Click here to switch to the map view.

The map label for this gene is 148663606

Identifier: 148663606

GI number: 148663606

Start: 4199279

End: 4200625

Strand: Reverse

Name: 148663606

Synonym: MRA_3778

Alternate gene names: NA

Gene position: 4200625-4199279 (Counterclockwise)

Preceding gene: 148663607

Following gene: 148663605

Centisome position: 95.04

GC content: 65.4

Gene sequence:

>1347_bases
ATGTCACCGATCGATGCGCTGTTCTTATCAGCCGAGTCGCGAGAGCATCCGCTGCATGTCGGCGCGCTGCAACTGTTCGA
GCCACCCGCGGGGGCGGGACGTGGGTTTGTGCGGGAAACCTATCAGGCGATGCTTCAGTGCCGCGAGATAGCACCGCTAT
TTCGCAAGCGCCCCACGTCGCTTCACGGTGCACTCATCAACCTCGGCTGGTCGACCGACGCTGACGTCGACCTCGGCTAC
CACGCGCGGCGGTCTGCCCTGCCGGCGCCGGGCCGGGTGCGTGAGCTGCTGGAACTGACGTCACGGTTACACTCCAATCT
GCTCGACCGGCACCGCCCGCTCTGGGAAACACACGTGATCGAGGGCCTCCGGGATGGACGCTTTGCGATCTATTCGAAGA
TGCACCACGCCCTGGTCGACGGGGTGTCCGGACTTACGCTGATGCGCCAGCCGATGACCACCGACCCCATCGAGGGCAAG
TTGCGCACCGCCTGGTCACCGGCGACGCAGCACACCGCCATCAAGCGACGGCGCGGCCGCCTGCAACAGCTGGGGGGCAT
GCTGGGATCGGTTGCCGGGCTCGCTCCCTCAACGTTGCGACTGGCGCGTTCCGCGTTGATCGAACAACAGTTGACACTGC
CCTTCGGGGCCCCGCACACCATGCTCAATGTCGCCGTCGGGGGAGCGCGGCGCTGCGCCGCGCAGTCCTGGCCGTTGGAC
CGGGTCAAGGCGGTGAAAGACGCGGCCGGGGTCAGCCTCAACGACGTGGTGCTGGCGATGTGCGCCGGCGCACTTCGCGA
ATATCTGGACGACAACGACGCGCTGCCGGACACGCCGCTGGTTGCGATGGTTCCGGTGAGCCTGCGCACCGACCGCGACT
CGGTCGGCGGCAACATGGTCGGGGCGGTGTTGTGTAATCTGGCCACCCACCTTGATGATCCAGCCGACCGGCTGAACGCC
ATCCACGCTTCGATGCGCGGTAATAAGAACGTACTATCGCAGCTGCCCCGCGCCCAGGCGTTGGCGGTGTCACTGCTGCT
GTTGAGCCCAGCTGCGCTGAACACCCTGCCGGGCTTGGCCAAGGCAACGCCACCGCCGTTTAATGTGTGCATCTCGAACG
TGCCCGGTGCGCGCGAACCGCTGTACTTCAACGGCGCCAGGATGGTCGGCAACTATCCAATGTCGTTGGTGCTCGACGGA
CAAGCGCTCAACATCACCCTGACCAGCACCGCCGATAGTCTCGACTTCGGAGTCGTGGGTTGCCGCCGCAGCGTTCCACA
CGTGCAGCGGGTACTCAGCCATCTGGAGACCTCGTTGAAGGAACTGGAGCGCGCGGTCGGGCTATGA

Upstream 100 bases:

>100_bases
GCGAGGGCCCGGTCGACGACGATCACCTGCGCTTTACGACGTCGGCTTCGGCGAGCCGGCTGACGGTGAAGCGCATCACC
AGGAGCACGCCATGGAACTG

Downstream 100 bases:

>100_bases
GGAAGTGCTGCGGGTCGCGCTGGGTTTGACCAAAGCCAGCCCAGTTTATCTGCCTGGTGTAAAGGGACGTATCTTGCTGG
CTGGGCAACTCGAGCATCGT

Product: hypothetical protein

Products: NA

Alternate protein names: Putative triacylglycerol synthase Rv3740c/MT3848

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MSPIDALFLSAESREHPLHVGALQLFEPPAGAGRGFVRETYQAMLQCREIAPLFRKRPTSLHGALINLGWSTDADVDLGY
HARRSALPAPGRVRELLELTSRLHSNLLDRHRPLWETHVIEGLRDGRFAIYSKMHHALVDGVSGLTLMRQPMTTDPIEGK
LRTAWSPATQHTAIKRRRGRLQQLGGMLGSVAGLAPSTLRLARSALIEQQLTLPFGAPHTMLNVAVGGARRCAAQSWPLD
RVKAVKDAAGVSLNDVVLAMCAGALREYLDDNDALPDTPLVAMVPVSLRTDRDSVGGNMVGAVLCNLATHLDDPADRLNA
IHASMRGNKNVLSQLPRAQALAVSLLLLSPAALNTLPGLAKATPPPFNVCISNVPGAREPLYFNGARMVGNYPMSLVLDG
QALNITLTSTADSLDFGVVGCRRSVPHVQRVLSHLETSLKELERAVGL

Sequences:

>Translated_448_residues
MSPIDALFLSAESREHPLHVGALQLFEPPAGAGRGFVRETYQAMLQCREIAPLFRKRPTSLHGALINLGWSTDADVDLGY
HARRSALPAPGRVRELLELTSRLHSNLLDRHRPLWETHVIEGLRDGRFAIYSKMHHALVDGVSGLTLMRQPMTTDPIEGK
LRTAWSPATQHTAIKRRRGRLQQLGGMLGSVAGLAPSTLRLARSALIEQQLTLPFGAPHTMLNVAVGGARRCAAQSWPLD
RVKAVKDAAGVSLNDVVLAMCAGALREYLDDNDALPDTPLVAMVPVSLRTDRDSVGGNMVGAVLCNLATHLDDPADRLNA
IHASMRGNKNVLSQLPRAQALAVSLLLLSPAALNTLPGLAKATPPPFNVCISNVPGAREPLYFNGARMVGNYPMSLVLDG
QALNITLTSTADSLDFGVVGCRRSVPHVQRVLSHLETSLKELERAVGL
>Mature_447_residues
SPIDALFLSAESREHPLHVGALQLFEPPAGAGRGFVRETYQAMLQCREIAPLFRKRPTSLHGALINLGWSTDADVDLGYH
ARRSALPAPGRVRELLELTSRLHSNLLDRHRPLWETHVIEGLRDGRFAIYSKMHHALVDGVSGLTLMRQPMTTDPIEGKL
RTAWSPATQHTAIKRRRGRLQQLGGMLGSVAGLAPSTLRLARSALIEQQLTLPFGAPHTMLNVAVGGARRCAAQSWPLDR
VKAVKDAAGVSLNDVVLAMCAGALREYLDDNDALPDTPLVAMVPVSLRTDRDSVGGNMVGAVLCNLATHLDDPADRLNAI
HASMRGNKNVLSQLPRAQALAVSLLLLSPAALNTLPGLAKATPPPFNVCISNVPGAREPLYFNGARMVGNYPMSLVLDGQ
ALNITLTSTADSLDFGVVGCRRSVPHVQRVLSHLETSLKELERAVGL

Specific function: Upon expression in E.coli has a weak triacylglycerol synthase function, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Also functions weakly as a wax synthase, as it incorporates palmityl alcohol into wax esters in the presence of

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y3740_MYCTU (O69707)

Other databases:

- EMBL:   BX842584
- EMBL:   AE000516
- PIR:   E70798
- RefSeq:   NP_218257.1
- RefSeq:   NP_338398.1
- ProteinModelPortal:   O69707
- EnsemblBacteria:   EBMYCT00000003535
- EnsemblBacteria:   EBMYCT00000070767
- GeneID:   885781
- GeneID:   922659
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT3848
- KEGG:   mtu:Rv3740c
- TIGR:   MT3848
- TubercuList:   Rv3740c
- GeneTree:   EBGT00050000015226
- HOGENOM:   HBG696081
- OMA:   YLEDNDA
- ProtClustDB:   CLSK2748676
- InterPro:   IPR014292
- InterPro:   IPR009721
- InterPro:   IPR004255
- TIGRFAMs:   TIGR02946

Pfam domain/function: PF06974 DUF1298; PF03007 UPF0089

EC number: =2.3.1.20

Molecular weight: Translated: 48354; Mature: 48222

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: NA

Important sites: ACT_SITE 136-136

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPIDALFLSAESREHPLHVGALQLFEPPAGAGRGFVRETYQAMLQCREIAPLFRKRPTS
CCCHHHHHCCCCCCCCCEEECHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
LHGALINLGWSTDADVDLGYHARRSALPAPGRVRELLELTSRLHSNLLDRHRPLWETHVI
CCCCEEECCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
EGLRDGRFAIYSKMHHALVDGVSGLTLMRQPMTTDPIEGKLRTAWSPATQHTAIKRRRGR
HHHCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
LQQLGGMLGSVAGLAPSTLRLARSALIEQQLTLPFGAPHTMLNVAVGGARRCAAQSWPLD
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHCCCCHH
RVKAVKDAAGVSLNDVVLAMCAGALREYLDDNDALPDTPLVAMVPVSLRTDRDSVGGNMV
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCHHCCCHHH
GAVLCNLATHLDDPADRLNAIHASMRGNKNVLSQLPRAQALAVSLLLLSPAALNTLPGLA
HHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHCCHHHHHCCCCC
KATPPPFNVCISNVPGAREPLYFNGARMVGNYPMSLVLDGQALNITLTSTADSLDFGVVG
CCCCCCHHHHCCCCCCCCCCEEECCCEEECCCCEEEEECCCEEEEEEECCCCCCCHHHHH
CRRSVPHVQRVLSHLETSLKELERAVGL
HHCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SPIDALFLSAESREHPLHVGALQLFEPPAGAGRGFVRETYQAMLQCREIAPLFRKRPTS
CCHHHHHCCCCCCCCCEEECHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
LHGALINLGWSTDADVDLGYHARRSALPAPGRVRELLELTSRLHSNLLDRHRPLWETHVI
CCCCEEECCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
EGLRDGRFAIYSKMHHALVDGVSGLTLMRQPMTTDPIEGKLRTAWSPATQHTAIKRRRGR
HHHCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
LQQLGGMLGSVAGLAPSTLRLARSALIEQQLTLPFGAPHTMLNVAVGGARRCAAQSWPLD
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHCCCCHH
RVKAVKDAAGVSLNDVVLAMCAGALREYLDDNDALPDTPLVAMVPVSLRTDRDSVGGNMV
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCHHCCCHHH
GAVLCNLATHLDDPADRLNAIHASMRGNKNVLSQLPRAQALAVSLLLLSPAALNTLPGLA
HHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHCCHHHHHCCCCC
KATPPPFNVCISNVPGAREPLYFNGARMVGNYPMSLVLDGQALNITLTSTADSLDFGVVG
CCCCCCHHHHCCCCCCCCCCEEECCCEEECCCCEEEEECCCEEEEEEECCCCCCCHHHHH
CRRSVPHVQRVLSHLETSLKELERAVGL
HHCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036