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The map label for this gene is 148659985

Identifier: 148659985

GI number: 148659985

Start: 265429

End: 266838

Strand: Direct

Name: 148659985

Synonym: MRA_0229

Alternate gene names: NA

Gene position: 265429-266838 (Clockwise)

Preceding gene: 148659984

Following gene: 148659986

Centisome position: 6.01

GC content: 62.7

Gene sequence:

>1410_bases
GTGAAACGGCTCAGCGGCTGGGACGCGGTACTGCTTTACAGCGAGACCCCGAATGTGCACATGCACACACTCAAGGTCGC
CGTGATCGAATTGGATTCGGACAGACAGGAATTCGGTGTCGACGCGTTTCGCGAGGTGATCGCTGGCCGGCTGCATAAGC
TTGAGCCATTGGGCTATCAGCTGGTTGATGTCCCGTTGAAGTTCCATCACCCGATGTGGCGGGAGCACTGCCAGGTCGAT
CTCAACTACCACATCCGGCCGTGGCGGTTGCGCGCCCCGGGGGGTCGGCGCGAACTCGACGAGGCGGTCGGAGAAATCGC
CAGCACCCCGCTGAACCGCGACCACCCGCTGTGGGAGATGTACTTCGTTGAGGGGCTTGCCAACCACCGGATCGCGGTGG
TTGCCAAAATTCACCATGCGTTGGCTGACGGTGTTGCCTCGGCAAACATGATGGCACGGGGGATGGATCTGCTGCCGGGA
CCGGAGGTCGGCCGCTATGTGCCTGACCCCGCTCCTACCAAGCGGCAGTTGCTGTCCGCGGCGTTCATCGACCACTTGCG
CCACCTCGGCCGGATTCCTGCAACCATCCGGTACACCACGCAGGGTCTAGGCCGGGTGCGACGTAGCTCGCGCAAGCTCT
CACCCGCACTGACCATGCCATTTACCCCGCCACCGACGTTCATGAATCACCGGCTCACCCCGGAGCGCAGGTTCGCCACC
GCCACCCTGGCGCTGATTGACGTGAAGGCGACGGCCAAGTTGCTGGGGGCGACGATCAACGACATGGTGCTGGCCATGTC
GACCGGCGCTCTGCGTACCCTGCTATTGCGCTATGACGGCAAGGCCGAACCGCTGCTGGCGTCGGTCCCGGTGAGTTACG
ACTTCTCACCGGAGCGGATCTCCGGTAACCGCTTCACCGGAATGCTGGTGGCGCTGCCTGCCGACTCCGACGACCCGTTG
CAGCGGGTGCGCGTCTGTCACGAAAACGCGGTCTCCGCCAAGGAGAGCCACCAGCTTTTGGGACCGGAGTTGATCAGCCG
CTGGGCGGCTTACTGGCCACCTGCCGGTGCGGAAGCCTTGTTCCGGTGGTTGTCTGAGCGCGACGGGCAGAACAAGGTAC
TCAACTTGAATATCTCGAATGTTCCCGGTCCGCGCGAACGCGGCCGCGTGGGGGCCGCGCTGGTCACCGAGATCTATTCG
GTGGGCCCGTTGACCGCCGGTAGCGGATTGAATATCACGGTGTGGAGTTATGTCGATCAGCTCAATATCTCGGTGTTAAC
CGATGGTTCCACCGTGCAGGACCCGCATGAAGTAACCGCGGGAATGATCGCGGACTTCATCGAAATACGCCGCGCCGCTG
GTCTTTCCGTGGAGTTGACAGTCGTCGAGTCCGCGATGGCGCAGGCATGA

Upstream 100 bases:

>100_bases
CTGTTTCTCAACCAGGTTCATCGCAGCCGGGCACAGTTCGCGAAAGAGGTCATCTAAACGCCGGCCAATTGTATGGTCGC
CCTATGAGTAGGGGGCTGCG

Downstream 100 bases:

>100_bases
CACGAAACACCGGACGAGTATGAGGCCAGTATGAGCAGCGAAAGCGACGCAGCCAACACCGAACCTGAGGTTCTGGTAGA
ACAGCGGGATCGGATTTTGA

Product: hypothetical protein

Products: NA

Alternate protein names: Putative triacylglycerol synthase Rv0221/MT0231

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MKRLSGWDAVLLYSETPNVHMHTLKVAVIELDSDRQEFGVDAFREVIAGRLHKLEPLGYQLVDVPLKFHHPMWREHCQVD
LNYHIRPWRLRAPGGRRELDEAVGEIASTPLNRDHPLWEMYFVEGLANHRIAVVAKIHHALADGVASANMMARGMDLLPG
PEVGRYVPDPAPTKRQLLSAAFIDHLRHLGRIPATIRYTTQGLGRVRRSSRKLSPALTMPFTPPPTFMNHRLTPERRFAT
ATLALIDVKATAKLLGATINDMVLAMSTGALRTLLLRYDGKAEPLLASVPVSYDFSPERISGNRFTGMLVALPADSDDPL
QRVRVCHENAVSAKESHQLLGPELISRWAAYWPPAGAEALFRWLSERDGQNKVLNLNISNVPGPRERGRVGAALVTEIYS
VGPLTAGSGLNITVWSYVDQLNISVLTDGSTVQDPHEVTAGMIADFIEIRRAAGLSVELTVVESAMAQA

Sequences:

>Translated_469_residues
MKRLSGWDAVLLYSETPNVHMHTLKVAVIELDSDRQEFGVDAFREVIAGRLHKLEPLGYQLVDVPLKFHHPMWREHCQVD
LNYHIRPWRLRAPGGRRELDEAVGEIASTPLNRDHPLWEMYFVEGLANHRIAVVAKIHHALADGVASANMMARGMDLLPG
PEVGRYVPDPAPTKRQLLSAAFIDHLRHLGRIPATIRYTTQGLGRVRRSSRKLSPALTMPFTPPPTFMNHRLTPERRFAT
ATLALIDVKATAKLLGATINDMVLAMSTGALRTLLLRYDGKAEPLLASVPVSYDFSPERISGNRFTGMLVALPADSDDPL
QRVRVCHENAVSAKESHQLLGPELISRWAAYWPPAGAEALFRWLSERDGQNKVLNLNISNVPGPRERGRVGAALVTEIYS
VGPLTAGSGLNITVWSYVDQLNISVLTDGSTVQDPHEVTAGMIADFIEIRRAAGLSVELTVVESAMAQA
>Mature_469_residues
MKRLSGWDAVLLYSETPNVHMHTLKVAVIELDSDRQEFGVDAFREVIAGRLHKLEPLGYQLVDVPLKFHHPMWREHCQVD
LNYHIRPWRLRAPGGRRELDEAVGEIASTPLNRDHPLWEMYFVEGLANHRIAVVAKIHHALADGVASANMMARGMDLLPG
PEVGRYVPDPAPTKRQLLSAAFIDHLRHLGRIPATIRYTTQGLGRVRRSSRKLSPALTMPFTPPPTFMNHRLTPERRFAT
ATLALIDVKATAKLLGATINDMVLAMSTGALRTLLLRYDGKAEPLLASVPVSYDFSPERISGNRFTGMLVALPADSDDPL
QRVRVCHENAVSAKESHQLLGPELISRWAAYWPPAGAEALFRWLSERDGQNKVLNLNISNVPGPRERGRVGAALVTEIYS
VGPLTAGSGLNITVWSYVDQLNISVLTDGSTVQDPHEVTAGMIADFIEIRRAAGLSVELTVVESAMAQA

Specific function: Upon expression in E.coli functions weakly as a triacylglycerol synthase, making triacylglycerol (TG) from diolein and long-chain fatty acyl-CoA. Has very weak wax synthase activity, incorporating palmityl alcohol into wax esters in the presence of palmit

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the long-chain O-acyltransferase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): Y221_MYCTU (P96403)

Other databases:

- EMBL:   BX842572
- EMBL:   AE000516
- PIR:   C70961
- RefSeq:   NP_214735.1
- RefSeq:   NP_334638.1
- ProteinModelPortal:   P96403
- EnsemblBacteria:   EBMYCT00000003284
- EnsemblBacteria:   EBMYCT00000070484
- GeneID:   886719
- GeneID:   923127
- GenomeReviews:   AE000516_GR
- GenomeReviews:   AL123456_GR
- KEGG:   mtc:MT0231
- KEGG:   mtu:Rv0221
- TIGR:   MT0231
- TubercuList:   Rv0221
- GeneTree:   EBGT00050000015226
- HOGENOM:   HBG696081
- OMA:   HEVTEAM
- ProtClustDB:   CLSK790360
- InterPro:   IPR014292
- InterPro:   IPR009721
- InterPro:   IPR004255
- TIGRFAMs:   TIGR02946

Pfam domain/function: PF06974 DUF1298; PF03007 UPF0089

EC number: =2.3.1.20

Molecular weight: Translated: 51862; Mature: 51862

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: NA

Important sites: ACT_SITE 139-139

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLSGWDAVLLYSETPNVHMHTLKVAVIELDSDRQEFGVDAFREVIAGRLHKLEPLGYQ
CCCCCCCCEEEEEECCCCEEEEEEEEEEEEECCCHHHHCHHHHHHHHHHHHHHCCCCCCE
LVDVPLKFHHPMWREHCQVDLNYHIRPWRLRAPGGRRELDEAVGEIASTPLNRDHPLWEM
EEECCHHHCCCHHHHHCEEEEEEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHH
YFVEGLANHRIAVVAKIHHALADGVASANMMARGMDLLPGPEVGRYVPDPAPTKRQLLSA
HHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
AFIDHLRHLGRIPATIRYTTQGLGRVRRSSRKLSPALTMPFTPPPTFMNHRLTPERRFAT
HHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCCEEECCCCCCCHHHCCCCCCHHHHHE
ATLALIDVKATAKLLGATINDMVLAMSTGALRTLLLRYDGKAEPLLASVPVSYDFSPERI
EEEEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCC
SGNRFTGMLVALPADSDDPLQRVRVCHENAVSAKESHQLLGPELISRWAAYWPPAGAEAL
CCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHCCCCCHHHH
FRWLSERDGQNKVLNLNISNVPGPRERGRVGAALVTEIYSVGPLTAGSGLNITVWSYVDQ
HHHHHHCCCCCCEEEEEECCCCCCHHHCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCC
LNISVLTDGSTVQDPHEVTAGMIADFIEIRRAAGLSVELTVVESAMAQA
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHCC
>Mature Secondary Structure
MKRLSGWDAVLLYSETPNVHMHTLKVAVIELDSDRQEFGVDAFREVIAGRLHKLEPLGYQ
CCCCCCCCEEEEEECCCCEEEEEEEEEEEEECCCHHHHCHHHHHHHHHHHHHHCCCCCCE
LVDVPLKFHHPMWREHCQVDLNYHIRPWRLRAPGGRRELDEAVGEIASTPLNRDHPLWEM
EEECCHHHCCCHHHHHCEEEEEEEEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCHHHH
YFVEGLANHRIAVVAKIHHALADGVASANMMARGMDLLPGPEVGRYVPDPAPTKRQLLSA
HHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHH
AFIDHLRHLGRIPATIRYTTQGLGRVRRSSRKLSPALTMPFTPPPTFMNHRLTPERRFAT
HHHHHHHHHCCCCCEEEEHHHHHHHHHHHHCCCCCEEECCCCCCCHHHCCCCCCHHHHHE
ATLALIDVKATAKLLGATINDMVLAMSTGALRTLLLRYDGKAEPLLASVPVSYDFSPERI
EEEEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCC
SGNRFTGMLVALPADSDDPLQRVRVCHENAVSAKESHQLLGPELISRWAAYWPPAGAEAL
CCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHCCCCCHHHH
FRWLSERDGQNKVLNLNISNVPGPRERGRVGAALVTEIYSVGPLTAGSGLNITVWSYVDQ
HHHHHHCCCCCCEEEEEECCCCCCHHHCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCC
LNISVLTDGSTVQDPHEVTAGMIADFIEIRRAAGLSVELTVVESAMAQA
CEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036