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The map label for this gene is pafC

Identifier: 148823309

GI number: 148823309

Start: 2369404

End: 2370354

Strand: Reverse

Name: pafC

Synonym: TBFG_12130

Alternate gene names: 148823309

Gene position: 2370354-2369404 (Counterclockwise)

Preceding gene: 148823310

Following gene: 148823308

Centisome position: 53.57

GC content: 68.56

Gene sequence:

>951_bases
ATGAGCGCCCTGTCCACCCGGCTGGTGCGGCTGCTCAACATGGTGCCGTACTTCCAGGCCAACCCCCGGATCACCCGCGC
CGAGGCCGCCGCCGAGCTGGGTGTCACCGCCAAGCAGCTGGAGGAGGACCTCAACCAGCTGTGGATGTGCGGCCTTCCGG
GCTATTCCCCGGGTGACCTCATCGACTTCGAATTCTGCGGTGACACCATCGAGGTGACGTTCTCGGCGGGCATCGACCGG
CCGTTAAAGCTCACATCGCCGGAGGCCACCGGGCTGCTGGTGGCGTTGCGGGCGCTAGCGGACATTCCCGGCGTGGTTGA
CCCGCAGGCGGCGCGCAGCGCCATCGCCAAGATCGCCGCCGCGGCCGGCGCGGTGGCCGCCGTTGCCGAACAGGCACCCA
CAGAAAGCCCGGCCGCCGCGGCCGTACGGGCCGCGGTGCGGAACAGTCGGGCGCTGACCATCGACTATTACGCCGCGTCG
CATGACACCCTCACCACTCGGATCGTGGATCCCATCCGGGTGCTGTTGATCGGCGGGCACAGCTATTTGGAGGCCTGGTC
GCGTGAGGCCGAAGGGGTTCGCCTGTTCCGTTTCGACCGGATTGTCGATGCCGCCGAGTTGGGCGAGCCCGCGGTTCCGC
CGGAATCGGCACGGCAGGCGCCACCGGACACGTCGCTGTTCGACGGTGACCTATCGCTGCCGTCGGCGACGTTGCGGGTG
GCACCGTCGGCCTCGTGGATGCTGGAGTACTACCCGATTCGGGAGCTACGACAGCTGCCGGACGGCTCCTGTGAGGTGGC
CATGACCTACGCCTCTGAGGATTGGATGACGCGCCTATTGCTGGGCTTCGGTTCGGACGTGCGCGTTCTGGCGCCGGAGT
CGCTCGCTCAGCGGGTTCGCGACGCCGCGACGGCCGCGCTGGATGCATACCAGGCAGCTGCGCCGCCGTGA

Upstream 100 bases:

>100_bases
GTGAGATCACCGGCTATGGGGCCGACGCAATCGTGCTGCAGCCCGGGTCTCTGCGCGACGATGTGCTGGCCCGACTACGC
GCGCAAGCCGGAGCACTGGC

Downstream 100 bases:

>100_bases
CGGCGCGAAATCTCCGGGTGCCGCGGCCGCTGCGGTAGCATCGGGTCAACGTCTGGAGGTAATCAAAGTGGGCAGTCTGA
GTCCGTGGCATTGGGCGATC

Product: hypothetical protein

Products: NA

Alternate protein names: Proteasome accessory factor C

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MSALSTRLVRLLNMVPYFQANPRITRAEAAAELGVTAKQLEEDLNQLWMCGLPGYSPGDLIDFEFCGDTIEVTFSAGIDR
PLKLTSPEATGLLVALRALADIPGVVDPQAARSAIAKIAAAAGAVAAVAEQAPTESPAAAAVRAAVRNSRALTIDYYAAS
HDTLTTRIVDPIRVLLIGGHSYLEAWSREAEGVRLFRFDRIVDAAELGEPAVPPESARQAPPDTSLFDGDLSLPSATLRV
APSASWMLEYYPIRELRQLPDGSCEVAMTYASEDWMTRLLLGFGSDVRVLAPESLAQRVRDAATAALDAYQAAAPP

Sequences:

>Translated_316_residues
MSALSTRLVRLLNMVPYFQANPRITRAEAAAELGVTAKQLEEDLNQLWMCGLPGYSPGDLIDFEFCGDTIEVTFSAGIDR
PLKLTSPEATGLLVALRALADIPGVVDPQAARSAIAKIAAAAGAVAAVAEQAPTESPAAAAVRAAVRNSRALTIDYYAAS
HDTLTTRIVDPIRVLLIGGHSYLEAWSREAEGVRLFRFDRIVDAAELGEPAVPPESARQAPPDTSLFDGDLSLPSATLRV
APSASWMLEYYPIRELRQLPDGSCEVAMTYASEDWMTRLLLGFGSDVRVLAPESLAQRVRDAATAALDAYQAAAPP
>Mature_315_residues
SALSTRLVRLLNMVPYFQANPRITRAEAAAELGVTAKQLEEDLNQLWMCGLPGYSPGDLIDFEFCGDTIEVTFSAGIDRP
LKLTSPEATGLLVALRALADIPGVVDPQAARSAIAKIAAAAGAVAAVAEQAPTESPAAAAVRAAVRNSRALTIDYYAASH
DTLTTRIVDPIRVLLIGGHSYLEAWSREAEGVRLFRFDRIVDAAELGEPAVPPESARQAPPDTSLFDGDLSLPSATLRVA
PSASWMLEYYPIRELRQLPDGSCEVAMTYASEDWMTRLLLGFGSDVRVLAPESLAQRVRDAATAALDAYQAAAPP

Specific function: Part of the pafABC operon, but pafC does not seem to be involved in pupylation or substrate degradation. Appears to play at least a small role in resistance to reactive nitrogen intermediates (RNI) in vitro

COG id: COG2378

COG function: function code K; Predicted transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pafC family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PAFC_MYCTU (Q10704)

Other databases:

- EMBL:   DQ990836
- EMBL:   BX842578
- EMBL:   AE000516
- PIR:   B70768
- RefSeq:   NP_216611.1
- RefSeq:   NP_336623.1
- ProteinModelPortal:   Q10704
- EnsemblBacteria:   EBMYCT00000001006
- EnsemblBacteria:   EBMYCT00000071356
- GeneID:   888403
- GeneID:   926642
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT2156
- KEGG:   mtu:Rv2095c
- TIGR:   MT2156
- TubercuList:   Rv2095c
- GeneTree:   EBGT00050000016807
- HOGENOM:   HBG428753
- OMA:   ASWMFEY
- ProtClustDB:   CLSK791625

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 33765; Mature: 33633

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALSTRLVRLLNMVPYFQANPRITRAEAAAELGVTAKQLEEDLNQLWMCGLPGYSPGDL
CCHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHCCCHHHHHHHHHHHEECCCCCCCCCCE
IDFEFCGDTIEVTFSAGIDRPLKLTSPEATGLLVALRALADIPGVVDPQAARSAIAKIAA
EEHEECCCEEEEEEECCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
AAGAVAAVAEQAPTESPAAAAVRAAVRNSRALTIDYYAASHDTLTTRIVDPIRVLLIGGH
HHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHEEEEEECCH
SYLEAWSREAEGVRLFRFDRIVDAAELGEPAVPPESARQAPPDTSLFDGDLSLPSATLRV
HHHHHHHCCCCCEEEEHHHHHHHHHHCCCCCCCCCHHCCCCCCCCEECCCCCCCCCEEEE
APSASWMLEYYPIRELRQLPDGSCEVAMTYASEDWMTRLLLGFGSDVRVLAPESLAQRVR
CCCCCHHEECCCHHHHHHCCCCCCEEEEEECCHHHHHHHHHHCCCCCEEECHHHHHHHHH
DAATAALDAYQAAAPP
HHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SALSTRLVRLLNMVPYFQANPRITRAEAAAELGVTAKQLEEDLNQLWMCGLPGYSPGDL
CHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHCCCHHHHHHHHHHHEECCCCCCCCCCE
IDFEFCGDTIEVTFSAGIDRPLKLTSPEATGLLVALRALADIPGVVDPQAARSAIAKIAA
EEHEECCCEEEEEEECCCCCCEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
AAGAVAAVAEQAPTESPAAAAVRAAVRNSRALTIDYYAASHDTLTTRIVDPIRVLLIGGH
HHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHEEEEEECCH
SYLEAWSREAEGVRLFRFDRIVDAAELGEPAVPPESARQAPPDTSLFDGDLSLPSATLRV
HHHHHHHCCCCCEEEEHHHHHHHHHHCCCCCCCCCHHCCCCCCCCEECCCCCCCCCEEEE
APSASWMLEYYPIRELRQLPDGSCEVAMTYASEDWMTRLLLGFGSDVRVLAPESLAQRVR
CCCCCHHEECCCHHHHHHCCCCCCEEEEEECCHHHHHHHHHHCCCCCEEECHHHHHHHHH
DAATAALDAYQAAAPP
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036