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The map label for this gene is rskA [H]

Identifier: 148660209

GI number: 148660209

Start: 534401

End: 535099

Strand: Reverse

Name: rskA [H]

Synonym: MRA_0449

Alternate gene names: 148660209

Gene position: 535099-534401 (Counterclockwise)

Preceding gene: 148660210

Following gene: 148660207

Centisome position: 12.11

GC content: 68.53

Gene sequence:

>699_bases
ATGACTGAACATACCGATTTTGAGCTGCTCGAGCTGGCTACCCCGTATGCCCTGAACGCTGTGTCCGACGATGAACGAGC
CGACATCGACCGACGGGTGGCCGCCGCGCCCTCGCCGGTAGCCGCGGCTTTCAACGACGAAGTTCGAGCCGTCCGCGAGA
CGATGGCCGTCGTCTCGGCGGCCACCACCGCCGAGCCGCCAGCACATCTGCGGACGGCCATCCTGGATGCGACCAAGCCC
GAGGTTAGGCGCCAATCACGCTGGCGTACGGCAGCCTTTGCGTCCGCAGCGGCGATCGCTGTGGGGCTGGGGGCGTTCGG
CCTGGGGGTGCTGACCCGGCCATCGCCACCCCCCACCGTGGCCGAGCAAGTTCTGACGGCGCCGGACGTACGGACGGTCT
CTCGCCCACTGGGCGCCGGGACGGCCACGGTGGTGTTCTCCCGCGACCGCAACACAGGTCTGCTGGTGATGAACAATGTG
GCGCCACCATCCCGGGGAACCGTGTATCAGATGTGGCTGCTGGGAGGGGCCAAAGGGCCGAGGTCGGCGGGGACGATGGG
CACCGCGGCGGTGACGCCCTCGACGACGGCGACGCTGACCGATCTCGGAGCCTCGACCGCACTGGCATTCACCGTCGAAC
CCGGCACCGGATCACCACAGCCCACCGGTACCATCTTGGCCGAGCTGCCGCTCGGGTGA

Upstream 100 bases:

>100_bases
AAATCCCGCATGCGCGACGCCCTGCGCAGCCTGCGCAACTGCCTGGACGTGTCATGACTGCGCCGGCCGAGCGAAGCGAT
GCGTTGGGAGAGCGGCGCTC

Downstream 100 bases:

>100_bases
GCCGAGGACGCACACCCCGGCCCCTCACCGCAGTCTCGGCGCGACAATGGTGCCCAGCATCGCGACGAACACGCCGGGTA
CCACTAGGGCGGCGCTGCCG

Product: hypothetical protein

Products: NA

Alternate protein names: Regulator of sigK [H]

Number of amino acids: Translated: 232; Mature: 231

Protein sequence:

>232_residues
MTEHTDFELLELATPYALNAVSDDERADIDRRVAAAPSPVAAAFNDEVRAVRETMAVVSAATTAEPPAHLRTAILDATKP
EVRRQSRWRTAAFASAAAIAVGLGAFGLGVLTRPSPPPTVAEQVLTAPDVRTVSRPLGAGTATVVFSRDRNTGLLVMNNV
APPSRGTVYQMWLLGGAKGPRSAGTMGTAAVTPSTTATLTDLGASTALAFTVEPGTGSPQPTGTILAELPLG

Sequences:

>Translated_232_residues
MTEHTDFELLELATPYALNAVSDDERADIDRRVAAAPSPVAAAFNDEVRAVRETMAVVSAATTAEPPAHLRTAILDATKP
EVRRQSRWRTAAFASAAAIAVGLGAFGLGVLTRPSPPPTVAEQVLTAPDVRTVSRPLGAGTATVVFSRDRNTGLLVMNNV
APPSRGTVYQMWLLGGAKGPRSAGTMGTAAVTPSTTATLTDLGASTALAFTVEPGTGSPQPTGTILAELPLG
>Mature_231_residues
TEHTDFELLELATPYALNAVSDDERADIDRRVAAAPSPVAAAFNDEVRAVRETMAVVSAATTAEPPAHLRTAILDATKPE
VRRQSRWRTAAFASAAAIAVGLGAFGLGVLTRPSPPPTVAEQVLTAPDVRTVSRPLGAGTATVVFSRDRNTGLLVMNNVA
PPSRGTVYQMWLLGGAKGPRSAGTMGTAAVTPSTTATLTDLGASTALAFTVEPGTGSPQPTGTILAELPLG

Specific function: Represses sigK, the extracytoplasmic function (ECF) sigma factor K, by binding to it and inhibiting its activity. However, in M.bovis it is probably dysfunctional, due to at least one of the two natural occurring polymorphisms in its encoding gene, when c

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the anti-sigma-K factor family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018764 [H]

Pfam domain/function: PF10099 DUF2337 [H]

EC number: NA

Molecular weight: Translated: 23883; Mature: 23752

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEHTDFELLELATPYALNAVSDDERADIDRRVAAAPSPVAAAFNDEVRAVRETMAVVSA
CCCCCCCHHHHHCCCEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ATTAEPPAHLRTAILDATKPEVRRQSRWRTAAFASAAAIAVGLGAFGLGVLTRPSPPPTV
HCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCCCHHH
AEQVLTAPDVRTVSRPLGAGTATVVFSRDRNTGLLVMNNVAPPSRGTVYQMWLLGGAKGP
HHHHHCCCCHHHHHCCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCEEEEEEECCCCCC
RSAGTMGTAAVTPSTTATLTDLGASTALAFTVEPGTGSPQPTGTILAELPLG
CCCCCCCCEEECCCCCCHHHHCCCCEEEEEEEECCCCCCCCCCEEEEECCCC
>Mature Secondary Structure 
TEHTDFELLELATPYALNAVSDDERADIDRRVAAAPSPVAAAFNDEVRAVRETMAVVSA
CCCCCCHHHHHCCCEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ATTAEPPAHLRTAILDATKPEVRRQSRWRTAAFASAAAIAVGLGAFGLGVLTRPSPPPTV
HCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEECCCCCCHHH
AEQVLTAPDVRTVSRPLGAGTATVVFSRDRNTGLLVMNNVAPPSRGTVYQMWLLGGAKGP
HHHHHCCCCHHHHHCCCCCCEEEEEEECCCCCCEEEECCCCCCCCCCEEEEEEECCCCCC
RSAGTMGTAAVTPSTTATLTDLGASTALAFTVEPGTGSPQPTGTILAELPLG
CCCCCCCCEEECCCCCCHHHHCCCCEEEEEEEECCCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]