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The map label for this gene is leuD

Identifier: 148824177

GI number: 148824177

Start: 3355742

End: 3356338

Strand: Reverse

Name: leuD

Synonym: TBFG_13002

Alternate gene names: 148824177

Gene position: 3356338-3355742 (Counterclockwise)

Preceding gene: 148824178

Following gene: 148824176

Centisome position: 75.86

GC content: 59.97

Gene sequence:

>597_bases
ATGGAAGCCTTTCACACCCACTCTGGTATTGGCGTGCCGTTGCGGCGGTCCAATGTCGACACCGATCAGATCATTCCCGC
GGTCTTTCTGAAGCGGGTCACCCGAACCGGTTTCGAGGACGGCTTGTTCGCGGGTTGGCGGTCGGATCCTGCATTCGTGC
TAAACCTCAGTCCCTTTGACCGGGGTTCGGTCCTGGTTGCCGGGCCCGATTTCGGCACCGGATCCTCGCGAGAGCATGCG
GTCTGGGCGCTCATGGACTACGGATTCCGGGTGGTTATCTCATCCCGATTTGGTGACATTTTCCGCGGCAACGCGGGCAA
GGCCGGGCTGCTGGCGGCCGAGGTTGCCCAAGACGATGTGGAACTCCTGTGGAAGCTCATTGAGCAGAGTCCGGGCCTGG
AAATCACTGCCAATCTTCAAGATCGAATTATCACCGCAGCAACAGTGGTGCTGCCGTTCAAGATTGACGACCACTCGGCG
TGGCGGCTGCTTGAAGGTCTCGACGATATAGCCCTTACGCTGCGGAAACTCGACGAGATCGAGGCCTTTGAGGGTGCGTG
CGCGTACTGGAAACCGCGCACTCTACCCGCCCCCTGA

Upstream 100 bases:

>100_bases
ACATTTGGTGTCCCCAGCAGTGGCGGCCGCCACCGCGGTTCGCGGCACACTGTCCAGCCCGGCCGATTTGAATTGAACTG
AGAACAGCTAGGGGATCAGC

Downstream 100 bases:

>100_bases
GGCGTAGAACCGGGCGAAATTTCAGCGTGACCCGCCGGCAGAACCTGAGCCATTTTGGGGCCGCGCCACCGGTCAAGGGC
GGCAACCGGATTGCGAAAAA

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MEAFHTHSGIGVPLRRSNVDTDQIIPAVFLKRVTRTGFEDGLFAGWRSDPAFVLNLSPFDRGSVLVAGPDFGTGSSREHA
VWALMDYGFRVVISSRFGDIFRGNAGKAGLLAAEVAQDDVELLWKLIEQSPGLEITANLQDRIITAATVVLPFKIDDHSA
WRLLEGLDDIALTLRKLDEIEAFEGACAYWKPRTLPAP

Sequences:

>Translated_198_residues
MEAFHTHSGIGVPLRRSNVDTDQIIPAVFLKRVTRTGFEDGLFAGWRSDPAFVLNLSPFDRGSVLVAGPDFGTGSSREHA
VWALMDYGFRVVISSRFGDIFRGNAGKAGLLAAEVAQDDVELLWKLIEQSPGLEITANLQDRIITAATVVLPFKIDDHSA
WRLLEGLDDIALTLRKLDEIEAFEGACAYWKPRTLPAP
>Mature_198_residues
MEAFHTHSGIGVPLRRSNVDTDQIIPAVFLKRVTRTGFEDGLFAGWRSDPAFVLNLSPFDRGSVLVAGPDFGTGSSREHA
VWALMDYGFRVVISSRFGDIFRGNAGKAGLLAAEVAQDDVELLWKLIEQSPGLEITANLQDRIITAATVVLPFKIDDHSA
WRLLEGLDDIALTLRKLDEIEAFEGACAYWKPRTLPAP

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=192, Percent_Identity=43.2291666666667, Blast_Score=155, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6321429, Length=198, Percent_Identity=39.8989898989899, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_MYCBO (P65278)

Other databases:

- EMBL:   BX248344
- RefSeq:   NP_856656.1
- ProteinModelPortal:   P65278
- SMR:   P65278
- EnsemblBacteria:   EBMYCT00000017111
- GeneID:   1093409
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb3011c
- GeneTree:   EBGT00050000015493
- HOGENOM:   HBG304838
- OMA:   DEISITM
- ProtClustDB:   PRK01641
- BioCyc:   MBOV233413:MB3011C-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670:SF2
- PANTHER:   PTHR11670
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 21780; Mature: 21780

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAFHTHSGIGVPLRRSNVDTDQIIPAVFLKRVTRTGFEDGLFAGWRSDPAFVLNLSPFD
CCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCCEEEEECCCCC
RGSVLVAGPDFGTGSSREHAVWALMDYGFRVVISSRFGDIFRGNAGKAGLLAAEVAQDDV
CCCEEEECCCCCCCCCCCCEEEEEECCCEEEEEECCCCHHHCCCCCCCCHHHHHHHHHHH
ELLWKLIEQSPGLEITANLQDRIITAATVVLPFKIDDHSAWRLLEGLDDIALTLRKLDEI
HHHHHHHHCCCCCEEEECHHHHEEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
EAFEGACAYWKPRTLPAP
HHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEAFHTHSGIGVPLRRSNVDTDQIIPAVFLKRVTRTGFEDGLFAGWRSDPAFVLNLSPFD
CCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEECCCCCCEEEEECCCCC
RGSVLVAGPDFGTGSSREHAVWALMDYGFRVVISSRFGDIFRGNAGKAGLLAAEVAQDDV
CCCEEEECCCCCCCCCCCCEEEEEECCCEEEEEECCCCHHHCCCCCCCCHHHHHHHHHHH
ELLWKLIEQSPGLEITANLQDRIITAATVVLPFKIDDHSAWRLLEGLDDIALTLRKLDEI
HHHHHHHHCCCCCEEEECHHHHEEEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHH
EAFEGACAYWKPRTLPAP
HHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972