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The map label for this gene is rluE [H]

Identifier: 148827867

GI number: 148827867

Start: 1233229

End: 1233912

Strand: Direct

Name: rluE [H]

Synonym: CGSHiGG_06765

Alternate gene names: 148827867

Gene position: 1233229-1233912 (Clockwise)

Preceding gene: 148827866

Following gene: 148827873

Centisome position: 65.35

GC content: 38.01

Gene sequence:

>684_bases
ATGAAGAAAAATTTTTCAGCAGGGAAATTGCCGTCTAAAGGAAAAAGTGCGGTTAATTTTCACCGCACTTTTAAGCCAAA
ATTAAAACCCAAAACGTTGTCGTTGGATGAAACGAAAGTTGTGTTATTCAATAAACCTTTTGATGTGCTCACGCAATTTA
CCGACGAACAAGGACGCGCTACCTTAAAAGATTTTATTTCAATTCCAAATGTTTATGCGGCAGGTCGATTAGATAGAGAC
AGTGAAGGATTACTGATTTTAACCAATAATGGCGAATTACAACATCGTTTGGCAGATCCCAAATTTAAAACAGAAAAAAC
CTATTGGGTGCAAGTGGAAGGTATCCCTGAAGAAACCGATTTAGCACAACTGAGAAAAGGTGTAGAACTTAAAGATGGCG
TGACTAAACCCGCTAAAGTGCGGTTAATTTCAGAGCCAAATTTATGGGAACGCAATCCACCTATCCGAGAACGGAAAAAT
ATTCCCACAAGCTGGTTAGAAATTAAGATTAGTGAAGGGCGTAACCGCCAAGTGCGGAGAATGACTGCGCATATTGGTTT
CCCCACTTTACGATTAGTGAGAGTGAGTATGGGCTCATTATCTATTAATGGATTAGAAAATGGCTCATTTAGATTATTAT
CATTGGATGAAATAAAAGCGTTATTTCAGACCGTCAAATTATAA

Upstream 100 bases:

>100_bases
GCCTTTTTAATTTTTCAAGTTCACTCAAGTAATCATCATTACTACAGCCTGAAGTTTTGCTATGATGTTGCAAGCCTTTC
AAAATAGAAATCATAAGTTA

Downstream 100 bases:

>100_bases
AAATAGGGCAAGTATTTAGCTTGCCCTAAATTTTTCTCCCATAAATATTGCGCACTGAGTAAATTAGCACTATCCACAAC
GCGATGAGCTTGGTCAATAT

Product: hypothetical protein

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MKKNFSAGKLPSKGKSAVNFHRTFKPKLKPKTLSLDETKVVLFNKPFDVLTQFTDEQGRATLKDFISIPNVYAAGRLDRD
SEGLLILTNNGELQHRLADPKFKTEKTYWVQVEGIPEETDLAQLRKGVELKDGVTKPAKVRLISEPNLWERNPPIRERKN
IPTSWLEIKISEGRNRQVRRMTAHIGFPTLRLVRVSMGSLSINGLENGSFRLLSLDEIKALFQTVKL

Sequences:

>Translated_227_residues
MKKNFSAGKLPSKGKSAVNFHRTFKPKLKPKTLSLDETKVVLFNKPFDVLTQFTDEQGRATLKDFISIPNVYAAGRLDRD
SEGLLILTNNGELQHRLADPKFKTEKTYWVQVEGIPEETDLAQLRKGVELKDGVTKPAKVRLISEPNLWERNPPIRERKN
IPTSWLEIKISEGRNRQVRRMTAHIGFPTLRLVRVSMGSLSINGLENGSFRLLSLDEIKALFQTVKL
>Mature_227_residues
MKKNFSAGKLPSKGKSAVNFHRTFKPKLKPKTLSLDETKVVLFNKPFDVLTQFTDEQGRATLKDFISIPNVYAAGRLDRD
SEGLLILTNNGELQHRLADPKFKTEKTYWVQVEGIPEETDLAQLRKGVELKDGVTKPAKVRLISEPNLWERNPPIRERKN
IPTSWLEIKISEGRNRQVRRMTAHIGFPTLRLVRVSMGSLSINGLENGSFRLLSLDEIKALFQTVKL

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=175, Percent_Identity=66.8571428571429, Blast_Score=241, Evalue=2e-65,
Organism=Escherichia coli, GI1787524, Length=239, Percent_Identity=30.5439330543933, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1788510, Length=189, Percent_Identity=31.7460317460317, Blast_Score=88, Evalue=4e-19,
Organism=Escherichia coli, GI1790453, Length=188, Percent_Identity=28.7234042553192, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 25835; Mature: 25835

Theoretical pI: Translated: 10.77; Mature: 10.77

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNFSAGKLPSKGKSAVNFHRTFKPKLKPKTLSLDETKVVLFNKPFDVLTQFTDEQGRA
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEECCCCEEEEEEECCHHHHHHHCCCCCHH
TLKDFISIPNVYAAGRLDRDSEGLLILTNNGELQHRLADPKFKTEKTYWVQVEGIPEETD
HHHHHHCCCCEEECCCCCCCCCCEEEEECCCCEEEECCCCCCCCCCEEEEEEECCCCCCC
LAQLRKGVELKDGVTKPAKVRLISEPNLWERNPPIRERKNIPTSWLEIKISEGRNRQVRR
HHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHCCCCCEEEEEEECCCCCHHHHH
MTAHIGFPTLRLVRVSMGSLSINGLENGSFRLLSLDEIKALFQTVKL
HHHHCCCCCEEEEEEECCCEEECCCCCCCEEEEEHHHHHHHHHHHCC
>Mature Secondary Structure
MKKNFSAGKLPSKGKSAVNFHRTFKPKLKPKTLSLDETKVVLFNKPFDVLTQFTDEQGRA
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCCEECCCCEEEEEEECCHHHHHHHCCCCCHH
TLKDFISIPNVYAAGRLDRDSEGLLILTNNGELQHRLADPKFKTEKTYWVQVEGIPEETD
HHHHHHCCCCEEECCCCCCCCCCEEEEECCCCEEEECCCCCCCCCCEEEEEEECCCCCCC
LAQLRKGVELKDGVTKPAKVRLISEPNLWERNPPIRERKNIPTSWLEIKISEGRNRQVRR
HHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCHHHHCCCCCEEEEEEECCCCCHHHHH
MTAHIGFPTLRLVRVSMGSLSINGLENGSFRLLSLDEIKALFQTVKL
HHHHCCCCCEEEEEEECCCEEECCCCCCCEEEEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]