Click here to switch to the map view.

The map label for this gene is echA1 [H]

Identifier: 148659986

GI number: 148659986

Start: 266869

End: 267657

Strand: Direct

Name: echA1 [H]

Synonym: MRA_0230

Alternate gene names: 148659986

Gene position: 266869-267657 (Clockwise)

Preceding gene: 148659985

Following gene: 148659989

Centisome position: 6.04

GC content: 65.15

Gene sequence:

>789_bases
ATGAGCAGCGAAAGCGACGCAGCCAACACCGAACCTGAGGTTCTGGTAGAACAGCGGGATCGGATTTTGATCATCACGAT
CAACCGCCCGAAAGCCAAGAACGCGGTCAACGCCGCAGTCAGCCGGGGCTTGGCCGATGCGATGGATCAGCTTGACGGCG
ATGCCGGCCTGTCGGTGGCAATCCTGACCGGTGGGGGCGGTTCGTTCTGCGCGGGCATGGACCTCAAGGCGTTCGCCCGG
GGCGAGAATGTCGTCGTCGAAGGTCGCGGCCTTGGCTTTACCGAACGTCCGCCGACCAAGCCGCTCATTGCTGCGGTGGA
AGGCTACGCGTTGGCGGGTGGCACCGAGCTGGCGCTTGCTGCCGACCTGATCGTGGCGGCCAGGGATTCGGCGTTCGGGA
TTCCTGAAGTCAAGCGGGGTCTGGTTGCCGGCGGCGGGGGATTGCTGCGGTTGCCGGAGCGCATCCCGTATGCGATAGCC
ATGGAGTTGGCGCTGACCGGTGACAACCTACCGGCCGAACGCGCGCACGAGCTGGGGCTCGTCAACGTTTTGGCCGAGCC
GGGGACCGCCCTCGATGCTGCGATCGCGTTGGCGGAGAAGATCACCGCCAATGGGCCGCTGGCGGTGGTGGCCACCAAGC
GGATTATCACCGAGTCGCGTGGGTGGAGTCCCGACACTATGTTCGCTGAGCAGATGAAGATCCTGGTGCCGGTGTTCACC
TCCAACGACGCGAAGGAAGGTGCGATCGCGTTCGCCGAGAGGCGCCGGCCCCGTTGGACGGGCACCTAG

Upstream 100 bases:

>100_bases
CGAAATACGCCGCGCCGCTGGTCTTTCCGTGGAGTTGACAGTCGTCGAGTCCGCGATGGCGCAGGCATGACACGAAACAC
CGGACGAGTATGAGGCCAGT

Downstream 100 bases:

>100_bases
CCCAGCTACGCGACGGTGTAGCCCATCGGCAGCAGGACACTCTTTTGCTGGGTGAAGTGTTCGACACCCTCGGGCCCGTT
CTCGCGGCCGATTCCGGAGT

Product: enoyl-CoA hydratase

Products: L-Carnitine.; L-carnitinyl-CoA [C]

Alternate protein names: Crotonobetainyl-CoA hydratase [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MSSESDAANTEPEVLVEQRDRILIITINRPKAKNAVNAAVSRGLADAMDQLDGDAGLSVAILTGGGGSFCAGMDLKAFAR
GENVVVEGRGLGFTERPPTKPLIAAVEGYALAGGTELALAADLIVAARDSAFGIPEVKRGLVAGGGGLLRLPERIPYAIA
MELALTGDNLPAERAHELGLVNVLAEPGTALDAAIALAEKITANGPLAVVATKRIITESRGWSPDTMFAEQMKILVPVFT
SNDAKEGAIAFAERRRPRWTGT

Sequences:

>Translated_262_residues
MSSESDAANTEPEVLVEQRDRILIITINRPKAKNAVNAAVSRGLADAMDQLDGDAGLSVAILTGGGGSFCAGMDLKAFAR
GENVVVEGRGLGFTERPPTKPLIAAVEGYALAGGTELALAADLIVAARDSAFGIPEVKRGLVAGGGGLLRLPERIPYAIA
MELALTGDNLPAERAHELGLVNVLAEPGTALDAAIALAEKITANGPLAVVATKRIITESRGWSPDTMFAEQMKILVPVFT
SNDAKEGAIAFAERRRPRWTGT
>Mature_261_residues
SSESDAANTEPEVLVEQRDRILIITINRPKAKNAVNAAVSRGLADAMDQLDGDAGLSVAILTGGGGSFCAGMDLKAFARG
ENVVVEGRGLGFTERPPTKPLIAAVEGYALAGGTELALAADLIVAARDSAFGIPEVKRGLVAGGGGLLRLPERIPYAIAM
ELALTGDNLPAERAHELGLVNVLAEPGTALDAAIALAEKITANGPLAVVATKRIITESRGWSPDTMFAEQMKILVPVFTS
NDAKEGAIAFAERRRPRWTGT

Specific function: Catalyzes the reversible dehydration of L-carnitinyl-CoA to crotonobetainyl-CoA [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=268, Percent_Identity=32.089552238806, Blast_Score=129, Evalue=2e-30,
Organism=Homo sapiens, GI70995211, Length=224, Percent_Identity=33.4821428571429, Blast_Score=99, Evalue=4e-21,
Organism=Homo sapiens, GI4502327, Length=271, Percent_Identity=32.8413284132841, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI20127408, Length=176, Percent_Identity=29.5454545454545, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI150378541, Length=246, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI31542718, Length=250, Percent_Identity=26.4, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI37594469, Length=189, Percent_Identity=30.1587301587302, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI37594471, Length=189, Percent_Identity=30.1587301587302, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI68989263, Length=202, Percent_Identity=26.2376237623762, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI221142681, Length=249, Percent_Identity=41.7670682730924, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1787659, Length=257, Percent_Identity=32.6848249027237, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1787660, Length=253, Percent_Identity=32.8063241106719, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI87082183, Length=257, Percent_Identity=28.0155642023346, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1788597, Length=250, Percent_Identity=27.2, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI1788682, Length=191, Percent_Identity=34.5549738219895, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1790281, Length=171, Percent_Identity=30.9941520467836, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25145438, Length=247, Percent_Identity=36.4372469635627, Blast_Score=154, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17554946, Length=255, Percent_Identity=34.5098039215686, Blast_Score=143, Evalue=8e-35,
Organism=Caenorhabditis elegans, GI17560910, Length=259, Percent_Identity=32.046332046332, Blast_Score=127, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI17540714, Length=220, Percent_Identity=35.9090909090909, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17534483, Length=218, Percent_Identity=28.4403669724771, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17558304, Length=182, Percent_Identity=30.2197802197802, Blast_Score=89, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI25144276, Length=182, Percent_Identity=31.8681318681319, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17508951, Length=182, Percent_Identity=31.8681318681319, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17508953, Length=182, Percent_Identity=31.8681318681319, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17540306, Length=267, Percent_Identity=26.2172284644195, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI25144157, Length=198, Percent_Identity=27.7777777777778, Blast_Score=64, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI25144160, Length=198, Percent_Identity=27.7777777777778, Blast_Score=64, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6320241, Length=185, Percent_Identity=27.5675675675676, Blast_Score=76, Evalue=5e-15,
Organism=Drosophila melanogaster, GI20129971, Length=244, Percent_Identity=36.4754098360656, Blast_Score=137, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24653477, Length=244, Percent_Identity=36.4754098360656, Blast_Score=137, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24653139, Length=263, Percent_Identity=35.7414448669202, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI21357171, Length=260, Percent_Identity=31.1538461538462, Blast_Score=118, Evalue=4e-27,
Organism=Drosophila melanogaster, GI24650670, Length=218, Percent_Identity=35.7798165137615, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI19920382, Length=218, Percent_Identity=27.9816513761468, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI19921000, Length=177, Percent_Identity=37.2881355932203, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24583077, Length=177, Percent_Identity=37.2881355932203, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24583079, Length=177, Percent_Identity=37.2881355932203, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24654903, Length=255, Percent_Identity=25.0980392156863, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19922422, Length=260, Percent_Identity=26.1538461538462, Blast_Score=78, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022852
- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.- [C]

Molecular weight: Translated: 27281; Mature: 27149

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSESDAANTEPEVLVEQRDRILIITINRPKAKNAVNAAVSRGLADAMDQLDGDAGLSVA
CCCCCCCCCCCHHHHEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEE
ILTGGGGSFCAGMDLKAFARGENVVVEGRGLGFTERPPTKPLIAAVEGYALAGGTELALA
EEECCCCCEEECCCHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHCCCEEECCCCHHHHH
ADLIVAARDSAFGIPEVKRGLVAGGGGLLRLPERIPYAIAMELALTGDNLPAERAHELGL
HHHHEEECCCCCCCHHHHHCEEECCCCEEECCCCCCEEEEEEEEEECCCCCHHHHHHCCE
VNVLAEPGTALDAAIALAEKITANGPLAVVATKRIITESRGWSPDTMFAEQMKILVPVFT
EEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEHHHHHCCCCCCCHHHHHHCCEEEEEEEE
SNDAKEGAIAFAERRRPRWTGT
CCCCCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SSESDAANTEPEVLVEQRDRILIITINRPKAKNAVNAAVSRGLADAMDQLDGDAGLSVA
CCCCCCCCCCHHHHEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEE
ILTGGGGSFCAGMDLKAFARGENVVVEGRGLGFTERPPTKPLIAAVEGYALAGGTELALA
EEECCCCCEEECCCHHHHCCCCEEEEECCCCCCCCCCCCCHHHHHCCCEEECCCCHHHHH
ADLIVAARDSAFGIPEVKRGLVAGGGGLLRLPERIPYAIAMELALTGDNLPAERAHELGL
HHHHEEECCCCCCCHHHHHCEEECCCCEEECCCCCCEEEEEEEEEECCCCCHHHHHHCCE
VNVLAEPGTALDAAIALAEKITANGPLAVVATKRIITESRGWSPDTMFAEQMKILVPVFT
EEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEHHHHHCCCCCCCHHHHHHCCEEEEEEEE
SNDAKEGAIAFAERRRPRWTGT
CCCCCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Carnitine; D-carnitinyl-CoA [C]

Specific reaction: D-Carnitine = L-Carnitine. D-carnitinyl-CoA = L-carnitinyl-CoA [C]

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA