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The map label for this gene is murB

Identifier: 148660249

GI number: 148660249

Start: 571849

End: 572958

Strand: Direct

Name: murB

Synonym: MRA_0489

Alternate gene names: 148660249

Gene position: 571849-572958 (Clockwise)

Preceding gene: 148660245

Following gene: 148660250

Centisome position: 12.94

GC content: 66.31

Gene sequence:

>1110_bases
ATGAAACGGAGCGGTGTCGGTTCGCTCTTTGCCGGTGCGCATATTGCCGAGGCGGTCCCGTTGGCGCCGCTGACCACTTT
GCGTGTGGGCCCGATCGCCCGACGTGTCATCACTTGCACCAGCGCCGAACAGGTGGTGGCTGCGCTGCGGCACCTGGATT
CGGCGGCCAAGACCGGAGCTGACCGCCCGCTGGTGTTTGCTGGTGGCTCCAATTTGGTGATCGCCGAGAACCTGACCGAC
CTGACCGTGGTGCGGTTGGCCAATAGCGGCATCACCATCGACGGTAACTTGGTGCGGGCCGAGGCCGGTGCGGTCTTCGA
TGACGTGGTGGTTAGGGCCATCGAACAGGGTCTGGGCGGACTGGAATGCCTGTCTGGCATCCCAGGATCGGCCGGGGCGA
CACCCGTGCAGAACGTGGGGGCGTATGGCGCGGAGGTGTCTGACACCATCACTCGGGTTCGGCTTTTGGATCGGTGCACG
GGTGAGGTGCGTTGGGTATCCGCGCGCGACCTGCGCTTCGGCTATCGCACGAGCGTGCTCAAACACGCTGATGGGCTTGC
GGTGCCCACCGTGGTCTTGGAGGTGGAGTTTGCGCTGGATCCGTCGGGCCGCAGCGCACCGCTGCGCTACGGCGAGCTGA
TCGCCGCGCTGAATGCGACCAGCGGCGAGCGCGCCGACCCGCAAGCGGTCCGCGAAGCGGTGCTGGCCCTGCGGGCACGC
AAGGGCATGGTGCTGGACCCGACCGACCATGACACCTGGAGCGTGGGATCGTTCTTCACAAACCCGGTGGTCACCCAGGA
TGTTTACGAACGGCTGGCCGGTGACGCGGCCACCAGAAAGGACGGTCCGGTCCCGCACTATCCCGCGCCCGACGGCGTCA
AGCTGGCCGCCGGCTGGCTGGTGGAACGGGCCGGCTTCGGCAAGGGCTATCCGGATGCCGGCGCCGCCCCATGCCGGCTT
TCCACCAAACATGCGCTGGCGCTGACAAATCGTGGCGGGGCCACCGCCGAAGATGTGGTGACGCTGGCGCGCGCCGTGCG
CGATGGGGTCCATGATGTGTTTGGTATCACACTAAAACCCGAACCCGTGCTGATCGGCTGCATGTTGTAG

Upstream 100 bases:

>100_bases
GCCTCGTAGAAAGCCCGATGAACCTCCTCGACGCTGCCCTCGTAGTCGGCCGACATGTCGAATGAACGCGGCATAGCAGG
TCAGGCTACCCTTACGGGCC

Downstream 100 bases:

>100_bases
CTGCGTTTTCGCGGCGGGGCGGCGTGGCGCGCATTGCTTAGGGCTGGTTGCCAGGCGTTCTGTGGTCATTCGTGTGCTGT
TTCGCCCGGTATCTTTGATA

Product: UDP-N-acetylenolpyruvoylglucosamine reductase

Products: NA

Alternate protein names: UDP-N-acetylmuramate dehydrogenase

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MKRSGVGSLFAGAHIAEAVPLAPLTTLRVGPIARRVITCTSAEQVVAALRHLDSAAKTGADRPLVFAGGSNLVIAENLTD
LTVVRLANSGITIDGNLVRAEAGAVFDDVVVRAIEQGLGGLECLSGIPGSAGATPVQNVGAYGAEVSDTITRVRLLDRCT
GEVRWVSARDLRFGYRTSVLKHADGLAVPTVVLEVEFALDPSGRSAPLRYGELIAALNATSGERADPQAVREAVLALRAR
KGMVLDPTDHDTWSVGSFFTNPVVTQDVYERLAGDAATRKDGPVPHYPAPDGVKLAAGWLVERAGFGKGYPDAGAAPCRL
STKHALALTNRGGATAEDVVTLARAVRDGVHDVFGITLKPEPVLIGCML

Sequences:

>Translated_369_residues
MKRSGVGSLFAGAHIAEAVPLAPLTTLRVGPIARRVITCTSAEQVVAALRHLDSAAKTGADRPLVFAGGSNLVIAENLTD
LTVVRLANSGITIDGNLVRAEAGAVFDDVVVRAIEQGLGGLECLSGIPGSAGATPVQNVGAYGAEVSDTITRVRLLDRCT
GEVRWVSARDLRFGYRTSVLKHADGLAVPTVVLEVEFALDPSGRSAPLRYGELIAALNATSGERADPQAVREAVLALRAR
KGMVLDPTDHDTWSVGSFFTNPVVTQDVYERLAGDAATRKDGPVPHYPAPDGVKLAAGWLVERAGFGKGYPDAGAAPCRL
STKHALALTNRGGATAEDVVTLARAVRDGVHDVFGITLKPEPVLIGCML
>Mature_369_residues
MKRSGVGSLFAGAHIAEAVPLAPLTTLRVGPIARRVITCTSAEQVVAALRHLDSAAKTGADRPLVFAGGSNLVIAENLTD
LTVVRLANSGITIDGNLVRAEAGAVFDDVVVRAIEQGLGGLECLSGIPGSAGATPVQNVGAYGAEVSDTITRVRLLDRCT
GEVRWVSARDLRFGYRTSVLKHADGLAVPTVVLEVEFALDPSGRSAPLRYGELIAALNATSGERADPQAVREAVLALRAR
KGMVLDPTDHDTWSVGSFFTNPVVTQDVYERLAGDAATRKDGPVPHYPAPDGVKLAAGWLVERAGFGKGYPDAGAAPCRL
STKHALALTNRGGATAEDVVTLARAVRDGVHDVFGITLKPEPVLIGCML

Specific function: Cell wall formation

COG id: COG0812

COG function: function code M; UDP-N-acetylmuramate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain

Homologues:

Organism=Escherichia coli, GI1790407, Length=354, Percent_Identity=33.3333333333333, Blast_Score=150, Evalue=9e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURB_MYCBO (P65461)

Other databases:

- EMBL:   BX248335
- RefSeq:   NP_854155.1
- ProteinModelPortal:   P65461
- SMR:   P65461
- EnsemblBacteria:   EBMYCT00000016863
- GeneID:   1091531
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb0492
- GeneTree:   EBGT00050000015845
- HOGENOM:   HBG686573
- OMA:   GAAPMQN
- ProtClustDB:   PRK13903
- BioCyc:   MBOV233413:MB0492-MONOMER
- BRENDA:   1.1.1.158
- GO:   GO:0005737
- HAMAP:   MF_00037
- InterPro:   IPR016169
- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR003170
- InterPro:   IPR011601
- InterPro:   IPR006094
- Gene3D:   G3DSA:3.30.465.10
- Gene3D:   G3DSA:3.30.43.10
- Gene3D:   G3DSA:3.90.78.10
- PANTHER:   PTHR21071
- TIGRFAMs:   TIGR00179

Pfam domain/function: PF01565 FAD_binding_4; PF02873 MurB_C; SSF56176 FAD-binding_2; SSF56194 MurB_C

EC number: =1.1.1.158

Molecular weight: Translated: 38521; Mature: 38521

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS51387 FAD_PCMH

Important sites: ACT_SITE 176-176 ACT_SITE 257-257 ACT_SITE 361-361

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRSGVGSLFAGAHIAEAVPLAPLTTLRVGPIARRVITCTSAEQVVAALRHLDSAAKTGA
CCCCCCCHHHHHHHHHHHCCCCCHHHHHHCHHHHHHEEECCHHHHHHHHHHHHHHHHCCC
DRPLVFAGGSNLVIAENLTDLTVVRLANSGITIDGNLVRAEAGAVFDDVVVRAIEQGLGG
CCCEEEECCCCEEEECCCCCEEEEEEECCCEEECCCEEEECCCCHHHHHHHHHHHHCCCH
LECLSGIPGSAGATPVQNVGAYGAEVSDTITRVRLLDRCTGEVRWVSARDLRFGYRTSVL
HHHHCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHCCCCHHHHHHH
KHADGLAVPTVVLEVEFALDPSGRSAPLRYGELIAALNATSGERADPQAVREAVLALRAR
HHCCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHC
KGMVLDPTDHDTWSVGSFFTNPVVTQDVYERLAGDAATRKDGPVPHYPAPDGVKLAAGWL
CCCEECCCCCCCEECCCHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEHHHHH
VERAGFGKGYPDAGAAPCRLSTKHALALTNRGGATAEDVVTLARAVRDGVHDVFGITLKP
HHHCCCCCCCCCCCCCCCEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHEEEEECC
EPVLIGCML
CCEEEEECC
>Mature Secondary Structure
MKRSGVGSLFAGAHIAEAVPLAPLTTLRVGPIARRVITCTSAEQVVAALRHLDSAAKTGA
CCCCCCCHHHHHHHHHHHCCCCCHHHHHHCHHHHHHEEECCHHHHHHHHHHHHHHHHCCC
DRPLVFAGGSNLVIAENLTDLTVVRLANSGITIDGNLVRAEAGAVFDDVVVRAIEQGLGG
CCCEEEECCCCEEEECCCCCEEEEEEECCCEEECCCEEEECCCCHHHHHHHHHHHHCCCH
LECLSGIPGSAGATPVQNVGAYGAEVSDTITRVRLLDRCTGEVRWVSARDLRFGYRTSVL
HHHHCCCCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEEEHHCCCCHHHHHHH
KHADGLAVPTVVLEVEFALDPSGRSAPLRYGELIAALNATSGERADPQAVREAVLALRAR
HHCCCCCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHC
KGMVLDPTDHDTWSVGSFFTNPVVTQDVYERLAGDAATRKDGPVPHYPAPDGVKLAAGWL
CCCEECCCCCCCEECCCHHCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEHHHHH
VERAGFGKGYPDAGAAPCRLSTKHALALTNRGGATAEDVVTLARAVRDGVHDVFGITLKP
HHHCCCCCCCCCCCCCCCEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHEEEEECC
EPVLIGCML
CCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972