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The map label for this gene is folD [H]

Identifier: 148827900

GI number: 148827900

Start: 1285651

End: 1286499

Strand: Reverse

Name: folD [H]

Synonym: CGSHiGG_06985

Alternate gene names: 148827900

Gene position: 1286499-1285651 (Counterclockwise)

Preceding gene: 148827901

Following gene: 148827895

Centisome position: 68.17

GC content: 39.22

Gene sequence:

>849_bases
ATGGCCGCAAAAATCATCTCTGGTACTGAACTTTCAAAACAAATTAAAGCTAATTTAGCAGATAAAATAACCCATTATAT
TGAACAAGGAAAACGCGTTCCAGGACTTGCTGTAATCTTAGTTGGTGCTGATCCTGCTTCGCAAATTTATGTGGGCAATA
AACGTAAAAGTTGCGAGGAAGTCGGTATTCTCTCTAAATCTTATGACCTCCCTGAAACAACCACGCAAAATGAATTATTA
GCGATTATTGACCAACTTAACGCAGATAAAAATATTGACGGTATTTTGGTGCAACTCCCCTTGCCAAAACAAATTAATGC
GGAAGCTATTATTGAACATATCGATCCTAAAAAAGATGTAGATGGCTTTCATCCTTATAATGTTGGGCGGTTATGCCAAC
GTATTCCAACTTTACGTGCTTGTACACCTTATGGTGTGATGAAACTATTAGAAACAACGGGTATTGATTTACACGGCAAG
CACGCAGTAATTGTAGGCGCATCCAATATTGTCGGCCGCCCAATGTCTTTAGAATTATTATTAGCAGGGGCAACCGTCAC
AGTAACACATAGATTCACTAAAAATTTGGAAAATCACGTTCGCCAAGCAGATATACTTGTCGTTGCGGTAGGTAAACCAA
ATTTAATTTCTGGCGATTGGATCAAAGAAAGTGCGGTAGTAATTGATGTTGGAATTAACCGTGTAGATGGAAAATTAGTA
GGCGATATAGAGTTTGATAAAGCAGCCGAAAAAGCGGCTTACATTACACCAGTCCCCGGTGGCGTTGGGCCAATGACCGT
TGCAATGTTGATGAGCAATACGCTTTATGCTTATGAACACAATAAATAG

Upstream 100 bases:

>100_bases
TCCACTTTAAGAAATATCCCCCTTTTTCATTGTAAAAAATCATTGTTCGTTAAAATAGCAAACGCTTTCTTTTTCATCAC
ATAATATCAAGGATAGCTCA

Downstream 100 bases:

>100_bases
ATTTGTATAGAACGGTTATGAAAAATGGGTTAAAGCTTCATTTGCTTTAACCCATTTATTTAAATGGCTTTATTGTACTT
CTTACGTTTAAATTTATGAT

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MAAKIISGTELSKQIKANLADKITHYIEQGKRVPGLAVILVGADPASQIYVGNKRKSCEEVGILSKSYDLPETTTQNELL
AIIDQLNADKNIDGILVQLPLPKQINAEAIIEHIDPKKDVDGFHPYNVGRLCQRIPTLRACTPYGVMKLLETTGIDLHGK
HAVIVGASNIVGRPMSLELLLAGATVTVTHRFTKNLENHVRQADILVVAVGKPNLISGDWIKESAVVIDVGINRVDGKLV
GDIEFDKAAEKAAYITPVPGGVGPMTVAMLMSNTLYAYEHNK

Sequences:

>Translated_282_residues
MAAKIISGTELSKQIKANLADKITHYIEQGKRVPGLAVILVGADPASQIYVGNKRKSCEEVGILSKSYDLPETTTQNELL
AIIDQLNADKNIDGILVQLPLPKQINAEAIIEHIDPKKDVDGFHPYNVGRLCQRIPTLRACTPYGVMKLLETTGIDLHGK
HAVIVGASNIVGRPMSLELLLAGATVTVTHRFTKNLENHVRQADILVVAVGKPNLISGDWIKESAVVIDVGINRVDGKLV
GDIEFDKAAEKAAYITPVPGGVGPMTVAMLMSNTLYAYEHNK
>Mature_281_residues
AAKIISGTELSKQIKANLADKITHYIEQGKRVPGLAVILVGADPASQIYVGNKRKSCEEVGILSKSYDLPETTTQNELLA
IIDQLNADKNIDGILVQLPLPKQINAEAIIEHIDPKKDVDGFHPYNVGRLCQRIPTLRACTPYGVMKLLETTGIDLHGKH
AVIVGASNIVGRPMSLELLLAGATVTVTHRFTKNLENHVRQADILVVAVGKPNLISGDWIKESAVVIDVGINRVDGKLVG
DIEFDKAAEKAAYITPVPGGVGPMTVAMLMSNTLYAYEHNK

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222418558, Length=291, Percent_Identity=46.7353951890034, Blast_Score=244, Evalue=6e-65,
Organism=Homo sapiens, GI94721354, Length=291, Percent_Identity=47.4226804123711, Blast_Score=243, Evalue=2e-64,
Organism=Homo sapiens, GI222136639, Length=292, Percent_Identity=46.2328767123288, Blast_Score=239, Evalue=1e-63,
Organism=Homo sapiens, GI36796743, Length=213, Percent_Identity=26.7605633802817, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1786741, Length=278, Percent_Identity=66.5467625899281, Blast_Score=386, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17568735, Length=289, Percent_Identity=42.560553633218, Blast_Score=206, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6319558, Length=283, Percent_Identity=47.7031802120141, Blast_Score=241, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6321643, Length=298, Percent_Identity=42.2818791946309, Blast_Score=224, Evalue=9e-60,
Organism=Saccharomyces cerevisiae, GI6322933, Length=316, Percent_Identity=26.5822784810127, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17136818, Length=291, Percent_Identity=48.1099656357388, Blast_Score=250, Evalue=7e-67,
Organism=Drosophila melanogaster, GI17136816, Length=291, Percent_Identity=48.1099656357388, Blast_Score=250, Evalue=9e-67,
Organism=Drosophila melanogaster, GI62472483, Length=286, Percent_Identity=44.4055944055944, Blast_Score=224, Evalue=4e-59,
Organism=Drosophila melanogaster, GI45551871, Length=286, Percent_Identity=44.4055944055944, Blast_Score=224, Evalue=4e-59,
Organism=Drosophila melanogaster, GI24645718, Length=286, Percent_Identity=44.4055944055944, Blast_Score=224, Evalue=5e-59,
Organism=Drosophila melanogaster, GI17137370, Length=286, Percent_Identity=44.4055944055944, Blast_Score=224, Evalue=5e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 30484; Mature: 30353

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKIISGTELSKQIKANLADKITHYIEQGKRVPGLAVILVGADPASQIYVGNKRKSCEE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEECCCCCCHHH
VGILSKSYDLPETTTQNELLAIIDQLNADKNIDGILVQLPLPKQINAEAIIEHIDPKKDV
HCCHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCC
DGFHPYNVGRLCQRIPTLRACTPYGVMKLLETTGIDLHGKHAVIVGASNIVGRPMSLELL
CCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECHHHHCCCCEEEEE
LAGATVTVTHRFTKNLENHVRQADILVVAVGKPNLISGDWIKESAVVIDVGINRVDGKLV
EECCEEEEEHHHHHHHHHHHCEEEEEEEECCCCCEECCCCCCCCEEEEEECCCEECCEEE
GDIEFDKAAEKAAYITPVPGGVGPMTVAMLMSNTLYAYEHNK
ECCCHHHHHCCCEEEECCCCCCHHHHHHHHHHCEEEEEECCC
>Mature Secondary Structure 
AAKIISGTELSKQIKANLADKITHYIEQGKRVPGLAVILVGADPASQIYVGNKRKSCEE
CCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEECCCCCCHHH
VGILSKSYDLPETTTQNELLAIIDQLNADKNIDGILVQLPLPKQINAEAIIEHIDPKKDV
HCCHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHCCCCCCC
DGFHPYNVGRLCQRIPTLRACTPYGVMKLLETTGIDLHGKHAVIVGASNIVGRPMSLELL
CCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECHHHHCCCCEEEEE
LAGATVTVTHRFTKNLENHVRQADILVVAVGKPNLISGDWIKESAVVIDVGINRVDGKLV
EECCEEEEEHHHHHHHHHHHCEEEEEEEECCCCCEECCCCCCCCEEEEEECCCEECCEEE
GDIEFDKAAEKAAYITPVPGGVGPMTVAMLMSNTLYAYEHNK
ECCCHHHHHCCCEEEECCCCCCHHHHHHHHHHCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA