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The map label for this gene is slt [H]

Identifier: 148828015

GI number: 148828015

Start: 1409464

End: 1411248

Strand: Direct

Name: slt [H]

Synonym: CGSHiGG_07650

Alternate gene names: 148828015

Gene position: 1409464-1411248 (Clockwise)

Preceding gene: 148828014

Following gene: 148828016

Centisome position: 74.69

GC content: 35.29

Gene sequence:

>1785_bases
ATGAAAAAAGTCGCCTTAATTTCTCTTTGTATTTTTACCGCACTTTCTGCTTTTGCCGATTTGCCTAATACGGCAACAGC
ATCATCAATCAATCTTGAGCAAGAGAAACAAAACTGGGCGTCAATACAGCATCAAGATTATTTAAAACGTCTAAAACAAC
GTGAAGTCTTTTTGCAAGTTGAAGGTTTGCTAAAAAGTGCGGTAAAAAAACAGCAATTTTCTGAAGCGACTCAAAATATC
ACAAAAACCTTAATAGACTCATTGCAAGGCTACCCTTTGCAATATGATCTGCTTGCTCGATTTTGGGAGACAAAAATCGC
CTTTTTGCAGAATGATGATATTCAAGGAAAACAACAAGCAATAAATGAACTCAATGCGTTAGTGCAACAAAATTACCCTT
TTGTGACACCAGCATTTCAAGCCTTATTACAAAAACTATCCACGTTAAACGAACAACAAACATCAGCAACTTCAGATAAT
GCTAAAGAAAATAACAGGGTTCAAAAAGAACAAAATCAAGTAGAAAATCCTAAACAACTTGCTGAAATTGTAAGAAAAAG
TGATCCTAACACTTTAGATAAAACCGTATTAATTGATGCTTTCCCACGTTATCTTAAAACGCTACCTGAACAAATGAATA
ACCTAAGTTTTGAATCCTATCAAAAATGGGCAAATACTTGGCTTCTCTCAGAAGATGAAATCAAACAGTGGAAAATAGCC
TTTTTAAATCGCTTTTTTGATAATGAAAACGCTGATTTTCAAAAATGGCGTGATGAACAAATCCGACAATTACAGACTGA
TAATTTAACCGAACGCCGTTTACGAATGGCTATTTGGCAAAAAACTGAATTAACCTCTTGGCTAAATTTGCTTTCTGCCG
AATCAAAAAGTAAGCAAGAATGGCGTTATTGGGAAGCTAAACAAGATATATTAAAAAATACCAAAAAACTGACCGCACTT
TCAAAAGAGCGTGGCTTTTATCCAATGTTAGCGGCTACACAATTAAAACAAGCATATCAACTGAATGTCCCTATTGCGTC
AAGTTTTACTCAAGCTGAACAACTTCCTTTTAAGCAAGTTTTTGCAATGATTACAGAATTAAGAGAACTTGGACGAAATG
GTTTAGCAAAACAACGCTGGCGAATTTTACTTGATAATGTTGATTTTACAACTCAGCTAAAACTTTCGGAATATGCCAAA
AATCAGCAATGGTTTGAATTAGCTGTTGATGCTTCTATTGTGGCAAAAGCGTGGGATTACCTGTCTCTTCGTCTGCCAAA
TGCTTATTCTGAATACTTCAATGCTGCATTACAGAATTTGAATATCAGCAAAACTTTTGCTATGGCAATCGCTCGTCAAG
AAAGTGCTTGGAATCCAATGGCACAATCTTCAGCAAATGCTCGAGGCTTAATGCAGCTTTTGCCAAGTACGGCAAAATTG
ACTGCAGAGAATAATCAACTACCTTATCAAGGCGAACAAGATTTATTCAAACCATTGAATAATATTTTGCTAGGTACCGC
ACATCTTAATGAACTCAATGGAAAATATCCTAATAATCGAATATTGATTGCAGCCGCTTACAATGCAGGGGCAAATCGAG
TAGAAAAATGGTTAAGTCGTGCGAGTGGAAAATTAGCATTAGATGAATTTGTTGCATCTATTCCTTTTTATGAAACTCGT
GGTTATGTGCAAAATGTAGTCGCTTATGATTTTTACTATCAAATTTTGCAAAATAAAGAAAACCCACAAATCTTTAGCCA
AGAAGAATTGAATCGGCTATACTAA

Upstream 100 bases:

>100_bases
GAAAGACTGGGCAGCACAAGATCCTTATGTAGAAGCTGGCGTATATGCGGATGTTATTGTAAAGCCGTTTAAAAAAGTCT
TTTAATTTAAATAACGTAAT

Downstream 100 bases:

>100_bases
ATGCACTAGTTTAATAGTGTAAAATGGGAGCATAATTATGTATATCAGCCGTAATTTAGAGCAATGGAATGCATTTCTTC
AAATGCTAAAAATCGCCTTT

Product: intracellular septation protein A

Products: 1,6-Anhydrobond [C]

Alternate protein names: Peptidoglycan lytic exotransglycosylase [H]

Number of amino acids: Translated: 594; Mature: 594

Protein sequence:

>594_residues
MKKVALISLCIFTALSAFADLPNTATASSINLEQEKQNWASIQHQDYLKRLKQREVFLQVEGLLKSAVKKQQFSEATQNI
TKTLIDSLQGYPLQYDLLARFWETKIAFLQNDDIQGKQQAINELNALVQQNYPFVTPAFQALLQKLSTLNEQQTSATSDN
AKENNRVQKEQNQVENPKQLAEIVRKSDPNTLDKTVLIDAFPRYLKTLPEQMNNLSFESYQKWANTWLLSEDEIKQWKIA
FLNRFFDNENADFQKWRDEQIRQLQTDNLTERRLRMAIWQKTELTSWLNLLSAESKSKQEWRYWEAKQDILKNTKKLTAL
SKERGFYPMLAATQLKQAYQLNVPIASSFTQAEQLPFKQVFAMITELRELGRNGLAKQRWRILLDNVDFTTQLKLSEYAK
NQQWFELAVDASIVAKAWDYLSLRLPNAYSEYFNAALQNLNISKTFAMAIARQESAWNPMAQSSANARGLMQLLPSTAKL
TAENNQLPYQGEQDLFKPLNNILLGTAHLNELNGKYPNNRILIAAAYNAGANRVEKWLSRASGKLALDEFVASIPFYETR
GYVQNVVAYDFYYQILQNKENPQIFSQEELNRLY

Sequences:

>Translated_594_residues
MKKVALISLCIFTALSAFADLPNTATASSINLEQEKQNWASIQHQDYLKRLKQREVFLQVEGLLKSAVKKQQFSEATQNI
TKTLIDSLQGYPLQYDLLARFWETKIAFLQNDDIQGKQQAINELNALVQQNYPFVTPAFQALLQKLSTLNEQQTSATSDN
AKENNRVQKEQNQVENPKQLAEIVRKSDPNTLDKTVLIDAFPRYLKTLPEQMNNLSFESYQKWANTWLLSEDEIKQWKIA
FLNRFFDNENADFQKWRDEQIRQLQTDNLTERRLRMAIWQKTELTSWLNLLSAESKSKQEWRYWEAKQDILKNTKKLTAL
SKERGFYPMLAATQLKQAYQLNVPIASSFTQAEQLPFKQVFAMITELRELGRNGLAKQRWRILLDNVDFTTQLKLSEYAK
NQQWFELAVDASIVAKAWDYLSLRLPNAYSEYFNAALQNLNISKTFAMAIARQESAWNPMAQSSANARGLMQLLPSTAKL
TAENNQLPYQGEQDLFKPLNNILLGTAHLNELNGKYPNNRILIAAAYNAGANRVEKWLSRASGKLALDEFVASIPFYETR
GYVQNVVAYDFYYQILQNKENPQIFSQEELNRLY
>Mature_594_residues
MKKVALISLCIFTALSAFADLPNTATASSINLEQEKQNWASIQHQDYLKRLKQREVFLQVEGLLKSAVKKQQFSEATQNI
TKTLIDSLQGYPLQYDLLARFWETKIAFLQNDDIQGKQQAINELNALVQQNYPFVTPAFQALLQKLSTLNEQQTSATSDN
AKENNRVQKEQNQVENPKQLAEIVRKSDPNTLDKTVLIDAFPRYLKTLPEQMNNLSFESYQKWANTWLLSEDEIKQWKIA
FLNRFFDNENADFQKWRDEQIRQLQTDNLTERRLRMAIWQKTELTSWLNLLSAESKSKQEWRYWEAKQDILKNTKKLTAL
SKERGFYPMLAATQLKQAYQLNVPIASSFTQAEQLPFKQVFAMITELRELGRNGLAKQRWRILLDNVDFTTQLKLSEYAK
NQQWFELAVDASIVAKAWDYLSLRLPNAYSEYFNAALQNLNISKTFAMAIARQESAWNPMAQSSANARGLMQLLPSTAKL
TAENNQLPYQGEQDLFKPLNNILLGTAHLNELNGKYPNNRILIAAAYNAGANRVEKWLSRASGKLALDEFVASIPFYETR
GYVQNVVAYDFYYQILQNKENPQIFSQEELNRLY

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=382, Percent_Identity=39.2670157068063, Blast_Score=258, Evalue=8e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011989
- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 68752; Mature: 68752

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVALISLCIFTALSAFADLPNTATASSINLEQEKQNWASIQHQDYLKRLKQREVFLQV
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGLLKSAVKKQQFSEATQNITKTLIDSLQGYPLQYDLLARFWETKIAFLQNDDIQGKQQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHH
INELNALVQQNYPFVTPAFQALLQKLSTLNEQQTSATSDNAKENNRVQKEQNQVENPKQL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHH
AEIVRKSDPNTLDKTVLIDAFPRYLKTLPEQMNNLSFESYQKWANTWLLSEDEIKQWKIA
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHH
FLNRFFDNENADFQKWRDEQIRQLQTDNLTERRLRMAIWQKTELTSWLNLLSAESKSKQE
HHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
WRYWEAKQDILKNTKKLTALSKERGFYPMLAATQLKQAYQLNVPIASSFTQAEQLPFKQV
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHH
FAMITELRELGRNGLAKQRWRILLDNVDFTTQLKLSEYAKNQQWFELAVDASIVAKAWDY
HHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEHHHHHHCCCHHHHHHHHHHHHHHHHHH
LSLRLPNAYSEYFNAALQNLNISKTFAMAIARQESAWNPMAQSSANARGLMQLLPSTAKL
HEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHCCCHHEE
TAENNQLPYQGEQDLFKPLNNILLGTAHLNELNGKYPNNRILIAAAYNAGANRVEKWLSR
EECCCCCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHH
ASGKLALDEFVASIPFYETRGYVQNVVAYDFYYQILQNKENPQIFSQEELNRLY
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCC
>Mature Secondary Structure
MKKVALISLCIFTALSAFADLPNTATASSINLEQEKQNWASIQHQDYLKRLKQREVFLQV
CCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EGLLKSAVKKQQFSEATQNITKTLIDSLQGYPLQYDLLARFWETKIAFLQNDDIQGKQQA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHH
INELNALVQQNYPFVTPAFQALLQKLSTLNEQQTSATSDNAKENNRVQKEQNQVENPKQL
HHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHH
AEIVRKSDPNTLDKTVLIDAFPRYLKTLPEQMNNLSFESYQKWANTWLLSEDEIKQWKIA
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHH
FLNRFFDNENADFQKWRDEQIRQLQTDNLTERRLRMAIWQKTELTSWLNLLSAESKSKQE
HHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
WRYWEAKQDILKNTKKLTALSKERGFYPMLAATQLKQAYQLNVPIASSFTQAEQLPFKQV
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHH
FAMITELRELGRNGLAKQRWRILLDNVDFTTQLKLSEYAKNQQWFELAVDASIVAKAWDY
HHHHHHHHHHCCCCCHHHHHHHHHCCCCCCEEEHHHHHHCCCHHHHHHHHHHHHHHHHHH
LSLRLPNAYSEYFNAALQNLNISKTFAMAIARQESAWNPMAQSSANARGLMQLLPSTAKL
HEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHCCCHHEE
TAENNQLPYQGEQDLFKPLNNILLGTAHLNELNGKYPNNRILIAAAYNAGANRVEKWLSR
EECCCCCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHHHHH
ASGKLALDEFVASIPFYETRGYVQNVVAYDFYYQILQNKENPQIFSQEELNRLY
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]