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The map label for this gene is ask

Identifier: 148663574

GI number: 148663574

Start: 4160664

End: 4161929

Strand: Reverse

Name: ask

Synonym: MRA_3746

Alternate gene names: 148663574

Gene position: 4161929-4160664 (Counterclockwise)

Preceding gene: 148663576

Following gene: 148663573

Centisome position: 94.16

GC content: 66.03

Gene sequence:

>1266_bases
GTGGCGCTCGTCGTGCAGAAGTACGGCGGATCCTCGGTGGCCGACGCCGAACGGATTCGCCGCGTCGCCGAACGCATCGT
CGCCACCAAGAAGCAAGGCAATGACGTCGTCGTCGTCGTCTCTGCCATGGGGGATACCACCGACGACCTGCTGGATCTGG
CTCAGCAGGTGTGCCCGGCGCCGCCGCCTCGGGAGCTGGACATGCTGCTTACCGCCGGTGAACGCATCTCGAATGCGTTG
GTGGCCATGGCCATCGAGTCGCTCGGCGCGCATGCCCGGTCGTTCACCGGTTCGCAGGCCGGGGTGATCACCACCGGCAC
CCACGGCAACGCCAAGATCATCGACGTCACGCCGGGGCGGCTGCAAACCGCCCTTGAGGAGGGGCGGGTCGTTTTGGTGG
CCGGATTCCAAGGGGTCAGCCAGGACACCAAGGATGTCACGACGTTGGGCCGCGGCGGCTCGGACACCACCGCCGTCGCC
ATGGCCGCCGCGCTGGGTGCCGATGTCTGTGAGATCTACACCGACGTGGACGGCATCTTCAGCGCCGACCCGCGCATCGT
GCGCAACGCCCGAAAGCTCGACACCGTGACCTTCGAGGAAATGCTCGAGATGGCGGCCTGCGGCGCCAAGGTGCTGATGC
TGCGCTGCGTGGAATACGCTCGCCGCCATAATATTCCGGTGCACGTCCGGTCGTCGTACTCGGACAGACCGGGCACCGTC
GTTGTCGGATCGATCAAGGACGTACCCATGGAAGACCCCATCCTGACCGGAGTCGCGCACGACCGCAGCGAGGCCAAGGT
GACCATCGTCGGGCTGCCCGACATCCCCGGGTATGCGGCCAAGGTGTTTAGGGCGGTGGCCGACGCCGACGTCAACATCG
ACATGGTGCTGCAGAACGTCTCCAAGGTCGAGGACGGCAAGACCGACATCACCTTCACCTGCTCCCGCGACGTCGGGCCC
GCCGCCGTGGAAAAACTGGACTCGCTCAGAAACGAGATCGGCTTCTCACAGCTGCTGTACGACGACCACATCGGCAAGGT
ATCGCTGATCGGTGCCGGCATGCGCAGCCACCCCGGGGTCACCGCGACGTTCTGTGAGGCGCTGGCGGCGGTGGGGGTCA
ACATCGAGCTGATCTCCACCTCGGAGATCAGGATCTCGGTGTTGTGCCGCGACACCGAACTGGACAAGGCCGTGGTCGCG
CTGCATGAAGCGTTCGGGCTCGGCGGCGACGAGGAGGCCACGGTGTACGCGGGGACGGGACGGTAG

Upstream 100 bases:

>100_bases
GTGGGCTACTCGCACATTCCCGTGGTGGACGCAGAGTCAGCGCAGGCCGGCGCGCCCCCTATTCTGGACCTGTCTCCAAT
CTTCCCCGGAAAGGGAAACA

Downstream 100 bases:

>100_bases
ATGGGCCTGTCAATAGGGATCGTGGGGGCCACCGGTCAGGTGGGTCAGGTCATGCGCACGTTGCTCGACGAGCGGGATTT
CCCGGCGAGCGCGGTGCGGT

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase; ASK

Number of amino acids: Translated: 421; Mature: 420

Protein sequence:

>421_residues
MALVVQKYGGSSVADAERIRRVAERIVATKKQGNDVVVVVSAMGDTTDDLLDLAQQVCPAPPPRELDMLLTAGERISNAL
VAMAIESLGAHARSFTGSQAGVITTGTHGNAKIIDVTPGRLQTALEEGRVVLVAGFQGVSQDTKDVTTLGRGGSDTTAVA
MAAALGADVCEIYTDVDGIFSADPRIVRNARKLDTVTFEEMLEMAACGAKVLMLRCVEYARRHNIPVHVRSSYSDRPGTV
VVGSIKDVPMEDPILTGVAHDRSEAKVTIVGLPDIPGYAAKVFRAVADADVNIDMVLQNVSKVEDGKTDITFTCSRDVGP
AAVEKLDSLRNEIGFSQLLYDDHIGKVSLIGAGMRSHPGVTATFCEALAAVGVNIELISTSEIRISVLCRDTELDKAVVA
LHEAFGLGGDEEATVYAGTGR

Sequences:

>Translated_421_residues
MALVVQKYGGSSVADAERIRRVAERIVATKKQGNDVVVVVSAMGDTTDDLLDLAQQVCPAPPPRELDMLLTAGERISNAL
VAMAIESLGAHARSFTGSQAGVITTGTHGNAKIIDVTPGRLQTALEEGRVVLVAGFQGVSQDTKDVTTLGRGGSDTTAVA
MAAALGADVCEIYTDVDGIFSADPRIVRNARKLDTVTFEEMLEMAACGAKVLMLRCVEYARRHNIPVHVRSSYSDRPGTV
VVGSIKDVPMEDPILTGVAHDRSEAKVTIVGLPDIPGYAAKVFRAVADADVNIDMVLQNVSKVEDGKTDITFTCSRDVGP
AAVEKLDSLRNEIGFSQLLYDDHIGKVSLIGAGMRSHPGVTATFCEALAAVGVNIELISTSEIRISVLCRDTELDKAVVA
LHEAFGLGGDEEATVYAGTGR
>Mature_420_residues
ALVVQKYGGSSVADAERIRRVAERIVATKKQGNDVVVVVSAMGDTTDDLLDLAQQVCPAPPPRELDMLLTAGERISNALV
AMAIESLGAHARSFTGSQAGVITTGTHGNAKIIDVTPGRLQTALEEGRVVLVAGFQGVSQDTKDVTTLGRGGSDTTAVAM
AAALGADVCEIYTDVDGIFSADPRIVRNARKLDTVTFEEMLEMAACGAKVLMLRCVEYARRHNIPVHVRSSYSDRPGTVV
VGSIKDVPMEDPILTGVAHDRSEAKVTIVGLPDIPGYAAKVFRAVADADVNIDMVLQNVSKVEDGKTDITFTCSRDVGPA
AVEKLDSLRNEIGFSQLLYDDHIGKVSLIGAGMRSHPGVTATFCEALAAVGVNIELISTSEIRISVLCRDTELDKAVVAL
HEAFGLGGDEEATVYAGTGR

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains

Homologues:

Organism=Escherichia coli, GI1786183, Length=463, Percent_Identity=26.7818574514039, Blast_Score=128, Evalue=7e-31,
Organism=Escherichia coli, GI1790455, Length=348, Percent_Identity=29.8850574712644, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790376, Length=459, Percent_Identity=25.4901960784314, Blast_Score=75, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6320893, Length=376, Percent_Identity=25.7978723404255, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AK_MYCBO (P0A4Z9)

Other databases:

- EMBL:   BX248347
- EMBL:   Z18290
- PIR:   S42425
- RefSeq:   NP_857374.1
- ProteinModelPortal:   P0A4Z9
- SMR:   P0A4Z9
- EnsemblBacteria:   EBMYCT00000014537
- GeneID:   1093392
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb3736c
- GeneTree:   EBGT00050000015976
- HOGENOM:   HBG724395
- OMA:   NKSNRLT
- ProtClustDB:   PRK06635
- BioCyc:   MBOV233413:MB3736C-MONOMER
- BRENDA:   2.7.2.4
- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042
- Gene3D:   G3DSA:3.40.1160.10
- TIGRFAMs:   TIGR00656
- TIGRFAMs:   TIGR00657

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT; SSF53633 Aa_kinase

EC number: =2.7.2.4

Molecular weight: Translated: 44462; Mature: 44331

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALVVQKYGGSSVADAERIRRVAERIVATKKQGNDVVVVVSAMGDTTDDLLDLAQQVCPA
CEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHCCC
PPPRELDMLLTAGERISNALVAMAIESLGAHARSFTGSQAGVITTGTHGNAKIIDVTPGR
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEECCHH
LQTALEEGRVVLVAGFQGVSQDTKDVTTLGRGGSDTTAVAMAAALGADVCEIYTDVDGIF
HHHHHHCCCEEEEECCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
SADPRIVRNARKLDTVTFEEMLEMAACGAKVLMLRCVEYARRHNIPVHVRSSYSDRPGTV
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEE
VVGSIKDVPMEDPILTGVAHDRSEAKVTIVGLPDIPGYAAKVFRAVADADVNIDMVLQNV
EEECCCCCCCCCCEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHH
SKVEDGKTDITFTCSRDVGPAAVEKLDSLRNEIGFSQLLYDDHIGKVSLIGAGMRSHPGV
HHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCHHHHHHHCCCCEEEEEECCCCCCCCC
TATFCEALAAVGVNIELISTSEIRISVLCRDTELDKAVVALHEAFGLGGDEEATVYAGTG
HHHHHHHHHHCCCEEEEEECCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCEEEEECCC
R
C
>Mature Secondary Structure 
ALVVQKYGGSSVADAERIRRVAERIVATKKQGNDVVVVVSAMGDTTDDLLDLAQQVCPA
EEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHCCC
PPPRELDMLLTAGERISNALVAMAIESLGAHARSFTGSQAGVITTGTHGNAKIIDVTPGR
CCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEECCHH
LQTALEEGRVVLVAGFQGVSQDTKDVTTLGRGGSDTTAVAMAAALGADVCEIYTDVDGIF
HHHHHHCCCEEEEECCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCC
SADPRIVRNARKLDTVTFEEMLEMAACGAKVLMLRCVEYARRHNIPVHVRSSYSDRPGTV
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEE
VVGSIKDVPMEDPILTGVAHDRSEAKVTIVGLPDIPGYAAKVFRAVADADVNIDMVLQNV
EEECCCCCCCCCCEEECCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHH
SKVEDGKTDITFTCSRDVGPAAVEKLDSLRNEIGFSQLLYDDHIGKVSLIGAGMRSHPGV
HHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCHHHHHHHCCCCEEEEEECCCCCCCCC
TATFCEALAAVGVNIELISTSEIRISVLCRDTELDKAVVALHEAFGLGGDEEATVYAGTG
HHHHHHHHHHCCCEEEEEECCCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCEEEEECCC
R
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972; 7910936