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The map label for this gene is gshA

Identifier: 148663568

GI number: 148663568

Start: 4155334

End: 4156632

Strand: Reverse

Name: gshA

Synonym: MRA_3740

Alternate gene names: NA

Gene position: 4156632-4155334 (Counterclockwise)

Preceding gene: 148663569

Following gene: 148663567

Centisome position: 94.04

GC content: 68.28

Gene sequence:

>1299_bases
ATGACGCTTGCCGCCATGACCGCCGCGGCCTCGCAGCTGGATAACGCTGCCCCGGACGACGTCGAGATCACCGATTCATC
GGCCGCCGCGGAATACATCGCGGACGGCTGCCTCGTCGATGGTCCGTTGGGACGCGTCGGTCTGGAGATGGAGGCGCACT
GCTTCGACCCGGCCGATCCGTTTCGCCGGCCCAGCTGGGAAGAGATCACCGAGGTTCTCGAATGGCTCAGTCCGCTGCCG
GGCGGCAGCGTGGTCAGCGTGGAGCCCGGTGGTGCCGTCGAACTGTCCGGACCGCCCGCCGACGGCGTGCTGGCAGCCAT
CGGTGCCATGACGCGCGATCAGGCGGTGCTACGGTCGGCGCTTGCCAATGCCGGGCTGGGTTTGGTCTTCCTGGGTGCAG
ACCCGCTGCGGTCGCCGGTGCGGGTCAATCCGGGCGCACGCTATCGGGCCATGGAGCAGTTTTTCGCCGCCAGCCATAGC
GGAGTGCCGGGTGCGGCGATGATGACCTCGACAGCCGCGATCCAGGTCAATCTGGATGCCGGACCGCAGGAAGGATGGGC
TGAGCGAGTCCGCCTGGCGCACGCCTTGGGGCCGACGATGATCGCGATTGCCGCCAACTCCCCGATGCTGGGCGGCCGGT
TTTCCGGCTGGCAGTCCACCCGGCAGCGGGTGTGGGGCCAGATGGACTCCGCGCGCTGCGGCCCCATTCTTGGTGCCAGT
GGCGACCACCCCGGCATCGACTGGGCTAAGTATGCCTTGAAGGCGCCGGTGATGATGGTGCGAAGCCCGGACACTCAAGA
CACCCGTGCGGTGACGGACTACGTGCCCTTCACCGACTGGGTTGACGGTCGGGTGCTGCTGGACGGTCGCCGCGCTACCG
TCGCCGACCTGGTTTACCACCTGACAACGTTGTTCCCGCCGGTGCGTCCTCGGCAATGGCTGGAAATTCGCTACCTCGAC
AGCGTGCCCGACGAGGTGTGGCCCGCGGTGGTGTTCACCTTGGTGACGCTGCTCGACGACCCGGTGGCGGCAGATTTGGC
GGTCGACGCGGTCGAACCGGTGGCCACGGCCTGGGACACCGCGGCCCGGATCGGCCTGGCCGACCGGCGGCTCTACCTGG
CAGCCAACAGATGCCTGGCCATCGCCGCGCGGCGGGTACCGACTGAACTCATCGGCGCGATGCAGCGGTTGGTGGACCAC
GTCGACAGGGGTGTTTGCCCGGCAGACGATTTCTCCGACCGGGTGATCGCGGGCGGCATCGCGTCAGCCGTCACCGGGAT
GATGCACGGGGCGTCGTGA

Upstream 100 bases:

>100_bases
CGGCAGGATAAACCGGTGGCGGCTATCGTTCTGGTAGCTGCGCAACCAACGGTCCGCAGGGCATGTTTTAGGCATGCGCC
GGGCCCGGAGGGATCGTGAT

Downstream 100 bases:

>100_bases
CTTCACCCGAGCAGCTGGCTTGTCATCTGGCGCGGGCGCGGGCGCGGACGTTGCGGCTGGTCGACTTCGACGATGCCGAG
CTTTGCTGCCAGTATGACCC

Product: putative glutamate--cysteine ligase

Products: NA

Alternate protein names: Glutamate/Cysteine Ligase; Gamma-Glutamylcysteine Synthetase; Glutamate/Cysteine Ligase Family Protein; Glutamate-Cysteine Ligase GshA; Glutamylcysteine Synthetase; Glutamate-Cysteine Ligase Gsha; Glutamate-Cysteine Ligase GSHA; Glutamate-Cysteine Ligase Protein; Glutathione Synthetase; Glutamate-Cysteine Ligase Protein; Glutamate-Cysteine Ligase Family 2 Protein; Glutamate-Cysteine Ligase Family Protein; Glutamate Cystein Ligase Protein

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MTLAAMTAAASQLDNAAPDDVEITDSSAAAEYIADGCLVDGPLGRVGLEMEAHCFDPADPFRRPSWEEITEVLEWLSPLP
GGSVVSVEPGGAVELSGPPADGVLAAIGAMTRDQAVLRSALANAGLGLVFLGADPLRSPVRVNPGARYRAMEQFFAASHS
GVPGAAMMTSTAAIQVNLDAGPQEGWAERVRLAHALGPTMIAIAANSPMLGGRFSGWQSTRQRVWGQMDSARCGPILGAS
GDHPGIDWAKYALKAPVMMVRSPDTQDTRAVTDYVPFTDWVDGRVLLDGRRATVADLVYHLTTLFPPVRPRQWLEIRYLD
SVPDEVWPAVVFTLVTLLDDPVAADLAVDAVEPVATAWDTAARIGLADRRLYLAANRCLAIAARRVPTELIGAMQRLVDH
VDRGVCPADDFSDRVIAGGIASAVTGMMHGAS

Sequences:

>Translated_432_residues
MTLAAMTAAASQLDNAAPDDVEITDSSAAAEYIADGCLVDGPLGRVGLEMEAHCFDPADPFRRPSWEEITEVLEWLSPLP
GGSVVSVEPGGAVELSGPPADGVLAAIGAMTRDQAVLRSALANAGLGLVFLGADPLRSPVRVNPGARYRAMEQFFAASHS
GVPGAAMMTSTAAIQVNLDAGPQEGWAERVRLAHALGPTMIAIAANSPMLGGRFSGWQSTRQRVWGQMDSARCGPILGAS
GDHPGIDWAKYALKAPVMMVRSPDTQDTRAVTDYVPFTDWVDGRVLLDGRRATVADLVYHLTTLFPPVRPRQWLEIRYLD
SVPDEVWPAVVFTLVTLLDDPVAADLAVDAVEPVATAWDTAARIGLADRRLYLAANRCLAIAARRVPTELIGAMQRLVDH
VDRGVCPADDFSDRVIAGGIASAVTGMMHGAS
>Mature_431_residues
TLAAMTAAASQLDNAAPDDVEITDSSAAAEYIADGCLVDGPLGRVGLEMEAHCFDPADPFRRPSWEEITEVLEWLSPLPG
GSVVSVEPGGAVELSGPPADGVLAAIGAMTRDQAVLRSALANAGLGLVFLGADPLRSPVRVNPGARYRAMEQFFAASHSG
VPGAAMMTSTAAIQVNLDAGPQEGWAERVRLAHALGPTMIAIAANSPMLGGRFSGWQSTRQRVWGQMDSARCGPILGASG
DHPGIDWAKYALKAPVMMVRSPDTQDTRAVTDYVPFTDWVDGRVLLDGRRATVADLVYHLTTLFPPVRPRQWLEIRYLDS
VPDEVWPAVVFTLVTLLDDPVAADLAVDAVEPVATAWDTAARIGLADRRLYLAANRCLAIAARRVPTELIGAMQRLVDHV
DRGVCPADDFSDRVIAGGIASAVTGMMHGAS

Specific function: Unknown

COG id: COG3572

COG function: function code H; Gamma-glutamylcysteine synthetase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 45796; Mature: 45664

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLAAMTAAASQLDNAAPDDVEITDSSAAAEYIADGCLVDGPLGRVGLEMEAHCFDPADP
CCHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHCCCEECCCCCCCCCCEEECCCCCCCC
FRRPSWEEITEVLEWLSPLPGGSVVSVEPGGAVELSGPPADGVLAAIGAMTRDQAVLRSA
CCCCCHHHHHHHHHHHCCCCCCCEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHH
LANAGLGLVFLGADPLRSPVRVNPGARYRAMEQFFAASHSGVPGAAMMTSTAAIQVNLDA
HHHCCCCEEEECCCCCCCCEEECCCHHHHHHHHHHHHCCCCCCCHHHEECEEEEEEEECC
GPQEGWAERVRLAHALGPTMIAIAANSPMLGGRFSGWQSTRQRVWGQMDSARCGPILGAS
CCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
GDHPGIDWAKYALKAPVMMVRSPDTQDTRAVTDYVPFTDWVDGRVLLDGRRATVADLVYH
CCCCCCHHHHHHHHCCEEEEECCCCCCCHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHH
LTTLFPPVRPRQWLEIRYLDSVPDEVWPAVVFTLVTLLDDPVAADLAVDAVEPVATAWDT
HHHHCCCCCCCCEEEEEEHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AARIGLADRRLYLAANRCLAIAARRVPTELIGAMQRLVDHVDRGVCPADDFSDRVIAGGI
HHHHCCCCCEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
ASAVTGMMHGAS
HHHHHHHHCCCC
>Mature Secondary Structure 
TLAAMTAAASQLDNAAPDDVEITDSSAAAEYIADGCLVDGPLGRVGLEMEAHCFDPADP
CHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHHCCCEECCCCCCCCCCEEECCCCCCCC
FRRPSWEEITEVLEWLSPLPGGSVVSVEPGGAVELSGPPADGVLAAIGAMTRDQAVLRSA
CCCCCHHHHHHHHHHHCCCCCCCEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHH
LANAGLGLVFLGADPLRSPVRVNPGARYRAMEQFFAASHSGVPGAAMMTSTAAIQVNLDA
HHHCCCCEEEECCCCCCCCEEECCCHHHHHHHHHHHHCCCCCCCHHHEECEEEEEEEECC
GPQEGWAERVRLAHALGPTMIAIAANSPMLGGRFSGWQSTRQRVWGQMDSARCGPILGAS
CCCHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
GDHPGIDWAKYALKAPVMMVRSPDTQDTRAVTDYVPFTDWVDGRVLLDGRRATVADLVYH
CCCCCCHHHHHHHHCCEEEEECCCCCCCHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHH
LTTLFPPVRPRQWLEIRYLDSVPDEVWPAVVFTLVTLLDDPVAADLAVDAVEPVATAWDT
HHHHCCCCCCCCEEEEEEHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AARIGLADRRLYLAANRCLAIAARRVPTELIGAMQRLVDHVDRGVCPADDFSDRVIAGGI
HHHHCCCCCEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
ASAVTGMMHGAS
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA