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The map label for this gene is tgs2

Identifier: 148663600

GI number: 148663600

Start: 4192972

End: 4194336

Strand: Reverse

Name: tgs2

Synonym: MRA_3772

Alternate gene names: 148663600

Gene position: 4194336-4192972 (Counterclockwise)

Preceding gene: 148663604

Following gene: 148663599

Centisome position: 94.89

GC content: 62.27

Gene sequence:

>1365_bases
ATGGATCTGATGATGCCCAACGACTCGATGTTTCTTTTCATTGAATCTCGTGAACACCCCATGCATGTGGGTGGCTTGTC
GTTGTTCGAGCCACCGCAGGGAGCTGGTCCTGAGTTCGTCCGTGAGTTCACCGAGCGTTTGGTTGCCAACGACGAATTCC
AGCCCATGTTCCGAAAGCACCCTGCGACGATCGGGGGTGGAATCGCCCGTGTGGCATGGGCTTATGACGACGACATCGAC
ATCGATTACCACGTTCGCCGCTCGGCGCTGCCCTCGCCAGGACGAGTCCGCGATCTGCTCGAGCTGACCTCAAGGCTGCA
TACCAGCCTGCTCGATCGCCACCGCCCGCTGTGGGAGCTGCACGTGGTGGAGGGGCTGAACGACGGCCGGTTTGCTATGT
ACACCAAAATGCACCACGCCCTGATCGACGGTGTTTCGGCGATGAAGCTGGCGCAGCGCACGCTGTCGGCTGACCCGGAC
GACGCCGAGGTGCGCGCCATCTGGAACCTGCCTCCGCGCCCACGTACGCGCCCGCCATCGGACGGCTCGTCACTGTTGGA
TGCGCTGTTCAAGATGGCGGGATCCGTTGTCGGACTTGCACCGTCGACGCTGAAGCTGGCTCGTGCGGCGCTACTCGAGC
AGCAGTTGACGCTGCCGTTCGCGGCGCCACACAGCATGTTCAACGTCAAGGTCGGGGGAGCCCGCCGGTGCGCTGCGCAG
TCCTGGTCGCTGGACCGGATCAAGAGCGTCAAGCAGGCCGCCGGTGTGACCGTCAACGACGCCGTCCTGGCGATGTGTGC
CGGTGCGTTGCGCTACTACCTGATAGAGCGGAATGCGCTGCCGGACAGACCGCTGATCGCGATGGTTCCGGTGAGCCTAC
GCTCCAAAGAGGACGCTGACGCCGGCGGCAACTTGGTCGGCAGCGTCCTGTGCAATCTCGCCACCCATGTCGACGACCCA
GCGCAACGGATTCAGACCATCAGCGCGTCCATGGACGGCAACAAGAAAGTGCTCTCCGAATTGCCGCAGCTGCAGGTGCT
TGCATTGTCGGCATTGAATATGGCGCCGCTGACGTTGGCGGGTGTACCGGGTTTCTTGTCGGCAGTGCCGCCACCGTTCA
ACATCGTCATCTCGAATGTGCCGGGGCCGGTAGACCCGCTCTACTACGGCACGGCCCGGCTTGACGGTAGTTACCCATTG
TCGAACATCCCGGACGGTCAAGCGTTGAACATCACCCTGGTGAACAATGCTGGGAATCTCGACTTCGGACTGGTCGGGTG
CCGTCGCAGTGTGCCCCACTTGCAGCGGCTGCTTGCGCACCTGGAATCGTCGCTGAAGGATCTGGAACAGGCCGTCGGGA
TCTAA

Upstream 100 bases:

>100_bases
CATCCCGAAGTCAGCTGGGCGGCGATGTTCTTGCGACTTGCCGAACCTCGGGCACCACAGAAGCCTTCCCGGATCACTCG
GTAATGGGTGTAATGGGTGC

Downstream 100 bases:

>100_bases
AGAACCCGTACTCATGCCCAAGCTCAGCGCGGGTGTGCTGCTGTATCGGGCGCGCGCCGGTGTCGTCGACGTCCTTCTGG
CGCATCCGGGCGGCCCGTTT

Product: hypothetical protein

Products: NA

Alternate protein names: TGS2; Diacylglycerol O-acyltransferase; DGAT; Long-chain-alcohol O-fatty-acyltransferase; Probable triacylglycerol synthase tgs2; Wax ester synthase/acyl-CoA:diacylglycerol acyltransferase; Wax synthase; WS

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
MDLMMPNDSMFLFIESREHPMHVGGLSLFEPPQGAGPEFVREFTERLVANDEFQPMFRKHPATIGGGIARVAWAYDDDID
IDYHVRRSALPSPGRVRDLLELTSRLHTSLLDRHRPLWELHVVEGLNDGRFAMYTKMHHALIDGVSAMKLAQRTLSADPD
DAEVRAIWNLPPRPRTRPPSDGSSLLDALFKMAGSVVGLAPSTLKLARAALLEQQLTLPFAAPHSMFNVKVGGARRCAAQ
SWSLDRIKSVKQAAGVTVNDAVLAMCAGALRYYLIERNALPDRPLIAMVPVSLRSKEDADAGGNLVGSVLCNLATHVDDP
AQRIQTISASMDGNKKVLSELPQLQVLALSALNMAPLTLAGVPGFLSAVPPPFNIVISNVPGPVDPLYYGTARLDGSYPL
SNIPDGQALNITLVNNAGNLDFGLVGCRRSVPHLQRLLAHLESSLKDLEQAVGI

Sequences:

>Translated_454_residues
MDLMMPNDSMFLFIESREHPMHVGGLSLFEPPQGAGPEFVREFTERLVANDEFQPMFRKHPATIGGGIARVAWAYDDDID
IDYHVRRSALPSPGRVRDLLELTSRLHTSLLDRHRPLWELHVVEGLNDGRFAMYTKMHHALIDGVSAMKLAQRTLSADPD
DAEVRAIWNLPPRPRTRPPSDGSSLLDALFKMAGSVVGLAPSTLKLARAALLEQQLTLPFAAPHSMFNVKVGGARRCAAQ
SWSLDRIKSVKQAAGVTVNDAVLAMCAGALRYYLIERNALPDRPLIAMVPVSLRSKEDADAGGNLVGSVLCNLATHVDDP
AQRIQTISASMDGNKKVLSELPQLQVLALSALNMAPLTLAGVPGFLSAVPPPFNIVISNVPGPVDPLYYGTARLDGSYPL
SNIPDGQALNITLVNNAGNLDFGLVGCRRSVPHLQRLLAHLESSLKDLEQAVGI
>Mature_454_residues
MDLMMPNDSMFLFIESREHPMHVGGLSLFEPPQGAGPEFVREFTERLVANDEFQPMFRKHPATIGGGIARVAWAYDDDID
IDYHVRRSALPSPGRVRDLLELTSRLHTSLLDRHRPLWELHVVEGLNDGRFAMYTKMHHALIDGVSAMKLAQRTLSADPD
DAEVRAIWNLPPRPRTRPPSDGSSLLDALFKMAGSVVGLAPSTLKLARAALLEQQLTLPFAAPHSMFNVKVGGARRCAAQ
SWSLDRIKSVKQAAGVTVNDAVLAMCAGALRYYLIERNALPDRPLIAMVPVSLRSKEDADAGGNLVGSVLCNLATHVDDP
AQRIQTISASMDGNKKVLSELPQLQVLALSALNMAPLTLAGVPGFLSAVPPPFNIVISNVPGPVDPLYYGTARLDGSYPL
SNIPDGQALNITLVNNAGNLDFGLVGCRRSVPHLQRLLAHLESSLKDLEQAVGI

Specific function: Upon expression in E.coli functions as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Also functions as a wax synthase, incorporating palmityl alcohol into wax esters in the presence of palmitoyl-CoA

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGS2_MYCTU (P67210)

Other databases:

- EMBL:   BX842584
- EMBL:   AE000516
- PIR:   G70797
- RefSeq:   NP_218251.1
- RefSeq:   NP_338392.1
- ProteinModelPortal:   P67210
- EnsemblBacteria:   EBMYCT00000003517
- EnsemblBacteria:   EBMYCT00000071309
- GeneID:   885335
- GeneID:   926425
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT3839
- KEGG:   mtu:Rv3734c
- TIGR:   MT3839
- TubercuList:   Rv3734c
- GeneTree:   EBGT00050000015226
- HOGENOM:   HBG696081
- OMA:   APRTMLN
- ProtClustDB:   CLSK792683
- InterPro:   IPR014292
- InterPro:   IPR004255
- TIGRFAMs:   TIGR02946

Pfam domain/function: PF03007 UPF0089

EC number: =2.3.1.20; =2.3.1.75

Molecular weight: Translated: 49304; Mature: 49304

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: ACT_SITE 139-139

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLMMPNDSMFLFIESREHPMHVGGLSLFEPPQGAGPEFVREFTERLVANDEFQPMFRKH
CCCCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHC
PATIGGGIARVAWAYDDDIDIDYHVRRSALPSPGRVRDLLELTSRLHTSLLDRHRPLWEL
CCCCCCCHHEEEEECCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
HVVEGLNDGRFAMYTKMHHALIDGVSAMKLAQRTLSADPDDAEVRAIWNLPPRPRTRPPS
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCC
DGSSLLDALFKMAGSVVGLAPSTLKLARAALLEQQLTLPFAAPHSMFNVKVGGARRCAAQ
CHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHC
SWSLDRIKSVKQAAGVTVNDAVLAMCAGALRYYLIERNALPDRPLIAMVPVSLRSKEDAD
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHCCCCCCC
AGGNLVGSVLCNLATHVDDPAQRIQTISASMDGNKKVLSELPQLQVLALSALNMAPLTLA
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHCCCCEEEE
GVPGFLSAVPPPFNIVISNVPGPVDPLYYGTARLDGSYPLSNIPDGQALNITLVNNAGNL
CCCHHHHCCCCCCEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEEECCCCC
DFGLVGCRRSVPHLQRLLAHLESSLKDLEQAVGI
CEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDLMMPNDSMFLFIESREHPMHVGGLSLFEPPQGAGPEFVREFTERLVANDEFQPMFRKH
CCCCCCCCCEEEEEECCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHC
PATIGGGIARVAWAYDDDIDIDYHVRRSALPSPGRVRDLLELTSRLHTSLLDRHRPLWEL
CCCCCCCHHEEEEECCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEE
HVVEGLNDGRFAMYTKMHHALIDGVSAMKLAQRTLSADPDDAEVRAIWNLPPRPRTRPPS
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCC
DGSSLLDALFKMAGSVVGLAPSTLKLARAALLEQQLTLPFAAPHSMFNVKVGGARRCAAQ
CHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHC
SWSLDRIKSVKQAAGVTVNDAVLAMCAGALRYYLIERNALPDRPLIAMVPVSLRSKEDAD
CCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHCCCCCCC
AGGNLVGSVLCNLATHVDDPAQRIQTISASMDGNKKVLSELPQLQVLALSALNMAPLTLA
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHCCCCEEEE
GVPGFLSAVPPPFNIVISNVPGPVDPLYYGTARLDGSYPLSNIPDGQALNITLVNNAGNL
CCCHHHHCCCCCCEEEEECCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEEEECCCCC
DFGLVGCRRSVPHLQRLLAHLESSLKDLEQAVGI
CEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036