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The map label for this gene is espB

Identifier: 148663748

GI number: 148663748

Start: 4368989

End: 4370371

Strand: Reverse

Name: espB

Synonym: MRA_3920

Alternate gene names: 148663748

Gene position: 4370371-4368989 (Counterclockwise)

Preceding gene: 148663749

Following gene: 148663747

Centisome position: 98.88

GC content: 65.08

Gene sequence:

>1383_bases
ATGACGCAGTCGCAGACCGTGACGGTGGATCAGCAAGAGATTTTGAACAGGGCCAACGAGGTGGAGGCCCCGATGGCGGA
CCCACCGACTGATGTCCCCATCACACCGTGCGAACTCACGGCGGCTAAAAACGCCGCCCAACAGCTGGTATTGTCCGCCG
ACAACATGCGGGAATACCTGGCGGCCGGTGCCAAAGAGCGGCAGCGTCTGGCGACCTCGCTGCGCAACGCGGCCAAGGCG
TATGGCGAGGTTGATGAGGAGGCTGCGACCGCGCTGGACAACGACGGCGAAGGAACTGTGCAGGCAGAATCGGCCGGGGC
CGTCGGAGGGGACAGTTCGGCCGAACTAACCGATACGCCGAGGGTGGCCACGGCCGGTGAACCCAACTTCATGGATCTCA
AAGAAGCGGCAAGGAAGCTCGAAACGGGCGACCAAGGCGCATCGCTCGCGCACTTTGCGGATGGGTGGAACACTTTCAAC
CTGACGCTGCAAGGCGACGTCAAGCGGTTCCGGGGGTTTGACAACTGGGAAGGCGATGCGGCTACCGCTTGCGAGGCTTC
GCTCGATCAACAACGGCAATGGATACTCCACATGGCCAAATTGAGCGCTGCGATGGCCAAGCAGGCTCAATATGTCGCGC
AGCTGCACGTGTGGGCTAGGCGGGAACATCCGACTTATGAAGACATAGTCGGGCTCGAACGGCTTTACGCGGAAAACCCT
TCGGCCCGCGACCAAATTCTCCCGGTGTACGCGGAGTATCAGCAGAGGTCGGAGAAGGTGCTGACCGAATACAACAACAA
GGCAGCCCTGGAACCGGTAAACCCGCCGAAGCCTCCCCCCGCCATCAAGATCGACCCGCCCCCGCCTCCGCAAGAGCAGG
GATTGATCCCTGGCTTCCTGATGCCGCCGTCTGACGGCTCCGGTGTGACTCCCGGTACCGGGATGCCAGCCGCACCGATG
GTTCCGCCTACCGGATCGCCGGGTGGTGGCCTCCCGGCTGACACGGCGGCGCAGCTGACGTCGGCTGGGCGGGAAGCCGC
AGCGCTGTCGGGCGACGTGGCGGTCAAAGCGGCATCGCTCGGTGGCGGTGGAGGCGGCGGGGTGCCGTCGGCGCCGTTGG
GATCCGCGATCGGGGGCGCCGAATCGGTGCGGCCCGCTGGCGCTGGTGACATTGCCGGCTTAGGCCAGGGAAGGGCCGGC
GGCGGCGCCGCGCTGGGCGGCGGTGGCATGGGAATGCCGATGGGTGCCGCGCATCAGGGACAAGGGGGCGCCAAGTCCAA
GGGTTCTCAGCAGGAAGACGAGGCGCTCTACACCGAGGATCGGGCATGGACCGAGGCCGTCATTGGTAACCGTCGGCGCC
AGGACAGTAAGGAGTCGAAGTGA

Upstream 100 bases:

>100_bases
CCGTTCGCGATGCCGGCATGAACGGGCGGCATCAAATTAGTGCAGGAACCTTTCAGTTTAGCGACGATAATGGCTATAGC
ACTAAGGAGGATGATCCGAT

Downstream 100 bases:

>100_bases
GCATGGACGAATTGGACCCGCATGTCGCCCGGGCGTTGACGCTGGCGGCGCGGTTTCAGTCGGCCCTAGACGGGACGCTC
AATCAGATGAACAACGGATC

Product: hypothetical protein

Products: NA

Alternate protein names: Antigen MTB48

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MTQSQTVTVDQQEILNRANEVEAPMADPPTDVPITPCELTAAKNAAQQLVLSADNMREYLAAGAKERQRLATSLRNAAKA
YGEVDEEAATALDNDGEGTVQAESAGAVGGDSSAELTDTPRVATAGEPNFMDLKEAARKLETGDQGASLAHFADGWNTFN
LTLQGDVKRFRGFDNWEGDAATACEASLDQQRQWILHMAKLSAAMAKQAQYVAQLHVWARREHPTYEDIVGLERLYAENP
SARDQILPVYAEYQQRSEKVLTEYNNKAALEPVNPPKPPPAIKIDPPPPPQEQGLIPGFLMPPSDGSGVTPGTGMPAAPM
VPPTGSPGGGLPADTAAQLTSAGREAAALSGDVAVKAASLGGGGGGGVPSAPLGSAIGGAESVRPAGAGDIAGLGQGRAG
GGAALGGGGMGMPMGAAHQGQGGAKSKGSQQEDEALYTEDRAWTEAVIGNRRRQDSKESK

Sequences:

>Translated_460_residues
MTQSQTVTVDQQEILNRANEVEAPMADPPTDVPITPCELTAAKNAAQQLVLSADNMREYLAAGAKERQRLATSLRNAAKA
YGEVDEEAATALDNDGEGTVQAESAGAVGGDSSAELTDTPRVATAGEPNFMDLKEAARKLETGDQGASLAHFADGWNTFN
LTLQGDVKRFRGFDNWEGDAATACEASLDQQRQWILHMAKLSAAMAKQAQYVAQLHVWARREHPTYEDIVGLERLYAENP
SARDQILPVYAEYQQRSEKVLTEYNNKAALEPVNPPKPPPAIKIDPPPPPQEQGLIPGFLMPPSDGSGVTPGTGMPAAPM
VPPTGSPGGGLPADTAAQLTSAGREAAALSGDVAVKAASLGGGGGGGVPSAPLGSAIGGAESVRPAGAGDIAGLGQGRAG
GGAALGGGGMGMPMGAAHQGQGGAKSKGSQQEDEALYTEDRAWTEAVIGNRRRQDSKESK
>Mature_459_residues
TQSQTVTVDQQEILNRANEVEAPMADPPTDVPITPCELTAAKNAAQQLVLSADNMREYLAAGAKERQRLATSLRNAAKAY
GEVDEEAATALDNDGEGTVQAESAGAVGGDSSAELTDTPRVATAGEPNFMDLKEAARKLETGDQGASLAHFADGWNTFNL
TLQGDVKRFRGFDNWEGDAATACEASLDQQRQWILHMAKLSAAMAKQAQYVAQLHVWARREHPTYEDIVGLERLYAENPS
ARDQILPVYAEYQQRSEKVLTEYNNKAALEPVNPPKPPPAIKIDPPPPPQEQGLIPGFLMPPSDGSGVTPGTGMPAAPMV
PPTGSPGGGLPADTAAQLTSAGREAAALSGDVAVKAASLGGGGGGGVPSAPLGSAIGGAESVRPAGAGDIAGLGQGRAGG
GAALGGGGMGMPMGAAHQGQGGAKSKGSQQEDEALYTEDRAWTEAVIGNRRRQDSKESK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted. Note=Secreted via the ESX-1 / type VII secretion system (T7SS). Secretion requires both EspK and EccCb1

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ESPB_MYCTU (Q933K8)

Other databases:

- EMBL:   AY029285
- EMBL:   BX842584
- EMBL:   AE000516
- PIR:   G70803
- RefSeq:   NP_218398.1
- RefSeq:   NP_338550.1
- EnsemblBacteria:   EBMYCT00000002870
- EnsemblBacteria:   EBMYCT00000071071
- GeneID:   886214
- GeneID:   922535
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT3996
- KEGG:   mtu:Rv3881c
- TIGR:   MT3996
- TubercuList:   Rv3881c
- GeneTree:   EBGT00050000014870
- HOGENOM:   HBG569801
- OMA:   HECESTR
- ProtClustDB:   CLSK792783
- GO:   GO:0009405

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 47594; Mature: 47463

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQSQTVTVDQQEILNRANEVEAPMADPPTDVPITPCELTAAKNAAQQLVLSADNMREYL
CCCCCEEEECHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AAGAKERQRLATSLRNAAKAYGEVDEEAATALDNDGEGTVQAESAGAVGGDSSAELTDTP
HCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCC
RVATAGEPNFMDLKEAARKLETGDQGASLAHFADGWNTFNLTLQGDVKRFRGFDNWEGDA
CEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHCCCEEEEEEEECCHHHHCCCCCCCCCH
ATACEASLDQQRQWILHMAKLSAAMAKQAQYVAQLHVWARREHPTYEDIVGLERLYAENP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
SARDQILPVYAEYQQRSEKVLTEYNNKAALEPVNPPKPPPAIKIDPPPPPQEQGLIPGFL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCEE
MPPSDGSGVTPGTGMPAAPMVPPTGSPGGGLPADTAAQLTSAGREAAALSGDVAVKAASL
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHCCCCEEEEEECC
GGGGGGGVPSAPLGSAIGGAESVRPAGAGDIAGLGQGRAGGGAALGGGGMGMPMGAAHQG
CCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCC
QGGAKSKGSQQEDEALYTEDRAWTEAVIGNRRRQDSKESK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHCCCCCCC
>Mature Secondary Structure 
TQSQTVTVDQQEILNRANEVEAPMADPPTDVPITPCELTAAKNAAQQLVLSADNMREYL
CCCCEEEECHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AAGAKERQRLATSLRNAAKAYGEVDEEAATALDNDGEGTVQAESAGAVGGDSSAELTDTP
HCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCC
RVATAGEPNFMDLKEAARKLETGDQGASLAHFADGWNTFNLTLQGDVKRFRGFDNWEGDA
CEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHCCCEEEEEEEECCHHHHCCCCCCCCCH
ATACEASLDQQRQWILHMAKLSAAMAKQAQYVAQLHVWARREHPTYEDIVGLERLYAENP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
SARDQILPVYAEYQQRSEKVLTEYNNKAALEPVNPPKPPPAIKIDPPPPPQEQGLIPGFL
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCEE
MPPSDGSGVTPGTGMPAAPMVPPTGSPGGGLPADTAAQLTSAGREAAALSGDVAVKAASL
ECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHCCCCEEEEEECC
GGGGGGGVPSAPLGSAIGGAESVRPAGAGDIAGLGQGRAGGGAALGGGGMGMPMGAAHQG
CCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCC
QGGAKSKGSQQEDEALYTEDRAWTEAVIGNRRRQDSKESK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427558; 9634230; 12218036