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The map label for this gene is hemL

Identifier: 148660293

GI number: 148660293

Start: 616145

End: 617533

Strand: Direct

Name: hemL

Synonym: MRA_0531

Alternate gene names: 148660293

Gene position: 616145-617533 (Clockwise)

Preceding gene: 148660291

Following gene: 148660294

Centisome position: 13.94

GC content: 69.76

Gene sequence:

>1389_bases
ATGGGCAGCACGGAACAGGCCACCTCGCGGGTAAGGGGAGCCGCGCGCACATCGGCGCAGCTGTTCGAGGCCGCATGCAG
CGTCATACCCGGCGGAGTGAACTCCCCGGTGCGGGCGTTCACGGCGGTGGGCGGCACCCCGCGCTTCATTACCGAAGCCC
ACGGCTGCTGGTTGATTGACGCCGACGGCAACCGCTACGTAGACCTGGTCTGCTCATGGGGCCCGATGATCCTCGGTCAC
GCGCATCCGGCCGTCGTCGAGGCAGTGGCCAAGGCCGCAGCCCGCGGCCTGTCCTTCGGGGCCCCGACTCCCGCCGAAAC
CCAACTAGCCGGCGAGATCATCGGCCGGGTAGCTCCCGTCGAGCGGATACGGCTGGTGAACTCCGGCACCGAGGCCACTA
TGAGCGCCGTGCGGCTGGCCCGCGGATTCACCGGCCGGGCCAAGATCGTCAAGTTCTCCGGCTGCTACCACGGACACGTC
GACGCATTGCTCGCCGACGCGGGTTCGGGAGTGGCCACCCTGGGCTTATGTGACGACCCCCAGCGCCCGGCTTCGCCGCG
CTCGCAATCGTCACGGGGCCTGCCGTCCTCCCCCGGGGTCACTGGCGCCGCGGCAGCCGACACGATCGTGTTGCCCTACA
ACGACATCGATGCCGTACAGCAGACCTTCGCCCGGTTCGGCGAGCAGATCGCCGCCGTAATCACCGAGGCCAGCCCCGGC
AACATGGGAGTCGTCCCGCCCGGGCCCGGCTTCAACGCGGCGCTGCGCGCGATCACCGCCGAGCACGGCGCCCTGCTCAT
CCTCGACGAGGTGATGACCGGGTTCCGGGTCAGCCGAAGTGGTTGGTACGGAATCGATCCGGTGCCCGCTGACCTGTTCG
CCTTCGGCAAGGTGATGAGCGGCGGGATGCCCGCCGCCGCGTTCGGCGGGCGCGCCGAGGTGATGCAGCGGCTGGCGCCG
CTGGGGCCGGTGTATCAGGCCGGCACGTTGTCGGGTAACCCGGTGGCGGTTGCCGCCGGGCTGGCAACGCTGCGGGCCGC
CGACGACGCGGTCTACACCGCATTGGACGCCAACGCTGACCGCCTGGCCGGCCTGCTCTCCGAGGCACTGACGGATGCCG
TTGTGCCACACCAGATTTCGCGGGCAGGCAATATGCTCAGTGTGTTCTTCGGCGAAACACCGGTGACCGACTTCGCGTCC
GCGCGGGCCAGCCAGACCTGGCGTTATCCAGCGTTCTTTCATGCCATGCTGGACGCCGGTGTCTACCCGCCGTGCAGTGC
CTTCGAGGCATGGTTCGTCTCGGCCGCTTTGGACGACGCGGCGTTCGGCCGGATCGCCAACGCGCTGCCCGCCGCGGCCC
GAGCGGCGGCCCAGGAAAGGCCCGCCTGA

Upstream 100 bases:

>100_bases
CTCGTGGACCGGCCGCGGCATCAAGCAAACCTCTGGTGGCTGCGGCAAACTCTTACACCCTGTAGTTGAGCGACCTGGGC
AGGCTGGAACACTAGTCGTC

Downstream 100 bases:

>100_bases
TGCCCGAGGAAACCCAAGTCCACGTGGTGCGCCACGGTGAGGTGCACAACCCTACCGGCATCCTGTACGGGCGGCTGCCC
GGATTCCACCTGTCCGCAAC

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MGSTEQATSRVRGAARTSAQLFEAACSVIPGGVNSPVRAFTAVGGTPRFITEAHGCWLIDADGNRYVDLVCSWGPMILGH
AHPAVVEAVAKAAARGLSFGAPTPAETQLAGEIIGRVAPVERIRLVNSGTEATMSAVRLARGFTGRAKIVKFSGCYHGHV
DALLADAGSGVATLGLCDDPQRPASPRSQSSRGLPSSPGVTGAAAADTIVLPYNDIDAVQQTFARFGEQIAAVITEASPG
NMGVVPPGPGFNAALRAITAEHGALLILDEVMTGFRVSRSGWYGIDPVPADLFAFGKVMSGGMPAAAFGGRAEVMQRLAP
LGPVYQAGTLSGNPVAVAAGLATLRAADDAVYTALDANADRLAGLLSEALTDAVVPHQISRAGNMLSVFFGETPVTDFAS
ARASQTWRYPAFFHAMLDAGVYPPCSAFEAWFVSAALDDAAFGRIANALPAAARAAAQERPA

Sequences:

>Translated_462_residues
MGSTEQATSRVRGAARTSAQLFEAACSVIPGGVNSPVRAFTAVGGTPRFITEAHGCWLIDADGNRYVDLVCSWGPMILGH
AHPAVVEAVAKAAARGLSFGAPTPAETQLAGEIIGRVAPVERIRLVNSGTEATMSAVRLARGFTGRAKIVKFSGCYHGHV
DALLADAGSGVATLGLCDDPQRPASPRSQSSRGLPSSPGVTGAAAADTIVLPYNDIDAVQQTFARFGEQIAAVITEASPG
NMGVVPPGPGFNAALRAITAEHGALLILDEVMTGFRVSRSGWYGIDPVPADLFAFGKVMSGGMPAAAFGGRAEVMQRLAP
LGPVYQAGTLSGNPVAVAAGLATLRAADDAVYTALDANADRLAGLLSEALTDAVVPHQISRAGNMLSVFFGETPVTDFAS
ARASQTWRYPAFFHAMLDAGVYPPCSAFEAWFVSAALDDAAFGRIANALPAAARAAAQERPA
>Mature_461_residues
GSTEQATSRVRGAARTSAQLFEAACSVIPGGVNSPVRAFTAVGGTPRFITEAHGCWLIDADGNRYVDLVCSWGPMILGHA
HPAVVEAVAKAAARGLSFGAPTPAETQLAGEIIGRVAPVERIRLVNSGTEATMSAVRLARGFTGRAKIVKFSGCYHGHVD
ALLADAGSGVATLGLCDDPQRPASPRSQSSRGLPSSPGVTGAAAADTIVLPYNDIDAVQQTFARFGEQIAAVITEASPGN
MGVVPPGPGFNAALRAITAEHGALLILDEVMTGFRVSRSGWYGIDPVPADLFAFGKVMSGGMPAAAFGGRAEVMQRLAPL
GPVYQAGTLSGNPVAVAAGLATLRAADDAVYTALDANADRLAGLLSEALTDAVVPHQISRAGNMLSVFFGETPVTDFASA
RASQTWRYPAFFHAMLDAGVYPPCSAFEAWFVSAALDDAAFGRIANALPAAARAAAQERPA

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=338, Percent_Identity=27.5147928994083, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI37574042, Length=362, Percent_Identity=27.0718232044199, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI226442709, Length=331, Percent_Identity=27.190332326284, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI226442705, Length=365, Percent_Identity=25.4794520547945, Blast_Score=90, Evalue=6e-18,
Organism=Homo sapiens, GI284507298, Length=257, Percent_Identity=28.4046692607004, Blast_Score=75, Evalue=9e-14,
Organism=Escherichia coli, GI1786349, Length=446, Percent_Identity=50.8968609865471, Blast_Score=407, Evalue=1e-114,
Organism=Escherichia coli, GI1789016, Length=368, Percent_Identity=30.4347826086957, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1787560, Length=384, Percent_Identity=30.7291666666667, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI145693181, Length=300, Percent_Identity=30.3333333333333, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789759, Length=301, Percent_Identity=28.5714285714286, Blast_Score=95, Evalue=9e-21,
Organism=Escherichia coli, GI1788044, Length=298, Percent_Identity=31.5436241610738, Blast_Score=93, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71992977, Length=377, Percent_Identity=27.5862068965517, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25144271, Length=310, Percent_Identity=27.0967741935484, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI32564660, Length=329, Percent_Identity=24.9240121580547, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21357415, Length=340, Percent_Identity=23.8235294117647, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_MYCBO (P63507)

Other databases:

- EMBL:   BX248335
- RefSeq:   NP_854199.1
- ProteinModelPortal:   P63507
- SMR:   P63507
- EnsemblBacteria:   EBMYCT00000015082
- GeneID:   1091603
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb0537
- GeneTree:   EBGT00050000015339
- HOGENOM:   HBG725944
- OMA:   VGCFGGK
- ProtClustDB:   PRK00062
- BioCyc:   MBOV233413:MB0537-MONOMER
- BRENDA:   5.4.3.8
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 47516; Mature: 47385

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSTEQATSRVRGAARTSAQLFEAACSVIPGGVNSPVRAFTAVGGTPRFITEAHGCWLID
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHCCCCCEEEEE
ADGNRYVDLVCSWGPMILGHAHPAVVEAVAKAAARGLSFGAPTPAETQLAGEIIGRVAPV
CCCCEEEEEEECCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCH
ERIRLVNSGTEATMSAVRLARGFTGRAKIVKFSGCYHGHVDALLADAGSGVATLGLCDDP
HHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHCCCCCEEEEECCCCC
QRPASPRSQSSRGLPSSPGVTGAAAADTIVLPYNDIDAVQQTFARFGEQIAAVITEASPG
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCC
NMGVVPPGPGFNAALRAITAEHGALLILDEVMTGFRVSRSGWYGIDPVPADLFAFGKVMS
CEECCCCCCCHHHHHHHHHHCCCCEEEHHHHHHCEEECCCCCCCCCCCCHHHHHHHHHHC
GGMPAAAFGGRAEVMQRLAPLGPVYQAGTLSGNPVAVAAGLATLRAADDAVYTALDANAD
CCCCHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCHHEEEECCCHH
RLAGLLSEALTDAVVPHQISRAGNMLSVFFGETPVTDFASARASQTWRYPAFFHAMLDAG
HHHHHHHHHHHHHHCHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHCC
VYPPCSAFEAWFVSAALDDAAFGRIANALPAAARAAAQERPA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GSTEQATSRVRGAARTSAQLFEAACSVIPGGVNSPVRAFTAVGGTPRFITEAHGCWLID
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHCCCCCEEEEE
ADGNRYVDLVCSWGPMILGHAHPAVVEAVAKAAARGLSFGAPTPAETQLAGEIIGRVAPV
CCCCEEEEEEECCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCH
ERIRLVNSGTEATMSAVRLARGFTGRAKIVKFSGCYHGHVDALLADAGSGVATLGLCDDP
HHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHCCCCCEEEEECCCCC
QRPASPRSQSSRGLPSSPGVTGAAAADTIVLPYNDIDAVQQTFARFGEQIAAVITEASPG
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCC
NMGVVPPGPGFNAALRAITAEHGALLILDEVMTGFRVSRSGWYGIDPVPADLFAFGKVMS
CEECCCCCCCHHHHHHHHHHCCCCEEEHHHHHHCEEECCCCCCCCCCCCHHHHHHHHHHC
GGMPAAAFGGRAEVMQRLAPLGPVYQAGTLSGNPVAVAAGLATLRAADDAVYTALDANAD
CCCCHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHCCCHHEEEECCCHH
RLAGLLSEALTDAVVPHQISRAGNMLSVFFGETPVTDFASARASQTWRYPAFFHAMLDAG
HHHHHHHHHHHHHHCHHHHHHCCCEEEEEECCCCCHHHHHHHHCCCCCCHHHHHHHHHCC
VYPPCSAFEAWFVSAALDDAAFGRIANALPAAARAAAQERPA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972