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The map label for this gene is hemD [H]

Identifier: 148660280

GI number: 148660280

Start: 604129

End: 605826

Strand: Direct

Name: hemD [H]

Synonym: MRA_0518

Alternate gene names: 148660280

Gene position: 604129-605826 (Clockwise)

Preceding gene: 148660279

Following gene: 148660281

Centisome position: 13.67

GC content: 70.08

Gene sequence:

>1698_bases
ATGACGCGAGGGCGTAAGCCGAGACCGGGCCGCATCGTTTTCGTGGGCTCCGGTCCGGGCGACCCCGGCTTGCTTACGAC
ACGGGCTGCCGCGGTGCTGGCCAACGCCGCGCTGGTGTTCACCGATCCCGACGTACCGGAGCCGGTGGTGGCGCTGATCG
GCACGGATCTGCCCCCCGTGTCCGGCCCGGCGCCCGCCGAGCCGGTTGCCGGGAACGGCGATGCGGCCGGCGGAGGAAGT
GCGCAGGAACACGGCCGGGCCGCGTCCGCGGTAGTCTCCGGTGGTCCTGACATCCGCCCGGCGCTGGGCGATCCCGCCGA
TGTGGCCAAGACGCTGACCGCCGAGGCCCGTTCGGGTGTCGACGTGGTGCGGCTGGTGGCGGGCGATCCGCTCACGGTGG
ATGCGGTAATCAGCGAGGTGAACGCCGTCGCACGCACCCACCTGCACATCGAAATCGTGCCCGGCCTGGCCGCCAGCAGC
GCGGTCCCGACCTATGCCGGGTTGCCGCTGGGTTCGTCGCACACCGTCGCCGACGTGCGTATCGACCCCGAAAACACCGA
CTGGGACGCGCTGGCTGCCGCACCCGGGCCGCTGATCCTGCAGGCCACCGCATCGCATCTAGCCGAATCGGCCCGCAGCC
TGATCGATCACCAGCTGGCCGAGTCCACTCCGTGCGTGGTGACCGCACACGGCACCACCTGTCAGCAGCGTTCGGTCGAG
ACCACACTTCAGGGATTGACCGACCCGGCCGTCCTGGGCGCTACCGACCCCGCGTGCTCCGCAAACGGGAGGGACTCCCA
GGCCGGACCGCTGATAGTGACCATCGGCAAGACGGTGACCAGTCGGGCAAAGCTGAACTGGTGGGAGAGCCGCGCCCTCT
ACGGCTGGACGGTGTTGGTGCCGCGCACCAAGGACCAGGCCGGCGAGATGAGCGAGCGGCTCACGTCGTACGGCGCGCTG
CCGGTGGAGGTGCCGACCATCGCCGTCGAGCCGCCGCGCAGCCCCGCGCAGATGGAGCGCGCCGTCAAGGGCCTGGTCGA
TGGCCGATTCCAGTGGATCGTGTTCACCTCCACCAACGCGGTGCGTGCGGTGTGGGAGAAGTTCGGCGAGTTCGGTCTGG
ATGCCCGCGCGTTCTCCGGGGTGAAGATCGCCTGTGTCGGCGAGTCGACGGCCGACCGGGTGCGCGCCTTCGGAATCAGT
CCCGAGCTGGTGCCCTCCGGGGAGCAGTCCTCGCTTGGCTTGCTAGACGACTTCCCGCCCTACGACAGCGTTTTCGACCC
GGTGAACCGGGTTTTGCTGCCGCGCGCCGACATCGCCACCGAAACGCTGGCCGAGGGACTGCGAGAGCGTGGCTGGGAGA
TCGAGGACGTCACCGCCTACCGGACCGTGCGGGCCGCGCCGCCGCCGGCCACTACCCGGGAAATGATCAAGACGGGCGGG
TTTGACGCGGTATGTTTCACCTCCAGCTCGACGGTGCGAAACCTGGTCGGCATCGCCGGCAAGCCGCACGCGCGGACGAT
CATCGCCTGCATAGGGCCAAAGACCGCCGAGACCGCAGCCGAGTTCGGCTTGCGGGTCGATGTCCAGCCGGACACCGCCG
CCATCGGCCCGCTGGTCGATGCGCTGGCCGAGCATGCCGCCCGGTTGCGCGCTGAGGGTGCGCTGCCCCCGCCGCGCAAG
AAGAGCCGCAGGCGCTAG

Upstream 100 bases:

>100_bases
GGCTCTCGGTCGCCGCGGAGCTGTTCGAGCTGGGCGCCCGGGAGCTGATGTGGGGAGTGCGGCATTAGCCCGCATGAAGA
AGTGACTGGGAGTGACAATC

Downstream 100 bases:

>100_bases
TGGCCCACCCTCGTCAGGTGAGCGTGCGTGTCTGTACACCGACACGCCGACCGAGCTGGCATTTTGCGTACGCTCGCGGC
TACGAATGAGCATGAGTTCC

Product: uroporphyrin-III C-methyltransferase/uroporphyrinogen-III synthase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Uroporphyrinogen-III synthase; UROS; Hydroxymethylbilane hydrolyase [cyclizing]; Uroporphyrinogen-III cosynthase [H]

Number of amino acids: Translated: 565; Mature: 564

Protein sequence:

>565_residues
MTRGRKPRPGRIVFVGSGPGDPGLLTTRAAAVLANAALVFTDPDVPEPVVALIGTDLPPVSGPAPAEPVAGNGDAAGGGS
AQEHGRAASAVVSGGPDIRPALGDPADVAKTLTAEARSGVDVVRLVAGDPLTVDAVISEVNAVARTHLHIEIVPGLAASS
AVPTYAGLPLGSSHTVADVRIDPENTDWDALAAAPGPLILQATASHLAESARSLIDHQLAESTPCVVTAHGTTCQQRSVE
TTLQGLTDPAVLGATDPACSANGRDSQAGPLIVTIGKTVTSRAKLNWWESRALYGWTVLVPRTKDQAGEMSERLTSYGAL
PVEVPTIAVEPPRSPAQMERAVKGLVDGRFQWIVFTSTNAVRAVWEKFGEFGLDARAFSGVKIACVGESTADRVRAFGIS
PELVPSGEQSSLGLLDDFPPYDSVFDPVNRVLLPRADIATETLAEGLRERGWEIEDVTAYRTVRAAPPPATTREMIKTGG
FDAVCFTSSSTVRNLVGIAGKPHARTIIACIGPKTAETAAEFGLRVDVQPDTAAIGPLVDALAEHAARLRAEGALPPPRK
KSRRR

Sequences:

>Translated_565_residues
MTRGRKPRPGRIVFVGSGPGDPGLLTTRAAAVLANAALVFTDPDVPEPVVALIGTDLPPVSGPAPAEPVAGNGDAAGGGS
AQEHGRAASAVVSGGPDIRPALGDPADVAKTLTAEARSGVDVVRLVAGDPLTVDAVISEVNAVARTHLHIEIVPGLAASS
AVPTYAGLPLGSSHTVADVRIDPENTDWDALAAAPGPLILQATASHLAESARSLIDHQLAESTPCVVTAHGTTCQQRSVE
TTLQGLTDPAVLGATDPACSANGRDSQAGPLIVTIGKTVTSRAKLNWWESRALYGWTVLVPRTKDQAGEMSERLTSYGAL
PVEVPTIAVEPPRSPAQMERAVKGLVDGRFQWIVFTSTNAVRAVWEKFGEFGLDARAFSGVKIACVGESTADRVRAFGIS
PELVPSGEQSSLGLLDDFPPYDSVFDPVNRVLLPRADIATETLAEGLRERGWEIEDVTAYRTVRAAPPPATTREMIKTGG
FDAVCFTSSSTVRNLVGIAGKPHARTIIACIGPKTAETAAEFGLRVDVQPDTAAIGPLVDALAEHAARLRAEGALPPPRK
KSRRR
>Mature_564_residues
TRGRKPRPGRIVFVGSGPGDPGLLTTRAAAVLANAALVFTDPDVPEPVVALIGTDLPPVSGPAPAEPVAGNGDAAGGGSA
QEHGRAASAVVSGGPDIRPALGDPADVAKTLTAEARSGVDVVRLVAGDPLTVDAVISEVNAVARTHLHIEIVPGLAASSA
VPTYAGLPLGSSHTVADVRIDPENTDWDALAAAPGPLILQATASHLAESARSLIDHQLAESTPCVVTAHGTTCQQRSVET
TLQGLTDPAVLGATDPACSANGRDSQAGPLIVTIGKTVTSRAKLNWWESRALYGWTVLVPRTKDQAGEMSERLTSYGALP
VEVPTIAVEPPRSPAQMERAVKGLVDGRFQWIVFTSTNAVRAVWEKFGEFGLDARAFSGVKIACVGESTADRVRAFGISP
ELVPSGEQSSLGLLDDFPPYDSVFDPVNRVLLPRADIATETLAEGLRERGWEIEDVTAYRTVRAAPPPATTREMIKTGGF
DAVCFTSSSTVRNLVGIAGKPHARTIIACIGPKTAETAAEFGLRVDVQPDTAAIGPLVDALAEHAARLRAEGALPPPRKK
SRRR

Specific function: May catalyze sequential reactions to synthesize uroporphyrinogen III from hydroxymethylbilane (HMB) and then precorrin-2, which are intermediate compounds in both vitamin B12 and siroheme biosyntheses [H]

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=288, Percent_Identity=25.6944444444444, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR003754
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF02602 HEM4; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =4.2.1.75 [H]

Molecular weight: Translated: 58836; Mature: 58705

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRGRKPRPGRIVFVGSGPGDPGLLTTRAAAVLANAALVFTDPDVPEPVVALIGTDLPPV
CCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCEEEEECCCCCCHHHHHHCCCCCCC
SGPAPAEPVAGNGDAAGGGSAQEHGRAASAVVSGGPDIRPALGDPADVAKTLTAEARSGV
CCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
DVVRLVAGDPLTVDAVISEVNAVARTHLHIEIVPGLAASSAVPTYAGLPLGSSHTVADVR
EEEEEECCCCEEHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEE
IDPENTDWDALAAAPGPLILQATASHLAESARSLIDHQLAESTPCVVTAHGTTCQQRSVE
ECCCCCCCCCEECCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
TTLQGLTDPAVLGATDPACSANGRDSQAGPLIVTIGKTVTSRAKLNWWESRALYGWTVLV
HHHHCCCCCCEECCCCCCCCCCCCCCCCCCEEEEECCCHHCCHHCCHHHCCCEEEEEEEE
PRTKDQAGEMSERLTSYGALPVEVPTIAVEPPRSPAQMERAVKGLVDGRFQWIVFTSTNA
ECCCCHHHHHHHHHHHCCCCEEECCEEEECCCCCHHHHHHHHHHHHCCCEEEEEEECCHH
VRAVWEKFGEFGLDARAFSGVKIACVGESTADRVRAFGISPELVPSGEQSSLGLLDDFPP
HHHHHHHHHHCCCCCHHCCCEEEEEECCCHHHHHHEECCCCCCCCCCCCCCCCCCCCCCC
YDSVFDPVNRVLLPRADIATETLAEGLRERGWEIEDVTAYRTVRAAPPPATTREMIKTGG
CHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEHHEEEEECCCCCHHHHHHHHCCC
FDAVCFTSSSTVRNLVGIAGKPHARTIIACIGPKTAETAAEFGLRVDVQPDTAAIGPLVD
CCEEEEECCHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHCCCEEECCCCCHHHHHHHH
ALAEHAARLRAEGALPPPRKKSRRR
HHHHHHHHHHHCCCCCCCCHHCCCC
>Mature Secondary Structure 
TRGRKPRPGRIVFVGSGPGDPGLLTTRAAAVLANAALVFTDPDVPEPVVALIGTDLPPV
CCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCEEEEECCCCCCHHHHHHCCCCCCC
SGPAPAEPVAGNGDAAGGGSAQEHGRAASAVVSGGPDIRPALGDPADVAKTLTAEARSGV
CCCCCCCCCCCCCCCCCCCCHHHHCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
DVVRLVAGDPLTVDAVISEVNAVARTHLHIEIVPGLAASSAVPTYAGLPLGSSHTVADVR
EEEEEECCCCEEHHHHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEE
IDPENTDWDALAAAPGPLILQATASHLAESARSLIDHQLAESTPCVVTAHGTTCQQRSVE
ECCCCCCCCCEECCCCCEEEHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHH
TTLQGLTDPAVLGATDPACSANGRDSQAGPLIVTIGKTVTSRAKLNWWESRALYGWTVLV
HHHHCCCCCCEECCCCCCCCCCCCCCCCCCEEEEECCCHHCCHHCCHHHCCCEEEEEEEE
PRTKDQAGEMSERLTSYGALPVEVPTIAVEPPRSPAQMERAVKGLVDGRFQWIVFTSTNA
ECCCCHHHHHHHHHHHCCCCEEECCEEEECCCCCHHHHHHHHHHHHCCCEEEEEEECCHH
VRAVWEKFGEFGLDARAFSGVKIACVGESTADRVRAFGISPELVPSGEQSSLGLLDDFPP
HHHHHHHHHHCCCCCHHCCCEEEEEECCCHHHHHHEECCCCCCCCCCCCCCCCCCCCCCC
YDSVFDPVNRVLLPRADIATETLAEGLRERGWEIEDVTAYRTVRAAPPPATTREMIKTGG
CHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEHHEEEEECCCCCHHHHHHHHCCC
FDAVCFTSSSTVRNLVGIAGKPHARTIIACIGPKTAETAAEFGLRVDVQPDTAAIGPLVD
CCEEEEECCHHHHHHHHCCCCCCCCEEEEEECCCHHHHHHHCCCEEECCCCCHHHHHHHH
ALAEHAARLRAEGALPPPRKKSRRR
HHHHHHHHHHHCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7665501 [H]