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The map label for this gene is ansP2

Identifier: 148660112

GI number: 148660112

Start: 416864

End: 418327

Strand: Reverse

Name: ansP2

Synonym: MRA_0355

Alternate gene names: 148660112

Gene position: 418327-416864 (Counterclockwise)

Preceding gene: 148660120

Following gene: 148660110

Centisome position: 9.46

GC content: 62.57

Gene sequence:

>1464_bases
ATGCCACCGCTAGACATCACCGACGAGCGGCTCACGCGCGAAGACACGGGCTATCACAAAGGCCTCCATAGCCGGCAACT
GCAAATGATCGCTCTCGGTGGTGCCATCGGTACCGGCCTTTTTCTCGGCGCCGGCGGCCGCCTTGCATCGGCGGGGCCCG
GTTTGTTCCTGGTCTATGGGATCTGCGGCATCTTCGTGTTCCTGATCCTGCGCGCGCTCGGCGAACTGGTGCTGCACCGT
CCGTCCTCGGGGTCATTCGTGTCCTACGCCCGCGAGTTCTACGGGGAAAAGGTGGCCTTCGTCGCCGGGTGGATGTACTT
CCTGAACTGGGCGATGACGGGGATCGTCGATACCACCGCGATCGCGCACTATTGCCATTACTGGCGGGCGTTTCAACCCA
TCCCCCAGTGGACGCTGGCGTTGATCGCGCTGCTGGTCGTGTTGTCGATGAACCTGATCTCGGTCAGGCTCTTCGGCGAG
CTGGAGTTCTGGGCCTCATTGATCAAGGTGATCGCGCTGGTTACGTTTCTGATCGTCGGTACGGTCTTTCTCGCTGGGCG
CTACAAGATCGACGGCCAGGAGACCGGGGTCTCCCTGTGGAGCAGCCATGGAGGCATCGTGCCAACCGGTCTGCTGCCCA
TAGTGCTGGTCACCTCGGGGGTGGTATTCGCCTACGCCGCAATCGAACTGGTGGGCATCGCGGCCGGGGAAACAGCCGAA
CCGGCAAAGATCATGCCGCGCGCGATCAACTCTGTGGTGTTGCGCATCGCGTGCTTCTACGTCGGGTCGACTGTCCTGCT
GGCCCTGCTGCTGCCCTATACCGCCTACAAAGAACATGTCAGCCCGTTCGTGACCTTCTTTTCCAAGATCGGTATCGACG
CGGCGGGCAGCGTGATGAACCTCGTCGTGCTCACCGCCGCGCTGTCGAGCCTCAACGCCGGACTGTATTCCACCGGACGG
ATCCTGCGATCAATGGCGATCAACGGCAGCGGCCCGAGGTTCACCGCACCAATGTCGAAGACCGGCGTGCCGTACGGCGG
GATCCTGCTCACCGCCGGTATCGGCCTGTTGGGCATCATTCTCAACGCCATCAAACCGAGCCAAGCCTTCGAGATCGTGC
TCCACATCGCCGCGACGGGAGTCATCGCGGCCTGGGCGACGATCGTGGCTTGCCAGCTGCGGCTCCACCGGATGGCGAAC
GCCGGGCAGCTGCAGCGGCCGAAATTCCGGATGCCGCTTTCACCGTTCAGCGGCTATCTGACGCTGGCTTTCCTGGCCGG
CGTGCTGATCCTGATGTACTTCGACGAACAACACGGTCCCTGGATGATCGCCGCAACGGTGATCGGTGTACCGGCCCTCA
TCGGTGGCTGGTATTTGGTCCGCAATCGTGTGACGGCCGTCGCGCACCACGCCATCGACCACACCAAGAGTGTCGCGGTC
GTCCATTCGGCGGATCCAATCTGA

Upstream 100 bases:

>100_bases
AAGCGGCACCTCGCAGCCGCGTGGGATAGCCTGACAGCCAGTCCGTTGGGTGTGCCCCATCTCACGCGCGTCCCAAGAAG
CCACCATCCCAGGGAGCTCA

Downstream 100 bases:

>100_bases
GGTCCACGTCGACATCGCGACCACTAGCCAAGTCGCCGCACTCAACAGCCTCGACGTCGGGCACTGTGCGCAGGTATGCG
GCCGCGCCCTGCCAGACCGC

Product: L-asparagine permease

Products: Proton [Cytoplasm]; L-asparagine [Cytoplasm] [C]

Alternate protein names: L-asparagine transport protein 2

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MPPLDITDERLTREDTGYHKGLHSRQLQMIALGGAIGTGLFLGAGGRLASAGPGLFLVYGICGIFVFLILRALGELVLHR
PSSGSFVSYAREFYGEKVAFVAGWMYFLNWAMTGIVDTTAIAHYCHYWRAFQPIPQWTLALIALLVVLSMNLISVRLFGE
LEFWASLIKVIALVTFLIVGTVFLAGRYKIDGQETGVSLWSSHGGIVPTGLLPIVLVTSGVVFAYAAIELVGIAAGETAE
PAKIMPRAINSVVLRIACFYVGSTVLLALLLPYTAYKEHVSPFVTFFSKIGIDAAGSVMNLVVLTAALSSLNAGLYSTGR
ILRSMAINGSGPRFTAPMSKTGVPYGGILLTAGIGLLGIILNAIKPSQAFEIVLHIAATGVIAAWATIVACQLRLHRMAN
AGQLQRPKFRMPLSPFSGYLTLAFLAGVLILMYFDEQHGPWMIAATVIGVPALIGGWYLVRNRVTAVAHHAIDHTKSVAV
VHSADPI

Sequences:

>Translated_487_residues
MPPLDITDERLTREDTGYHKGLHSRQLQMIALGGAIGTGLFLGAGGRLASAGPGLFLVYGICGIFVFLILRALGELVLHR
PSSGSFVSYAREFYGEKVAFVAGWMYFLNWAMTGIVDTTAIAHYCHYWRAFQPIPQWTLALIALLVVLSMNLISVRLFGE
LEFWASLIKVIALVTFLIVGTVFLAGRYKIDGQETGVSLWSSHGGIVPTGLLPIVLVTSGVVFAYAAIELVGIAAGETAE
PAKIMPRAINSVVLRIACFYVGSTVLLALLLPYTAYKEHVSPFVTFFSKIGIDAAGSVMNLVVLTAALSSLNAGLYSTGR
ILRSMAINGSGPRFTAPMSKTGVPYGGILLTAGIGLLGIILNAIKPSQAFEIVLHIAATGVIAAWATIVACQLRLHRMAN
AGQLQRPKFRMPLSPFSGYLTLAFLAGVLILMYFDEQHGPWMIAATVIGVPALIGGWYLVRNRVTAVAHHAIDHTKSVAV
VHSADPI
>Mature_486_residues
PPLDITDERLTREDTGYHKGLHSRQLQMIALGGAIGTGLFLGAGGRLASAGPGLFLVYGICGIFVFLILRALGELVLHRP
SSGSFVSYAREFYGEKVAFVAGWMYFLNWAMTGIVDTTAIAHYCHYWRAFQPIPQWTLALIALLVVLSMNLISVRLFGEL
EFWASLIKVIALVTFLIVGTVFLAGRYKIDGQETGVSLWSSHGGIVPTGLLPIVLVTSGVVFAYAAIELVGIAAGETAEP
AKIMPRAINSVVLRIACFYVGSTVLLALLLPYTAYKEHVSPFVTFFSKIGIDAAGSVMNLVVLTAALSSLNAGLYSTGRI
LRSMAINGSGPRFTAPMSKTGVPYGGILLTAGIGLLGIILNAIKPSQAFEIVLHIAATGVIAAWATIVACQLRLHRMANA
GQLQRPKFRMPLSPFSGYLTLAFLAGVLILMYFDEQHGPWMIAATVIGVPALIGGWYLVRNRVTAVAHHAIDHTKSVAVV
HSADPI

Specific function: Unknown

COG id: COG1113

COG function: function code E; Gamma-aminobutyrate permease and related permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily. Amino acid transporter (AAT) (TC 2.A.3.1) family

Homologues:

Organism=Homo sapiens, GI258645169, Length=436, Percent_Identity=25.2293577981651, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI87081915, Length=470, Percent_Identity=58.5106382978723, Blast_Score=523, Evalue=1e-150,
Organism=Escherichia coli, GI1786789, Length=427, Percent_Identity=36.768149882904, Blast_Score=304, Evalue=8e-84,
Organism=Escherichia coli, GI1786302, Length=418, Percent_Identity=37.5598086124402, Blast_Score=287, Evalue=1e-78,
Organism=Escherichia coli, GI1790653, Length=439, Percent_Identity=35.3075170842825, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI48994972, Length=421, Percent_Identity=39.1923990498812, Blast_Score=277, Evalue=1e-75,
Organism=Escherichia coli, GI1786602, Length=421, Percent_Identity=36.104513064133, Blast_Score=259, Evalue=4e-70,
Organism=Escherichia coli, GI1788480, Length=468, Percent_Identity=34.6153846153846, Blast_Score=248, Evalue=8e-67,
Organism=Escherichia coli, GI87081708, Length=411, Percent_Identity=33.5766423357664, Blast_Score=232, Evalue=3e-62,
Organism=Escherichia coli, GI1789017, Length=416, Percent_Identity=31.4903846153846, Blast_Score=221, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6324061, Length=451, Percent_Identity=31.7073170731707, Blast_Score=218, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6320772, Length=452, Percent_Identity=30.5309734513274, Blast_Score=200, Evalue=4e-52,
Organism=Saccharomyces cerevisiae, GI6324059, Length=460, Percent_Identity=31.7391304347826, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6322892, Length=430, Percent_Identity=30.6976744186047, Blast_Score=177, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6324990, Length=474, Percent_Identity=27.6371308016878, Blast_Score=168, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6321053, Length=441, Percent_Identity=26.984126984127, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6324553, Length=399, Percent_Identity=31.5789473684211, Blast_Score=161, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6321629, Length=409, Percent_Identity=28.6063569682152, Blast_Score=147, Evalue=5e-36,
Organism=Saccharomyces cerevisiae, GI6324924, Length=448, Percent_Identity=27.9017857142857, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6320717, Length=403, Percent_Identity=26.302729528536, Blast_Score=143, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6322967, Length=417, Percent_Identity=28.537170263789, Blast_Score=142, Evalue=9e-35,
Organism=Saccharomyces cerevisiae, GI6319543, Length=415, Percent_Identity=27.710843373494, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6319824, Length=400, Percent_Identity=26, Blast_Score=139, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6324981, Length=420, Percent_Identity=29.5238095238095, Blast_Score=136, Evalue=7e-33,
Organism=Saccharomyces cerevisiae, GI6320251, Length=408, Percent_Identity=23.7745098039216, Blast_Score=131, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6319542, Length=411, Percent_Identity=24.0875912408759, Blast_Score=118, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6319608, Length=457, Percent_Identity=24.507658643326, Blast_Score=113, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6320364, Length=507, Percent_Identity=23.2741617357002, Blast_Score=90, Evalue=8e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ANSP2_MYCBO (P0A4W1)

Other databases:

- EMBL:   BX248335
- RefSeq:   NP_854017.1
- ProteinModelPortal:   P0A4W1
- EnsemblBacteria:   EBMYCT00000016688
- GeneID:   1091234
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb0354c
- GeneTree:   EBGT00050000015723
- HOGENOM:   HBG492579
- OMA:   GQMPGLH
- ProtClustDB:   CLSK2301103
- BioCyc:   MBOV233413:MB0354C-MONOMER
- InterPro:   IPR004841
- InterPro:   IPR002293
- InterPro:   IPR004840
- PANTHER:   PTHR11785
- PIRSF:   PIRSF006060

Pfam domain/function: PF00324 AA_permease

EC number: NA

Molecular weight: Translated: 52195; Mature: 52064

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS00218 AMINO_ACID_PERMEASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1b593bd8)-; HASH(0x1c13e4bc)-; HASH(0x1b5187ec)-; HASH(0x1bd0ccd0)-; HASH(0x1bf8fb50)-; HASH(0x1b48b62c)-; HASH(0x1b9a1bdc)-; HASH(0x1c2ca148)-; HASH(0x1a2d6924)-; HASH(0x1c371c80)-; HASH(0x1b3c89b8)-; HASH(0x18d79438)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPPLDITDERLTREDTGYHKGLHSRQLQMIALGGAIGTGLFLGAGGRLASAGPGLFLVYG
CCCCCCCHHHHHHCCCHHHCCCCCCCEEEEEECCHHHHHHHCCCCCCCCCCCCCHHHHHH
ICGIFVFLILRALGELVLHRPSSGSFVSYAREFYGEKVAFVAGWMYFLNWAMTGIVDTTA
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
IAHYCHYWRAFQPIPQWTLALIALLVVLSMNLISVRLFGELEFWASLIKVIALVTFLIVG
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVFLAGRYKIDGQETGVSLWSSHGGIVPTGLLPIVLVTSGVVFAYAAIELVGIAAGETAE
HHHHHCCEEECCHHHCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PAKIMPRAINSVVLRIACFYVGSTVLLALLLPYTAYKEHVSPFVTFFSKIGIDAAGSVMN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LVVLTAALSSLNAGLYSTGRILRSMAINGSGPRFTAPMSKTGVPYGGILLTAGIGLLGII
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHH
LNAIKPSQAFEIVLHIAATGVIAAWATIVACQLRLHRMANAGQLQRPKFRMPLSPFSGYL
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHH
TLAFLAGVLILMYFDEQHGPWMIAATVIGVPALIGGWYLVRNRVTAVAHHAIDHTKSVAV
HHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VHSADPI
EECCCCH
>Mature Secondary Structure 
PPLDITDERLTREDTGYHKGLHSRQLQMIALGGAIGTGLFLGAGGRLASAGPGLFLVYG
CCCCCCHHHHHHCCCHHHCCCCCCCEEEEEECCHHHHHHHCCCCCCCCCCCCCHHHHHH
ICGIFVFLILRALGELVLHRPSSGSFVSYAREFYGEKVAFVAGWMYFLNWAMTGIVDTTA
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
IAHYCHYWRAFQPIPQWTLALIALLVVLSMNLISVRLFGELEFWASLIKVIALVTFLIVG
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVFLAGRYKIDGQETGVSLWSSHGGIVPTGLLPIVLVTSGVVFAYAAIELVGIAAGETAE
HHHHHCCEEECCHHHCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PAKIMPRAINSVVLRIACFYVGSTVLLALLLPYTAYKEHVSPFVTFFSKIGIDAAGSVMN
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LVVLTAALSSLNAGLYSTGRILRSMAINGSGPRFTAPMSKTGVPYGGILLTAGIGLLGII
HHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHH
LNAIKPSQAFEIVLHIAATGVIAAWATIVACQLRLHRMANAGQLQRPKFRMPLSPFSGYL
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHH
TLAFLAGVLILMYFDEQHGPWMIAATVIGVPALIGGWYLVRNRVTAVAHHAIDHTKSVAV
HHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VHSADPI
EECCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-asparagine [Periplasm]; Proton [Periplasm] [C]

Specific reaction: L-asparagine [Periplasm] + Proton [Periplasm] = Proton [Cytoplasm] + L-asparagine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972