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The map label for this gene is mmsB

Identifier: 148660527

GI number: 148660527

Start: 843653

End: 844537

Strand: Reverse

Name: mmsB

Synonym: MRA_0760

Alternate gene names: 148660527

Gene position: 844537-843653 (Counterclockwise)

Preceding gene: 148660528

Following gene: 148660525

Centisome position: 19.11

GC content: 68.81

Gene sequence:

>885_bases
ATGACGACCATCGCCTTCCTAGGTTTGGGCAACATGGGTGCGCCGATGTCGGCGAATCTGGTTGGTGCGGGCCACGTCGT
GCGTGGATTCGACCCGGCACCCACGGCGGCGTCCGGCGCCGCCGCGCACGGTGTCGCGGTGTTTCGTAGCGCGCCCGAAG
CGGTGGCCGAGGCCGACGTGGTCATCACCATGCTGCCCACCGGCGAGGTGGTCCGGCGCTGCTACACCGACGTGCTGGCC
GCCGCGCGTCCGGCAACGCTGTTCATCGACAGCTCCACGATCTCGGTCACCGATGCCCGTGAGGTGCACGCGCTGGCCGA
ATCGCACGGCATGCTCCAACTGGATGCGCCGGTCTCCGGCGGGGTGAAGGGCGCCGCCGCCGCGACGCTGGCATTCATGG
TCGGCGGCGACGAGTCCACGCTACGGCGGGCACGCCCGGTACTAGAGCCCATGGCGGGCAAGATCATTCACTGCGGCGCC
GCCGGTGCCGGACAGGCCGCCAAGGTGTGCAACAACATGGTGCTGGCGGTGCAGCAGATCGCGATCGCCGAGGCGTTCGT
GCTGGCCGAGAAGCTCGGGCTGTCCGCACAATCGTTGTTCGACGTCATCACCGGCGCGACCGGCAATTGCTGGGCGGTGC
ACACCAATTGCCCGGTGCCGGGCCCGGTGCCCACCTCACCGGCCAACAACGACTTCAAGCCCGGGTTTTCGACCGCGTTG
ATGAACAAGGACCTGGGCCTGGCGATGGATGCGGTGGCCGCCACCGGTGCGACGGCCCCGCTGGGCAGCCACGCCGCCGA
CATCTACGCCAAATTCGCCGCCGACCACGCCGACCTGGACTTCAGCGCGGTGATCCACACGTTGCGCGCGCGAGCAGACG
CATAA

Upstream 100 bases:

>100_bases
CGGGTGCATCGCATCCTGGAAGGGACCAACGAGATCATGCGGCTGGTCATCGGTCGGGCCGAGGCCGCGCGGTTCCGCGC
CACCGTTTAGAGAGGCTCAG

Downstream 100 bases:

>100_bases
GCGCCCATTTCGACACGAAATTGGGCGCTTATGCGTCTGCTCGCCCTCAGAGGTCGCCGGCACACCCATCAGCCCTCGGC
GGCGTCTTTCGGGTCCGACA

Product: 3-hydroxyisobutyrate dehydrogenase MmsB

Products: NA

Alternate protein names: HIBADH

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MTTIAFLGLGNMGAPMSANLVGAGHVVRGFDPAPTAASGAAAHGVAVFRSAPEAVAEADVVITMLPTGEVVRRCYTDVLA
AARPATLFIDSSTISVTDAREVHALAESHGMLQLDAPVSGGVKGAAAATLAFMVGGDESTLRRARPVLEPMAGKIIHCGA
AGAGQAAKVCNNMVLAVQQIAIAEAFVLAEKLGLSAQSLFDVITGATGNCWAVHTNCPVPGPVPTSPANNDFKPGFSTAL
MNKDLGLAMDAVAATGATAPLGSHAADIYAKFAADHADLDFSAVIHTLRARADA

Sequences:

>Translated_294_residues
MTTIAFLGLGNMGAPMSANLVGAGHVVRGFDPAPTAASGAAAHGVAVFRSAPEAVAEADVVITMLPTGEVVRRCYTDVLA
AARPATLFIDSSTISVTDAREVHALAESHGMLQLDAPVSGGVKGAAAATLAFMVGGDESTLRRARPVLEPMAGKIIHCGA
AGAGQAAKVCNNMVLAVQQIAIAEAFVLAEKLGLSAQSLFDVITGATGNCWAVHTNCPVPGPVPTSPANNDFKPGFSTAL
MNKDLGLAMDAVAATGATAPLGSHAADIYAKFAADHADLDFSAVIHTLRARADA
>Mature_293_residues
TTIAFLGLGNMGAPMSANLVGAGHVVRGFDPAPTAASGAAAHGVAVFRSAPEAVAEADVVITMLPTGEVVRRCYTDVLAA
ARPATLFIDSSTISVTDAREVHALAESHGMLQLDAPVSGGVKGAAAATLAFMVGGDESTLRRARPVLEPMAGKIIHCGAA
GAGQAAKVCNNMVLAVQQIAIAEAFVLAEKLGLSAQSLFDVITGATGNCWAVHTNCPVPGPVPTSPANNDFKPGFSTALM
NKDLGLAMDAVAATGATAPLGSHAADIYAKFAADHADLDFSAVIHTLRARADA

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family

Homologues:

Organism=Homo sapiens, GI23308751, Length=292, Percent_Identity=40.4109589041096, Blast_Score=208, Evalue=6e-54,
Organism=Homo sapiens, GI40556376, Length=287, Percent_Identity=25.7839721254355, Blast_Score=91, Evalue=2e-18,
Organism=Escherichia coli, GI145693186, Length=275, Percent_Identity=33.0909090909091, Blast_Score=148, Evalue=5e-37,
Organism=Escherichia coli, GI1790315, Length=297, Percent_Identity=31.6498316498317, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1786719, Length=300, Percent_Identity=31, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789092, Length=291, Percent_Identity=29.8969072164948, Blast_Score=89, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17557316, Length=297, Percent_Identity=38.047138047138, Blast_Score=189, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24655230, Length=291, Percent_Identity=44.3298969072165, Blast_Score=236, Evalue=1e-62,
Organism=Drosophila melanogaster, GI19922568, Length=291, Percent_Identity=44.3298969072165, Blast_Score=236, Evalue=1e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MMSB_MYCBO (P63936)

Other databases:

- EMBL:   BX248336
- RefSeq:   NP_854431.1
- ProteinModelPortal:   P63936
- SMR:   P63936
- EnsemblBacteria:   EBMYCT00000016942
- GeneID:   1092416
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb0773c
- GeneTree:   EBGT00050000015861
- HOGENOM:   HBG729179
- OMA:   PMARNLI
- ProtClustDB:   CLSK790712
- BioCyc:   MBOV233413:MB0773C-MONOMER
- BRENDA:   1.1.1.31
- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR011548
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.1040.10
- PANTHER:   PTHR22981
- TIGRFAMs:   TIGR01692

Pfam domain/function: PF03446 NAD_binding_2; SSF48179 6DGDH_C_like

EC number: =1.1.1.31

Molecular weight: Translated: 29679; Mature: 29548

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: ACT_SITE 168-168 BINDING 93-93 BINDING 243-243

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIAFLGLGNMGAPMSANLVGAGHVVRGFDPAPTAASGAAAHGVAVFRSAPEAVAEADV
CCEEEEEECCCCCCCCCCCEEECCCEEECCCCCCCCCCCCHHHHHHHHHCCCHHHHCCCE
VITMLPTGEVVRRCYTDVLAAARPATLFIDSSTISVTDAREVHALAESHGMLQLDAPVSG
EEEECCCHHHHHHHHHHHHHHCCCCEEEEECCEEEECCHHHHHHHHHHCCEEEECCCCCC
GVKGAAAATLAFMVGGDESTLRRARPVLEPMAGKIIHCGAAGAGQAAKVCNNMVLAVQQI
CCCHHHHHEEEEEECCCHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHH
AIAEAFVLAEKLGLSAQSLFDVITGATGNCWAVHTNCPVPGPVPTSPANNDFKPGFSTAL
HHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHH
MNKDLGLAMDAVAATGATAPLGSHAADIYAKFAADHADLDFSAVIHTLRARADA
HCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTIAFLGLGNMGAPMSANLVGAGHVVRGFDPAPTAASGAAAHGVAVFRSAPEAVAEADV
CEEEEEECCCCCCCCCCCEEECCCEEECCCCCCCCCCCCHHHHHHHHHCCCHHHHCCCE
VITMLPTGEVVRRCYTDVLAAARPATLFIDSSTISVTDAREVHALAESHGMLQLDAPVSG
EEEECCCHHHHHHHHHHHHHHCCCCEEEEECCEEEECCHHHHHHHHHHCCEEEECCCCCC
GVKGAAAATLAFMVGGDESTLRRARPVLEPMAGKIIHCGAAGAGQAAKVCNNMVLAVQQI
CCCHHHHHEEEEEECCCHHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHH
AIAEAFVLAEKLGLSAQSLFDVITGATGNCWAVHTNCPVPGPVPTSPANNDFKPGFSTAL
HHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHH
MNKDLGLAMDAVAATGATAPLGSHAADIYAKFAADHADLDFSAVIHTLRARADA
HCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972