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The map label for this gene is potE [H]

Identifier: 148827797

GI number: 148827797

Start: 1151559

End: 1152875

Strand: Direct

Name: potE [H]

Synonym: CGSHiGG_06330

Alternate gene names: 148827797

Gene position: 1151559-1152875 (Clockwise)

Preceding gene: 148827796

Following gene: 148827800

Centisome position: 61.02

GC content: 39.33

Gene sequence:

>1317_bases
ATGAGCGCTAAAAGTAATAAAATTGGTGTGGTACAACTTACCATTCTTACCATGGTTAATATGATGGGTTCCGGTATCAT
TATGTTACCGACCAAATTAGCTGAAATTGGGACTATTTCCATCGTTTCTTGGCTTGTAACAGCCGTTGGTTCAACAGCCT
TAGCTTACGCATTCGCACAATGCGGTATGTTCAGTAAAAAATCTGGTGGTATGGGCGGTTATGCGGAATACTCTTTCGGT
AAAGCCGGTAACTTCATGGCTAACTACACTTACGGTGTATCATTAGTTATTGCGAATACAGCAATCGCGATTTCTGCGGT
GGGTTATGGTTCAGAGCTTCTCGGCGCAACCCTTTCTCCACTTTCTATCGCATTATGGACCATTTTCACCCTTTGGCTTG
CAACCGTTCTTAACTTTGGTGGTGCAAGAATCACCGGTAATATAAGTTCATTTACTATCTGGGGCGTAATTATCCCTGTA
GTAGGTATTTCTATCATTGGTTGGAAATGGTTTGATGGTTCAATGTATGTAAACTCTTGGAACCCACATAATGTACCAAC
TTTTGAAGCGATCGGTGTATCTATTTCAATGACATTATGGGCGTTCTTAGGGTTAGAATCAGCTTGTGCTAACGCAGATG
CAGTTGAAAACCCTGAGAAAAACGTACCAATAGCAGTACTTGGTGGTACTTTAGGGGCAGCGGTAATTTATATTGTTTCA
ACTAACGTGATTGCAGGTATTGTACCTAACCTTGAACTCGCTAATTCAACAGCGCCATTCGGTTTAGCCTTTGCTCATAT
GTTTGATGAAATTGTCGGTAAAGTTATCATGGTCTTAATGGTGATGTCTTGCTTTGGTTCATTATTAGGCTGGCAGTTCA
CTATTGCTCAAGTATTCAAATCTTCAGCAGAAGAAGGTTATTTCCCAGCATTCTTCAAAAAAGTTACCACTAAAGATGCA
CCTGTTGTTGGTATGATTACCATTACTGCGTTACAAACTTTACTCTCTTTAATGACAATCAGCCCATCATTAAATAAACA
ATTCAATGTGTTGGTGGACTTAGCGGTAGTAACAAACGTTATTCCATACTTGCTCTCAATGGCTGCACTTGCAGTGTTAT
TGAAAGCAGAGAATGTTACGCCACAAAAATATAAAACAACCGTTTTTGTTGCCTTTATTGGTTCACTATATAGTATCTAT
GCGCTTTATGCAGCCGGTGAACAAGCGATGTTATATGGTTCAATTGTGACCTTTATCGGTTGGACATTGTATGGTTTTGT
TTCTTACAAATTTGACCTTAAAAAATCTCAAGCTTAA

Upstream 100 bases:

>100_bases
GAGGTTGTGAGGATCTTCCTCATAACCCTCTCAGCACAAACAAAAGAAAGTGCGGTTAAAAACATCTGATTTTTTGACCG
CACTTGTAGGAGTATAAATT

Downstream 100 bases:

>100_bases
TTAAAAAAAGAAAAGCCTACATCTTAATGTAGGCTCTTTCGAGTAGTAATAAGCTAAGTACAAAAGAGCGGTTGAAAATT
TAATTGTTTTCAACCGTTCT

Product: putrescine transporter

Products: Proton [Cytoplasm]; putrescine [Cytoplasm] [C]

Alternate protein names: Putrescine transport protein [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MSAKSNKIGVVQLTILTMVNMMGSGIIMLPTKLAEIGTISIVSWLVTAVGSTALAYAFAQCGMFSKKSGGMGGYAEYSFG
KAGNFMANYTYGVSLVIANTAIAISAVGYGSELLGATLSPLSIALWTIFTLWLATVLNFGGARITGNISSFTIWGVIIPV
VGISIIGWKWFDGSMYVNSWNPHNVPTFEAIGVSISMTLWAFLGLESACANADAVENPEKNVPIAVLGGTLGAAVIYIVS
TNVIAGIVPNLELANSTAPFGLAFAHMFDEIVGKVIMVLMVMSCFGSLLGWQFTIAQVFKSSAEEGYFPAFFKKVTTKDA
PVVGMITITALQTLLSLMTISPSLNKQFNVLVDLAVVTNVIPYLLSMAALAVLLKAENVTPQKYKTTVFVAFIGSLYSIY
ALYAAGEQAMLYGSIVTFIGWTLYGFVSYKFDLKKSQA

Sequences:

>Translated_438_residues
MSAKSNKIGVVQLTILTMVNMMGSGIIMLPTKLAEIGTISIVSWLVTAVGSTALAYAFAQCGMFSKKSGGMGGYAEYSFG
KAGNFMANYTYGVSLVIANTAIAISAVGYGSELLGATLSPLSIALWTIFTLWLATVLNFGGARITGNISSFTIWGVIIPV
VGISIIGWKWFDGSMYVNSWNPHNVPTFEAIGVSISMTLWAFLGLESACANADAVENPEKNVPIAVLGGTLGAAVIYIVS
TNVIAGIVPNLELANSTAPFGLAFAHMFDEIVGKVIMVLMVMSCFGSLLGWQFTIAQVFKSSAEEGYFPAFFKKVTTKDA
PVVGMITITALQTLLSLMTISPSLNKQFNVLVDLAVVTNVIPYLLSMAALAVLLKAENVTPQKYKTTVFVAFIGSLYSIY
ALYAAGEQAMLYGSIVTFIGWTLYGFVSYKFDLKKSQA
>Mature_437_residues
SAKSNKIGVVQLTILTMVNMMGSGIIMLPTKLAEIGTISIVSWLVTAVGSTALAYAFAQCGMFSKKSGGMGGYAEYSFGK
AGNFMANYTYGVSLVIANTAIAISAVGYGSELLGATLSPLSIALWTIFTLWLATVLNFGGARITGNISSFTIWGVIIPVV
GISIIGWKWFDGSMYVNSWNPHNVPTFEAIGVSISMTLWAFLGLESACANADAVENPEKNVPIAVLGGTLGAAVIYIVST
NVIAGIVPNLELANSTAPFGLAFAHMFDEIVGKVIMVLMVMSCFGSLLGWQFTIAQVFKSSAEEGYFPAFFKKVTTKDAP
VVGMITITALQTLLSLMTISPSLNKQFNVLVDLAVVTNVIPYLLSMAALAVLLKAENVTPQKYKTTVFVAFIGSLYSIYA
LYAAGEQAMLYGSIVTFIGWTLYGFVSYKFDLKKSQA

Specific function: Probable putrescine-ornithine antiporter [H]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Escherichia coli, GI1786908, Length=436, Percent_Identity=77.5229357798165, Blast_Score=688, Evalue=0.0,
Organism=Escherichia coli, GI2367353, Length=418, Percent_Identity=34.4497607655502, Blast_Score=258, Evalue=6e-70,
Organism=Escherichia coli, GI1790575, Length=441, Percent_Identity=29.7052154195011, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1787890, Length=425, Percent_Identity=24.9411764705882, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1786602, Length=351, Percent_Identity=21.9373219373219, Blast_Score=65, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17540018, Length=327, Percent_Identity=26.2996941896024, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI45550968, Length=330, Percent_Identity=24.5454545454545, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19921172, Length=330, Percent_Identity=24.5454545454545, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004754 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 46643; Mature: 46512

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKSNKIGVVQLTILTMVNMMGSGIIMLPTKLAEIGTISIVSWLVTAVGSTALAYAFAQ
CCCCCCCCEEEEHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CGMFSKKSGGMGGYAEYSFGKAGNFMANYTYGVSLVIANTAIAISAVGYGSELLGATLSP
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCEEEEEEHHEEEEEECCCCHHHHHCCCCH
LSIALWTIFTLWLATVLNFGGARITGNISSFTIWGVIIPVVGISIIGWKWFDGSMYVNSW
HHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHEEEEEECCEEEEECC
NPHNVPTFEAIGVSISMTLWAFLGLESACANADAVENPEKNVPIAVLGGTLGAAVIYIVS
CCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHEE
TNVIAGIVPNLELANSTAPFGLAFAHMFDEIVGKVIMVLMVMSCFGSLLGWQFTIAQVFK
HHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSAEEGYFPAFFKKVTTKDAPVVGMITITALQTLLSLMTISPSLNKQFNVLVDLAVVTNV
CCCCCCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
IPYLLSMAALAVLLKAENVTPQKYKTTVFVAFIGSLYSIYALYAAGEQAMLYGSIVTFIG
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
WTLYGFVSYKFDLKKSQA
HHHHHHHHEEEECCCCCC
>Mature Secondary Structure 
SAKSNKIGVVQLTILTMVNMMGSGIIMLPTKLAEIGTISIVSWLVTAVGSTALAYAFAQ
CCCCCCCEEEEHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CGMFSKKSGGMGGYAEYSFGKAGNFMANYTYGVSLVIANTAIAISAVGYGSELLGATLSP
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCEEEEEEHHEEEEEECCCCHHHHHCCCCH
LSIALWTIFTLWLATVLNFGGARITGNISSFTIWGVIIPVVGISIIGWKWFDGSMYVNSW
HHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHEEEEEECCEEEEECC
NPHNVPTFEAIGVSISMTLWAFLGLESACANADAVENPEKNVPIAVLGGTLGAAVIYIVS
CCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHEE
TNVIAGIVPNLELANSTAPFGLAFAHMFDEIVGKVIMVLMVMSCFGSLLGWQFTIAQVFK
HHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSAEEGYFPAFFKKVTTKDAPVVGMITITALQTLLSLMTISPSLNKQFNVLVDLAVVTNV
CCCCCCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
IPYLLSMAALAVLLKAENVTPQKYKTTVFVAFIGSLYSIYALYAAGEQAMLYGSIVTFIG
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
WTLYGFVSYKFDLKKSQA
HHHHHHHHEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; putrescine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + putrescine [Periplasm] = Proton [Cytoplasm] + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]