDEEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEGLAQRIVNGEVPEGLKNKRVLSL
CCCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCEEEEE
DMGALIAGAKYRGEFEERLKAVLNELSKEEGRVILFIDEIHTMVGAGKTDGAMDAGNLLK
CHHHHHHCCHHCCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCCCCCCCCCCCCC
PSLARGELHCVGATTLDEYRQYIEKDAALERRFQKVFVDEPSVEDTIAILRGLKERYEIH
CCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
HHVDITDPAIVAAATLSHRYISDRQLPDKAIDLIDEAASSIRMEIDSKPEPLDRLERRII
HCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHH
QLKLEQQALQKEEDEASRKRLEMLEKELAEKEREYAELEEVWKSEKATLSGSQHIKQELD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TAKTELEQARRAGDLAKMSELQYGRIPALEKQLEQAETSEGKEMTLLRYRVTDEEIAEVL
HHHHHHHHHHHHCCHHHHHHCCCCCCHHHHHHHHHHHCCCCCCEEEEEEECCHHHHHHHH
SKATGIPVSKMMEGEKEKLLRMEDELHKRVIGQEEAVDAVANAIRRRRAGLSDPNRPIGS
HHHCCCCHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
FLFLGPTGVGKTELCKTLAKFLFDSEDAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGG
EEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCC
YLTEAVRRRPYSVILLDEVEKAHADVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNL
HHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEEECCC
GSDLIQGNKDESYSEMKALVMSVVSQHFRPEFINRIDETVVFHPLGKENIRAIASIQLER
CHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCCHHHHHHHHHHHHHH
LAKRMETRGYELVFTDALLDFIGEVGYDPIYGARPLKRAIQQEIENSLAQQILSGALLPG
HHHHHHHCCCEEEEHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
KIVTIDYANAEVQARQ
EEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]