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The map label for this gene is fadD26 [H]

Identifier: 148824119

GI number: 148824119

Start: 3255041

End: 3256921

Strand: Direct

Name: fadD26 [H]

Synonym: TBFG_12944

Alternate gene names: 148824119

Gene position: 3255041-3256921 (Clockwise)

Preceding gene: 148824118

Following gene: 148824120

Centisome position: 73.57

GC content: 60.71

Gene sequence:

>1881_bases
GTGGCTTGGTCAGTTCAGACCGGCACGTTTCAGAGCGGTGGGGATGTCCCGACGTGCGATCCAACAGGGGTTCGCAGGGT
CCGCAAAAAACATAGTGAACGCCAGAAAGCCGAATGGGAGTACAAGGCGATGCCGGTGACCGACCGTTCAGTGCCCTCTT
TGCTGCAAGAGAGGGCCGACCAGCAGCCTGACAGCACTGCATATACGTACATCGACTACGGATCCGACCCCAAGGGATTT
GCTGACAGCTTGACTTGGTCGCAGGTCTACAGTCGTGCATGCATCATTGCTGAAGAACTCAAGTTATGCGGGTTACCCGG
AGATCGAGTGGCGGTTTTAGCGCCACAAGGACTGGAATATGTCCTTGCATTCCTGGGCGCACTTCAGGCTGGATTTATCG
CGGTTCCGCTGTCAACTCCACAGTATGGCATTCACGATGACCGCGTTTCTGCGGTGTTGCAGGATTCCAAGCCGGTAGCC
ATTCTCACGACTTCGTCCGTGGTAGGCGATGTAACGAAATACGCAGCCAGCCACGACGGGCAGCCTGCCCCGGTCGTAGT
TGAGGTTGATCTGCTTGATTTGGACTCGCCGCGACAGATGCCGGCTTTCTCTCGTCAGCACACCGGGGCGGCTTATCTCC
AATACACGTCCGGATCGACGCGTACGCCGGCCGGAGTCATTGTGTCGCACACGAATGTCATTGCCAATGTGACACAAAGT
ATGTACGGCTATTTCGGCGATCCCGCAAAGATTCCGACCGGGACTGTGGTGTCGTGGCTGCCTTTGTATCACGATATGGG
CCTGATTCTCGGAATTTGCGCACCGCTGGTGGCCCGACGCCGCGCGATGTTGATGAGCCCAATGTCATTTTTGCGCCGTC
CGGCCCGCTGGATGCAACTGCTTGCCACCAGCGGCCGGTGCTTTTCTGCGGCACCGAATTTCGCCTTCGAGCTGGCCGTG
CGCAGAACATCTGACCAGGACATGGCGGGGCTCGACCTGCGCGACGTGGTCGGCATCGTCAGTGGCAGTGAGCGAATCCA
TGTGGCAACCGTGCGGCGGTTCATCGAGCGGTTCGCGCCGTACAATCTCAGCCCCACCGCGATACGGCCGTCGTACGGGC
TCGCGGAAGCGACCTTATATGTGGCAGCTCCCGAAGCCGGCGCCGCGCCCAAGACGGTCCGTTTTGACTACGAGCAGCTG
ACCGCCGGGCAGGCTCGGCCCTGCGGAACCGATGGGTCGGTCGGCACCGAACTGATCAGCTACGGCTCCCCCGACCCATC
GTCTGTGCGAATCGTCAACCCGGAGACCATGGTTGAGAATCCGCCTGGAGTGGTCGGTGAGATCTGGGTGCATGGCGACC
ACGTGACTATGGGGTATTGGCAGAAGCCGAAGCAGACCGCGCAGGTCTTCGACGCCAAGCTGGTCGATCCCGCGCCGGCA
GCCCCGGAGGGGCCGTGGCTGCGCACCGGCGACCTGGGCGTCATTTCCGATGGTGAGCTGTTCATCATGGGCCGCATCAA
AGACCTGCTCATCGTGGACGGGCGCAACCACTACCCCGACGACATCGAGGCAACGATCCAGGAGATCACCGGTGGACGGG
CCGCGGCGATCGCAGTGCCCGACGACATCACCGAACAACTGGTGGCGATCATCGAATTCAAGCGACGCGGTAGTACCGCC
GAAGAGGTCATGCTCAAGCTCCGCTCGGTGAAGCGTGAGGTCACCTCCGCGATATCGAAGTCACACAGCCTGCGGGTGGC
CGATCTCGTTCTGGTGTCACCTGGTTCGATTCCCATCACCACCAGCGGCAAGATCCGGCGGTCAGCCTGCGTCGAACGCT
ATCGCAGCGACGGCTTCAAGCGGCTGGACGTAGCCGTATGA

Upstream 100 bases:

>100_bases
ATTTGCGGGCTAGGCATAGCGGAGCAGGGGTTTTAGTCTAGGGGGAGATCGGCTGGCGCTGCGCAGACATGCTGCGGAAG
CAGAACTGCGTAATCGTCAG

Downstream 100 bases:

>100_bases
CGGGAAGCATCAGTGGTGAAGCCGACCTTCGCCACTGGCTAATCGACTACCTAGTAACCAATATCGGCTGCACACCTGAC
GAGGTGGACCCCGATCTGTC

Product: acyl-CoA synthetase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 626; Mature: 625

Protein sequence:

>626_residues
MAWSVQTGTFQSGGDVPTCDPTGVRRVRKKHSERQKAEWEYKAMPVTDRSVPSLLQERADQQPDSTAYTYIDYGSDPKGF
ADSLTWSQVYSRACIIAEELKLCGLPGDRVAVLAPQGLEYVLAFLGALQAGFIAVPLSTPQYGIHDDRVSAVLQDSKPVA
ILTTSSVVGDVTKYAASHDGQPAPVVVEVDLLDLDSPRQMPAFSRQHTGAAYLQYTSGSTRTPAGVIVSHTNVIANVTQS
MYGYFGDPAKIPTGTVVSWLPLYHDMGLILGICAPLVARRRAMLMSPMSFLRRPARWMQLLATSGRCFSAAPNFAFELAV
RRTSDQDMAGLDLRDVVGIVSGSERIHVATVRRFIERFAPYNLSPTAIRPSYGLAEATLYVAAPEAGAAPKTVRFDYEQL
TAGQARPCGTDGSVGTELISYGSPDPSSVRIVNPETMVENPPGVVGEIWVHGDHVTMGYWQKPKQTAQVFDAKLVDPAPA
APEGPWLRTGDLGVISDGELFIMGRIKDLLIVDGRNHYPDDIEATIQEITGGRAAAIAVPDDITEQLVAIIEFKRRGSTA
EEVMLKLRSVKREVTSAISKSHSLRVADLVLVSPGSIPITTSGKIRRSACVERYRSDGFKRLDVAV

Sequences:

>Translated_626_residues
MAWSVQTGTFQSGGDVPTCDPTGVRRVRKKHSERQKAEWEYKAMPVTDRSVPSLLQERADQQPDSTAYTYIDYGSDPKGF
ADSLTWSQVYSRACIIAEELKLCGLPGDRVAVLAPQGLEYVLAFLGALQAGFIAVPLSTPQYGIHDDRVSAVLQDSKPVA
ILTTSSVVGDVTKYAASHDGQPAPVVVEVDLLDLDSPRQMPAFSRQHTGAAYLQYTSGSTRTPAGVIVSHTNVIANVTQS
MYGYFGDPAKIPTGTVVSWLPLYHDMGLILGICAPLVARRRAMLMSPMSFLRRPARWMQLLATSGRCFSAAPNFAFELAV
RRTSDQDMAGLDLRDVVGIVSGSERIHVATVRRFIERFAPYNLSPTAIRPSYGLAEATLYVAAPEAGAAPKTVRFDYEQL
TAGQARPCGTDGSVGTELISYGSPDPSSVRIVNPETMVENPPGVVGEIWVHGDHVTMGYWQKPKQTAQVFDAKLVDPAPA
APEGPWLRTGDLGVISDGELFIMGRIKDLLIVDGRNHYPDDIEATIQEITGGRAAAIAVPDDITEQLVAIIEFKRRGSTA
EEVMLKLRSVKREVTSAISKSHSLRVADLVLVSPGSIPITTSGKIRRSACVERYRSDGFKRLDVAV
>Mature_625_residues
AWSVQTGTFQSGGDVPTCDPTGVRRVRKKHSERQKAEWEYKAMPVTDRSVPSLLQERADQQPDSTAYTYIDYGSDPKGFA
DSLTWSQVYSRACIIAEELKLCGLPGDRVAVLAPQGLEYVLAFLGALQAGFIAVPLSTPQYGIHDDRVSAVLQDSKPVAI
LTTSSVVGDVTKYAASHDGQPAPVVVEVDLLDLDSPRQMPAFSRQHTGAAYLQYTSGSTRTPAGVIVSHTNVIANVTQSM
YGYFGDPAKIPTGTVVSWLPLYHDMGLILGICAPLVARRRAMLMSPMSFLRRPARWMQLLATSGRCFSAAPNFAFELAVR
RTSDQDMAGLDLRDVVGIVSGSERIHVATVRRFIERFAPYNLSPTAIRPSYGLAEATLYVAAPEAGAAPKTVRFDYEQLT
AGQARPCGTDGSVGTELISYGSPDPSSVRIVNPETMVENPPGVVGEIWVHGDHVTMGYWQKPKQTAQVFDAKLVDPAPAA
PEGPWLRTGDLGVISDGELFIMGRIKDLLIVDGRNHYPDDIEATIQEITGGRAAAIAVPDDITEQLVAIIEFKRRGSTAE
EVMLKLRSVKREVTSAISKSHSLRVADLVLVSPGSIPITTSGKIRRSACVERYRSDGFKRLDVAV

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=613, Percent_Identity=26.2642740619902, Blast_Score=120, Evalue=5e-27,
Organism=Homo sapiens, GI55749758, Length=605, Percent_Identity=26.1157024793388, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI225735629, Length=604, Percent_Identity=24.1721854304636, Blast_Score=97, Evalue=7e-20,
Organism=Homo sapiens, GI45827692, Length=604, Percent_Identity=24.1721854304636, Blast_Score=96, Evalue=7e-20,
Organism=Homo sapiens, GI225735625, Length=609, Percent_Identity=25.7799671592775, Blast_Score=92, Evalue=2e-18,
Organism=Homo sapiens, GI187761345, Length=571, Percent_Identity=24.6935201401051, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI187761343, Length=571, Percent_Identity=24.6935201401051, Blast_Score=89, Evalue=1e-17,
Organism=Escherichia coli, GI1788107, Length=586, Percent_Identity=25.7679180887372, Blast_Score=115, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17531443, Length=598, Percent_Identity=22.5752508361204, Blast_Score=99, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI193202679, Length=620, Percent_Identity=24.3548387096774, Blast_Score=97, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17559526, Length=532, Percent_Identity=25.9398496240602, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24654656, Length=475, Percent_Identity=23.5789473684211, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI281365686, Length=171, Percent_Identity=30.9941520467836, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 67936; Mature: 67805

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAWSVQTGTFQSGGDVPTCDPTGVRRVRKKHSERQKAEWEYKAMPVTDRSVPSLLQERAD
CCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHC
QQPDSTAYTYIDYGSDPKGFADSLTWSQVYSRACIIAEELKLCGLPGDRVAVLAPQGLEY
CCCCCCEEEEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHH
VLAFLGALQAGFIAVPLSTPQYGIHDDRVSAVLQDSKPVAILTTSSVVGDVTKYAASHDG
HHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHCCCC
QPAPVVVEVDLLDLDSPRQMPAFSRQHTGAAYLQYTSGSTRTPAGVIVSHTNVIANVTQS
CCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEECCHHHHHHHHH
MYGYFGDPAKIPTGTVVSWLPLYHDMGLILGICAPLVARRRAMLMSPMSFLRRPARWMQL
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LATSGRCFSAAPNFAFELAVRRTSDQDMAGLDLRDVVGIVSGSERIHVATVRRFIERFAP
HHCCCCEEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHCC
YNLSPTAIRPSYGLAEATLYVAAPEAGAAPKTVRFDYEQLTAGQARPCGTDGSVGTELIS
CCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEECHHHHCCCCCCCCCCCCCHHHHHHH
YGSPDPSSVRIVNPETMVENPPGVVGEIWVHGDHVTMGYWQKPKQTAQVFDAKLVDPAPA
CCCCCCCCEEEECCHHHCCCCCCCEEEEEEECCEEEECCCCCCHHHHHHHHHHCCCCCCC
APEGPWLRTGDLGVISDGELFIMGRIKDLLIVDGRNHYPDDIEATIQEITGGRAAAIAVP
CCCCCEEECCCEEEECCCCEEEEECCCEEEEECCCCCCCCHHHHHHHHHCCCCEEEEECC
DDITEQLVAIIEFKRRGSTAEEVMLKLRSVKREVTSAISKSHSLRVADLVLVSPGSIPIT
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCEEEE
TSGKIRRSACVERYRSDGFKRLDVAV
CCCCHHHHHHHHHHHCCCCCEEEECC
>Mature Secondary Structure 
AWSVQTGTFQSGGDVPTCDPTGVRRVRKKHSERQKAEWEYKAMPVTDRSVPSLLQERAD
CEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHC
QQPDSTAYTYIDYGSDPKGFADSLTWSQVYSRACIIAEELKLCGLPGDRVAVLAPQGLEY
CCCCCCEEEEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCHHH
VLAFLGALQAGFIAVPLSTPQYGIHDDRVSAVLQDSKPVAILTTSSVVGDVTKYAASHDG
HHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHCCCC
QPAPVVVEVDLLDLDSPRQMPAFSRQHTGAAYLQYTSGSTRTPAGVIVSHTNVIANVTQS
CCCCEEEEEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEECCHHHHHHHHH
MYGYFGDPAKIPTGTVVSWLPLYHDMGLILGICAPLVARRRAMLMSPMSFLRRPARWMQL
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LATSGRCFSAAPNFAFELAVRRTSDQDMAGLDLRDVVGIVSGSERIHVATVRRFIERFAP
HHCCCCEEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHCC
YNLSPTAIRPSYGLAEATLYVAAPEAGAAPKTVRFDYEQLTAGQARPCGTDGSVGTELIS
CCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEECHHHHCCCCCCCCCCCCCHHHHHHH
YGSPDPSSVRIVNPETMVENPPGVVGEIWVHGDHVTMGYWQKPKQTAQVFDAKLVDPAPA
CCCCCCCCEEEECCHHHCCCCCCCEEEEEEECCEEEECCCCCCHHHHHHHHHHCCCCCCC
APEGPWLRTGDLGVISDGELFIMGRIKDLLIVDGRNHYPDDIEATIQEITGGRAAAIAVP
CCCCCEEECCCEEEECCCCEEEEECCCEEEEECCCCCCCCHHHHHHHHHCCCCEEEEECC
DDITEQLVAIIEFKRRGSTAEEVMLKLRSVKREVTSAISKSHSLRVADLVLVSPGSIPIT
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCCEEEE
TSGKIRRSACVERYRSDGFKRLDVAV
CCCCHHHHHHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0024 {myristate}} 0.0021 {myristate}} 0.0012 {myristate}} 0.0002 {myristate}} 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0112 {hexanoate}} 0.0083 {decanoate}} 0.0063 {decanoate}} 0.0043 {dec

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]