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The map label for this gene is ptrBa [C]

Identifier: 148660558

GI number: 148660558

Start: 875539

End: 876249

Strand: Direct

Name: ptrBa [C]

Synonym: MRA_0790

Alternate gene names: 148660558

Gene position: 875539-876249 (Clockwise)

Preceding gene: 148660557

Following gene: 148660559

Centisome position: 19.81

GC content: 59.49

Gene sequence:

>711_bases
ATGATGCACCGAACCGCACTACCCTCACCGCCCGTGGCCAAGCGGGTGCAGACCCGCCGGGAGCACCACGGCGACGTCTT
TGTCGACCCATATGAATGGTTGCGCGACAAGGACAGCCCTGAAGTAATCGCCTACCTCGAAGCTGAAAACGACTACACCG
AACGGACCACCGCGCACCTTGAGCCATTGCGGCAAAAGATCTTCCACGAAATCAAAGCGCGTACCAAGGAAACCGACTTA
TCGGTGCCGACGCGACGTGGCAACTGGTGGTACTACGCGCGGACCTTTGAGGGAAAGCAGTATGGCGTACACTGTCGTTG
CCCGGTAACCGATCCCGACGACTGGAACCCACCAGAGTTCGACGAGCGCACCGAAATACCCGGTGAACAGCTTCTGCTCG
ACGAGAACGTGGAAGCTGACGGCCACGACTTCTTCGCACTGGGCGCGGCCAGCGTCAGCCTGGACGATAACCTCTTAGCG
TATTCCGTTGATGTCGTAGGTGACGAACGATATACCTTGCGGTTCAAGGATTTACGCACCGGAGAACAGTACCCGGACGA
GATCGCCGGGATCGGAGCGGGAGTCACCTGGGCAGCTGACAACCACTGTCTACTACACCACCGTGGACGCGGCCTGGCGT
CCGGACACAGTGTGGCGATACCGACTAGGGTCCGGCGAATCGTCGGAGCGGGTTTACCACGAAGCCGATGA

Upstream 100 bases:

>100_bases
CGCTCCCCGAGCATATCGTCGAGGCCACGCGTGCCCGTTATATTAATGCATACGAACGGATTTCCGAACTAAAATTCGAC
GACTGGATCGGCCCTGGCGC

Downstream 100 bases:

>100_bases
TCGGTTCTGGCTCGCGGTGGGGCGTACTCGCAGCAACGCCTATCTGCTGATTGCGGCGGGGTCGTCCATCACTTCGGAGG
TCCGTTACGCGCACGCGGCA

Product: putative protease II PtrBa

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MMHRTALPSPPVAKRVQTRREHHGDVFVDPYEWLRDKDSPEVIAYLEAENDYTERTTAHLEPLRQKIFHEIKARTKETDL
SVPTRRGNWWYYARTFEGKQYGVHCRCPVTDPDDWNPPEFDERTEIPGEQLLLDENVEADGHDFFALGAASVSLDDNLLA
YSVDVVGDERYTLRFKDLRTGEQYPDEIAGIGAGVTWAADNHCLLHHRGRGLASGHSVAIPTRVRRIVGAGLPRSR

Sequences:

>Translated_236_residues
MMHRTALPSPPVAKRVQTRREHHGDVFVDPYEWLRDKDSPEVIAYLEAENDYTERTTAHLEPLRQKIFHEIKARTKETDL
SVPTRRGNWWYYARTFEGKQYGVHCRCPVTDPDDWNPPEFDERTEIPGEQLLLDENVEADGHDFFALGAASVSLDDNLLA
YSVDVVGDERYTLRFKDLRTGEQYPDEIAGIGAGVTWAADNHCLLHHRGRGLASGHSVAIPTRVRRIVGAGLPRSR
>Mature_236_residues
MMHRTALPSPPVAKRVQTRREHHGDVFVDPYEWLRDKDSPEVIAYLEAENDYTERTTAHLEPLRQKIFHEIKARTKETDL
SVPTRRGNWWYYARTFEGKQYGVHCRCPVTDPDDWNPPEFDERTEIPGEQLLLDENVEADGHDFFALGAASVSLDDNLLA
YSVDVVGDERYTLRFKDLRTGEQYPDEIAGIGAGVTWAADNHCLLHHRGRGLASGHSVAIPTRVRRIVGAGLPRSR

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1770

COG function: function code E; Protease II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9A family [H]

Homologues:

Organism=Escherichia coli, GI1788150, Length=200, Percent_Identity=28.5, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 26916; Mature: 26916

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMHRTALPSPPVAKRVQTRREHHGDVFVDPYEWLRDKDSPEVIAYLEAENDYTERTTAHL
CCCCCCCCCCHHHHHHHHHHHHCCCEEECHHHHHCCCCCCCEEEEEECCCCCCHHHHHHH
EPLRQKIFHEIKARTKETDLSVPTRRGNWWYYARTFEGKQYGVHCRCPVTDPDDWNPPEF
HHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCEECEEEECCCCCCCCCCCCCC
DERTEIPGEQLLLDENVEADGHDFFALGAASVSLDDNLLAYSVDVVGDERYTLRFKDLRT
CCCCCCCCHHEEEECCCCCCCCEEEEEEEEEEECCCCEEEEEEEEECCCEEEEEEEECCC
GEQYPDEIAGIGAGVTWAADNHCLLHHRGRGLASGHSVAIPTRVRRIVGAGLPRSR
CCCCCHHHHHCCCCEEEECCCEEEEEECCCCCCCCCCEECHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MMHRTALPSPPVAKRVQTRREHHGDVFVDPYEWLRDKDSPEVIAYLEAENDYTERTTAHL
CCCCCCCCCCHHHHHHHHHHHHCCCEEECHHHHHCCCCCCCEEEEEECCCCCCHHHHHHH
EPLRQKIFHEIKARTKETDLSVPTRRGNWWYYARTFEGKQYGVHCRCPVTDPDDWNPPEF
HHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCEECEEEECCCCCCCCCCCCCC
DERTEIPGEQLLLDENVEADGHDFFALGAASVSLDDNLLAYSVDVVGDERYTLRFKDLRT
CCCCCCCCHHEEEECCCCCCCCEEEEEEEEEEECCCCEEEEEEEEECCCEEEEEEEECCC
GEQYPDEIAGIGAGVTWAADNHCLLHHRGRGLASGHSVAIPTRVRRIVGAGLPRSR
CCCCCHHHHHCCCCEEEECCCEEEEEECCCCCCCCCCEECHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.23 {tosyl-Arg} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 0.92 {benzoyl-Lys} 0.6 {N-benzoyl-Arg} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 80 {acetyl-tyrosine} 0.47 {tosyl-Lys-methyl} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]