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The map label for this gene is cobQ1

Identifier: 148660020

GI number: 148660020

Start: 307736

End: 309220

Strand: Reverse

Name: cobQ1

Synonym: MRA_0264

Alternate gene names: 148660020

Gene position: 309220-307736 (Counterclockwise)

Preceding gene: 148660021

Following gene: 148660019

Centisome position: 7.0

GC content: 69.49

Gene sequence:

>1485_bases
ATGTCGGGTCTGCTGGTCGCGGGTACGACAAGCGACGCCGGTAAGAGCGCCGTGACCGCGGGGCTGTGCCGCGCGTTGGC
CCGACGCGGTGTGCGGGTGGCGCCGTTTAAGGCGCAGAACATGTCCAACAACTCGATGGTCTGCCGAGGCCCCGACGGCA
CGGGCGTGGAGATCGGTCGGGCGCAATGGGTGCAGGCACTCGCGGCTAGGACCACGCCCGAGGCGGCGATGAACCCGGTC
TTGCTCAAGCCGGCCAGTGATCACCGCAGCCACGTGGTGTTGATGGGCAAGCCCTGGGGCGAGGTGGCGTCGTCAAGCTG
GTGTGCGGGGCGGCGAGCGCTCGCCGAGGCCGCATGCCGCGCCTTCGACGCCCTCGCCGCCCGCTACGACGTCGTTGTCG
CGGAGGGCGCCGGCAGCCCGGCCGAAATCAACCTGCGCGCAGGTGACTACGTCAACATGGGGCTTGCCCGTCATGCGGGA
CTGCCGACCATCGTCGTCGGTGACATCGACCGCGGCGGGGTGTTCGCTGCGTTCCTGGGCACCGTCGCGTTGCTGGCTGC
CGAGGACCAGGCGCTGGTCGCGGGGTTTGTGGTCAATAAGTTTCGGGGCGACTCCGACCTGCTGGCGCCAGGTCTGCGCG
ACCTGGAACGGGTCACCGGGCGCAGGGTGTACGGCACCCTGCCGTGGCACCCCGACCTCTGGCTGGACTCCGAAGATGCC
CTCGACCTACAGGGCCGGCGCGCGGCGGGCACCGGGGCCCGCCGGGTAGCCGTCGTGCGCCTGCCACGAATCAGCAACTT
CACCGATGTCGACGCATTGGGTCTTGAGCCCGACTTGGATGTCGTGTTCGCTTCCGACCCCCGCGCGCTGGACGATGCCG
ATCTGATCGTGTTGCCGGGCACCCGGGCAACGATCGCCGATCTAGCCTGGCTGAGGGCGCGAGACCTAGATCGTGCGCTG
CTGGTGCATGTTGCGGCGGGCAAGCCGCTGCTCGGCATTTGTGGCGGGTTCCAGATGCTGGGCCGGGTGATTCGCGACCC
GTACGGCATCGAAGGTCCGGGAGGGCAGGTGACCGAGGTCGAGGGCTTGGGTTTGCTCGACGTGGAGACCGCATTTTCCC
CCCACAAGGTGCTGCGGCTACCGCGCGGCGAAGGACTCGGTGTGCCCGCGTCGGGGTACGAGATTCACCACGGCCGGATC
ACCCGCGGTGACACCGCCGAGGAGTTCCTCGGCGGCGCGCGCGACGGACCGGTGTTCGGCACCATGTGGCACGGCTCGTT
GGAAGGCGACGCCCTGCGCGAGGCCTTCCTGCGAGAGACGCTCGGCCTCGCCCCGTCGGGCTCATGCTTCCTGGCCGCAC
GCGAGCGCCGCCTCGACCTGCTCGGCGATCTCGTCGAACGACACCTCGACGTCGATGCGCTGCTCAACCTGGCCCGCCAT
GGTTGCCCGCCGACCTTGCCCTTTCTCGCTCCGGGCGCGCCATGA

Upstream 100 bases:

>100_bases
CAACGACGCGTTCGGCGGCAGCCCACGCACGCCAATGATGCCCGGAACCTGGGACACCGACTCAGCGACCCGGGTGGAGT
GAGAAACCTTCGGGAGACGG

Downstream 100 bases:

>100_bases
GGCCGCCCCACCTACGATGACCCGGTGCGGATACTGGTCACTGGCGGCGTCCGCTCGGGAAAGTCCACGCACGCCGAAGC
GTTGTTGGGCGATGCGGCCG

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MSGLLVAGTTSDAGKSAVTAGLCRALARRGVRVAPFKAQNMSNNSMVCRGPDGTGVEIGRAQWVQALAARTTPEAAMNPV
LLKPASDHRSHVVLMGKPWGEVASSSWCAGRRALAEAACRAFDALAARYDVVVAEGAGSPAEINLRAGDYVNMGLARHAG
LPTIVVGDIDRGGVFAAFLGTVALLAAEDQALVAGFVVNKFRGDSDLLAPGLRDLERVTGRRVYGTLPWHPDLWLDSEDA
LDLQGRRAAGTGARRVAVVRLPRISNFTDVDALGLEPDLDVVFASDPRALDDADLIVLPGTRATIADLAWLRARDLDRAL
LVHVAAGKPLLGICGGFQMLGRVIRDPYGIEGPGGQVTEVEGLGLLDVETAFSPHKVLRLPRGEGLGVPASGYEIHHGRI
TRGDTAEEFLGGARDGPVFGTMWHGSLEGDALREAFLRETLGLAPSGSCFLAARERRLDLLGDLVERHLDVDALLNLARH
GCPPTLPFLAPGAP

Sequences:

>Translated_494_residues
MSGLLVAGTTSDAGKSAVTAGLCRALARRGVRVAPFKAQNMSNNSMVCRGPDGTGVEIGRAQWVQALAARTTPEAAMNPV
LLKPASDHRSHVVLMGKPWGEVASSSWCAGRRALAEAACRAFDALAARYDVVVAEGAGSPAEINLRAGDYVNMGLARHAG
LPTIVVGDIDRGGVFAAFLGTVALLAAEDQALVAGFVVNKFRGDSDLLAPGLRDLERVTGRRVYGTLPWHPDLWLDSEDA
LDLQGRRAAGTGARRVAVVRLPRISNFTDVDALGLEPDLDVVFASDPRALDDADLIVLPGTRATIADLAWLRARDLDRAL
LVHVAAGKPLLGICGGFQMLGRVIRDPYGIEGPGGQVTEVEGLGLLDVETAFSPHKVLRLPRGEGLGVPASGYEIHHGRI
TRGDTAEEFLGGARDGPVFGTMWHGSLEGDALREAFLRETLGLAPSGSCFLAARERRLDLLGDLVERHLDVDALLNLARH
GCPPTLPFLAPGAP
>Mature_493_residues
SGLLVAGTTSDAGKSAVTAGLCRALARRGVRVAPFKAQNMSNNSMVCRGPDGTGVEIGRAQWVQALAARTTPEAAMNPVL
LKPASDHRSHVVLMGKPWGEVASSSWCAGRRALAEAACRAFDALAARYDVVVAEGAGSPAEINLRAGDYVNMGLARHAGL
PTIVVGDIDRGGVFAAFLGTVALLAAEDQALVAGFVVNKFRGDSDLLAPGLRDLERVTGRRVYGTLPWHPDLWLDSEDAL
DLQGRRAAGTGARRVAVVRLPRISNFTDVDALGLEPDLDVVFASDPRALDDADLIVLPGTRATIADLAWLRARDLDRALL
VHVAAGKPLLGICGGFQMLGRVIRDPYGIEGPGGQVTEVEGLGLLDVETAFSPHKVLRLPRGEGLGVPASGYEIHHGRIT
RGDTAEEFLGGARDGPVFGTMWHGSLEGDALREAFLRETLGLAPSGSCFLAARERRLDLLGDLVERHLDVDALLNLARHG
CPPTLPFLAPGAP

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBQ_MYCBO (P0A533)

Other databases:

- EMBL:   BX248334
- RefSeq:   NP_853926.1
- ProteinModelPortal:   P0A533
- SMR:   P0A533
- EnsemblBacteria:   EBMYCT00000017238
- GeneID:   1091032
- GenomeReviews:   BX248333_GR
- KEGG:   mbo:Mb0261c
- GeneTree:   EBGT00050000016161
- HOGENOM:   HBG302271
- OMA:   RGDIANM
- ProtClustDB:   PRK00784
- BioCyc:   MBOV233413:MB0261C-MONOMER
- HAMAP:   MF_00028
- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00313

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.10

Molecular weight: Translated: 52135; Mature: 52004

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS51274 GATASE_COBBQ

Important sites: ACT_SITE 334-334 ACT_SITE 424-424

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLLVAGTTSDAGKSAVTAGLCRALARRGVRVAPFKAQNMSNNSMVCRGPDGTGVEIGR
CCCEEEEECCCCCCHHHHHHHHHHHHHHCCCEEECEECCCCCCCCEEEECCCCCCEECHH
AQWVQALAARTTPEAAMNPVLLKPASDHRSHVVLMGKPWGEVASSSWCAGRRALAEAACR
HHHHHHHHHCCCCHHHCCCEEEECCCCCCCEEEEEECCCHHHHCCCHHHHHHHHHHHHHH
AFDALAARYDVVVAEGAGSPAEINLRAGDYVNMGLARHAGLPTIVVGDIDRGGVFAAFLG
HHHHHHHHEEEEEECCCCCCCEEEEECCCEEECCHHHHCCCCEEEEECCCCCCHHHHHHH
TVALLAAEDQALVAGFVVNKFRGDSDLLAPGLRDLERVTGRRVYGTLPWHPDLWLDSEDA
HHHHHHCCCCHHHHHHHHHHHCCCCHHCCCCHHHHHHHCCCEEEEECCCCCCCEECCCCC
LDLQGRRAAGTGARRVAVVRLPRISNFTDVDALGLEPDLDVVFASDPRALDDADLIVLPG
CCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEECC
TRATIADLAWLRARDLDRALLVHVAAGKPLLGICGGFQMLGRVIRDPYGIEGPGGQVTEV
CCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
EGLGLLDVETAFSPHKVLRLPRGEGLGVPASGYEIHHGRITRGDTAEEFLGGARDGPVFG
CCCCEEEECCCCCCHHHEECCCCCCCCCCCCCEEEECCEECCCCCHHHHHCCCCCCCEEE
TMWHGSLEGDALREAFLRETLGLAPSGSCFLAARERRLDLLGDLVERHLDVDALLNLARH
EEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHH
GCPPTLPFLAPGAP
CCCCCCCCCCCCCC
>Mature Secondary Structure 
SGLLVAGTTSDAGKSAVTAGLCRALARRGVRVAPFKAQNMSNNSMVCRGPDGTGVEIGR
CCEEEEECCCCCCHHHHHHHHHHHHHHCCCEEECEECCCCCCCCEEEECCCCCCEECHH
AQWVQALAARTTPEAAMNPVLLKPASDHRSHVVLMGKPWGEVASSSWCAGRRALAEAACR
HHHHHHHHHCCCCHHHCCCEEEECCCCCCCEEEEEECCCHHHHCCCHHHHHHHHHHHHHH
AFDALAARYDVVVAEGAGSPAEINLRAGDYVNMGLARHAGLPTIVVGDIDRGGVFAAFLG
HHHHHHHHEEEEEECCCCCCCEEEEECCCEEECCHHHHCCCCEEEEECCCCCCHHHHHHH
TVALLAAEDQALVAGFVVNKFRGDSDLLAPGLRDLERVTGRRVYGTLPWHPDLWLDSEDA
HHHHHHCCCCHHHHHHHHHHHCCCCHHCCCCHHHHHHHCCCEEEEECCCCCCCEECCCCC
LDLQGRRAAGTGARRVAVVRLPRISNFTDVDALGLEPDLDVVFASDPRALDDADLIVLPG
CCCCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEECC
TRATIADLAWLRARDLDRALLVHVAAGKPLLGICGGFQMLGRVIRDPYGIEGPGGQVTEV
CCHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEE
EGLGLLDVETAFSPHKVLRLPRGEGLGVPASGYEIHHGRITRGDTAEEFLGGARDGPVFG
CCCCEEEECCCCCCHHHEECCCCCCCCCCCCCEEEECCEECCCCCHHHHHCCCCCCCEEE
TMWHGSLEGDALREAFLRETLGLAPSGSCFLAARERRLDLLGDLVERHLDVDALLNLARH
EEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHH
GCPPTLPFLAPGAP
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972