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The map label for this gene is gidB

Identifier: 148663786

GI number: 148663786

Start: 4415973

End: 4416647

Strand: Reverse

Name: gidB

Synonym: MRA_3958

Alternate gene names: 148663786

Gene position: 4416647-4415973 (Counterclockwise)

Preceding gene: 148663787

Following gene: 148663785

Centisome position: 99.92

GC content: 66.07

Gene sequence:

>675_bases
ATGTCTCCGATCGAGCCCGCGGCGTCTGCGATCTTCGGACCGCGGCTTGGCCTTGCTCGGCGGTACGCCGAAGCGTTGGC
GGGACCCGGTGTGGAGCGGGGGCTGGTGGGACCCCGCGAAGTCGGTAGGCTATGGGACCGGCATCTACTGAACTGCGCCG
TGATCGGTGAGCTCCTCGAACGCGGTGACCGGGTCGTGGATATCGGTAGCGGAGCCGGGTTGCCGGGCGTGCCATTGGCG
ATAGCGCGGCCGGACCTCCAGGTAGTTCTCCTAGAACCGCTACTGCGCCGCACCGAGTTTCTTCGAGAGATGGTGACAGA
TCTGGGCGTGGCCGTTGAGATCGTGCGGGGGCGCGCCGAGGAGTCCTGGGTGCAGGACCAATTGGGCGGCAGCGACGCTG
CGGTGTCACGGGCGGTGGCCGCGTTGGACAAGTTGACGAAATGGAGCATGCCGTTGATACGGCCGAACGGGCGAATGCTC
GCCATCAAAGGCGAGCGGGCTCACGACGAAGTACGGGAGCACCGGCGTGTGATGATCGCATCGGGCGCGGTTGATGTCAG
GGTGGTGACATGTGGCGCGAACTATTTGCGTCCGCCCGCGACCGTGGTGTTCGCACGACGTGGAAAGCAGATCGCCCGAG
GGTCGGCACGGATGGCGAGTGGAGGGACGGCGTGA

Upstream 100 bases:

>100_bases
AGTAAGCGATGCGTGGCCGAGCGGCTGGGCCAGCGTCTCGAGAGCGGAGAATGTTTCACGTGAAACATGACACAGACCTC
ACGAGCCGGCGGAGTGCGTA

Downstream 100 bases:

>100_bases
GTGCTCCGTGGGGCCCGGTGGCCGCTGGACCGTCCGCGCTCGTAAGGTCGGGCCAGGCTTCAACTATCGAACCATTCCAG
CGGGAAATGACACCACCGAC

Product: 16S rRNA methyltransferase GidB

Products: NA

Alternate protein names: 16S rRNA 7-methylguanosine methyltransferase; 16S rRNA m7G methyltransferase

Number of amino acids: Translated: 224; Mature: 223

Protein sequence:

>224_residues
MSPIEPAASAIFGPRLGLARRYAEALAGPGVERGLVGPREVGRLWDRHLLNCAVIGELLERGDRVVDIGSGAGLPGVPLA
IARPDLQVVLLEPLLRRTEFLREMVTDLGVAVEIVRGRAEESWVQDQLGGSDAAVSRAVAALDKLTKWSMPLIRPNGRML
AIKGERAHDEVREHRRVMIASGAVDVRVVTCGANYLRPPATVVFARRGKQIARGSARMASGGTA

Sequences:

>Translated_224_residues
MSPIEPAASAIFGPRLGLARRYAEALAGPGVERGLVGPREVGRLWDRHLLNCAVIGELLERGDRVVDIGSGAGLPGVPLA
IARPDLQVVLLEPLLRRTEFLREMVTDLGVAVEIVRGRAEESWVQDQLGGSDAAVSRAVAALDKLTKWSMPLIRPNGRML
AIKGERAHDEVREHRRVMIASGAVDVRVVTCGANYLRPPATVVFARRGKQIARGSARMASGGTA
>Mature_223_residues
SPIEPAASAIFGPRLGLARRYAEALAGPGVERGLVGPREVGRLWDRHLLNCAVIGELLERGDRVVDIGSGAGLPGVPLAI
ARPDLQVVLLEPLLRRTEFLREMVTDLGVAVEIVRGRAEESWVQDQLGGSDAAVSRAVAALDKLTKWSMPLIRPNGRMLA
IKGERAHDEVREHRRVMIASGAVDVRVVTCGANYLRPPATVVFARRGKQIARGSARMASGGTA

Specific function: Specifically methylates the N7 position of guanosine in position 518 of 16S rRNA

COG id: COG0357

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in bacterial cell division

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA methyltransferase rsmG family

Homologues:

Organism=Escherichia coli, GI1790179, Length=151, Percent_Identity=33.7748344370861, Blast_Score=88, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMG_MYCBO (P59964)

Other databases:

- EMBL:   BX248347
- RefSeq:   NP_857585.1
- ProteinModelPortal:   P59964
- SMR:   P59964
- EnsemblBacteria:   EBMYCT00000017154
- GeneID:   1093956
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb3950c
- GeneTree:   EBGT00050000016057
- HOGENOM:   HBG686577
- OMA:   VPRLWER
- ProtClustDB:   PRK00107
- BioCyc:   MBOV233413:MB3950C-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00074
- InterPro:   IPR003682
- PIRSF:   PIRSF003078
- TIGRFAMs:   TIGR00138

Pfam domain/function: PF02527 GidB

EC number: 2.1.-.-

Molecular weight: Translated: 24032; Mature: 23901

Theoretical pI: Translated: 10.75; Mature: 10.75

Prosite motif: PS00539 PYROKININ

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPIEPAASAIFGPRLGLARRYAEALAGPGVERGLVGPREVGRLWDRHLLNCAVIGELLE
CCCCCHHHHHHHCCHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
RGDRVVDIGSGAGLPGVPLAIARPDLQVVLLEPLLRRTEFLREMVTDLGVAVEIVRGRAE
CCCCEEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
ESWVQDQLGGSDAAVSRAVAALDKLTKWSMPLIRPNGRMLAIKGERAHDEVREHRRVMIA
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEECCCHHHHHHHHHEEEEE
SGAVDVRVVTCGANYLRPPATVVFARRGKQIARGSARMASGGTA
CCCEEEEEEEECCCCCCCCHHHHHHHCCHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
SPIEPAASAIFGPRLGLARRYAEALAGPGVERGLVGPREVGRLWDRHLLNCAVIGELLE
CCCCHHHHHHHCCHHHHHHHHHHHHCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
RGDRVVDIGSGAGLPGVPLAIARPDLQVVLLEPLLRRTEFLREMVTDLGVAVEIVRGRAE
CCCCEEEECCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
ESWVQDQLGGSDAAVSRAVAALDKLTKWSMPLIRPNGRMLAIKGERAHDEVREHRRVMIA
HHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEECCCHHHHHHHHHEEEEE
SGAVDVRVVTCGANYLRPPATVVFARRGKQIARGSARMASGGTA
CCCEEEEEEEECCCCCCCCHHHHHHHCCHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972