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The map label for this gene is garR [C]

Identifier: 148660547

GI number: 148660547

Start: 864604

End: 865449

Strand: Direct

Name: garR [C]

Synonym: MRA_0779

Alternate gene names: 148660547

Gene position: 864604-865449 (Clockwise)

Preceding gene: 148660546

Following gene: 148660548

Centisome position: 19.56

GC content: 66.19

Gene sequence:

>846_bases
TTGGGTAATCAAGGCGCGCCGATGGCTAAGCGTCTGCTCGATTGGCCTGGCGGACTGACCGTTTTCGATGTGCGGGTCGA
GGCCATGGCACCGTTCGTCGAGGGCGGCGCCACCGCAGCGGCAAGCGTCTCCGACGTCGCCGAAGCCGACATCATCAGCA
TCACCGTGTTCGACGACGCGCAGGTGAGTTCGGTGATCACCGCCGACAACGGACTGGCGACGCACGCCAAGCCCGGCACT
ATTGTCGCGATTCACTCCACCATCGCCGACACGACAGCAGTCGATCTGGCCGAAAAGCTCAAGCCGCAGGGGATCCACAT
CGTGGATGCACCGGTCAGCGGCGGCGCGGCGGCGGCCGCCAAGGGTGAGTTGGCCGTGATGGTCGGCGCTGACGACGAGG
CGTTCCAGCGGATTAAAGAGCCATTTTCGAGGTGGGCTTCGCTGTTGATTCATGCCGGGGAACCGGGCGCTGGCACCCGG
ATGAAACTGGCGCGCAACATGTTGACTTTCGTCTCTTATGCCGCCGCCGCCGAGGCGCAGCGGCTGGCCGAAGCCTGTGG
CTTAGACCTCGTGGCGCTCGGGAAGGTGGTGCGGCACAGCGACTCATTCACCGGCGGCGCGGGAGCGATCATGTTCCGCA
ACACCACTGCGCCGATGGAGCCGGCTGACCCGCTGCGGCCGTTGTTGGAGCACACCCGCGGCCTGGGTGAGAAAGACCTG
AGTCTGGCGTTGGCCCTGGGCGAGGTGGTATCGGTCGACCTGCCGCTGGCCCAGCTGGCGCTGCAACGGCTGGCCGCCGG
CCTCGGGGTACCGCACCCGGACACCGAGCCAGCAAAGGAGACATGA

Upstream 100 bases:

>100_bases
GGGCACGCCCCCACAAGTGGGAGGTACCCCCATCCGCTGGCGGGGGAGAGCGGCGCTCATGACCGCTCACCCGGAGACAC
CACGCCTGGGATATATCGGC

Downstream 100 bases:

>100_bases
TGGACGAGCTGCGCCGCACCGGCCTGGACAAAATGAACGAGGTTTACGCCTGGGACATGCCCGACATGCCAGGTGAGTTT
TTTGCCCTGACCGTCGATCA

Product: putative dehydrogenase/reductase

Products: 2-methyl-3-oxopropanoate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MGNQGAPMAKRLLDWPGGLTVFDVRVEAMAPFVEGGATAAASVSDVAEADIISITVFDDAQVSSVITADNGLATHAKPGT
IVAIHSTIADTTAVDLAEKLKPQGIHIVDAPVSGGAAAAAKGELAVMVGADDEAFQRIKEPFSRWASLLIHAGEPGAGTR
MKLARNMLTFVSYAAAAEAQRLAEACGLDLVALGKVVRHSDSFTGGAGAIMFRNTTAPMEPADPLRPLLEHTRGLGEKDL
SLALALGEVVSVDLPLAQLALQRLAAGLGVPHPDTEPAKET

Sequences:

>Translated_281_residues
MGNQGAPMAKRLLDWPGGLTVFDVRVEAMAPFVEGGATAAASVSDVAEADIISITVFDDAQVSSVITADNGLATHAKPGT
IVAIHSTIADTTAVDLAEKLKPQGIHIVDAPVSGGAAAAAKGELAVMVGADDEAFQRIKEPFSRWASLLIHAGEPGAGTR
MKLARNMLTFVSYAAAAEAQRLAEACGLDLVALGKVVRHSDSFTGGAGAIMFRNTTAPMEPADPLRPLLEHTRGLGEKDL
SLALALGEVVSVDLPLAQLALQRLAAGLGVPHPDTEPAKET
>Mature_280_residues
GNQGAPMAKRLLDWPGGLTVFDVRVEAMAPFVEGGATAAASVSDVAEADIISITVFDDAQVSSVITADNGLATHAKPGTI
VAIHSTIADTTAVDLAEKLKPQGIHIVDAPVSGGAAAAAKGELAVMVGADDEAFQRIKEPFSRWASLLIHAGEPGAGTRM
KLARNMLTFVSYAAAAEAQRLAEACGLDLVALGKVVRHSDSFTGGAGAIMFRNTTAPMEPADPLRPLLEHTRGLGEKDLS
LALALGEVVSVDLPLAQLALQRLAAGLGVPHPDTEPAKET

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=201, Percent_Identity=33.3333333333333, Blast_Score=99, Evalue=6e-21,
Organism=Escherichia coli, GI145693186, Length=274, Percent_Identity=33.2116788321168, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI1790315, Length=257, Percent_Identity=30.7392996108949, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1789092, Length=248, Percent_Identity=33.8709677419355, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786719, Length=221, Percent_Identity=28.9592760180996, Blast_Score=79, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17557316, Length=269, Percent_Identity=30.1115241635688, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24655230, Length=262, Percent_Identity=29.0076335877863, Blast_Score=88, Evalue=8e-18,
Organism=Drosophila melanogaster, GI19922568, Length=262, Percent_Identity=29.0076335877863, Blast_Score=88, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.31

Molecular weight: Translated: 28939; Mature: 28808

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNQGAPMAKRLLDWPGGLTVFDVRVEAMAPFVEGGATAAASVSDVAEADIISITVFDDA
CCCCCCHHHHHHHCCCCCCEEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEECCC
QVSSVITADNGLATHAKPGTIVAIHSTIADTTAVDLAEKLKPQGIHIVDAPVSGGAAAAA
CHHEEEECCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
KGELAVMVGADDEAFQRIKEPFSRWASLLIHAGEPGAGTRMKLARNMLTFVSYAAAAEAQ
CCCEEEEECCCHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RLAEACGLDLVALGKVVRHSDSFTGGAGAIMFRNTTAPMEPADPLRPLLEHTRGLGEKDL
HHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHH
SLALALGEVVSVDLPLAQLALQRLAAGLGVPHPDTEPAKET
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
GNQGAPMAKRLLDWPGGLTVFDVRVEAMAPFVEGGATAAASVSDVAEADIISITVFDDA
CCCCCHHHHHHHCCCCCCEEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEECCC
QVSSVITADNGLATHAKPGTIVAIHSTIADTTAVDLAEKLKPQGIHIVDAPVSGGAAAAA
CHHEEEECCCCCCCCCCCCEEEEEECHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCC
KGELAVMVGADDEAFQRIKEPFSRWASLLIHAGEPGAGTRMKLARNMLTFVSYAAAAEAQ
CCCEEEEECCCHHHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RLAEACGLDLVALGKVVRHSDSFTGGAGAIMFRNTTAPMEPADPLRPLLEHTRGLGEKDL
HHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCHHH
SLALALGEVVSVDLPLAQLALQRLAAGLGVPHPDTEPAKET
HHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-hydroxy-2-methylpropanoate; NAD+

Specific reaction: 3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]