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The map label for this gene is ppsA [C]

Identifier: 148823263

GI number: 148823263

Start: 2306574

End: 2309138

Strand: Reverse

Name: ppsA [C]

Synonym: TBFG_12084

Alternate gene names: 148823263

Gene position: 2309138-2306574 (Counterclockwise)

Preceding gene: 148823264

Following gene: 148823261

Centisome position: 52.19

GC content: 67.37

Gene sequence:

>2565_bases
GTGAGAATCGCCGTCACCGGGGCCAGCGGAGTTCTCGGCCGCGGCCTGACCGCCAGATTGCTGAGTCAGGGTCACGAGGT
CGTCGGAATCGCACGTCATCGACCGGACAGCTGGCCGAGCTCAGCGGATTTCATTGCGGCCGATATCCGGGATGCCACCG
CCGTCGAAAGTGCTATGACAGGTGCGGATGTGGTCGCGCATTGTGCGTGGGTGCGTGGCCGAAACGACCACATCAATATC
GACGGCACCGCCAATGTCCTCAAGGCGATGGCCGAGACCGGAACCGGGCGCATCGTTTTCACGTCCAGCGGTCACCAACC
CCGCGTCGAGCAGATGCTGGCCGACTGCGGCCTGGAATGGGTCGCCGTGCGCTGCGCGCTCATTTTCGGTCGAAATGTCG
ACAACTGGGTGCAGCGGCTGTTTGCTCTGCCGGTGTTACCCGCCGGGTATGCTGATCGCGTCGTACAAGTCGTCCACTCC
GACGACGCGCAGCGGCTGTTGGTTCGCGCGCTACTGGACACGGTAATCGACAGCGGCCCTGTTAATCTCGCCGCCCCTGG
CGAGTTGACGTTTCGGCGCATTGCGGCCGCGCTCGGGCGGCCCATGGTGCCTATTGGCTCCCCAGTGCTACGACGTGTCA
CCTCGTTCGCCGAACTCGAACTCCTGCACAGCGCGCCATTGATGGATGTCACGCTCCTGCGTGACCGCTGGGGATTCCAG
CCGGCCTGGAACGCCGAGGAGTGTCTCGAAGACTTCACCCTGGCGGTGCGTGGCCGGATCGGTCTGGGAAAGCGAACATT
TTCCCTGCCTTGGCGGCTGGCTAACATCCAGGACCTTCCGGCGGTCGACTCCCCGGCAGACGACGGTGTCGCACCCAGAT
TGGCCGGCCCAGAAGGGGCCAACGGCGAGTTCGACACGCCGATCGACCCGCGCTTCCCGACCTATCTGGCCACCAACCTC
TCGGAGGCGCTGCCCGGCCCGTTCTCGCCGTCGTCGGCATCGGTGACTGTGCGGGGCCTGCGTGCCGGCGGGGTGGGCAT
CGCCGAGCGGCTGCGGCCCAGCGGGGTGATCCAGCGCGAAATCGCCATGCGGACCGTCGCGGTGTTCGCCCACCGGCTGT
ACGGGGCCATCACGTCGGCGCATTTCATGGCAGCAACCGTCCCCTTCGCCAAGCCGGCGACGATCGTCAGCAACAGCGGG
TTCTTCGGACCCAGCATGGCTTCGCTGCCCATCTTTGGCGCGCAGCGACCGCCATCGGAATCCAGCCGTGCCCGCAGGTG
GTTACGTACGCTACGCAATATCGGGGTGTTCGGCGTCAACCTGGTGGGCCTGTCCGCCGGCTCACCCCGCGACACCGATG
CCTATGTCGCCGACGTCGATCGCCTGGAGCGTCTCGCCTTCGATAACCTCGCTACCCACGACGACCGCCGGTTGTTGAGC
CTGATCTTGTTGGCACGTGACCACGTCGTGCACGGCTGGGTGCTGGCCTCGGGGTCGTTCATGCTGTGTGCGGCGTTCAA
CGTGTTGTTGCGAGGATTGTGCGGGCGGGACACCGCACCGGCGGCCGGGCCGGAGTTGGTCAGCGCGCGATCGGTGGAGG
CGGTGCAGCGGCTGGTGGCCGCGGCACGGCGGGATCCGGTCGTGATTCGGTTGCTGGCCGAGCCGGGGGAGCGTCTGGAC
AAGCTAGCTGTCGAGGCGCCGGAGTTCCATTCCGCGGTGCTGGCCGAGCTGACGCTGATCGGGCACCGCGGTCCGGCGGA
AGTCGAGATGGCCGCCACCAGCTATGCCGACAACCCCGAGTTGCTGGTGCGGATGGTAGCCAAGACGCTACGTGCGGTGC
CAGCGCCGCAACCGCCGACGCCGGTGATTCCGTTGCGGGCCAAGCCCGTTGCGTTGCTGGCGGCCCGCCAGCTCCGCGAC
CGCGAGGTCCGCCGCGACAGGATGGTGCGTGCCATCTGGGTGCTGCGTGCTCTGCTGCGGGAGTACGGGCGCCGGCTGAC
CGAGGCCGGCGTCTTCGACACCCCGGACGATGTGTTCTACCTCTTGGTCGATGAAATCGATGCGCTGCCGGCAGACGTGT
CGGGGCTGGTGGCTCGGCGCCGTGCCGAACAGCGCAGGCTTGCCGGCATCGTTCCGCCCACGGTGTTCAGCGGAAGCTGG
GAACCGTCGCCTTCGTCGGCGGCGGCGCTGGCAGCCGGGGACACTCTACGCGGAGTCGGCGTCTGCGGCGGACGGGTGCG
CGGCCGGGTGCGGATCGTGCGTCCGGAGACCATCGACGATTTGCAGCCCGGCGAGATTCTGGTCGCCGAGGTCACCGACG
TCGGATACACCGCGGCCTTCTGCTACGCCGCCGCCGTGGTGACCGAGCTCGGCGGCCCGATGTCACATGCCGCGGTGGTC
GCCCGCGAGTTCGGATTCCCTTGCGTGGTCGACGCCCAGGGAGCTACCCGGTTTCTGCCACCAGGAGCATTGGTGGAGGT
CGACGGGGCTACCGGGGAAATTCATGTGGTTGAGCTGGCGTCCGAAGATGGGCCGGCGCTTCCGGGAAGCGATCTCAGTC
GCTGA

Upstream 100 bases:

>100_bases
CAGGACCCGGCGTTGGCGCCGACCGCAGAACAAGAGATCGCGGACATGGACCTCGACGACCTGGTCAACGCGGCGTTTCG
GAACGACGACGAGTAGGACC

Downstream 100 bases:

>100_bases
ACCTAGTTCGTCGTGTCAGCTTGTGACGGGTGCACCTCAGCAGCCGAACGCAACGCCCACGCCGTCGGGTGGCGGCGCCG
GCTTCAGGCAGCCGAACGGC

Product: hypothetical protein

Products: AMP; H+; Phosphoenolpyruvate; Phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 854; Mature: 854

Protein sequence:

>854_residues
MRIAVTGASGVLGRGLTARLLSQGHEVVGIARHRPDSWPSSADFIAADIRDATAVESAMTGADVVAHCAWVRGRNDHINI
DGTANVLKAMAETGTGRIVFTSSGHQPRVEQMLADCGLEWVAVRCALIFGRNVDNWVQRLFALPVLPAGYADRVVQVVHS
DDAQRLLVRALLDTVIDSGPVNLAAPGELTFRRIAAALGRPMVPIGSPVLRRVTSFAELELLHSAPLMDVTLLRDRWGFQ
PAWNAEECLEDFTLAVRGRIGLGKRTFSLPWRLANIQDLPAVDSPADDGVAPRLAGPEGANGEFDTPIDPRFPTYLATNL
SEALPGPFSPSSASVTVRGLRAGGVGIAERLRPSGVIQREIAMRTVAVFAHRLYGAITSAHFMAATVPFAKPATIVSNSG
FFGPSMASLPIFGAQRPPSESSRARRWLRTLRNIGVFGVNLVGLSAGSPRDTDAYVADVDRLERLAFDNLATHDDRRLLS
LILLARDHVVHGWVLASGSFMLCAAFNVLLRGLCGRDTAPAAGPELVSARSVEAVQRLVAAARRDPVVIRLLAEPGERLD
KLAVEAPEFHSAVLAELTLIGHRGPAEVEMAATSYADNPELLVRMVAKTLRAVPAPQPPTPVIPLRAKPVALLAARQLRD
REVRRDRMVRAIWVLRALLREYGRRLTEAGVFDTPDDVFYLLVDEIDALPADVSGLVARRRAEQRRLAGIVPPTVFSGSW
EPSPSSAAALAAGDTLRGVGVCGGRVRGRVRIVRPETIDDLQPGEILVAEVTDVGYTAAFCYAAAVVTELGGPMSHAAVV
AREFGFPCVVDAQGATRFLPPGALVEVDGATGEIHVVELASEDGPALPGSDLSR

Sequences:

>Translated_854_residues
MRIAVTGASGVLGRGLTARLLSQGHEVVGIARHRPDSWPSSADFIAADIRDATAVESAMTGADVVAHCAWVRGRNDHINI
DGTANVLKAMAETGTGRIVFTSSGHQPRVEQMLADCGLEWVAVRCALIFGRNVDNWVQRLFALPVLPAGYADRVVQVVHS
DDAQRLLVRALLDTVIDSGPVNLAAPGELTFRRIAAALGRPMVPIGSPVLRRVTSFAELELLHSAPLMDVTLLRDRWGFQ
PAWNAEECLEDFTLAVRGRIGLGKRTFSLPWRLANIQDLPAVDSPADDGVAPRLAGPEGANGEFDTPIDPRFPTYLATNL
SEALPGPFSPSSASVTVRGLRAGGVGIAERLRPSGVIQREIAMRTVAVFAHRLYGAITSAHFMAATVPFAKPATIVSNSG
FFGPSMASLPIFGAQRPPSESSRARRWLRTLRNIGVFGVNLVGLSAGSPRDTDAYVADVDRLERLAFDNLATHDDRRLLS
LILLARDHVVHGWVLASGSFMLCAAFNVLLRGLCGRDTAPAAGPELVSARSVEAVQRLVAAARRDPVVIRLLAEPGERLD
KLAVEAPEFHSAVLAELTLIGHRGPAEVEMAATSYADNPELLVRMVAKTLRAVPAPQPPTPVIPLRAKPVALLAARQLRD
REVRRDRMVRAIWVLRALLREYGRRLTEAGVFDTPDDVFYLLVDEIDALPADVSGLVARRRAEQRRLAGIVPPTVFSGSW
EPSPSSAAALAAGDTLRGVGVCGGRVRGRVRIVRPETIDDLQPGEILVAEVTDVGYTAAFCYAAAVVTELGGPMSHAAVV
AREFGFPCVVDAQGATRFLPPGALVEVDGATGEIHVVELASEDGPALPGSDLSR
>Mature_854_residues
MRIAVTGASGVLGRGLTARLLSQGHEVVGIARHRPDSWPSSADFIAADIRDATAVESAMTGADVVAHCAWVRGRNDHINI
DGTANVLKAMAETGTGRIVFTSSGHQPRVEQMLADCGLEWVAVRCALIFGRNVDNWVQRLFALPVLPAGYADRVVQVVHS
DDAQRLLVRALLDTVIDSGPVNLAAPGELTFRRIAAALGRPMVPIGSPVLRRVTSFAELELLHSAPLMDVTLLRDRWGFQ
PAWNAEECLEDFTLAVRGRIGLGKRTFSLPWRLANIQDLPAVDSPADDGVAPRLAGPEGANGEFDTPIDPRFPTYLATNL
SEALPGPFSPSSASVTVRGLRAGGVGIAERLRPSGVIQREIAMRTVAVFAHRLYGAITSAHFMAATVPFAKPATIVSNSG
FFGPSMASLPIFGAQRPPSESSRARRWLRTLRNIGVFGVNLVGLSAGSPRDTDAYVADVDRLERLAFDNLATHDDRRLLS
LILLARDHVVHGWVLASGSFMLCAAFNVLLRGLCGRDTAPAAGPELVSARSVEAVQRLVAAARRDPVVIRLLAEPGERLD
KLAVEAPEFHSAVLAELTLIGHRGPAEVEMAATSYADNPELLVRMVAKTLRAVPAPQPPTPVIPLRAKPVALLAARQLRD
REVRRDRMVRAIWVLRALLREYGRRLTEAGVFDTPDDVFYLLVDEIDALPADVSGLVARRRAEQRRLAGIVPPTVFSGSW
EPSPSSAAALAAGDTLRGVGVCGGRVRGRVRIVRPETIDDLQPGEILVAEVTDVGYTAAFCYAAAVVTELGGPMSHAAVV
AREFGFPCVVDAQGATRFLPPGALVEVDGATGEIHVVELASEDGPALPGSDLSR

Specific function: ESSENTIAL STEP IN GLUCONEOGENESIS WHEN PYRUVATE AND LACTATE ARE USED AS A CARBON SOURCE. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family

Homologues:

Organism=Caenorhabditis elegans, GI17564524, Length=446, Percent_Identity=25.7847533632287, Blast_Score=142, Evalue=8e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y2047_MYCTU (P65684)

Other databases:

- EMBL:   BX842578
- EMBL:   AE000516
- PIR:   F70944
- RefSeq:   NP_216563.1
- RefSeq:   NP_336572.1
- ProteinModelPortal:   P65684
- EnsemblBacteria:   EBMYCT00000002549
- EnsemblBacteria:   EBMYCT00000071835
- GeneID:   888529
- GeneID:   924639
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT2107
- KEGG:   mtu:Rv2047c
- TIGR:   MT2107
- TubercuList:   Rv2047c
- GeneTree:   EBGT00050000015187
- HOGENOM:   HBG565212
- OMA:   FIADVDR
- ProtClustDB:   PRK05865
- GO:   GO:0016310
- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008279
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:3.50.30.10

Pfam domain/function: PF01370 Epimerase; PF00391 PEP-utilizers; SSF52009 PEP_mobile

EC number: 2.7.9.2 [C]

Molecular weight: Translated: 91386; Mature: 91386

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIAVTGASGVLGRGLTARLLSQGHEVVGIARHRPDSWPSSADFIAADIRDATAVESAMT
CEEEEECCCCCHHCCHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHH
GADVVAHCAWVRGRNDHINIDGTANVLKAMAETGTGRIVFTSSGHQPRVEQMLADCGLEW
HHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCHHH
VAVRCALIFGRNVDNWVQRLFALPVLPAGYADRVVQVVHSDDAQRLLVRALLDTVIDSGP
HHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
VNLAAPGELTFRRIAAALGRPMVPIGSPVLRRVTSFAELELLHSAPLMDVTLLRDRWGFQ
CCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCC
PAWNAEECLEDFTLAVRGRIGLGKRTFSLPWRLANIQDLPAVDSPADDGVAPRLAGPEGA
CCCCHHHHHHHHHHHHCCCCCCCCEEEECCEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
NGEFDTPIDPRFPTYLATNLSEALPGPFSPSSASVTVRGLRAGGVGIAERLRPSGVIQRE
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCHHHHCCCCCHHHHH
IAMRTVAVFAHRLYGAITSAHFMAATVPFAKPATIVSNSGFFGPSMASLPIFGAQRPPSE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCH
SSRARRWLRTLRNIGVFGVNLVGLSAGSPRDTDAYVADVDRLERLAFDNLATHDDRRLLS
HHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
LILLARDHVVHGWVLASGSFMLCAAFNVLLRGLCGRDTAPAAGPELVSARSVEAVQRLVA
HHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
AARRDPVVIRLLAEPGERLDKLAVEAPEFHSAVLAELTLIGHRGPAEVEMAATSYADNPE
HHCCCCEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEHHCCCCCHH
LLVRMVAKTLRAVPAPQPPTPVIPLRAKPVALLAARQLRDREVRRDRMVRAIWVLRALLR
HHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EYGRRLTEAGVFDTPDDVFYLLVDEIDALPADVSGLVARRRAEQRRLAGIVPPTVFSGSW
HHHHHHHHCCCCCCCCHHEEEEEHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
EPSPSSAAALAAGDTLRGVGVCGGRVRGRVRIVRPETIDDLQPGEILVAEVTDVGYTAAF
CCCCCCCCEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCHHHHH
CYAAAVVTELGGPMSHAAVVAREFGFPCVVDAQGATRFLPPGALVEVDGATGEIHVVELA
HHHHHHHHHHCCCHHHHHHHHHHCCCCEEEECCCCCEECCCCCEEEECCCCCEEEEEEEE
SEDGPALPGSDLSR
CCCCCCCCCCCCCC
>Mature Secondary Structure
MRIAVTGASGVLGRGLTARLLSQGHEVVGIARHRPDSWPSSADFIAADIRDATAVESAMT
CEEEEECCCCCHHCCHHHHHHHCCCCEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHH
GADVVAHCAWVRGRNDHINIDGTANVLKAMAETGTGRIVFTSSGHQPRVEQMLADCGLEW
HHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCHHH
VAVRCALIFGRNVDNWVQRLFALPVLPAGYADRVVQVVHSDDAQRLLVRALLDTVIDSGP
HHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
VNLAAPGELTFRRIAAALGRPMVPIGSPVLRRVTSFAELELLHSAPLMDVTLLRDRWGFQ
CCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCC
PAWNAEECLEDFTLAVRGRIGLGKRTFSLPWRLANIQDLPAVDSPADDGVAPRLAGPEGA
CCCCHHHHHHHHHHHHCCCCCCCCEEEECCEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
NGEFDTPIDPRFPTYLATNLSEALPGPFSPSSASVTVRGLRAGGVGIAERLRPSGVIQRE
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCHHHHCCCCCHHHHH
IAMRTVAVFAHRLYGAITSAHFMAATVPFAKPATIVSNSGFFGPSMASLPIFGAQRPPSE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCH
SSRARRWLRTLRNIGVFGVNLVGLSAGSPRDTDAYVADVDRLERLAFDNLATHDDRRLLS
HHHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
LILLARDHVVHGWVLASGSFMLCAAFNVLLRGLCGRDTAPAAGPELVSARSVEAVQRLVA
HHHHHHHHHEEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
AARRDPVVIRLLAEPGERLDKLAVEAPEFHSAVLAELTLIGHRGPAEVEMAATSYADNPE
HHCCCCEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEHHCCCCCHH
LLVRMVAKTLRAVPAPQPPTPVIPLRAKPVALLAARQLRDREVRRDRMVRAIWVLRALLR
HHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EYGRRLTEAGVFDTPDDVFYLLVDEIDALPADVSGLVARRRAEQRRLAGIVPPTVFSGSW
HHHHHHHHCCCCCCCCHHEEEEEHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCC
EPSPSSAAALAAGDTLRGVGVCGGRVRGRVRIVRPETIDDLQPGEILVAEVTDVGYTAAF
CCCCCCCCEEECCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEEECCCCHHHHH
CYAAAVVTELGGPMSHAAVVAREFGFPCVVDAQGATRFLPPGALVEVDGATGEIHVVELA
HHHHHHHHHHCCCHHHHHHHHHHCCCCEEEECCCCCEECCCCCEEEECCCCCEEEEEEEE
SEDGPALPGSDLSR
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 10.5 {phosphate}} 0.028 {ATP}} 0.083 {pyruvate}} [C]

Substrates: ATP; H2O; Pyruvate [C]

Specific reaction: ATP + H2O + Pyruvate --> AMP + (2) H+ + Phosphoenolpyruvate + Phosphate [C]

General reaction: Phospho group transfer [C]

Inhibitor: 2-Oxoglutarate; 3-Phosphoglyceraldehyde; 5'-Adenylyl methylen ediphosphonate; ADP; ADP glucose; AMP; ATP; Ca2+; F-; Iodoacetate; Malate; Mg2+; Mn2+; Oxalacetate; PCMB; Phosphoenolpyruvate; Sulfhydryl reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036