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The map label for this gene is hemH

Identifier: 148660557

GI number: 148660557

Start: 874649

End: 875542

Strand: Direct

Name: hemH

Synonym: MRA_0789

Alternate gene names: 148660557

Gene position: 874649-875542 (Clockwise)

Preceding gene: 148660555

Following gene: 148660558

Centisome position: 19.79

GC content: 63.76

Gene sequence:

>894_bases
ATGCGCCCCGCATTGTCCGACTACCAGCATGTGGCCAGCGGTAAGGTCCGCGAGATCTACCGTGTCGATGACGAGCACCT
GCTGCTGGTTGCCAGCGACCGGATCTCGGCGTACGACTACGTCCTGGACAGCACCATCCCGGACAAGGGCCGCGTCCTGA
CCGCCATGAGCGCATTCTTCTTCGGGCTCGTCGATGCCCCTAACCATCTGGCCGGGCCGCCGGACGACCCGCGTATCCCC
GACGAGGTGCTGGGCCGCGCGCTGGTGGTGCGTCGGCTGGAGATGCTGCCGGTGGAATGTGTGGCCCGTGGCTACCTGAC
CGGTTCGGGGTTACTGGATTACCAGGCAACCGGGAAGGTATGCGGTATCGCGCTGCCGCCGGGCCTGGTCGAGGCCAGTC
GGTTCGCCACACCGCTGTTCACCCCGGCGACTAAAGCCGCGTTGGGGGACCACGACGAGAACATCTCGTTTGACCGGGTG
GTGGAGATGGTAGGCGCGTTGCGTGCCAACCAGCTGCGTGATCGTACTCTGCAGACGTATGTGCAGGCCGCCGATCACGC
TCTCACCCGCGGAATCATTATCGCCGACACCAAGTTTGAATTTGGCATCGACCGCCACGGCAACCTGCTGCTGGCCGACG
AAATCTTCACACCGGACTCGTCGCGGTACTGGCCTGCCGACGACTACCGGGCCGGCGTGGTCCAGACCAGCTTCGACAAA
CAGTTTGTCCGCAGCTGGCTCACCGGCTCCGAGTCCGGCTGGGATAGAGGCAGCGATCGGCCGCCGCCTCCGCTCCCCGA
GCATATCGTCGAGGCCACGCGTGCCCGTTATATTAATGCATACGAACGGATTTCCGAACTAAAATTCGACGACTGGATCG
GCCCTGGCGCATGA

Upstream 100 bases:

>100_bases
GCCAGCAGTCGGCCGGGCGTGGTGGCATACGGCAAGAAGCGCGATCTCATACCAGAGATACCAGCACAGGGCGCCGTCGT
GCGGCGGATAGGCTGGCGCG

Downstream 100 bases:

>100_bases
TGCACCGAACCGCACTACCCTCACCGCCCGTGGCCAAGCGGGTGCAGACCCGCCGGGAGCACCACGGCGACGTCTTTGTC
GACCCATATGAATGGTTGCG

Product: phosphoribosylaminoimidazole-succinocarboxamide synthase

Products: NA

Alternate protein names: SAICAR synthetase

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MRPALSDYQHVASGKVREIYRVDDEHLLLVASDRISAYDYVLDSTIPDKGRVLTAMSAFFFGLVDAPNHLAGPPDDPRIP
DEVLGRALVVRRLEMLPVECVARGYLTGSGLLDYQATGKVCGIALPPGLVEASRFATPLFTPATKAALGDHDENISFDRV
VEMVGALRANQLRDRTLQTYVQAADHALTRGIIIADTKFEFGIDRHGNLLLADEIFTPDSSRYWPADDYRAGVVQTSFDK
QFVRSWLTGSESGWDRGSDRPPPPLPEHIVEATRARYINAYERISELKFDDWIGPGA

Sequences:

>Translated_297_residues
MRPALSDYQHVASGKVREIYRVDDEHLLLVASDRISAYDYVLDSTIPDKGRVLTAMSAFFFGLVDAPNHLAGPPDDPRIP
DEVLGRALVVRRLEMLPVECVARGYLTGSGLLDYQATGKVCGIALPPGLVEASRFATPLFTPATKAALGDHDENISFDRV
VEMVGALRANQLRDRTLQTYVQAADHALTRGIIIADTKFEFGIDRHGNLLLADEIFTPDSSRYWPADDYRAGVVQTSFDK
QFVRSWLTGSESGWDRGSDRPPPPLPEHIVEATRARYINAYERISELKFDDWIGPGA
>Mature_297_residues
MRPALSDYQHVASGKVREIYRVDDEHLLLVASDRISAYDYVLDSTIPDKGRVLTAMSAFFFGLVDAPNHLAGPPDDPRIP
DEVLGRALVVRRLEMLPVECVARGYLTGSGLLDYQATGKVCGIALPPGLVEASRFATPLFTPATKAALGDHDENISFDRV
VEMVGALRANQLRDRTLQTYVQAADHALTRGIIIADTKFEFGIDRHGNLLLADEIFTPDSSRYWPADDYRAGVVQTSFDK
QFVRSWLTGSESGWDRGSDRPPPPLPEHIVEATRARYINAYERISELKFDDWIGPGA

Specific function: De novo purine biosynthesis; seventh step. [C]

COG id: COG0152

COG function: function code F; Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SAICAR synthetase family

Homologues:

Organism=Saccharomyces cerevisiae, GI6319326, Length=295, Percent_Identity=42.7118644067797, Blast_Score=241, Evalue=7e-65,
Organism=Drosophila melanogaster, GI24583917, Length=235, Percent_Identity=27.6595744680851, Blast_Score=71, Evalue=8e-13,

Paralogues:

None

Copy number: 1680 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 13,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PUR7_MYCBO (P0A5T5)

Other databases:

- EMBL:   BX248336
- RefSeq:   NP_854461.1
- ProteinModelPortal:   P0A5T5
- SMR:   P0A5T5
- EnsemblBacteria:   EBMYCT00000014897
- GeneID:   1092526
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb0803
- GeneTree:   EBGT00050000017539
- HOGENOM:   HBG555040
- OMA:   LTLMDEV
- ProtClustDB:   PRK13961
- BioCyc:   MBOV233413:MB0803-MONOMER
- BRENDA:   6.3.2.6
- HAMAP:   MF_00137
- InterPro:   IPR013816
- InterPro:   IPR001636
- InterPro:   IPR018236
- Gene3D:   G3DSA:3.30.470.20
- PANTHER:   PTHR11609
- TIGRFAMs:   TIGR00081

Pfam domain/function: PF01259 SAICAR_synt

EC number: =6.3.2.6

Molecular weight: Translated: 32930; Mature: 32930

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS01057 SAICAR_SYNTHETASE_1; PS01058 SAICAR_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPALSDYQHVASGKVREIYRVDDEHLLLVASDRISAYDYVLDSTIPDKGRVLTAMSAFF
CCCCHHHHHHHHCCCHHHHEECCCCEEEEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHH
FGLVDAPNHLAGPPDDPRIPDEVLGRALVVRRLEMLPVECVARGYLTGSGLLDYQATGKV
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEECCCCE
CGIALPPGLVEASRFATPLFTPATKAALGDHDENISFDRVVEMVGALRANQLRDRTLQTY
EEEECCCCHHHHHHHCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VQAADHALTRGIIIADTKFEFGIDRHGNLLLADEIFTPDSSRYWPADDYRAGVVQTSFDK
HHHHHHHHHCCEEEEECCEEECCCCCCCEEEEECEECCCCCCCCCCCCCCCCCEECHHHH
QFVRSWLTGSESGWDRGSDRPPPPLPEHIVEATRARYINAYERISELKFDDWIGPGA
HHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCC
>Mature Secondary Structure
MRPALSDYQHVASGKVREIYRVDDEHLLLVASDRISAYDYVLDSTIPDKGRVLTAMSAFF
CCCCHHHHHHHHCCCHHHHEECCCCEEEEEECCCCCHHHHHHHCCCCCCCHHHHHHHHHH
FGLVDAPNHLAGPPDDPRIPDEVLGRALVVRRLEMLPVECVARGYLTGSGLLDYQATGKV
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEECCCCE
CGIALPPGLVEASRFATPLFTPATKAALGDHDENISFDRVVEMVGALRANQLRDRTLQTY
EEEECCCCHHHHHHHCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
VQAADHALTRGIIIADTKFEFGIDRHGNLLLADEIFTPDSSRYWPADDYRAGVVQTSFDK
HHHHHHHHHCCEEEEECCEEECCCCCCCEEEEECEECCCCCCCCCCCCCCCCCEECHHHH
QFVRSWLTGSESGWDRGSDRPPPPLPEHIVEATRARYINAYERISELKFDDWIGPGA
HHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972