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The map label for this gene is pnp [C]

Identifier: 148660304

GI number: 148660304

Start: 627767

End: 628561

Strand: Direct

Name: pnp [C]

Synonym: MRA_0542

Alternate gene names: 148660304

Gene position: 627767-628561 (Clockwise)

Preceding gene: 148660301

Following gene: 148660305

Centisome position: 14.2

GC content: 66.67

Gene sequence:

>795_bases
ATGCACAACAATGGGCGCATGCTCGGAGTGATCGGCGGCAGCGGCTTCTACACCTTCTTTGGGTCGGACACCCGCACAGT
CAATTCGGACACCCCCTACGGTCAACCCAGCGCCCCGATCACGATCGGCACCATCGGGGTGCACGACGTCGCGTTCTTGC
CCCGCCACGGCGCCCATCACCAGTACTCGGCGCACGCCGTGCCGTATCGGGCCAACATGTGGGCGCTGCGCGCGCTTGGT
GTGCGGCGGGTCTTCGGGCCGTGTGCGGTCGGCAGCCTGGACCCTGAACTCGAGCCCGGCGCGGTCGTGGTGCCCGATCA
GCTGGTCGACCGCACCAGCGGCCGCGCCGACACCTATTTCGACTTCGGCGGTGTCCATGCCGCCTTCGCCGATCCGTACT
GCCCCACGCTGCGGGCCGCGGTGACCGGCCTGCCCGGTGTTGTCGACGGCGGCACCATGGTGGTGATCCAGGGTCCGCGG
TTTTCCACCCGCGCGGAAAGCCAGTGGTTCGCCGCTGCCGGGTGCAATCTGGTCAACATGACCGGCTATCCCGAGGCGGT
GCTGGCTCGCGAACTCGAATTATGCTACGCAGCAATCGCTTTGGTGACAGATGTGGATGCCGGCGTCGCTGCTGGCGATG
GCGTGAAAGCCGCCGACGTGTTCGCCGCATTCGGGGAGAACATCGAACTGCTCAAAAGGCTGGTGCGGGCCGCCATCGAT
CGGGTCGCCGACGAGCGCACGTGCACGCACTGTCAACACCACGCCGGTGTTCCGTTGCCGTTCGAGCTGCCATGA

Upstream 100 bases:

>100_bases
GCCGGTGCCGGCAACCACTGGCGCGATCGCGTTCGGCAGCGTTCGGGGCCGCGCGCCGGAGACCCACTGTGCGAAACTGG
CCACCAGGGCATCCTGCCCT

Downstream 100 bases:

>100_bases
GGGTGCTGCTGACCGGCGCGGCCGGCTTCATCGGGTCGCGCGTGGATGCGGCGTTACGGGCTGCGGGTCACGACGTGGTG
GGCGTCGACGCGCTGCTGCC

Product: 5'-methylthioadenosine phosphorylase

Products: ribose-1-phosphate; xanthine [C]

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MHNNGRMLGVIGGSGFYTFFGSDTRTVNSDTPYGQPSAPITIGTIGVHDVAFLPRHGAHHQYSAHAVPYRANMWALRALG
VRRVFGPCAVGSLDPELEPGAVVVPDQLVDRTSGRADTYFDFGGVHAAFADPYCPTLRAAVTGLPGVVDGGTMVVIQGPR
FSTRAESQWFAAAGCNLVNMTGYPEAVLARELELCYAAIALVTDVDAGVAAGDGVKAADVFAAFGENIELLKRLVRAAID
RVADERTCTHCQHHAGVPLPFELP

Sequences:

>Translated_264_residues
MHNNGRMLGVIGGSGFYTFFGSDTRTVNSDTPYGQPSAPITIGTIGVHDVAFLPRHGAHHQYSAHAVPYRANMWALRALG
VRRVFGPCAVGSLDPELEPGAVVVPDQLVDRTSGRADTYFDFGGVHAAFADPYCPTLRAAVTGLPGVVDGGTMVVIQGPR
FSTRAESQWFAAAGCNLVNMTGYPEAVLARELELCYAAIALVTDVDAGVAAGDGVKAADVFAAFGENIELLKRLVRAAID
RVADERTCTHCQHHAGVPLPFELP
>Mature_264_residues
MHNNGRMLGVIGGSGFYTFFGSDTRTVNSDTPYGQPSAPITIGTIGVHDVAFLPRHGAHHQYSAHAVPYRANMWALRALG
VRRVFGPCAVGSLDPELEPGAVVVPDQLVDRTSGRADTYFDFGGVHAAFADPYCPTLRAAVTGLPGVVDGGTMVVIQGPR
FSTRAESQWFAAAGCNLVNMTGYPEAVLARELELCYAAIALVTDVDAGVAAGDGVKAADVFAAFGENIELLKRLVRAAID
RVADERTCTHCQHHAGVPLPFELP

Specific function: The Nucleoside Phosphorylases Catalyze The Phosphorolytic Breakdown Of The N-Glycosidic Bond In The Nucleoside Molecule, With The Formation Of The Corresponding Free Bases And Pentose-1-Phosphate. This Protein Can Degrade All Purine Nucleosides Except Ade

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI47132622, Length=247, Percent_Identity=37.246963562753, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI71980569, Length=247, Percent_Identity=39.6761133603239, Blast_Score=152, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6323045, Length=243, Percent_Identity=35.8024691358025, Blast_Score=140, Evalue=2e-34,
Organism=Drosophila melanogaster, GI20130079, Length=252, Percent_Identity=38.0952380952381, Blast_Score=156, Evalue=1e-38,
Organism=Drosophila melanogaster, GI221459247, Length=227, Percent_Identity=33.0396475770925, Blast_Score=129, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010044
- InterPro:   IPR000845
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 27964; Mature: 27964

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNNGRMLGVIGGSGFYTFFGSDTRTVNSDTPYGQPSAPITIGTIGVHDVAFLPRHGAHH
CCCCCEEEEEEECCCEEEEECCCCEEECCCCCCCCCCCCEEEEECCCHHHEECCCCCCCC
QYSAHAVPYRANMWALRALGVRRVFGPCAVGSLDPELEPGAVVVPDQLVDRTSGRADTYF
CCCCCCCCEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCHHHHHCCCCCCCCEE
DFGGVHAAFADPYCPTLRAAVTGLPGVVDGGTMVVIQGPRFSTRAESQWFAAAGCNLVNM
ECCCEEEECCCCCCHHHHHHHHCCCCEECCCEEEEEECCCCCCCCCCCEEECCCCCEEEE
TGYPEAVLARELELCYAAIALVTDVDAGVAAGDGVKAADVFAAFGENIELLKRLVRAAID
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
RVADERTCTHCQHHAGVPLPFELP
HHHCHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MHNNGRMLGVIGGSGFYTFFGSDTRTVNSDTPYGQPSAPITIGTIGVHDVAFLPRHGAHH
CCCCCEEEEEEECCCEEEEECCCCEEECCCCCCCCCCCCEEEEECCCHHHEECCCCCCCC
QYSAHAVPYRANMWALRALGVRRVFGPCAVGSLDPELEPGAVVVPDQLVDRTSGRADTYF
CCCCCCCCEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCHHHHHCCCCCCCCEE
DFGGVHAAFADPYCPTLRAAVTGLPGVVDGGTMVVIQGPRFSTRAESQWFAAAGCNLVNM
ECCCEEEECCCCCCHHHHHHHHCCCCEECCCEEEEEECCCCCCCCCCCEEECCCCCEEEE
TGYPEAVLARELELCYAAIALVTDVDAGVAAGDGVKAADVFAAFGENIELLKRLVRAAID
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHH
RVADERTCTHCQHHAGVPLPFELP
HHHCHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: xanthosine; phosphate [C]

Specific reaction: xanthosine + phosphate = ribose-1-phosphate + xanthine [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]