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The map label for this gene is echA8 [H]

Identifier: 148824021

GI number: 148824021

Start: 3148909

End: 3149658

Strand: Direct

Name: echA8 [H]

Synonym: TBFG_12846

Alternate gene names: 148824021

Gene position: 3148909-3149658 (Clockwise)

Preceding gene: 148824002

Following gene: 148824033

Centisome position: 71.17

GC content: 70.53

Gene sequence:

>750_bases
ATGACCGACGACATCCTGCTGATCGACACCGACGAACGGGTGCGAACCCTCACCCTCAACCGGCCGCAGTCCCGCAACGC
GCTCTCGGCGGCGCTACGGGATCGGTTTTTCGCGGCGTTGGCCGACGCCGAGGCCGACGACGACATCGACGTCGTCATCC
TCACCGGCGCCGATCCGGTGTTCTGCGCCGGACTGGACCTCAAGGAGCTGGCCGGGCAGACCGCGCTGCCGGACATCTCA
CCGCGGTGGCCGGCCATGACCAAGCCGGTGATCGGCGCGATCAACGGCGCCGCGGTCACCGGCGGGCTCGAACTGGCGCT
GTACTGCGACATCCTGATCGCCTCCGAGCACGCCCGCTTCGCCGACACCCACGCCCGGGTGGGCCTGCTGCCCACCTGGG
GACTCAGCGTGCGCTTGCCGCAAAAGGTCGGCATCGGCCTGGCCCGGCGGATGAGCCTGACCGGCGACTACCTGTCCGCG
ACCGACGCGTTGCGGGCCGGCCTGGTCACCGAGGTGGTGGCCCACGACCAGCTGCTGCCCACCGCCCGCCGGGTGGCGGC
GTCGATCGTCGGCAACAACCAGAACGCGGTGCGGGCATTGCTGGCGTCCTACCACCGCATCGACGAGTCTCAGACCGCCG
CCGGGCTGTGGCTGGAAGCCTGCGCGGCCAAGCAATTTCGCACTAGCGGCGATACCATCGCCGCCAACCGCGAAGCCGTG
CTGCAGCGCGGCCGCGCGCAGGTGCGTTAG

Upstream 100 bases:

>100_bases
GGCCACTTCATCAAGCAAGGAGAGGATCTTCGCCTTCACCTCCGTAGCGGTCATTTTTCTGGTCATTAGGACAGTCTAAC
GGTCCTGTTACGGTGATCGA

Downstream 100 bases:

>100_bases
CGGCGATCGCAAGCGCGGCGAAGCCGGGTGCTGGGGGTACCTCCCGCGTGCGGGGGACGGGTCGCCGCCATCAGCCCTTC
AGCGAAGCCGGGTCTCGGTG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MTDDILLIDTDERVRTLTLNRPQSRNALSAALRDRFFAALADAEADDDIDVVILTGADPVFCAGLDLKELAGQTALPDIS
PRWPAMTKPVIGAINGAAVTGGLELALYCDILIASEHARFADTHARVGLLPTWGLSVRLPQKVGIGLARRMSLTGDYLSA
TDALRAGLVTEVVAHDQLLPTARRVAASIVGNNQNAVRALLASYHRIDESQTAAGLWLEACAAKQFRTSGDTIAANREAV
LQRGRAQVR

Sequences:

>Translated_249_residues
MTDDILLIDTDERVRTLTLNRPQSRNALSAALRDRFFAALADAEADDDIDVVILTGADPVFCAGLDLKELAGQTALPDIS
PRWPAMTKPVIGAINGAAVTGGLELALYCDILIASEHARFADTHARVGLLPTWGLSVRLPQKVGIGLARRMSLTGDYLSA
TDALRAGLVTEVVAHDQLLPTARRVAASIVGNNQNAVRALLASYHRIDESQTAAGLWLEACAAKQFRTSGDTIAANREAV
LQRGRAQVR
>Mature_248_residues
TDDILLIDTDERVRTLTLNRPQSRNALSAALRDRFFAALADAEADDDIDVVILTGADPVFCAGLDLKELAGQTALPDISP
RWPAMTKPVIGAINGAAVTGGLELALYCDILIASEHARFADTHARVGLLPTWGLSVRLPQKVGIGLARRMSLTGDYLSAT
DALRAGLVTEVVAHDQLLPTARRVAASIVGNNQNAVRALLASYHRIDESQTAAGLWLEACAAKQFRTSGDTIAANREAVL
QRGRAQVR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=194, Percent_Identity=39.1752577319588, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI31542718, Length=206, Percent_Identity=29.6116504854369, Blast_Score=88, Evalue=6e-18,
Organism=Homo sapiens, GI116235487, Length=183, Percent_Identity=31.1475409836066, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI20127408, Length=174, Percent_Identity=32.183908045977, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI70995211, Length=233, Percent_Identity=27.0386266094421, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI37594469, Length=212, Percent_Identity=27.8301886792453, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI37594471, Length=212, Percent_Identity=27.8301886792453, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI25453481, Length=182, Percent_Identity=27.4725274725275, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI51538573, Length=182, Percent_Identity=27.4725274725275, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4757968, Length=182, Percent_Identity=26.9230769230769, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI49355787, Length=182, Percent_Identity=26.9230769230769, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI4757966, Length=182, Percent_Identity=27.4725274725275, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI51538581, Length=182, Percent_Identity=27.4725274725275, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI221136756, Length=182, Percent_Identity=28.021978021978, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI221136753, Length=182, Percent_Identity=28.021978021978, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI221307494, Length=182, Percent_Identity=28.021978021978, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI45643119, Length=220, Percent_Identity=25.4545454545455, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI260275230, Length=220, Percent_Identity=25.4545454545455, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI4502327, Length=185, Percent_Identity=30.2702702702703, Blast_Score=68, Evalue=8e-12,
Organism=Homo sapiens, GI260274832, Length=220, Percent_Identity=25.4545454545455, Blast_Score=68, Evalue=9e-12,
Organism=Escherichia coli, GI1787659, Length=221, Percent_Identity=37.5565610859729, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI221142681, Length=205, Percent_Identity=34.1463414634146, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1788597, Length=214, Percent_Identity=33.1775700934579, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1787660, Length=196, Percent_Identity=34.6938775510204, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788682, Length=204, Percent_Identity=30.3921568627451, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790281, Length=195, Percent_Identity=27.1794871794872, Blast_Score=67, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25145438, Length=215, Percent_Identity=35.8139534883721, Blast_Score=117, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17554946, Length=192, Percent_Identity=32.8125, Blast_Score=111, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17540714, Length=192, Percent_Identity=36.4583333333333, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17560910, Length=181, Percent_Identity=32.5966850828729, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17540306, Length=188, Percent_Identity=31.9148936170213, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17549921, Length=219, Percent_Identity=29.2237442922374, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=193, Percent_Identity=26.9430051813472, Blast_Score=76, Evalue=4e-15,
Organism=Drosophila melanogaster, GI20129971, Length=196, Percent_Identity=36.734693877551, Blast_Score=121, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24653477, Length=196, Percent_Identity=36.734693877551, Blast_Score=121, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24650670, Length=194, Percent_Identity=33.5051546391753, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24653139, Length=236, Percent_Identity=28.8135593220339, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI21357171, Length=239, Percent_Identity=28.4518828451883, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI19922422, Length=191, Percent_Identity=27.2251308900524, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI24654903, Length=184, Percent_Identity=23.3695652173913, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 26631; Mature: 26499

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDDILLIDTDERVRTLTLNRPQSRNALSAALRDRFFAALADAEADDDIDVVILTGADPV
CCCCEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCE
FCAGLDLKELAGQTALPDISPRWPAMTKPVIGAINGAAVTGGLELALYCDILIASEHARF
EEECCCHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCCEEECCEEEEEEEEEEEECCCCHH
ADTHARVGLLPTWGLSVRLPQKVGIGLARRMSLTGDYLSATDALRAGLVTEVVAHDQLLP
HHCCCCEECCCCCCCEEECCHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TARRVAASIVGNNQNAVRALLASYHRIDESQTAAGLWLEACAAKQFRTSGDTIAANREAV
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEECCHHHH
LQRGRAQVR
HHCCCCCCC
>Mature Secondary Structure 
TDDILLIDTDERVRTLTLNRPQSRNALSAALRDRFFAALADAEADDDIDVVILTGADPV
CCCEEEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCE
FCAGLDLKELAGQTALPDISPRWPAMTKPVIGAINGAAVTGGLELALYCDILIASEHARF
EEECCCHHHHCCCCCCCCCCCCCCCCCCCHHHCCCCCEEECCEEEEEEEEEEEECCCCHH
ADTHARVGLLPTWGLSVRLPQKVGIGLARRMSLTGDYLSATDALRAGLVTEVVAHDQLLP
HHCCCCEECCCCCCCEEECCHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
TARRVAASIVGNNQNAVRALLASYHRIDESQTAAGLWLEACAAKQFRTSGDTIAANREAV
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCEEECCHHHH
LQRGRAQVR
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]