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The map label for this gene is ackA

Identifier: 148660174

GI number: 148660174

Start: 495161

End: 496318

Strand: Direct

Name: ackA

Synonym: MRA_0415

Alternate gene names: 148660174

Gene position: 495161-496318 (Clockwise)

Preceding gene: 148660173

Following gene: 148660178

Centisome position: 11.2

GC content: 64.68

Gene sequence:

>1158_bases
ATGAGTAGCACCGTGCTGGTGATCAATTCCGGCTCGTCGTCGCTGAAGTTCCAGCTCGTCGAGCCGGTCGCCGGCATGTC
ACGTGCCGCCGGGATTGTCGAGCGGATCGGCGAGCGGTCATCCCCGGTTGCCGATCACGCCCAGGCGCTGCATCGCGCAT
TCAAGATGTTGGCCGAGGACGGAATTGACCTGCAGACCTGCGGGCTGGTGGCGGTCGGACACCGGGTGGTCCACGGCGGC
ACGGAGTTTCACCAGCCGACGCTGCTGGATGACACGGTGATCGGCAAGCTTGAGGAGCTGTCGGCGCTGGCCCCGTTGCA
CAACCCGCCGGCGGTACTGGGCATCAAGGTGGCACGCAGATTGCTGGCCAATGTCGCGCACGTCGCGGTGTTCGATACGG
CCTTTTTCCATGACTTGCCCCCGGCGGCCGCGACCTATGCCATCGACCGCGACGTCGCCGACAGATGGCATATCCGCCGC
TACGGATTTCATGGCACTTCACACCAATACGTCAGCGAGCGGGCCGCCGCCTTCCTGGGCCGCCCGCTCGACGGTTTGAA
TCAGATTGTGCTGCATCTGGGTAACGGTGCCTCCGCCTCGGCGATTGCCCGCGGCCGGCCGGTGGAAACGTCGATGGGCC
TGACACCGCTTGAGGGCTTGGTGATGGGCACCCGCAGTGGCGACCTGGACCCGGGCGTCATCAGCTACTTGTGGCGCACC
GCGAGGATGGGTGTCGAGGACATCGAATCGATGCTCAACCATCGGTCCGGGATGTTGGGGTTGGCGGGGGAGCGGGATTT
TCGCCGTCTACGACTAGTGATCGAAACCGGGGACAGGTCAGCACAATTGGCGTATGAGGTGTTCATCCACCGGTTGCGCA
AGTACCTTGGTGCCTATCTGGCGGTGTTGGGCCACACCGATGTGGTGAGCTTTACCGCCGGGATCGGCGAAAACGATGCG
GCGGTGCGGCGGGACGCGTTGGCTGGCCTTCAGGGGCTAGGTATCGCACTCGACCAAGACCGCAACCTGGGCCCGGGGCA
CGGCGCCCGGCGGATTTCGTCAGACGATTCACCGATCGCCGTGCTGGTGGTTCCCACGAATGAAGAACTGGCCATCGCCC
GCGATTGCCTGAGGGTGCTGGGCGGACGCCGAGCGTGA

Upstream 100 bases:

>100_bases
CAGGGCTTACGCAAGCCGGTGAACGACCTATCTCGGGGTGCACTGGTCGACGACATAGTCAACACCGTGGCCATCACGGC
GATTCAGGCGCAGGGCGTCC

Downstream 100 bases:

>100_bases
ATCATACGACAGCCCGCCGGCGTGTCGCGTCGTGCGATTCACACTCGGGCGGCTTAGAACGTGCTGGTGGGCCGGACCTT
GTTGGCCATGTCCACCAGCG

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MSSTVLVINSGSSSLKFQLVEPVAGMSRAAGIVERIGERSSPVADHAQALHRAFKMLAEDGIDLQTCGLVAVGHRVVHGG
TEFHQPTLLDDTVIGKLEELSALAPLHNPPAVLGIKVARRLLANVAHVAVFDTAFFHDLPPAAATYAIDRDVADRWHIRR
YGFHGTSHQYVSERAAAFLGRPLDGLNQIVLHLGNGASASAIARGRPVETSMGLTPLEGLVMGTRSGDLDPGVISYLWRT
ARMGVEDIESMLNHRSGMLGLAGERDFRRLRLVIETGDRSAQLAYEVFIHRLRKYLGAYLAVLGHTDVVSFTAGIGENDA
AVRRDALAGLQGLGIALDQDRNLGPGHGARRISSDDSPIAVLVVPTNEELAIARDCLRVLGGRRA

Sequences:

>Translated_385_residues
MSSTVLVINSGSSSLKFQLVEPVAGMSRAAGIVERIGERSSPVADHAQALHRAFKMLAEDGIDLQTCGLVAVGHRVVHGG
TEFHQPTLLDDTVIGKLEELSALAPLHNPPAVLGIKVARRLLANVAHVAVFDTAFFHDLPPAAATYAIDRDVADRWHIRR
YGFHGTSHQYVSERAAAFLGRPLDGLNQIVLHLGNGASASAIARGRPVETSMGLTPLEGLVMGTRSGDLDPGVISYLWRT
ARMGVEDIESMLNHRSGMLGLAGERDFRRLRLVIETGDRSAQLAYEVFIHRLRKYLGAYLAVLGHTDVVSFTAGIGENDA
AVRRDALAGLQGLGIALDQDRNLGPGHGARRISSDDSPIAVLVVPTNEELAIARDCLRVLGGRRA
>Mature_384_residues
SSTVLVINSGSSSLKFQLVEPVAGMSRAAGIVERIGERSSPVADHAQALHRAFKMLAEDGIDLQTCGLVAVGHRVVHGGT
EFHQPTLLDDTVIGKLEELSALAPLHNPPAVLGIKVARRLLANVAHVAVFDTAFFHDLPPAAATYAIDRDVADRWHIRRY
GFHGTSHQYVSERAAAFLGRPLDGLNQIVLHLGNGASASAIARGRPVETSMGLTPLEGLVMGTRSGDLDPGVISYLWRTA
RMGVEDIESMLNHRSGMLGLAGERDFRRLRLVIETGDRSAQLAYEVFIHRLRKYLGAYLAVLGHTDVVSFTAGIGENDAA
VRRDALAGLQGLGIALDQDRNLGPGHGARRISSDDSPIAVLVVPTNEELAIARDCLRVLGGRRA

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family

Homologues:

Organism=Escherichia coli, GI1788633, Length=398, Percent_Identity=41.9597989949749, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI145693184, Length=391, Percent_Identity=38.8746803069054, Blast_Score=258, Evalue=6e-70,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ACKA_MYCBO (P63410)

Other databases:

- EMBL:   BX248335
- RefSeq:   NP_854080.1
- ProteinModelPortal:   P63410
- SMR:   P63410
- EnsemblBacteria:   EBMYCT00000015616
- GeneID:   1091412
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb0417
- GeneTree:   EBGT00050000016195
- HOGENOM:   HBG285863
- OMA:   GGELYSE
- ProtClustDB:   PRK00180
- BioCyc:   MBOV233413:MB0417-MONOMER
- BRENDA:   2.7.2.1
- GO:   GO:0005737
- HAMAP:   MF_00020
- InterPro:   IPR000890
- InterPro:   IPR004372
- PANTHER:   PTHR21060
- PIRSF:   PIRSF000722
- PRINTS:   PR00471
- TIGRFAMs:   TIGR00016

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.1

Molecular weight: Translated: 41319; Mature: 41188

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2; PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSTVLVINSGSSSLKFQLVEPVAGMSRAAGIVERIGERSSPVADHAQALHRAFKMLAED
CCCEEEEEECCCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHC
GIDLQTCGLVAVGHRVVHGGTEFHQPTLLDDTVIGKLEELSALAPLHNPPAVLGIKVARR
CCCHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LLANVAHVAVFDTAFFHDLPPAAATYAIDRDVADRWHIRRYGFHGTSHQYVSERAAAFLG
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHC
RPLDGLNQIVLHLGNGASASAIARGRPVETSMGLTPLEGLVMGTRSGDLDPGVISYLWRT
CCHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCHHHEEEECCCCCCCHHHHHHHHHH
ARMGVEDIESMLNHRSGMLGLAGERDFRRLRLVIETGDRSAQLAYEVFIHRLRKYLGAYL
HHCCHHHHHHHHHHCCCCEECCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
AVLGHTDVVSFTAGIGENDAAVRRDALAGLQGLGIALDQDRNLGPGHGARRISSDDSPIA
HHHCCCHHHHHCCCCCCCHHHHHHHHHHCHHHCCEEEECCCCCCCCCCCCCCCCCCCCEE
VLVVPTNEELAIARDCLRVLGGRRA
EEEEECCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SSTVLVINSGSSSLKFQLVEPVAGMSRAAGIVERIGERSSPVADHAQALHRAFKMLAED
CCEEEEEECCCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHC
GIDLQTCGLVAVGHRVVHGGTEFHQPTLLDDTVIGKLEELSALAPLHNPPAVLGIKVARR
CCCHHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LLANVAHVAVFDTAFFHDLPPAAATYAIDRDVADRWHIRRYGFHGTSHQYVSERAAAFLG
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHC
RPLDGLNQIVLHLGNGASASAIARGRPVETSMGLTPLEGLVMGTRSGDLDPGVISYLWRT
CCHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCHHHEEEECCCCCCCHHHHHHHHHH
ARMGVEDIESMLNHRSGMLGLAGERDFRRLRLVIETGDRSAQLAYEVFIHRLRKYLGAYL
HHCCHHHHHHHHHHCCCCEECCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHH
AVLGHTDVVSFTAGIGENDAAVRRDALAGLQGLGIALDQDRNLGPGHGARRISSDDSPIA
HHHCCCHHHHHCCCCCCCHHHHHHHHHHCHHHCCEEEECCCCCCCCCCCCCCCCCCCCEE
VLVVPTNEELAIARDCLRVLGGRRA
EEEEECCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972