Click here to switch to the map view.

The map label for this gene is ligA

Identifier: 148824204

GI number: 148824204

Start: 3384254

End: 3386329

Strand: Reverse

Name: ligA

Synonym: TBFG_13029

Alternate gene names: 148824204

Gene position: 3386329-3384254 (Counterclockwise)

Preceding gene: 148824205

Following gene: 148824202

Centisome position: 76.54

GC content: 69.12

Gene sequence:

>2076_bases
GTGAGCTCCCCAGACGCCGATCAGACCGCTCCCGAGGTGTTGCGGCAGTGGCAGGCACTGGCCGAGGAGGTGCGTGAGCA
CCAGTTCCGTTATTACGTGCGGGACGCGCCGATCATCAGCGACGCGGAATTCGACGAGCTGCTGCGCCGTCTGGAAGCCC
TCGAGGAGCAGCATCCCGAGCTGCGCACGCCCGATTCGCCGACCCAGCTGGTCGGCGGTGCCGGCTTCGCCACGGATTTC
GAGCCCGTCGACCATCTCGAACGAATGCTCAGCCTCGACAACGCGTTCACCGCCGACGAACTCGCCGCCTGGGCCGGCCG
CATCCATGCCGAGGTCGGAGACGCCGCACATTACCTGTGTGAGCTCAAGATCGACGGCGTCGCGCTGTCTTTGGTCTACC
GCGAGGGACGGCTGACCCGGGCCTCCACCCGCGGCGACGGGCGCACCGGCGAGGACGTCACCCTGAACGCCCGGACCATC
GCCGACGTTCCCGAACGGCTCACCCCCGGCGACGACTACCCGGTGCCCGAGGTCCTCGAGGTCCGCGGCGAGGTCTTCTT
CCGGCTGGACGACTTCCAGGCGCTCAACGCCAGCCTCGTCGAGGAGGGCAAGGCGCCGTTCGCCAACCCCCGCAACAGCG
CGGCGGGATCGCTGCGCCAGAAAGACCCGGCGGTCACCGCGCGCCGCCGGCTGCGGATGATCTGCCACGGGCTGGGCCAC
GTGGAGGGCTTTCGCCCGGCCACCCTGCATCAGGCATACCTGGCGTTGCGGGCATGGGGACTGCCGGTTTCCGAACACAC
CACCCTGGCAACCGACCTGGCCGGTGTGCGCGAGCGCATCGACTACTGGGGCGAGCACCGCCACGAGGTGGACCACGAAA
TCGACGGCGTGGTGGTCAAAGTCGACGAGGTGGCGTTGCAGCGCAGGCTGGGTTCCACGTCGCGGGCGCCGCGCTGGGCC
ATCGCCTACAAGTACCCGCCCGAGGAAGCGCAGACCAAGCTGCTCGACATCCGGGTGAACGTCGGCCGCACCGGGCGGAT
CACGCCGTTTGCGTTCATGACGCCGGTGAAGGTGGCCGGGTCGACGGTGGGACAGGCCACCCTGCACAACGCCTCGGAGA
TCAAGCGCAAGGGCGTGCTGATCGGCGACACCGTGGTGATCCGCAAGGCCGGCGACGTGATCCCCGAGGTGCTGGGACCC
GTCGTCGAACTGCGCGATGGCTCCGAACGCGAATTCATCATGCCCACCACCTGCCCGGAGTGCGGTTCGCCGTTGGCGCC
GGAGAAGGAAGGCGACGCCGACATCCGTTGCCCCAACGCCCGCGGCTGCCCGGGGCAACTGCGGGAGCGGGTTTTCCACG
TCGCCAGCCGCAACGGCCTAGACATCGAGGTGCTCGGTTACGAGGCGGGTGTGGCGCTCTTGCAGGCGAAGGTGATCGCC
GACGAGGGCGAGCTGTTCGCGCTGACCGAGCGGGACTTGCTGCGCACCGACCTGTTCCGAACCAAGGCAGGCGAACTGTC
GGCCAACGGCAAACGGCTGCTGGTCAACCTCGACAAGGCCAAGGCGGCACCGCTGTGGCGGGTGCTGGTGGCGCTGTCCA
TCCGCCATGTCGGGCCGACGGCGGCCCGCGCCCTGGCCACCGAGTTCGGCAGCCTTGACGCCATCGCCGCGGCGTCCACC
GACCAGCTGGCCGCCGTCGAGGGGGTGGGGCCGACCATTGCCGCCGCGGTCACCGAGTGGTTCGCCGTCGACTGGCACCG
CGAGATCGTCGACAAGTGGCGGGCCGCCGGGGTGCGAATGGTCGACGAGCGTGACGAGAGTGTGCCACGCACGCTGGCCG
GGCTGACCATCGTGGTCACCGGCTCGCTGACCGGTTTCTCCCGCGACGACGCCAAGGAGGCGATCGTGGCCCGCGGCGGC
AAGGCCGCCGGCTCGGTGTCGAAGAAGACCAACTATGTCGTCGCCGGAGACTCGCCGGGATCCAAATACGACAAGGCGGT
GGAGTTGGGGGTGCCGATTCTGGACGAGGATGGGTTCCGGAGACTGCTGGCCGACGGACCCGCGTCACGAACGTAA

Upstream 100 bases:

>100_bases
GGCCCGCACCGCGGTCGGGCTTGCCCGTGATGTCGCTGAGGCGTTCGCGCGGGACCCAGAGGCCATCTGAGGGAAGAGCC
CCCTCGGCTAGCCTGCCAGA

Downstream 100 bases:

>100_bases
CGGCACTGCGAATCTCCGCGCGGTTTTTCGCAGTGGCGTTACGCTCGGCGAACCTTGGTTGCCAACGAACCGCTCAGCGC
AGCATCGTCGCCACGATGCC

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 691; Mature: 690

Protein sequence:

>691_residues
MSSPDADQTAPEVLRQWQALAEEVREHQFRYYVRDAPIISDAEFDELLRRLEALEEQHPELRTPDSPTQLVGGAGFATDF
EPVDHLERMLSLDNAFTADELAAWAGRIHAEVGDAAHYLCELKIDGVALSLVYREGRLTRASTRGDGRTGEDVTLNARTI
ADVPERLTPGDDYPVPEVLEVRGEVFFRLDDFQALNASLVEEGKAPFANPRNSAAGSLRQKDPAVTARRRLRMICHGLGH
VEGFRPATLHQAYLALRAWGLPVSEHTTLATDLAGVRERIDYWGEHRHEVDHEIDGVVVKVDEVALQRRLGSTSRAPRWA
IAYKYPPEEAQTKLLDIRVNVGRTGRITPFAFMTPVKVAGSTVGQATLHNASEIKRKGVLIGDTVVIRKAGDVIPEVLGP
VVELRDGSEREFIMPTTCPECGSPLAPEKEGDADIRCPNARGCPGQLRERVFHVASRNGLDIEVLGYEAGVALLQAKVIA
DEGELFALTERDLLRTDLFRTKAGELSANGKRLLVNLDKAKAAPLWRVLVALSIRHVGPTAARALATEFGSLDAIAAAST
DQLAAVEGVGPTIAAAVTEWFAVDWHREIVDKWRAAGVRMVDERDESVPRTLAGLTIVVTGSLTGFSRDDAKEAIVARGG
KAAGSVSKKTNYVVAGDSPGSKYDKAVELGVPILDEDGFRRLLADGPASRT

Sequences:

>Translated_691_residues
MSSPDADQTAPEVLRQWQALAEEVREHQFRYYVRDAPIISDAEFDELLRRLEALEEQHPELRTPDSPTQLVGGAGFATDF
EPVDHLERMLSLDNAFTADELAAWAGRIHAEVGDAAHYLCELKIDGVALSLVYREGRLTRASTRGDGRTGEDVTLNARTI
ADVPERLTPGDDYPVPEVLEVRGEVFFRLDDFQALNASLVEEGKAPFANPRNSAAGSLRQKDPAVTARRRLRMICHGLGH
VEGFRPATLHQAYLALRAWGLPVSEHTTLATDLAGVRERIDYWGEHRHEVDHEIDGVVVKVDEVALQRRLGSTSRAPRWA
IAYKYPPEEAQTKLLDIRVNVGRTGRITPFAFMTPVKVAGSTVGQATLHNASEIKRKGVLIGDTVVIRKAGDVIPEVLGP
VVELRDGSEREFIMPTTCPECGSPLAPEKEGDADIRCPNARGCPGQLRERVFHVASRNGLDIEVLGYEAGVALLQAKVIA
DEGELFALTERDLLRTDLFRTKAGELSANGKRLLVNLDKAKAAPLWRVLVALSIRHVGPTAARALATEFGSLDAIAAAST
DQLAAVEGVGPTIAAAVTEWFAVDWHREIVDKWRAAGVRMVDERDESVPRTLAGLTIVVTGSLTGFSRDDAKEAIVARGG
KAAGSVSKKTNYVVAGDSPGSKYDKAVELGVPILDEDGFRRLLADGPASRT
>Mature_690_residues
SSPDADQTAPEVLRQWQALAEEVREHQFRYYVRDAPIISDAEFDELLRRLEALEEQHPELRTPDSPTQLVGGAGFATDFE
PVDHLERMLSLDNAFTADELAAWAGRIHAEVGDAAHYLCELKIDGVALSLVYREGRLTRASTRGDGRTGEDVTLNARTIA
DVPERLTPGDDYPVPEVLEVRGEVFFRLDDFQALNASLVEEGKAPFANPRNSAAGSLRQKDPAVTARRRLRMICHGLGHV
EGFRPATLHQAYLALRAWGLPVSEHTTLATDLAGVRERIDYWGEHRHEVDHEIDGVVVKVDEVALQRRLGSTSRAPRWAI
AYKYPPEEAQTKLLDIRVNVGRTGRITPFAFMTPVKVAGSTVGQATLHNASEIKRKGVLIGDTVVIRKAGDVIPEVLGPV
VELRDGSEREFIMPTTCPECGSPLAPEKEGDADIRCPNARGCPGQLRERVFHVASRNGLDIEVLGYEAGVALLQAKVIAD
EGELFALTERDLLRTDLFRTKAGELSANGKRLLVNLDKAKAAPLWRVLVALSIRHVGPTAARALATEFGSLDAIAAASTD
QLAAVEGVGPTIAAAVTEWFAVDWHREIVDKWRAAGVRMVDERDESVPRTLAGLTIVVTGSLTGFSRDDAKEAIVARGGK
AAGSVSKKTNYVVAGDSPGSKYDKAVELGVPILDEDGFRRLLADGPASRT

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=81, Percent_Identity=50.6172839506173, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788750, Length=675, Percent_Identity=43.5555555555556, Blast_Score=499, Evalue=1e-142,
Organism=Escherichia coli, GI87082305, Length=465, Percent_Identity=23.6559139784946, Blast_Score=80, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_MYCBO (P63974)

Other databases:

- EMBL:   BX248344
- RefSeq:   NP_856684.1
- ProteinModelPortal:   P63974
- EnsemblBacteria:   EBMYCT00000014995
- GeneID:   1093559
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb3039c
- GeneTree:   EBGT00050000016652
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   PRK07956
- BioCyc:   MBOV233413:MB3039C-MONOMER
- BRENDA:   6.5.1.2
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; PF00633 HHH; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 75290; Mature: 75159

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 123-123 BINDING 121-121 BINDING 144-144 BINDING 184-184 BINDING 300-300 BINDING 324-324

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSPDADQTAPEVLRQWQALAEEVREHQFRYYVRDAPIISDAEFDELLRRLEALEEQHPE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHHCCCC
LRTPDSPTQLVGGAGFATDFEPVDHLERMLSLDNAFTADELAAWAGRIHAEVGDAAHYLC
CCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEE
ELKIDGVALSLVYREGRLTRASTRGDGRTGEDVTLNARTIADVPERLTPGDDYPVPEVLE
EEEECCEEEEEEEECCCEEECCCCCCCCCCCCEEEECHHHHHCHHHCCCCCCCCCHHHHH
VRGEVFFRLDDFQALNASLVEEGKAPFANPRNSAAGSLRQKDPAVTARRRLRMICHGLGH
CCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHCC
VEGFRPATLHQAYLALRAWGLPVSEHTTLATDLAGVRERIDYWGEHRHEVDHEIDGVVVK
CCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
VDEVALQRRLGSTSRAPRWAIAYKYPPEEAQTKLLDIRVNVGRTGRITPFAFMTPVKVAG
HHHHHHHHHCCCCCCCCCEEEEEECCCHHHHCEEEEEEEECCCCCCCCCCHHHCCHHHHC
STVGQATLHNASEIKRKGVLIGDTVVIRKAGDVIPEVLGPVVELRDGSEREFIMPTTCPE
CCCCHHHHCCHHHHHHCCEEECCEEEEECCHHHHHHHHHHHHCCCCCCCCCEECCCCCCC
CGSPLAPEKEGDADIRCPNARGCPGQLRERVFHVASRNGLDIEVLGYEAGVALLQAKVIA
CCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHEE
DEGELFALTERDLLRTDLFRTKAGELSANGKRLLVNLDKAKAAPLWRVLVALSIRHVGPT
CCCCEEEECHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHCCHHHHHHHHHHHHHCCHH
AARALATEFGSLDAIAAASTDQLAAVEGVGPTIAAAVTEWFAVDWHREIVDKWRAAGVRM
HHHHHHHHHCCCHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
VDERDESVPRTLAGLTIVVTGSLTGFSRDDAKEAIVARGGKAAGSVSKKTNYVVAGDSPG
ECCCCCHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEECCCCC
SKYDKAVELGVPILDEDGFRRLLADGPASRT
CHHHHHHHCCCCEECCCHHHHHHHCCCCCCC
>Mature Secondary Structure 
SSPDADQTAPEVLRQWQALAEEVREHQFRYYVRDAPIISDAEFDELLRRLEALEEQHPE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHHHHHHHHHHCCCC
LRTPDSPTQLVGGAGFATDFEPVDHLERMLSLDNAFTADELAAWAGRIHAEVGDAAHYLC
CCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEE
ELKIDGVALSLVYREGRLTRASTRGDGRTGEDVTLNARTIADVPERLTPGDDYPVPEVLE
EEEECCEEEEEEEECCCEEECCCCCCCCCCCCEEEECHHHHHCHHHCCCCCCCCCHHHHH
VRGEVFFRLDDFQALNASLVEEGKAPFANPRNSAAGSLRQKDPAVTARRRLRMICHGLGH
CCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHCC
VEGFRPATLHQAYLALRAWGLPVSEHTTLATDLAGVRERIDYWGEHRHEVDHEIDGVVVK
CCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
VDEVALQRRLGSTSRAPRWAIAYKYPPEEAQTKLLDIRVNVGRTGRITPFAFMTPVKVAG
HHHHHHHHHCCCCCCCCCEEEEEECCCHHHHCEEEEEEEECCCCCCCCCCHHHCCHHHHC
STVGQATLHNASEIKRKGVLIGDTVVIRKAGDVIPEVLGPVVELRDGSEREFIMPTTCPE
CCCCHHHHCCHHHHHHCCEEECCEEEEECCHHHHHHHHHHHHCCCCCCCCCEECCCCCCC
CGSPLAPEKEGDADIRCPNARGCPGQLRERVFHVASRNGLDIEVLGYEAGVALLQAKVIA
CCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHEE
DEGELFALTERDLLRTDLFRTKAGELSANGKRLLVNLDKAKAAPLWRVLVALSIRHVGPT
CCCCEEEECHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHCCHHHHHHHHHHHHHCCHH
AARALATEFGSLDAIAAASTDQLAAVEGVGPTIAAAVTEWFAVDWHREIVDKWRAAGVRM
HHHHHHHHHCCCHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
VDERDESVPRTLAGLTIVVTGSLTGFSRDDAKEAIVARGGKAAGSVSKKTNYVVAGDSPG
ECCCCCHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEECCCCC
SKYDKAVELGVPILDEDGFRRLLADGPASRT
CHHHHHHHCCCCEECCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972