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The map label for this gene is cysM

Identifier: 148661125

GI number: 148661125

Start: 1504704

End: 1505675

Strand: Direct

Name: cysM

Synonym: MRA_1344

Alternate gene names: 148661125

Gene position: 1504704-1505675 (Clockwise)

Preceding gene: 148661124

Following gene: 148661126

Centisome position: 34.04

GC content: 66.67

Gene sequence:

>972_bases
ATGACACGATACGACTCGCTGTTGCAGGCCTTGGGCAACACGCCGCTGGTTGGCCTGCAGCGATTGTCGCCACGCTGGGA
TGACGGGCGAGACGGACCGCACGTGCGGCTGTGGGCCAAGCTCGAGGACCGCAATCCGACCGGGTCGATCAAGGACCGCC
CGGCTGTGCGGATGATCGAGCAGGCCGAGGCCGACGGGTTGTTGCGGCCGGGCGCCACCATCCTGGAGCCCACCAGCGGA
AACACCGGCATTTCGCTGGCGATGGCGGCCCGGTTGAAGGGGTACCGATTGATCTGCGTGATGCCGGAGAACACATCGGT
TGAACGGCGGCAGCTGCTCGAGCTCTACGGCGCGCAGATTATCTTCTCGGCGGCCGAAGGCGGGTCCAACACTGCGGTGG
CCACCGCCAAAGAGCTGGCCGCGACCAACCCGTCATGGGTGATGCTGTACCAGTACGGCAATCCCGCCAACACCGACTCG
CACTACTGCGGCACCGGCCCCGAGCTGCTGGCCGACCTGCCCGAAATCACGCACTTCGTCGCCGGCCTAGGCACCACGGG
CACGCTGATGGGCACTGGCCGTTTCCTGCGCGAGCACGTTGCCAACGTCAAGATCGTGGCGGCCGAACCCCGCTACGGTG
AGGGGGTATACGCCCTGCGCAACATGGACGAAGGCTTTGTGCCCGAGCTGTATGACCCGGAAATACTGACCGCGCGATAT
TCTGTCGGCGCGGTGGACGCAGTGCGCCGCACCCGCGAGTTGGTGCACACCGAAGGCATCTTTGCGGGCATCTCAACCGG
CGCGGTGCTACACGCCGCACTCGGAGTCGGGGCCGGCGCCCTGGCGGCCGGCGAGCGGGCCGACATTGCGTTGGTGGTCG
CCGACGCCGGGTGGAAGTATCTGTCCACCGGCGCCTACGCCGGTAGCCTGGATGACGCCGAGACCGCTCTGGAAGGGCAA
CTATGGGCATGA

Upstream 100 bases:

>100_bases
CGACGAGGACGTGCGGTTCTCCGGCGGCTTGGCCACCGCGATCGCTGACGGTGACTCGGTCACCATCCTCCCCGCCGTGG
CCGGTGGGTGAGCGGAGCAC

Downstream 100 bases:

>100_bases
CCCCGCGCCGGAAGCGACGGGGAGGAGCGGTGCAGATAACACGGCCCACAGGCCGTCCGCGAACACCGACAACGCAGACG
ACGAAGCGCCCGCGCTGGGT

Product: cysteine synthase

Products: NA

Alternate protein names: OPS sulfhydrylase; CysO-thiocarboxylate-dependent cysteine synthase; Cysteine synthase B; CSase B; O-phosphoserine-specific cysteine synthase

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MTRYDSLLQALGNTPLVGLQRLSPRWDDGRDGPHVRLWAKLEDRNPTGSIKDRPAVRMIEQAEADGLLRPGATILEPTSG
NTGISLAMAARLKGYRLICVMPENTSVERRQLLELYGAQIIFSAAEGGSNTAVATAKELAATNPSWVMLYQYGNPANTDS
HYCGTGPELLADLPEITHFVAGLGTTGTLMGTGRFLREHVANVKIVAAEPRYGEGVYALRNMDEGFVPELYDPEILTARY
SVGAVDAVRRTRELVHTEGIFAGISTGAVLHAALGVGAGALAAGERADIALVVADAGWKYLSTGAYAGSLDDAETALEGQ
LWA

Sequences:

>Translated_323_residues
MTRYDSLLQALGNTPLVGLQRLSPRWDDGRDGPHVRLWAKLEDRNPTGSIKDRPAVRMIEQAEADGLLRPGATILEPTSG
NTGISLAMAARLKGYRLICVMPENTSVERRQLLELYGAQIIFSAAEGGSNTAVATAKELAATNPSWVMLYQYGNPANTDS
HYCGTGPELLADLPEITHFVAGLGTTGTLMGTGRFLREHVANVKIVAAEPRYGEGVYALRNMDEGFVPELYDPEILTARY
SVGAVDAVRRTRELVHTEGIFAGISTGAVLHAALGVGAGALAAGERADIALVVADAGWKYLSTGAYAGSLDDAETALEGQ
LWA
>Mature_322_residues
TRYDSLLQALGNTPLVGLQRLSPRWDDGRDGPHVRLWAKLEDRNPTGSIKDRPAVRMIEQAEADGLLRPGATILEPTSGN
TGISLAMAARLKGYRLICVMPENTSVERRQLLELYGAQIIFSAAEGGSNTAVATAKELAATNPSWVMLYQYGNPANTDSH
YCGTGPELLADLPEITHFVAGLGTTGTLMGTGRFLREHVANVKIVAAEPRYGEGVYALRNMDEGFVPELYDPEILTARYS
VGAVDAVRRTRELVHTEGIFAGISTGAVLHAALGVGAGALAAGERADIALVVADAGWKYLSTGAYAGSLDDAETALEGQL
WA

Specific function: Catalyzes the formation of a covalent CysO-cysteine adduct via a sulfur transfer, using the thiocarboxylated sulfur carrier protein CysO-COSH as sulfur donor and O-phospho-L-serine (OPS) as sulfur acceptor. Can also use sodium sulfide as sulfur donor in v

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family

Homologues:

Organism=Homo sapiens, GI295821202, Length=312, Percent_Identity=32.3717948717949, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI295821200, Length=312, Percent_Identity=32.3717948717949, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI4557415, Length=312, Percent_Identity=32.3717948717949, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI11345492, Length=213, Percent_Identity=27.2300469483568, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI2367138, Length=302, Percent_Identity=41.0596026490066, Blast_Score=215, Evalue=3e-57,
Organism=Escherichia coli, GI1788754, Length=321, Percent_Identity=34.8909657320872, Blast_Score=169, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI17535051, Length=314, Percent_Identity=36.624203821656, Blast_Score=184, Evalue=5e-47,
Organism=Caenorhabditis elegans, GI17562970, Length=311, Percent_Identity=35.048231511254, Blast_Score=168, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI32566674, Length=273, Percent_Identity=37.3626373626374, Blast_Score=165, Evalue=4e-41,
Organism=Caenorhabditis elegans, GI115535073, Length=305, Percent_Identity=35.4098360655738, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17561720, Length=311, Percent_Identity=33.7620578778135, Blast_Score=143, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17534315, Length=317, Percent_Identity=33.4384858044164, Blast_Score=130, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI25147552, Length=316, Percent_Identity=31.9620253164557, Blast_Score=127, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI32566672, Length=220, Percent_Identity=31.3636363636364, Blast_Score=101, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6321594, Length=340, Percent_Identity=32.9411764705882, Blast_Score=169, Evalue=6e-43,
Organism=Saccharomyces cerevisiae, GI6321449, Length=351, Percent_Identity=30.7692307692308, Blast_Score=100, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24643623, Length=303, Percent_Identity=36.6336633663366, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI20129101, Length=303, Percent_Identity=36.6336633663366, Blast_Score=167, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSM_MYCBO (P63874)

Other databases:

- EMBL:   BX248338
- RefSeq:   NP_855025.1
- ProteinModelPortal:   P63874
- SMR:   P63874
- EnsemblBacteria:   EBMYCT00000014909
- GeneID:   1090662
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb1371
- GeneTree:   EBGT00050000015764
- HOGENOM:   HBG748215
- OMA:   YGLRNID
- ProtClustDB:   CLSK871928
- BioCyc:   MBOV233413:MB1371-MONOMER
- BRENDA:   2.5.1.47
- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR001926
- TIGRFAMs:   TIGR01136

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =2.5.1.65

Molecular weight: Translated: 34438; Mature: 34307

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: BINDING 81-81 BINDING 220-220 BINDING 265-265

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRYDSLLQALGNTPLVGLQRLSPRWDDGRDGPHVRLWAKLEDRNPTGSIKDRPAVRMIE
CCHHHHHHHHHCCCCEEEHHHCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHH
QAEADGLLRPGATILEPTSGNTGISLAMAARLKGYRLICVMPENTSVERRQLLELYGAQI
HHCCCCCCCCCCEEEECCCCCCCEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHCCEE
IFSAAEGGSNTAVATAKELAATNPSWVMLYQYGNPANTDSHYCGTGPELLADLPEITHFV
EEEECCCCCCCEEHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHH
AGLGTTGTLMGTGRFLREHVANVKIVAAEPRYGEGVYALRNMDEGFVPELYDPEILTARY
HCCCCCCEEHHHHHHHHHHHCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCEEEEEC
SVGAVDAVRRTRELVHTEGIFAGISTGAVLHAALGVGAGALAAGERADIALVVADAGWKY
CCCHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHCCCCHHCCCCCCCEEEEEECCCCEE
LSTGAYAGSLDDAETALEGQLWA
EECCCCCCCCCCHHHHHCCCCCC
>Mature Secondary Structure 
TRYDSLLQALGNTPLVGLQRLSPRWDDGRDGPHVRLWAKLEDRNPTGSIKDRPAVRMIE
CHHHHHHHHHCCCCEEEHHHCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHH
QAEADGLLRPGATILEPTSGNTGISLAMAARLKGYRLICVMPENTSVERRQLLELYGAQI
HHCCCCCCCCCCEEEECCCCCCCEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHCCEE
IFSAAEGGSNTAVATAKELAATNPSWVMLYQYGNPANTDSHYCGTGPELLADLPEITHFV
EEEECCCCCCCEEHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHH
AGLGTTGTLMGTGRFLREHVANVKIVAAEPRYGEGVYALRNMDEGFVPELYDPEILTARY
HCCCCCCEEHHHHHHHHHHHCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCEEEEEC
SVGAVDAVRRTRELVHTEGIFAGISTGAVLHAALGVGAGALAAGERADIALVVADAGWKY
CCCHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHCCCCHHCCCCCCCEEEEEECCCCEE
LSTGAYAGSLDDAETALEGQLWA
EECCCCCCCCCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972