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The map label for this gene is leuC [H]

Identifier: 148824178

GI number: 148824178

Start: 3356363

End: 3357784

Strand: Reverse

Name: leuC [H]

Synonym: TBFG_13003

Alternate gene names: 148824178

Gene position: 3357784-3356363 (Counterclockwise)

Preceding gene: 148824180

Following gene: 148824177

Centisome position: 75.89

GC content: 66.6

Gene sequence:

>1422_bases
ATGGCCTTGCAGACCGGCGAGCCACGCACACTGGCCGAGAAGATTTGGGACGACCACATTGTGGTATCAGGCGGGGGCTG
TGCGCCCGACTTGATCTACATCGATCTGCATCTGGTGCATGAGGTCACCAGCCCGCAGGCGTTCGACGGCCTGCGCCTGG
CCGGGCGCCGGGTGCGGCGGCCCGAGCTCACCCTCGCCACCGAGGATCACAACGTGCCCACCGTCGACATCGACCAGCCG
ATCGCCGACCCGGTGTCACGCACCCAGGTGGAGACGTTGCGCCGAAACTGCGCCGAATTCGGAATCCGGCTGCATTCAAT
GGGCGATATCGAGCAGGGCATCGTGCACGTCGTCGGACCGCAATTGGGTCTCACCCAGCCGGGAATGACGATCGTCTGTG
GCGATAGCCACACCTCAACCCACGGCGCATTCGGCGCGTTGGCGATGGGCATCGGTACGTCGGAGGTCGAGCACGTGCTG
GCCACCCAGACGCTGCCGCTGCGGCCGTTCAAGACCATGGCGGTCAACGTCGATGGGCGGCTGCCCGACGGTGTGTCGGC
CAAGGACATCATCCTGGCGTTGATCGCCAAGATCGGCACCGGCGGCGGCCAGGGGCATGTCATCGAATACCGGGGCAGCG
CCATCGAATCGCTGTCCATGGAAGGCCGGATGACGATCTGCAACATGAGCATCGAAGCCGGCGCTCGTGCGGGCATGGTG
GCTCCGGACGAAACCACCTACGCGTTCTTGCGTGGTCGTCCGCACGCACCCACCGGTGCCCAGTGGGACACCGCACTCGT
CTACTGGCAACGGCTTCGCACCGACGTCGGCGCCGTTTTCGACACCGAGGTGTATCTCGACGCCGCGTCGTTGAGCCCGT
TTGTCACCTGGGGCACCAACCCAGGCCAAGGGGTACCGCTGGCGGCCGCGGTGCCAGATCCGCAACTGATGACCGACGAC
GCCGAGCGGCAGGCCGCCGAGAAAGCATTGGCGTACATGGACCTTCGACCCGGAACCGCGATGCGCGACATCGCGGTCGA
CGCCGTGTTCGTCGGGTCGTGTACCAACGGTCGCATTGAAGATCTGCGGGTGGTCGCCGAAGTGCTGCGTGGCCGCAAGG
TGGCCGACGGCGTGCGGATGCTGATCGTTCCGGGCTCAATGCGGGTACGCGCGCAGGCCGAGGCCGAAGGGCTCGGTGAG
ATCTTCACCGACGCGGGCGCGCAATGGCGGCAGGCGGGATGCTCGATGTGTCTGGGCATGAATCCCGATCAGCTGGCGTC
CGGGGAGCGCTGCGCCGCGACGTCCAACCGCAACTTCGAAGGGCGGCAGGGCGCGGGCGGCCGCACACATTTGGTGTCCC
CAGCAGTGGCGGCCGCCACCGCGGTTCGCGGCACACTGTCCAGCCCGGCCGATTTGAATTGA

Upstream 100 bases:

>100_bases
AGGACACCGATACCGCTATGCTGTCTCACAAAGAGATCATAGCGTCTCGTATTTTGAGATGACCAGCGAGTCGCGAACGA
ATGAATAGAGGCACTTCGAG

Downstream 100 bases:

>100_bases
ACTGAGAACAGCTAGGGGATCAGCATGGAAGCCTTTCACACCCACTCTGGTATTGGCGTGCCGTTGCGGCGGTCCAATGT
CGACACCGATCAGATCATTC

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MALQTGEPRTLAEKIWDDHIVVSGGGCAPDLIYIDLHLVHEVTSPQAFDGLRLAGRRVRRPELTLATEDHNVPTVDIDQP
IADPVSRTQVETLRRNCAEFGIRLHSMGDIEQGIVHVVGPQLGLTQPGMTIVCGDSHTSTHGAFGALAMGIGTSEVEHVL
ATQTLPLRPFKTMAVNVDGRLPDGVSAKDIILALIAKIGTGGGQGHVIEYRGSAIESLSMEGRMTICNMSIEAGARAGMV
APDETTYAFLRGRPHAPTGAQWDTALVYWQRLRTDVGAVFDTEVYLDAASLSPFVTWGTNPGQGVPLAAAVPDPQLMTDD
AERQAAEKALAYMDLRPGTAMRDIAVDAVFVGSCTNGRIEDLRVVAEVLRGRKVADGVRMLIVPGSMRVRAQAEAEGLGE
IFTDAGAQWRQAGCSMCLGMNPDQLASGERCAATSNRNFEGRQGAGGRTHLVSPAVAAATAVRGTLSSPADLN

Sequences:

>Translated_473_residues
MALQTGEPRTLAEKIWDDHIVVSGGGCAPDLIYIDLHLVHEVTSPQAFDGLRLAGRRVRRPELTLATEDHNVPTVDIDQP
IADPVSRTQVETLRRNCAEFGIRLHSMGDIEQGIVHVVGPQLGLTQPGMTIVCGDSHTSTHGAFGALAMGIGTSEVEHVL
ATQTLPLRPFKTMAVNVDGRLPDGVSAKDIILALIAKIGTGGGQGHVIEYRGSAIESLSMEGRMTICNMSIEAGARAGMV
APDETTYAFLRGRPHAPTGAQWDTALVYWQRLRTDVGAVFDTEVYLDAASLSPFVTWGTNPGQGVPLAAAVPDPQLMTDD
AERQAAEKALAYMDLRPGTAMRDIAVDAVFVGSCTNGRIEDLRVVAEVLRGRKVADGVRMLIVPGSMRVRAQAEAEGLGE
IFTDAGAQWRQAGCSMCLGMNPDQLASGERCAATSNRNFEGRQGAGGRTHLVSPAVAAATAVRGTLSSPADLN
>Mature_472_residues
ALQTGEPRTLAEKIWDDHIVVSGGGCAPDLIYIDLHLVHEVTSPQAFDGLRLAGRRVRRPELTLATEDHNVPTVDIDQPI
ADPVSRTQVETLRRNCAEFGIRLHSMGDIEQGIVHVVGPQLGLTQPGMTIVCGDSHTSTHGAFGALAMGIGTSEVEHVLA
TQTLPLRPFKTMAVNVDGRLPDGVSAKDIILALIAKIGTGGGQGHVIEYRGSAIESLSMEGRMTICNMSIEAGARAGMVA
PDETTYAFLRGRPHAPTGAQWDTALVYWQRLRTDVGAVFDTEVYLDAASLSPFVTWGTNPGQGVPLAAAVPDPQLMTDDA
ERQAAEKALAYMDLRPGTAMRDIAVDAVFVGSCTNGRIEDLRVVAEVLRGRKVADGVRMLIVPGSMRVRAQAEAEGLGEI
FTDAGAQWRQAGCSMCLGMNPDQLASGERCAATSNRNFEGRQGAGGRTHLVSPAVAAATAVRGTLSSPADLN

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=381, Percent_Identity=28.0839895013123, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI41352693, Length=370, Percent_Identity=24.0540540540541, Blast_Score=77, Evalue=2e-14,
Organism=Escherichia coli, GI1786259, Length=459, Percent_Identity=61.2200435729847, Blast_Score=567, Evalue=1e-163,
Organism=Escherichia coli, GI1787531, Length=360, Percent_Identity=25, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87081781, Length=367, Percent_Identity=24.7956403269755, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI2367097, Length=138, Percent_Identity=34.0579710144928, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI32564738, Length=378, Percent_Identity=28.8359788359788, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI25149337, Length=378, Percent_Identity=28.8359788359788, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI25149342, Length=317, Percent_Identity=29.3375394321767, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17568399, Length=390, Percent_Identity=26.1538461538462, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321429, Length=454, Percent_Identity=57.488986784141, Blast_Score=545, Evalue=1e-156,
Organism=Saccharomyces cerevisiae, GI6320440, Length=451, Percent_Identity=27.0509977827051, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6322261, Length=402, Percent_Identity=28.6069651741293, Blast_Score=136, Evalue=8e-33,
Organism=Saccharomyces cerevisiae, GI6323335, Length=366, Percent_Identity=28.1420765027322, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI161076999, Length=395, Percent_Identity=26.5822784810127, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI281365315, Length=395, Percent_Identity=26.5822784810127, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI17864292, Length=395, Percent_Identity=26.5822784810127, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI28571643, Length=401, Percent_Identity=26.9326683291771, Blast_Score=112, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24645686, Length=370, Percent_Identity=24.3243243243243, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI17137564, Length=370, Percent_Identity=24.0540540540541, Blast_Score=65, Evalue=9e-11,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 50186; Mature: 50054

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALQTGEPRTLAEKIWDDHIVVSGGGCAPDLIYIDLHLVHEVTSPQAFDGLRLAGRRVRR
CCCCCCCCHHHHHHHCCCEEEEECCCCCCEEEEEEEEEEHHCCCCCHHCCHHHHHHHCCC
PELTLATEDHNVPTVDIDQPIADPVSRTQVETLRRNCAEFGIRLHSMGDIEQGIVHVVGP
CCEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCEEEECCCCHHHCCEEEECC
QLGLTQPGMTIVCGDSHTSTHGAFGALAMGIGTSEVEHVLATQTLPLRPFKTMAVNVDGR
CCCCCCCCCEEEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEEECCC
LPDGVSAKDIILALIAKIGTGGGQGHVIEYRGSAIESLSMEGRMTICNMSIEAGARAGMV
CCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHCCCCCEEEEEEEHHCCCCCCCC
APDETTYAFLRGRPHAPTGAQWDTALVYWQRLRTDVGAVFDTEVYLDAASLSPFVTWGTN
CCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEECEEEEECCCCCCEEEECCC
PGQGVPLAAAVPDPQLMTDDAERQAAEKALAYMDLRPGTAMRDIAVDAVFVGSCTNGRIE
CCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHEECCCCCCHHHHEEEEEEEECCCCCCHH
DLRVVAEVLRGRKVADGVRMLIVPGSMRVRAQAEAEGLGEIFTDAGAQWRQAGCSMCLGM
HHHHHHHHHCCCCCCCCEEEEEECCCCEEEECCCHHHHHHHHHHCCHHHHHHCCCEEECC
NPDQLASGERCAATSNRNFEGRQGAGGRTHLVSPAVAAATAVRGTLSSPADLN
CHHHHCCCCCEECCCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ALQTGEPRTLAEKIWDDHIVVSGGGCAPDLIYIDLHLVHEVTSPQAFDGLRLAGRRVRR
CCCCCCCHHHHHHHCCCEEEEECCCCCCEEEEEEEEEEHHCCCCCHHCCHHHHHHHCCC
PELTLATEDHNVPTVDIDQPIADPVSRTQVETLRRNCAEFGIRLHSMGDIEQGIVHVVGP
CCEEEEECCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHCEEEECCCCHHHCCEEEECC
QLGLTQPGMTIVCGDSHTSTHGAFGALAMGIGTSEVEHVLATQTLPLRPFKTMAVNVDGR
CCCCCCCCCEEEECCCCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEEEECCC
LPDGVSAKDIILALIAKIGTGGGQGHVIEYRGSAIESLSMEGRMTICNMSIEAGARAGMV
CCCCCCHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHCCCCCEEEEEEEHHCCCCCCCC
APDETTYAFLRGRPHAPTGAQWDTALVYWQRLRTDVGAVFDTEVYLDAASLSPFVTWGTN
CCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCEEECEEEEECCCCCCEEEECCC
PGQGVPLAAAVPDPQLMTDDAERQAAEKALAYMDLRPGTAMRDIAVDAVFVGSCTNGRIE
CCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHEECCCCCCHHHHEEEEEEEECCCCCCHH
DLRVVAEVLRGRKVADGVRMLIVPGSMRVRAQAEAEGLGEIFTDAGAQWRQAGCSMCLGM
HHHHHHHHHCCCCCCCCEEEEEECCCCEEEECCCHHHHHHHHHHCCHHHHHHCCCEEECC
NPDQLASGERCAATSNRNFEGRQGAGGRTHLVSPAVAAATAVRGTLSSPADLN
CHHHHCCCCCEECCCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA