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The map label for this gene is adhE1 [H]

Identifier: 148659926

GI number: 148659926

Start: 193344

End: 194495

Strand: Reverse

Name: adhE1 [H]

Synonym: MRA_0170

Alternate gene names: 148659926

Gene position: 194495-193344 (Counterclockwise)

Preceding gene: 148659929

Following gene: 148659924

Centisome position: 4.4

GC content: 67.62

Gene sequence:

>1152_bases
GTGCCGGCAGTGCAGCCATGGTTGTATTCCAACATGCCTGCCATCCGGGGCGCGGTGCTAGACCAGATCGGTGTGCCGCG
GCCCTATTGGCGGTCGAAACCAATCAGCGTCGTCGAGCTGCACCTGGACCCGCCCGACCGCGGTGAGGTCCTTGTCCGAA
TCGAGGCTGCGGGCGTGTGCCACTCCGACCTGTCGGTGGTCGACGGCACCCGGGTACGGCCGGTGCCGATCTTGCTCGGT
CATGAAGCCGCTGGAATCGTCGAGCAGGTCGGCGACGGCGTCGACGGGGTCGCGGTCGGTCAGCGAGTCGTGCTGGTGTT
CCTTCCGCGTTGCGGCCAGTGCGCGGCGTGCGCGACCGACGGTCGGACGCCGTGCGAACCGGGCAGCGCGGCCAACAAGG
CCGGCACACTGCTCGGTGGCGGTATCCGGCTCAGCCGGGGCGGCCGCCCGGTGTACCACCACCTCGGCGTCTCGGGTTTC
GCGACCCATGTCGTCGTCAACCGGGCCAGCGTGGTTCCGGTGCCGCACGAGGTGCCGCCCACCGTCGCTGCCCTACTCGG
GTGCGCGGTGCTCACCGGTGGGGGTGCGGTACTCAACGTCGGTGATCCGCAGCCCGGCCAGTCGGTCGCCGTCGTCGGCC
TCGGGGGCGTCGGTATGGCAGCGGTGCTCACCGCTTTGACGTATACAGACGTTCGTGTGGTCGCCGTCGATCAGCTACCC
GAAAAGCTGTCGGCCGCAAAGGCCTTGGGCGCCCATGAGATCTACACACCGCAGCAGGCCACGGCCGGCGGGGTCAAAGC
CGCCGTGGTTGTCGAAGCCGTCGGTCATCCTGCCGCGCTGCACACCGCGATCGGGCTTACCGCGCCCGGAGGGCGCACCA
TCACCGTCGGGTTGCCGCCACCGGATGTCCGGATCAGCTTGTCGCCGTTGGATTTTGTCACCGAAGGCCGGTCACTGATC
GGCAGCTATCTGGGTTCGGCGGTGCCCAGCCACGACATTCCCCGCTTCGTGTCGTTGTGGCAATCGGGCCGTCTGCCGGT
GGAGTCGCTGGTGACGTCAACGATTCGGTTGGACGATATCAACGAGGCGATGGACCACCTCGCCGATGGCATCGCGGTGC
GTCAGCTCATCAGTTTCACCGGTGATCTCTAA

Upstream 100 bases:

>100_bases
TAGTAGACGGCGTTGTTGAGGTGGCCGAACATGTCGTTGTCGGCCCATCGAGTTCCCACCGGCCACAGCACCGGAAAGTC
GCTGCGCAGGAGCTTCTCCG

Downstream 100 bases:

>100_bases
AGGCGCTGAATGCTTGCGCAGCACCGGTCAGTCGAACAAGACCGCGGCGTTAAGGTACCCGGTCGGGTCCAACGCCGCCT
TGACCCTCCGCATCGCGGCG

Product: zinc-type alcohol dehydrogenase subunit E

Products: NA

Alternate protein names: Alcohol dehydrogenase class-3; Alcohol dehydrogenase class-III; Glutathione-dependent formaldehyde dehydrogenase; FALDH; FDH; GSH-FDH [H]

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MPAVQPWLYSNMPAIRGAVLDQIGVPRPYWRSKPISVVELHLDPPDRGEVLVRIEAAGVCHSDLSVVDGTRVRPVPILLG
HEAAGIVEQVGDGVDGVAVGQRVVLVFLPRCGQCAACATDGRTPCEPGSAANKAGTLLGGGIRLSRGGRPVYHHLGVSGF
ATHVVVNRASVVPVPHEVPPTVAALLGCAVLTGGGAVLNVGDPQPGQSVAVVGLGGVGMAAVLTALTYTDVRVVAVDQLP
EKLSAAKALGAHEIYTPQQATAGGVKAAVVVEAVGHPAALHTAIGLTAPGGRTITVGLPPPDVRISLSPLDFVTEGRSLI
GSYLGSAVPSHDIPRFVSLWQSGRLPVESLVTSTIRLDDINEAMDHLADGIAVRQLISFTGDL

Sequences:

>Translated_383_residues
MPAVQPWLYSNMPAIRGAVLDQIGVPRPYWRSKPISVVELHLDPPDRGEVLVRIEAAGVCHSDLSVVDGTRVRPVPILLG
HEAAGIVEQVGDGVDGVAVGQRVVLVFLPRCGQCAACATDGRTPCEPGSAANKAGTLLGGGIRLSRGGRPVYHHLGVSGF
ATHVVVNRASVVPVPHEVPPTVAALLGCAVLTGGGAVLNVGDPQPGQSVAVVGLGGVGMAAVLTALTYTDVRVVAVDQLP
EKLSAAKALGAHEIYTPQQATAGGVKAAVVVEAVGHPAALHTAIGLTAPGGRTITVGLPPPDVRISLSPLDFVTEGRSLI
GSYLGSAVPSHDIPRFVSLWQSGRLPVESLVTSTIRLDDINEAMDHLADGIAVRQLISFTGDL
>Mature_382_residues
PAVQPWLYSNMPAIRGAVLDQIGVPRPYWRSKPISVVELHLDPPDRGEVLVRIEAAGVCHSDLSVVDGTRVRPVPILLGH
EAAGIVEQVGDGVDGVAVGQRVVLVFLPRCGQCAACATDGRTPCEPGSAANKAGTLLGGGIRLSRGGRPVYHHLGVSGFA
THVVVNRASVVPVPHEVPPTVAALLGCAVLTGGGAVLNVGDPQPGQSVAVVGLGGVGMAAVLTALTYTDVRVVAVDQLPE
KLSAAKALGAHEIYTPQQATAGGVKAAVVVEAVGHPAALHTAIGLTAPGGRTITVGLPPPDVRISLSPLDFVTEGRSLIG
SYLGSAVPSHDIPRFVSLWQSGRLPVESLVTSTIRLDDINEAMDHLADGIAVRQLISFTGDL

Specific function: Has High Formaldehyde Dehydrogenase Activity In The Presence Of Glutathione And Catalyzes The Oxidation Of Normal Alcohols In A Reaction That Is Not Gsh-Dependent. In Addition, Hemithiolacetals Other Than Those Formed From Gsh, Including Omega-Thiol Fatty

COG id: COG1062

COG function: function code C; Zn-dependent alcohol dehydrogenases, class III

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily [H]

Homologues:

Organism=Homo sapiens, GI34577061, Length=363, Percent_Identity=34.1597796143251, Blast_Score=220, Evalue=2e-57,
Organism=Homo sapiens, GI4501933, Length=363, Percent_Identity=33.8842975206612, Blast_Score=220, Evalue=2e-57,
Organism=Homo sapiens, GI4501929, Length=363, Percent_Identity=33.6088154269972, Blast_Score=216, Evalue=3e-56,
Organism=Homo sapiens, GI71743840, Length=361, Percent_Identity=30.7479224376731, Blast_Score=202, Evalue=3e-52,
Organism=Homo sapiens, GI262073058, Length=361, Percent_Identity=30.7479224376731, Blast_Score=202, Evalue=3e-52,
Organism=Homo sapiens, GI156523966, Length=362, Percent_Identity=31.4917127071823, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI71565154, Length=363, Percent_Identity=30.8539944903581, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI4501939, Length=355, Percent_Identity=31.830985915493, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI71565152, Length=368, Percent_Identity=30.7065217391304, Blast_Score=182, Evalue=4e-46,
Organism=Escherichia coli, GI1786552, Length=359, Percent_Identity=33.1476323119777, Blast_Score=174, Evalue=9e-45,
Organism=Escherichia coli, GI1787863, Length=356, Percent_Identity=25.561797752809, Blast_Score=101, Evalue=9e-23,
Organism=Escherichia coli, GI87081918, Length=362, Percent_Identity=28.1767955801105, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788407, Length=333, Percent_Identity=23.7237237237237, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87082125, Length=367, Percent_Identity=24.7956403269755, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1788075, Length=349, Percent_Identity=25.7879656160458, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1790045, Length=347, Percent_Identity=24.4956772334294, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI226510992, Length=357, Percent_Identity=24.6498599439776, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1786825, Length=265, Percent_Identity=30.188679245283, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1790718, Length=350, Percent_Identity=24.5714285714286, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788073, Length=348, Percent_Identity=24.7126436781609, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI87082401, Length=353, Percent_Identity=25.4957507082153, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI25146526, Length=366, Percent_Identity=31.4207650273224, Blast_Score=187, Evalue=7e-48,
Organism=Caenorhabditis elegans, GI71997431, Length=368, Percent_Identity=30.9782608695652, Blast_Score=184, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI17565904, Length=357, Percent_Identity=30.5322128851541, Blast_Score=168, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI17562582, Length=369, Percent_Identity=24.6612466124661, Blast_Score=73, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71988145, Length=355, Percent_Identity=25.6338028169014, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6320033, Length=367, Percent_Identity=30.7901907356948, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6323729, Length=311, Percent_Identity=29.2604501607717, Blast_Score=76, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6319258, Length=366, Percent_Identity=24.3169398907104, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319257, Length=358, Percent_Identity=24.5810055865922, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323961, Length=307, Percent_Identity=26.3843648208469, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324486, Length=306, Percent_Identity=25.8169934640523, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319621, Length=305, Percent_Identity=25.9016393442623, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17737895, Length=364, Percent_Identity=30.7692307692308, Blast_Score=186, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014183
- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.284; =1.1.1.1 [H]

Molecular weight: Translated: 39366; Mature: 39235

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAVQPWLYSNMPAIRGAVLDQIGVPRPYWRSKPISVVELHLDPPDRGEVLVRIEAAGVC
CCCCCCHHHCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEECCCC
HSDLSVVDGTRVRPVPILLGHEAAGIVEQVGDGVDGVAVGQRVVLVFLPRCGQCAACATD
CCCCHHCCCCCCCCEEEEECCCHHHHHHHHCCCCCCHHHCCEEEEEEECCCCCCCEECCC
GRTPCEPGSAANKAGTLLGGGIRLSRGGRPVYHHLGVSGFATHVVVNRASVVPVPHEVPP
CCCCCCCCCCCCCCCEEEECCEEECCCCCCEEECCCCCHHHHHHEEECCEECCCCCCCCH
TVAALLGCAVLTGGGAVLNVGDPQPGQSVAVVGLGGVGMAAVLTALTYTDVRVVAVDQLP
HHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEEECCHHHHHHHHHHHHCCEEEEEHHHHH
EKLSAAKALGAHEIYTPQQATAGGVKAAVVVEAVGHPAALHTAIGLTAPGGRTITVGLPP
HHHHHHHHCCCHHCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHEEECCCCCEEEEECCC
PDVRISLSPLDFVTEGRSLIGSYLGSAVPSHDIPRFVSLWQSGRLPVESLVTSTIRLDDI
CCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHEECHH
NEAMDHLADGIAVRQLISFTGDL
HHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PAVQPWLYSNMPAIRGAVLDQIGVPRPYWRSKPISVVELHLDPPDRGEVLVRIEAAGVC
CCCCCHHHCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEECCCC
HSDLSVVDGTRVRPVPILLGHEAAGIVEQVGDGVDGVAVGQRVVLVFLPRCGQCAACATD
CCCCHHCCCCCCCCEEEEECCCHHHHHHHHCCCCCCHHHCCEEEEEEECCCCCCCEECCC
GRTPCEPGSAANKAGTLLGGGIRLSRGGRPVYHHLGVSGFATHVVVNRASVVPVPHEVPP
CCCCCCCCCCCCCCCEEEECCEEECCCCCCEEECCCCCHHHHHHEEECCEECCCCCCCCH
TVAALLGCAVLTGGGAVLNVGDPQPGQSVAVVGLGGVGMAAVLTALTYTDVRVVAVDQLP
HHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEEECCHHHHHHHHHHHHCCEEEEEHHHHH
EKLSAAKALGAHEIYTPQQATAGGVKAAVVVEAVGHPAALHTAIGLTAPGGRTITVGLPP
HHHHHHHHCCCHHCCCCCCCCCCCCEEEEEEEECCCCHHHHHHHEEECCCCCEEEEECCC
PDVRISLSPLDFVTEGRSLIGSYLGSAVPSHDIPRFVSLWQSGRLPVESLVTSTIRLDDI
CCCEEEECCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHEECHH
NEAMDHLADGIAVRQLISFTGDL
HHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]