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The map label for this gene is hemB

Identifier: 148660281

GI number: 148660281

Start: 605912

End: 606901

Strand: Direct

Name: hemB

Synonym: MRA_0519

Alternate gene names: 148660281

Gene position: 605912-606901 (Clockwise)

Preceding gene: 148660280

Following gene: 148660282

Centisome position: 13.71

GC content: 67.17

Gene sequence:

>990_bases
ATGAGCATGAGTTCCTATCCGCGGCAGCGACCGCGCCGGCTCCGCTCCACCGTCGCGATGCGCCGTCTGGTTGCGCAAAC
CTCGTTGGAGCCAAGGCATTTGGTGCTGCCGATGTTCGTTGCCGACGGCATTGACGAGCCGCGGCCGATTACCTCCATGC
CGGGCGTGGTACAGCACACCCGGGATTCGCTACGTAGGGCCGCGGCAGCCGCGGTGGCCGCCGGCGTGGGTGGGCTGATG
CTTTTCGGCGTGCCGCGCGACCAGGACAAGGACGGTGTCGGTTCGGCGGGCATCGACCCCGACGGGATCCTCAACGTCGC
CCTTCGCGATCTGGCCAAGGACCTGGGTGAGGCCACGGTGTTGATGGCCGACACCTGTCTGGACGAGTTCACCGACCACG
GGCACTGCGGTGTGCTCGATGACCGGGGCCGGGTCGATAACGACGCCACCGTGGCCCGCTATGTGGAACTGGCTGTGGCG
CAAGCGGAATCGGGCGCCCACGTGGTCGGACCCAGTGGGATGATGGATGGCCAGGTAGCCGCGATCCGGGACGGTTTGGA
CGCCGCCGGCTACATCGATGTGGTGATCTTGGCCTACGCCGCGAAGTTTGCTTCGGCGTTCTACGGCCCGTTCCGCGAGG
CGGTGAGCTCTAGCCTGTCCGGGGATCGGCGCACCTACCAGCAGGAGCCGGGCAACGCCGCCGAGGCGCTGCGTGAGATC
GAGCTCGATCTCGACGAAGGCGCCGACATTGTGATGGTCAAACCCGCGATGGGCTACCTCGATGTGGTGGCGGCCGCGGC
GGACGTCTCGCCGGTCCCGGTGGCCGCCTATCAGGTCTCGGGAGAGTACGCGATGATTCGTGCGGCGGCGGCCAATAATT
GGATCGATGAGCGTGCCGCGGTGCTAGAGTCGCTGACCGGTATCCGGCGTGCCGGCGCCGACATCGTGCTCACCTACTGG
GCGGTAGACGCGGCGGGCTGGCTTACGTGA

Upstream 100 bases:

>100_bases
AGCCGCAGGCGCTAGTGGCCCACCCTCGTCAGGTGAGCGTGCGTGTCTGTACACCGACACGCCGACCGAGCTGGCATTTT
GCGTACGCTCGCGGCTACGA

Downstream 100 bases:

>100_bases
CGGAGGCCTGACATGACACCAACCGGGGATACCAAGCCCAAGTTGTTGTTCTACGAACCCGGCGCGAGCTGGTACTGGGT
GCTGACTGGTCCGCTTGCGG

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MSMSSYPRQRPRRLRSTVAMRRLVAQTSLEPRHLVLPMFVADGIDEPRPITSMPGVVQHTRDSLRRAAAAAVAAGVGGLM
LFGVPRDQDKDGVGSAGIDPDGILNVALRDLAKDLGEATVLMADTCLDEFTDHGHCGVLDDRGRVDNDATVARYVELAVA
QAESGAHVVGPSGMMDGQVAAIRDGLDAAGYIDVVILAYAAKFASAFYGPFREAVSSSLSGDRRTYQQEPGNAAEALREI
ELDLDEGADIVMVKPAMGYLDVVAAAADVSPVPVAAYQVSGEYAMIRAAAANNWIDERAAVLESLTGIRRAGADIVLTYW
AVDAAGWLT

Sequences:

>Translated_329_residues
MSMSSYPRQRPRRLRSTVAMRRLVAQTSLEPRHLVLPMFVADGIDEPRPITSMPGVVQHTRDSLRRAAAAAVAAGVGGLM
LFGVPRDQDKDGVGSAGIDPDGILNVALRDLAKDLGEATVLMADTCLDEFTDHGHCGVLDDRGRVDNDATVARYVELAVA
QAESGAHVVGPSGMMDGQVAAIRDGLDAAGYIDVVILAYAAKFASAFYGPFREAVSSSLSGDRRTYQQEPGNAAEALREI
ELDLDEGADIVMVKPAMGYLDVVAAAADVSPVPVAAYQVSGEYAMIRAAAANNWIDERAAVLESLTGIRRAGADIVLTYW
AVDAAGWLT
>Mature_328_residues
SMSSYPRQRPRRLRSTVAMRRLVAQTSLEPRHLVLPMFVADGIDEPRPITSMPGVVQHTRDSLRRAAAAAVAAGVGGLML
FGVPRDQDKDGVGSAGIDPDGILNVALRDLAKDLGEATVLMADTCLDEFTDHGHCGVLDDRGRVDNDATVARYVELAVAQ
AESGAHVVGPSGMMDGQVAAIRDGLDAAGYIDVVILAYAAKFASAFYGPFREAVSSSLSGDRRTYQQEPGNAAEALREIE
LDLDEGADIVMVKPAMGYLDVVAAAADVSPVPVAAYQVSGEYAMIRAAAANNWIDERAAVLESLTGIRRAGADIVLTYWA
VDAAGWLT

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family

Homologues:

Organism=Homo sapiens, GI189083849, Length=308, Percent_Identity=43.5064935064935, Blast_Score=236, Evalue=2e-62,
Organism=Escherichia coli, GI87081728, Length=317, Percent_Identity=47.6340694006309, Blast_Score=276, Evalue=1e-75,
Organism=Saccharomyces cerevisiae, GI6321398, Length=339, Percent_Identity=36.8731563421829, Blast_Score=217, Evalue=3e-57,
Organism=Drosophila melanogaster, GI21358291, Length=318, Percent_Identity=40.5660377358491, Blast_Score=221, Evalue=7e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM2_MYCTU (O33357)

Other databases:

- EMBL:   BX842573
- EMBL:   AE000516
- PIR:   E70509
- RefSeq:   NP_215026.1
- RefSeq:   NP_334942.1
- ProteinModelPortal:   O33357
- SMR:   O33357
- EnsemblBacteria:   EBMYCT00000001282
- EnsemblBacteria:   EBMYCT00000072421
- GeneID:   887312
- GeneID:   924242
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT0533
- KEGG:   mtu:Rv0512
- TIGR:   MT0533
- TubercuList:   Rv0512
- GeneTree:   EBGT00050000016458
- HOGENOM:   HBG285270
- OMA:   ADLCFCE
- ProtClustDB:   PRK09283
- BRENDA:   4.2.1.24
- InterPro:   IPR001731
- InterPro:   IPR013785
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR11458
- PIRSF:   PIRSF001415
- PRINTS:   PR00144

Pfam domain/function: PF00490 ALAD

EC number: =4.2.1.24

Molecular weight: Translated: 34872; Mature: 34741

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: ACT_SITE 202-202 ACT_SITE 254-254 BINDING 212-212 BINDING 223-223 BINDING 280-280 BINDING 319-319

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMSSYPRQRPRRLRSTVAMRRLVAQTSLEPRHLVLPMFVADGIDEPRPITSMPGVVQHT
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHCCCCCCCCCCCCCHHHHHH
RDSLRRAAAAAVAAGVGGLMLFGVPRDQDKDGVGSAGIDPDGILNVALRDLAKDLGEATV
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHEE
LMADTCLDEFTDHGHCGVLDDRGRVDNDATVARYVELAVAQAESGAHVVGPSGMMDGQVA
HHHHHHHHHHCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHH
AIRDGLDAAGYIDVVILAYAAKFASAFYGPFREAVSSSLSGDRRTYQQEPGNAAEALREI
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHH
ELDLDEGADIVMVKPAMGYLDVVAAAADVSPVPVAAYQVSGEYAMIRAAAANNWIDERAA
HCCCCCCCCEEEECCCCHHHHHHHHHCCCCCCCEEEEEECCCEEEEEEHHHCCHHHHHHH
VLESLTGIRRAGADIVLTYWAVDAAGWLT
HHHHHHHHHHCCCCEEEEEEEHHHCCCCC
>Mature Secondary Structure 
SMSSYPRQRPRRLRSTVAMRRLVAQTSLEPRHLVLPMFVADGIDEPRPITSMPGVVQHT
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHCCCCCCCCCCCCCHHHHHH
RDSLRRAAAAAVAAGVGGLMLFGVPRDQDKDGVGSAGIDPDGILNVALRDLAKDLGEATV
HHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCHHEE
LMADTCLDEFTDHGHCGVLDDRGRVDNDATVARYVELAVAQAESGAHVVGPSGMMDGQVA
HHHHHHHHHHCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHH
AIRDGLDAAGYIDVVILAYAAKFASAFYGPFREAVSSSLSGDRRTYQQEPGNAAEALREI
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHH
ELDLDEGADIVMVKPAMGYLDVVAAAADVSPVPVAAYQVSGEYAMIRAAAANNWIDERAA
HCCCCCCCCEEEECCCCHHHHHHHHHCCCCCCCEEEEEECCCEEEEEEHHHCCHHHHHHH
VLESLTGIRRAGADIVLTYWAVDAAGWLT
HHHHHHHHHHCCCCEEEEEEEHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036